We narrowed to 3,408 results for: guide rna expression plasmid

1. pSL1119 (pDonor)

   Plasmid

   #160732

   Purpose

   Mini-transposon donor plasmid for V. cholerae CAST system. pUC19 vector backbone.

   Depositor

   Samuel H. Sternberg

   Article

   Vo et al Nat Biotechnol. 2020 Nov 23. pii: 10.103

   Insert

   VchCAST donor DNA (Mini-Tn)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   N/A

   Available since

   Dec. 1, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pICSL01009::AtU6p

   Plasmid

   #46968

   Purpose

   Encodes the Arabidopsis U6 promoter in a level 0 vector (Weber et al. PLoS One 6:e16765, 2011). It is used to place an sgRNA uder the Arabidopsis U6 promoter.

   Depositor

   Sophien Kamoun

   Article

   Nekrasov et al Nat Biotechnol. 2013 Aug 8;31(8):691-3.

   Insert

   AtU6p
   

   Use

   CRISPR

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Available since

   Aug. 15, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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3. pX459-sg-tdTomato90/816

   Plasmid

   #179918

   Purpose

   Double cutting Cas9 plasmid with puromycin resistance and a single guide RNA targeting tdTomato fluorescent protein sites 90 and 816.

   Depositor

   Indeever Madireddy, Johan Sosa

   Article

   Madireddy et al MicroPubl Biol. 2022 Jun 16;2022. doi: 1

   Insert

   tdTomato sgRNA
   

   Use

   CRISPR

   Tags

   2A-Puro and 3XFLAG

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   April 4, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pX459-sg-tdTomato166/892

   Plasmid

   #179919

   Purpose

   Double cutting Cas9 plasmid with puromycin resistance and a single guide RNA targeting tdTomato fluorescent protein sites 166 and 892.

   Depositor

   Indeever Madireddy, Johan Sosa

   Article

   Madireddy et al MicroPubl Biol. 2022 Jun 16;2022. doi: 1

   Insert

   tdTomato sgRNA
   

   Use

   CRISPR

   Tags

   2A-Puro and 3XFLAG

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   April 4, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pMB60

   Plasmid

   #47941

   Purpose

   Can be used to generate synthetic guide in vitro from T7 promotor

   Depositor

   Mike Boxem

   Article

   Waaijers et al Genetics. 2013 Aug 26.

   Insert

   synthetic guide RNA
   

   Use

   CRISPR

   Tags

   Expression

   Worm

   Mutation

   Promoter

   Available since

   Oct. 4, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCAS9i_TRP

   Plasmid

   #141253

   Purpose

   Galactose-inducible expression of Cas9 for addressing one target; Contains guide RNA expression cassette with stuffer and KpnI-Pme1 restriction sites.

   Depositor

   Adam Bertl

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   GAL1 (Cas9), pSNR52 (gRNA)

   Available since

   Aug. 19, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCAS9id_TRP

   Plasmid

   #141254

   Purpose

   Galactose-inducible expression of Cas9 for addressing two targets; Contains two guide RNA expression cassettes with stuffer and Kpn restriction sites.

   Depositor

   Adam Bertl

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   GAL1 (Cas9), pSNR52 (gRNA)

   Available since

   Aug. 19, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. TMPRSS2 g2

   Plasmid

   #153016

   Purpose

   A guide RNA targeting TMPRSS2 in a lentiviral plasmid co-expressing mCherry

   Depositor

   Jason Sheltzer

   Insert

   TMPRSS2 gRNA
   

   Use

   CRISPR and Lentiviral

   Tags

   mCherry

   Expression

   Mutation

   Promoter

   Available since

   June 2, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. TMPRSS2 g3

   Plasmid

   #153017

   Purpose

   A guide RNA targeting TMPRSS2 in a lentiviral plasmid co-expressing mCherry

   Depositor

   Jason Sheltzer

   Insert

   TMPRSS2 gRNA
   

   Use

   CRISPR and Lentiviral

   Tags

   mCherry

   Expression

   Mutation

   Promoter

   Available since

   June 2, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. pSc1-puro

    Plasmid

    #80438

    Purpose

    Expresses eGFP along with an U6 promoter driven gRNA which cleaves the plasmid in-cell, and also confers resistance to puromycin

    Depositor

    Ervin Welker

    Article

    Talas et al DNA Res. 2017 Jun 30. doi: 10.1093/dnare

    Inserts

    sp Cas9 gRNA
    eGFP
    Puromycin resistance
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV, U6, and hPGK

    Available since

    Sept. 12, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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