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pET24a_thyA_R127A_untagged

Plasmid

#191309

Purpose

Bacterial expression of E. coli thyA (thymidylate synthase)

Depositor

Kimberly Reynolds

Article

Nguyen et al Nat Commun. 2024 Apr 18;15(1):3351. doi:

Insert

thyA

Expression

Bacterial

Mutation

R127A TYMS

Promoter

T7

Available Since

June 5, 2023

Availability

Academic Institutions and Nonprofits only

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pET24a_thyA_WT_untagged

Plasmid

#191306

Purpose

Bacterial expression of E. coli thyA (thymidylate synthase)

Depositor

Kimberly Reynolds

Article

Nguyen et al Nat Commun. 2024 Apr 18;15(1):3351. doi:

Insert

thyA

Expression

Bacterial

Mutation

WT TYMS

Promoter

T7

Available Since

June 5, 2023

Availability

Academic Institutions and Nonprofits only

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pT2MPEF-NLS-QUE37C

Plasmid

#129326

Purpose

ATP measurement, nucleus, Tol2 transposon

Depositor

Yasushi Okada

Article

Yaginuma et al bioRxiv 2021.10.08.463131

Insert

QUEEN-37C

Expression

Mammalian

Available Since

Oct. 15, 2021

Availability

Academic Institutions and Nonprofits only
pTrcECT

Plasmid

#75477

Purpose

Inducible expression of ectABC in E. coli

Depositor

Xixian Xie

Article

Ning et al Metab Eng. 2016 Mar 9;36:10-18. doi: 10.

Insert

ectABC

Expression

Bacterial

Promoter

trc

Available Since

May 17, 2016

Availability

Academic Institutions and Nonprofits only

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pATT-Dest

Plasmid

#79770

Purpose

Staging plasmid for use in DIP transposition

Depositor

David Savage

Article

Nadler et al Nat Commun. 2016 Jul 29;7:12266. doi: 10

Type

Empty backbone

Use

Synthetic Biology; Staging plasmid for transposit…

Available Since

July 13, 2016

Availability

Academic Institutions and Nonprofits only

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pUCKanR-Mu-BsaI

Plasmid

#79769

Purpose

Transposon-propagation plasmid for Mu-BsaI modified transposon

Depositor

David Savage

Article

Nadler et al Nat Commun. 2016 Jul 29;7:12266. doi: 10

Insert

Mu-BsaI

Available Since

July 13, 2016

Availability

Academic Institutions and Nonprofits only

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pTKEI-Dest

Plasmid

#79784

Purpose

Expression plasmid - BsmBI entry sites

Depositor

David Savage

Article

Nadler et al Nat Commun. 2016 Jul 29;7:12266. doi: 10

Type

Empty backbone

Tags

6xHis

Expression

Bacterial

Promoter

pTrc

Available Since

July 13, 2016

Availability

Academic Institutions and Nonprofits only

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pStA1BZ

Plasmid

#114164

Purpose

Start-Stop Assembly Level 1BZ plasmid

Depositor

John Heap

Article

Taylor et al Nucleic Acids Res. 2018 Nov 20. pii: 519

Type

Empty backbone

Use

Synthetic Biology

Available Since

March 20, 2019

Availability

Academic Institutions and Nonprofits only

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pStA1BC

Plasmid

#114165

Purpose

Start-Stop Assembly Level 1BC plasmid

Depositor

John Heap

Article

Taylor et al Nucleic Acids Res. 2018 Nov 20. pii: 519

Type

Empty backbone

Use

Synthetic Biology

Available Since

March 20, 2019

Availability

Academic Institutions and Nonprofits only

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pTGD

Plasmid

#88894

Purpose

Tandem gene duplication cassette for RecA mediated homologous recombination in E. coli

Depositor

Keith Tyo

Article

Love et al Methods Mol Biol. 2019;1927:37-45. doi:

