We narrowed to 74,254 results for: prot
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Plasmid Kit#1000000160PurposeThe Seeger Lab protocol to select synthetic nanobodies – called sybodies – against (membrane) protein targets. This collection contains all plasmids necessary for the sybody generation.DepositorAvailable SinceApril 15, 2020AvailabilityAcademic Institutions and Nonprofits only
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pLCKO2::TKOv3
Pooled Library#125517PurposeHuman CRISPR lentiviral library containing 70,948 guides (4-5 guides/gene) targeting 18,053 human protein-coding genes, in a non-Cas9 containing backboneDepositorExpressionMammalianSpeciesHomo sapiensUseCRISPR and LentiviralAvailable SinceAug. 1, 2019AvailabilityAcademic Institutions and Nonprofits only -
plIG-123_HA-GD2-28z_CAR_TF_Library
Pooled Library#207478PurposeHA-GD2-28z CAR in combination with 100 transcription factors (and related proteins) and 2 controls (GFP/RFP). Library can be used to generate HDR templates for modular pooled knockin (ModPoKI) into thDepositorSpeciesHomo sapiensUseCRISPRAvailable SinceJan. 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
plIG-128_HA-GD2-28z_CAR_TFxTF_Library
Pooled Library#207480PurposeHA-GD2-28z CAR in combination with ~10,000 transcription factor combinations (and related proteins) and controls (GFP/RFP combinations). Library can be used to generate HDR templates for modular pooleDepositorUseCRISPRAvailable SinceJan. 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
plIG-107_NY-ESO-1_TCR_TF_Library
Pooled Library#207475PurposeNY-ESO-1 TCR in combination with 100 transcription factors (and related proteins) and 2 controls (GFP/RFP). Library can be used to generate HDR templates for modular pooled knockin (ModPoKI) into theDepositorSpeciesHomo sapiensUseCRISPRAvailable SinceJan. 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
plIG-110_CD19-BBz_CAR_TF_Library
Pooled Library#207477PurposeCD19-BBz CAR in combination with 100 transcription factors (and related proteins) and 2 controls (GFP/RFP). Library can be used to generate HDR templates for modular pooled knockin (ModPoKI) into theDepositorSpeciesHomo sapiensUseCRISPRAvailable SinceJan. 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
plIG-132_CD19-28z_CAR_TF_and_SR_Library
Pooled Library#207481PurposeCD19-28z CAR in combination with 129 surface receptors, 100 transcription factors (and related proteins) and 2 controls (GFP/RFP). Library can be used to generate HDR templates for modular pooled knocDepositorSpeciesHomo sapiensUseCRISPRAvailable SinceJan. 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
MoBiFC
Plasmid Kit#1000000222PurposeThe modular bimolecular fluorescence complementation (MoBiFC) toolkit allows for the analysis of protein-protein interactions in plants.DepositorAvailable SinceJuly 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
Yeast Secrete and Detect
Plasmid Kit#1000000166PurposeYeast Golden Gate cloning for protein secretion in Saccharomyces cerevisiae and Pichia pastoris. Methanol utilization (MUT) promoters, signal peptides, C-terminal tags, episomal and integrative.DepositorAvailable SinceJan. 28, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-EGFP-DmEDC3_441-680_D
Plasmid#146127PurposeInsect Expression of DmEDC3_441-680DepositorInsertDmEDC3_441-680 (Edc3 Fly)
ExpressionInsectMutation3 mutations N162T, K221E and D418G compared to th…Available SinceFeb. 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1 EWS-myc-HIS
Plasmid#46386Purposeexpresses myc, HIS tagged EWSR1 in mammalian cellsDepositorAvailable SinceJuly 29, 2013AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1 His-GFP-EWSR1-myc-His
Plasmid#46385Purposeexpresses EGFP tagged EWSR1 in mammalian cellsDepositorAvailable SinceSept. 5, 2013AvailabilityAcademic Institutions and Nonprofits only -
pHAGE-CMV-Flag-GFP_IDG-K
Plasmid#135275PurposeGateway destination clone of GFP (as control) tagged with N-terminal Flag for generating protein-protein networks using fusion tag affinity-based proteomicsDepositorInsertFlag-GFP
UseLentiviral; Gateway destinationTagsFlagExpressionMammalianPromoterCMVAvailable SinceDec. 23, 2019AvailabilityAcademic Institutions and Nonprofits only -
pHAGE-CMV-Flag-hcRed_IDG-K
Plasmid#135274PurposeGateway destination clone of hcRed (as control) tagged with N-terminal Flag for generating protein-protein networks using fusion tag affinity-based proteomicsDepositorInsertFlag-hcRed
UseLentiviral; Gateway destinationTagsFlagExpressionMammalianPromoterCMVAvailable SinceDec. 20, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCS6-mPSD95a-TSa-HA
Plasmid#42235PurposeTimeSTAMP method for tagging and visualization of newly synthesized proteins. TimeSTAMPa cassette used for for low abundance proteins or proteins with slow ongoing synthesis ratesDepositorInsertPSD95
TagsTSa-HAExpressionMammalianAvailable SinceMarch 13, 2013AvailabilityAcademic Institutions and Nonprofits only -
pCS6-mPSD95a-TSt-HA
Plasmid#42237PurposeTimeSTAMP method for tagging and visualization of newly synthesized proteins. TimeSTAMPt cassette used for highly expressed proteins or proteins with faster ongoing synthesis ratesDepositorInsertPSD95
TagsTSt-HAExpressionMammalianAvailable SinceMarch 13, 2013AvailabilityAcademic Institutions and Nonprofits only -
pHAGE-CMV-Flag-TLK2_IDG-K
Plasmid#135278PurposeGateway destination clone of TLK2 (human) tagged with N-terminal Flag for generating protein-protein networks using fusion tag affinity-based proteomicsDepositorInsertFlag-TLK2 (TLK2 Human)
UseLentiviral; Gateway destinationTagsFlagExpressionMammalianPromoterCMVAvailable SinceDec. 23, 2019AvailabilityAcademic Institutions and Nonprofits only -
pHAGE-CMV-Flag-PKMYT1_IDG-K
Plasmid#135277PurposeGateway destination clone of PKMYT1 (human) tagged with N-terminal Flag for generating protein-protein networks using fusion tag affinity-based proteomicsDepositorInsertFlag-PKMYT1 (PKMYT1 Human)
UseLentiviral; Gateway destinationTagsFlagExpressionMammalianPromoterCMVAvailable SinceDec. 20, 2019AvailabilityAcademic Institutions and Nonprofits only -
pINTO-N-SA
Plasmid#61723PurposeInducible expression of proteins fused to an N-terminal monomeric streptavidin tag (mSA)DepositorTypeEmpty backboneTagsmSAExpressionMammalianPromoterT-REx (CMV + 2xTetO2)Available SinceJan. 30, 2015AvailabilityAcademic Institutions and Nonprofits only -
pET28-OmpT
Plasmid#68862Purposeconstruct for expression of OmpT protease in the pET28a plasmid using the restriction sites NcoI and EcoRI.DepositorAvailable SinceOct. 9, 2015AvailabilityAcademic Institutions and Nonprofits only