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pBABE (hygro) mEOS2 hYAP 1-1beta

Plasmid

#124156

Purpose

Protein expression for funtional studies of cell growth promotion

Depositor

Megan Finch-Edmondson, Marius Sudol

Article

Gaffney et al Gene. 2012 Nov 10;509(2):215-22. doi: 10

Insert

mEOS2-YAP1 (YAP1 Human)

Use

Unspecified

Mutation

None (Wild Type)

Available Since

April 5, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1 (-) hYAP 1-1beta

Plasmid

#124140

Purpose

Protein expression for funtional studies of cell growth promotion

Depositor

Megan Finch-Edmondson, Marius Sudol

Article

Gaffney et al Gene. 2012 Nov 10;509(2):215-22. doi: 10

Insert

YAP1 (YAP1 Human)

Use

Unspecified

Mutation

None (Wild Type)

Available Since

April 4, 2019

Availability

Academic Institutions and Nonprofits only

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RF15

Bacterial Strain

#102799

Purpose

BL21(DE3)-based E. coli amino acid auxotrophic host strain used for selective isotope labeling. aspC tyrB trpA trpB glyA serB genes deleted.

Depositor

Robert Gennis, Toshio Iwasaki

Article

Iwasaki et al J Biochem. 2021 Apr 29;169(4):387-394. d

Bacterial Resistance

None

Available Since

Nov. 8, 2017

Availability

Academic Institutions and Nonprofits only
RF16

Bacterial Strain

#102800

Purpose

BL21(DE3)-based E. coli amino acid auxotrophic host strain used for selective isotope labeling. aspC tyrB trpA trpB glyA serB cysE genes deleted.

Depositor

Robert Gennis, Toshio Iwasaki

Article

Iwasaki et al J Biochem. 2021 Apr 29;169(4):387-394. d

Bacterial Resistance

None

Available Since

Nov. 8, 2017

Availability

Academic Institutions and Nonprofits only
RF21

Bacterial Strain

#102803

Purpose

BL21(DE3)-based E. coli amino acid auxotrophic host strain used for selective isotope labeling. aspC tyrB ilvE avtA yfbQ(alaA) yfdZ(alaC) genes deleted. (yfbQ also called alaA; yfdZ also called alaC)

Depositor

Robert Gennis, Toshio Iwasaki

Article

Iwasaki et al J Biochem. 2021 Apr 29;169(4):387-394. d

Bacterial Resistance

None

Available Since

Nov. 8, 2017

Availability

Academic Institutions and Nonprofits only
Wolfe-Lawson ZFN modular assembly kit

Plasmid Kit

#1000000018

Purpose

Contains reagents for engineering zinc finger arrays by the modular assembly method.

Depositor

Nathan Lawson, Scot Wolfe

Available Since

Nov. 9, 2011

Availability

Academic Institutions and Nonprofits only
pAAV-CaMKIIa(0.4)-eOPN3-mScarlet-WPRE

Plasmid

#125712

Purpose

Expresses an optimized mosquito OPN3 rhodopsin in-frame with mScarlet

Depositor

Ofer Yizhar

Article

Mahn et al Neuron. 2021 May 10. pii: S0896-6273(21)

Has Service

AAV Retrograde, AAV1, and AAV5

Insert

eOPN3

Use

AAV

Tags

Rho1D4 and mScarlet

Expression

Mammalian

Mutation

deleted amino acids 331-429

Promoter

short version of αCamKinase II promoter (0.4kb)

Available Since

Feb. 19, 2021

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn1-SIO-eOPN3-mScarlet-WPRE

Plasmid

#125713

Purpose

Cre-dependent vector expressing an optimized mosquito OPN3 rhodopsin in-frame with mScarlet

Depositor

Ofer Yizhar

Article

Mahn et al Neuron. 2021 May 10. pii: S0896-6273(21)

Has Service

AAV1 and AAV5

Insert

eOPN3

Use

AAV and Cre/Lox

Tags

Rho1D4 and mScarlet

Expression

Mammalian

Mutation

deleted amino acids 331-429

Promoter

hSyn1

Available Since

Sept. 20, 2019

Availability

Academic Institutions and Nonprofits only

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pGEX-4T_CIP2A_1-560_R522D

Plasmid

#217923

Purpose

E. coli expression of GST-tagged CIP2A_1-560 with R522D mutation

Depositor

Jukka Westermarck

Insert

CIP2A (CIP2A Human)

Tags

GST

Expression

Bacterial

Mutation

R522D (522 AGA->GAT)

Available Since

May 17, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-CMV-PdCO-mScarlet

Plasmid

#198507

Purpose

Expresses optimized PdCO in frame with mScarlet under control of CMV promotor.