Type

Empty backbone

Use

Synthetic Biology

Expression

Bacterial

Available Since

March 29, 2017

Availability

Academic Institutions and Nonprofits only

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pStA212

Plasmid

#114171

Purpose

Start-Stop Assembly Level 212 plasmid

Depositor

John Heap

Article

Taylor et al Nucleic Acids Res. 2018 Nov 20. pii: 519

Type

Empty backbone

Use

Synthetic Biology

Available Since

Nov. 27, 2018

Availability

Academic Institutions and Nonprofits only

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pZ8-Prp

Plasmid

#74065

Purpose

pZ8-1 with the PprpD2 propionate-inducible promoter instead of Ptac, KanR

Depositor

Timothy Lu

Article

Cleto et al ACS Synth Biol. 2016 Feb 16.

Insert

Propionate inducible promoter

Use

Synthetic Biology

Expression

Bacterial

Promoter

prp

Available Since

April 11, 2016

Availability

Academic Institutions and Nonprofits only

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pStA1CZ

Plasmid

#114166

Purpose

Start-Stop Assembly Level 1CZ plasmid

Depositor

John Heap

Article

Taylor et al Nucleic Acids Res. 2018 Nov 20. pii: 519

Type

Empty backbone

Use

Synthetic Biology

Available Since

March 20, 2019

Availability

Academic Institutions and Nonprofits only

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pXP741

Plasmid

#46059

Depositor

Nancy DaSilva

Article

Shen et al Yeast. 2012 Dec;29(12):495-503. doi: 10.

Type

Empty backbone

Expression

Yeast

Promoter

ADH2

Available Since

Sept. 10, 2013

Availability

Academic Institutions and Nonprofits only

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BB1_12_YlpGPD

Plasmid

#117820

Purpose

Promoter pGPD of Y. lipolytica for GoldenMOCS

Depositor

Michael Sauer

Article

Egermeier et al FEMS Microbiol Lett. 2019 Jan 29. pii: 5

Insert

YlpGPD

Expression

Bacterial

Available Since

Feb. 13, 2019

Availability

Academic Institutions and Nonprofits only

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pXP742

Plasmid

#46060

Depositor

Nancy DaSilva

Article

Shen et al Yeast. 2012 Dec;29(12):495-503. doi: 10.

Type

Empty backbone

Expression

Yeast

Promoter

ADH2

Available Since

Sept. 10, 2013

Availability

Academic Institutions and Nonprofits only

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pXP732

Plasmid

#46058

Depositor

Nancy DaSilva

Article

Shen et al Yeast. 2012 Dec;29(12):495-503. doi: 10.

Type

Empty backbone

Expression

Yeast

Promoter

CUP1

Available Since

Sept. 10, 2013

Availability

Academic Institutions and Nonprofits only

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pXP722

Plasmid

#46056

Depositor

Nancy DaSilva

Article

Shen et al Yeast. 2012 Dec;29(12):495-503. doi: 10.

Type

Empty backbone

Expression

Yeast

Promoter

GAL1

Available Since

Sept. 10, 2013

Availability

Academic Institutions and Nonprofits only

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rpaA-

Plasmid

#101847

Purpose

deletes rpaA

Depositor

Erin O'Shea

Article

Puszynska et al Elife. 2017 Apr 21;6. pii: e23210. doi:

Insert

gentamicin resistance casette replacing rpaA

Use

Cloning vector

Available Since

Jan. 18, 2018

Availability

Academic Institutions and Nonprofits only

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BB1_23_YlGUT1

Plasmid

#117823

Purpose

Encodes glycerol kinase (GUT1) of Y. lipolytica for GoldenMOCS

Depositor

Michael Sauer

Article

Egermeier et al FEMS Microbiol Lett. 2019 Jan 29. pii: 5

Insert

YlGUT1

Expression

Bacterial

Available Since

Feb. 13, 2019

Availability

Academic Institutions and Nonprofits only

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