We narrowed to 2,685 results for: PREP

1. TMEM16A-E551K-peGFP-N1

   Plasmid

   #228584

   Purpose

   Expresses mouse TMEM16A-E551K-eGFP in mammalian cells

   Depositor

   Huanghe Yang

   Article

   Lowry et al bioRxiv [Preprint]. 2024 Sep 3:2024.02.0

   Insert

   TMEM16A/anoctamin 1 (Ano1), transcript variant 2 (Ano1 Mouse)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   E551K

   Promoter

   Available since

   Dec. 2, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pDONR221-DXO

   Plasmid

   #177992

   Purpose

   For gateway cloning of DXO

   Depositor

   Karla Feijs

   Article

   Weixler et al Life Sci Alliance. 2022 Nov 11;6(1):e202

   Insert

   DXO (DXO Human)
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Nov. 11, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pDONR221-PARP11

   Plasmid

   #184469

   Purpose

   For gateway cloning of PARP11

   Depositor

   Karla Feijs

   Article

   Weixler et al Life Sci Alliance. 2022 Nov 11;6(1):e202

   Insert

   PARP11 (PARP11 Human)
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Nov. 4, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pTBL435

   Plasmid

   #226628

   Purpose

   pcDNA-dTdT

   Depositor

   Chang Liu

   Article

   Carlson et al bioRxiv [Preprint]. 2024 Jun 11:2024.06.

   Insert

   catalytically deactivated TdT (DNTT Human)
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mammalian

   Mutation

   D343E, D345E

   Promoter

   CMV

   Available since

   Oct. 23, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. p3E-EF1a-pA (JDW 1222)

   Plasmid

   #224526

   Purpose

   A Gateway compatible 3' entry clone containing rat EF-1a polyA

   Depositor

   Joshua Wythe

   Article

   Gillespie et al bioRxiv [Preprint]. 2024 Jul 13:2024.07.

   Insert

   EF-1a polyA
   

   Use

   Gateway cloning

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Oct. 23, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. p5E_hs_Glast (JDW 1182)

   Plasmid

   #224483

   Purpose

   Gateway compatible 5' entry clone containing the Human GLAST promoter

   Depositor

   Joshua Wythe

   Article

   Gillespie et al bioRxiv [Preprint]. 2024 Jul 13:2024.07.

   Insert

   GLAST promoter
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 7, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. p5E-hs_Cldn5 (JDW 1120)

   Plasmid

   #224481

   Purpose

   Gateway compatible 5' entry clone containing the Human Claudin 5 promoter

   Depositor

   Joshua Wythe

   Article

   Gillespie et al bioRxiv [Preprint]. 2024 Jul 13:2024.07.

   Insert

   Claudin-5 promoter
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Sept. 30, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pME AmCyan (JDW 1151)

   Plasmid

   #224485

   Purpose

   Gateway compatible middle entry clone containing AmCyan cytosolic blue flourescent reporter

   Depositor

   Joshua Wythe

   Article

   Gillespie et al bioRxiv [Preprint]. 2024 Jul 13:2024.07.

   Insert

   AmCyan
   

   Use

   Gateway cloning

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Sept. 30, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pME V5 mScarlet-I (JDW 1323)

   Plasmid

   #224487

   Purpose

   Gateway compatible middle entry clone containing V5 tagged m-Scarlet-I (Cytosolic far red fluorescent reporter)

   Depositor

   Joshua Wythe

   Article

   Gillespie et al bioRxiv [Preprint]. 2024 Jul 13:2024.07.

   Insert

   V5-mScarlet-I
   

   Use

   Gateway cloning

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Sept. 30, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pME-LexA-mODC (no LexOp) (JDW 937)

    Plasmid

    #224504

    Purpose

    Gateway compatible middle entry clone containing a destabilized LexA transactivator

    Depositor

    Joshua Wythe

    Article

    Gillespie et al bioRxiv [Preprint]. 2024 Jul 13:2024.07.

    Insert

    LexA-mODC
    

    Use

    Gateway cloning

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 27, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pME-myc-BirA (JDW 1210)

    Plasmid

    #224509

    Purpose

    Gateway compatible middle entry clone containing a Myc tagged BirA

    Depositor

    Joshua Wythe

    Article

    Gillespie et al bioRxiv [Preprint]. 2024 Jul 13:2024.07.