Depositor

Ofer Yizhar

Article

Wietek et al Nat Methods. 2024 May 29. doi: 10.1038/s

Insert

PdCO

Tags

Rho1D4 and mScarlet

Expression

Mammalian

Promoter

CMV

Available Since

July 5, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-CMV-PdCO-EGFP

Plasmid

#198512

Purpose

Expresses optimized PdCO in frame with EGFP under control of CMV promotor.

Depositor

Ofer Yizhar

Article

Wietek et al Nat Methods. 2024 May 29. doi: 10.1038/s

Insert

PdCO

Tags

EGFP and Rho1D4

Expression

Mammalian

Promoter

CMV

Available Since

July 5, 2023

Availability

Academic Institutions and Nonprofits only

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DHIP1

Bacterial Strain

#226851

Purpose

Expresses HIP1 methyltransferase to methylate plasmids for delivery to cyanobacteria

Depositor

David Nielsen

Article

Kamoku et al Biotechnol Prog. 2025 Jan 23:e3518. doi:

Bacterial Resistance

Streptomycin

Species

Escherichia coli

Available Since

Nov. 15, 2024

Availability

Academic Institutions and Nonprofits only
TB205 △ilvA

Bacterial Strain

#230042

Purpose

Fluorescently labelled (mCherry) E. coli, auxotrophic for isoleucine

Depositor

Martin Ackermann, Olga Schubert

Article

Micali et al Proc Natl Acad Sci U S A. 2023 Nov 7;120

Bacterial Resistance

None

Available Since

Jan. 31, 2025

Availability

Academic Institutions and Nonprofits only
TB204 △ilvA

Bacterial Strain

#230041

Purpose

Fluorescently labelled (GFP) E. coli, auxotrophic for isoleucine

Depositor

Martin Ackermann, Olga Schubert

Article

Micali et al Proc Natl Acad Sci U S A. 2023 Nov 7;120

Bacterial Resistance

None

Available Since

Jan. 31, 2025

Availability

Academic Institutions and Nonprofits only
TB205 △proC

Bacterial Strain

#230036

Purpose

Fluorescently labelled (mCherry) E. coli, auxotrophic for proline

Depositor

Martin Ackermann, Olga Schubert

Article

Dal Co et al Nat Ecol Evol. 2020 Mar;4(3):366-375. do

Bacterial Resistance

None

Available Since

Jan. 31, 2025

Availability

Academic Institutions and Nonprofits only
TB204 △proC

Bacterial Strain

#230035

Purpose

Fluorescently labelled (GFP) E. coli, auxotrophic for proline

Depositor

Martin Ackermann, Olga Schubert

Article

Dal Co et al Nat Ecol Evol. 2020 Mar;4(3):366-375. do

Bacterial Resistance

None

Available Since

Jan. 31, 2025

Availability

Academic Institutions and Nonprofits only
TB204 △trpC proB74

Bacterial Strain

#229547

Purpose

Fluorescently labelled (sfGFP) E. coli, auxotrophic for tryptophan, proline overproducer

Depositor

Yolanda Schaerli

Article

Pignon et al ISME J. 2025 Jun 13:wraf122. doi: 10.109

Bacterial Resistance

None

Available Since

June 11, 2025

Availability

Academic Institutions and Nonprofits only
TB204 △metB

Bacterial Strain

#229549

Purpose

Fluorescently labelled (sfGFP) E. coli, auxotrophic for methionine

Depositor

Yolanda Schaerli

Article

Pignon et al ISME J. 2025 Jun 13:wraf122. doi: 10.109

Bacterial Resistance

None

Available Since

June 11, 2025

Availability

Academic Institutions and Nonprofits only
TB204 △hisD proB74

Bacterial Strain

#229550

Purpose

Fluorescently labelled (sfGFP) E. coli, auxotrophic for histidine, proline overproducer

Depositor

Yolanda Schaerli

Article

Pignon et al ISME J. 2025 Jun 13:wraf122. doi: 10.109

Bacterial Resistance

None

Available Since

June 11, 2025

Availability

Academic Institutions and Nonprofits only
TB204 △metB proB74

Bacterial Strain

#229551

Purpose

Fluorescently labelled (sfGFP) E. coli, auxotrophic for methionine, proline overproducer

Depositor

Yolanda Schaerli

Article

Pignon et al ISME J. 2025 Jun 13:wraf122. doi: 10.109

Bacterial Resistance

None

Available Since

June 11, 2025

Availability

Academic Institutions and Nonprofits only