    Insert

    Myc-BirA
    

    Use

    Gateway cloning

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. p3E-V5-mClover3-pA (JDW 1318)

    Plasmid

    #224530

    Purpose

    A Gateway compatible 3' entry clone containing a V5 tagged mClover3 followed by a polyA

    Depositor

    Joshua Wythe

    Article

    Gillespie et al bioRxiv [Preprint]. 2024 Jul 13:2024.07.

    Insert

    V5-mClover3-pA
    

    Use

    Gateway cloning

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. p3E-V5-mTagBFP2 (JDW 871)

    Plasmid

    #224531

    Purpose

    A Gateway compatible 3' entry clone containing a V5 tagged mTagBFP2

    Depositor

    Joshua Wythe

    Article

    Gillespie et al bioRxiv [Preprint]. 2024 Jul 13:2024.07.

    Insert

    V5-mTagBFP2
    

    Use

    Gateway cloning

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. p3E-IRES-(n2)StayGold(c4)-pA (JDW 1364)

    Plasmid

    #224537

    Purpose

    A Gateway compatible 3' entry clone containing IRES-(n2)StayGold(c4)-pA

    Depositor

    Joshua Wythe

    Article

    Gillespie et al bioRxiv [Preprint]. 2024 Jul 13:2024.07.

    Insert

    IRES-(n2)StayGold(c4)-pA
    

    Use

    Gateway cloning

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. pBK1302-AAV-EFSNC-dCjCas9-KRAB

    Plasmid

    #223147

    Purpose

    Expression of KRAB with dCjCas9 and empty gRNA scaffold

    Depositor

    Boris Kantor

    Article

    Kantor et al bioRxiv [Preprint]. 2024 May 19:2023.04.

    Insert

    KRAB
    

    Use

    AAV and CRISPR

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    Aug. 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. pBK1312-AAV-EFSNC-dSaCas9-KRAB

    Plasmid

    #223157

    Purpose

    Expression of KRAB with dSaCas9 and empty gRNA scaffold

    Depositor

    Boris Kantor

    Article

    Kantor et al bioRxiv [Preprint]. 2024 May 19:2023.04.

    Insert

    KRAB
    

    Use

    AAV and CRISPR

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    Aug. 22, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. pBK2043-AAV-2xSp1-2xNFkB-EFSNC-dSaCas9

    Plasmid

    #223163

    Purpose

    2nd gen. AAV backbone for dSaCas9 (endonuclease dead Cas9 from Staphylococcus aureus) and Sa-gRNA Scaffold

    Depositor

    Boris Kantor

    Article

    Kantor et al bioRxiv [Preprint]. 2024 May 19:2023.04.

    Type

    Empty backbone

    Use

    AAV and CRISPR

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    Encodes aa 204-310 (MECP2)

    Promoter

    Available since

    Aug. 22, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. pPB-EF1A-Puro-hTBK1-E696K-EGFP

    Plasmid

    #221554

    Purpose

    Expresses GFP-tagged human TBK1 with E696K mutation in mammalian cells. Compatible with piggybac transposase for genome integration.

    Depositor

    Shawn Ferguson

    Article

    Talaia et al bioRxiv [Preprint]. 2023 Dec 17:2023.12.

    Insert

    TBK1 (TBK1 Human)
    

    Use

    Piggybac

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Changed glutamate 696 to lysine

    Promoter

    EF1a

    Available since

    July 15, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. pNeonGreen-C1-Nir1-631-894

    Plasmid

    #220089

    Purpose

    Truncated Nir1-LNS2 domain lacking N-terminal amphipathic helix; loses ability to bind PA

    Depositor

    Gerry Hammond

    Article

    Weckerly et al bioRxiv [Preprint]. 2024 Feb 28:2024.02.

    Insert

    PITPNM3 (PITPNM3 Human)
    

    Use

    Tags

    NeonGreen-GGSGG

    Expression

    Mammalian

    Mutation

    amino acids 631-894

    Promoter

    CMV

    Available since

    June 13, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. Efg1-ΔDBD-A51P/A56P/Q70P/Q443P

    Plasmid

    #216403

    Purpose

    Expresses 6xHis-tagged N- and C-terminal prion-like domains of Efg1 with A51/A56/Q70P/Q443 to proline mutations

    Depositor

    Nicolas Fawzi

    Article

    Wang et al bioRxiv [Preprint]. 2023 Nov 9:2023.11.0

    Insert

    Efg1-ΔDBD-A51P/A56P/Q70P/Q443P
    

    Use

    Tags

    6xHis-tags

    Expression

    Bacterial

    Mutation

    changed Alanine 51, 56 and Glutamine 70, 443 to P…

    Promoter

    Available since

    April 17, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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