We narrowed to 72,784 results for: des.1

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pet 3d alpha 1 beta 2 CP

Plasmid

#13450

Depositor

John Cooper

Article

Wear et al Curr Biol. 2003 Sep 2. 13(17):1531-7.

Insert

capping protein alpha 1 beta 2 (mouse) (Capza1 Mouse)

Expression

Bacterial

Mutation

none

Available Since

Dec. 15, 2006

Availability

Academic Institutions and Nonprofits only
p416GAL1-Zipp.-L-IFP-F[1] (Yeast)

Plasmid

#52895

Purpose

Plasmid+insert encoding GCN4-leucine zipper fused to IFP-F[1] by the linker (GGGGS) X 2. Zipper-L-IFP-F[1] (XbaI/XhoI). Zipper is (XbaI /ClaI). Plasmid confers ampicillin resistance to DH5α.

Depositor

Stephen Michnick

Article

Tchekanda et al Nat Methods. 2014 Jun;11(6):641-4. doi:

Insert

Zipp.-L-IFP-F[1]

Tags

L-IFP-F[1]

Expression

Yeast

Promoter

GAL1

Available Since

July 22, 2014

Availability

Academic Institutions and Nonprofits only
pLenti X2 Puro/pTER shLUC (w589-1)

Plasmid

#21472

Purpose

3rd gen lentiviral Luciferase shRNA (H1/TO promoter), 3’LTR insertion, Puro

Depositor

Eric Campeau

Article

Campeau et al PLoS One. 2009 Aug 6;4(8):e6529.

Insert

Firefly luciferase shRNA

Use

Lentiviral and RNAi

Available Since

July 22, 2009

Availability

Academic Institutions and Nonprofits only

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pLenti X2 Neo/pTER shLUC (w181-1)

Plasmid

#17483

Purpose

3rd gen lentiviral Luciferase shRNA (H1/TO promoter), 3’LTR insertion, Neo

Depositor

Eric Campeau, Paul Kaufman

Article

Campeau et al PLoS One. 2009 Aug 6;4(8):e6529.

Insert

Firefly Luciferase shRNA

Use

Lentiviral and RNAi

Available Since

Aug. 12, 2009

Availability

Academic Institutions and Nonprofits only

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pLenti X2 Blast/pTER shLUC (w618-1)

Plasmid

#20962

Purpose

3rd gen lentiviral Luciferase shRNA (H1/TO promoter), 3’LTR insertion, Blast

Depositor

Eric Campeau

Article

Campeau et al PLoS One. 2009 Aug 6;4(8):e6529.

Insert

Firefly luciferase shRNA

Use

Lentiviral and RNAi

Available Since

July 22, 2009

Availability

Academic Institutions and Nonprofits only

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pLenti X2 Zeo/pTER shLUC (w648-1)

Plasmid

#21561

Purpose

3rd gen lentiviral Luciferase shRNA (H1/TO promoter), 3’LTR insertion, Zeo

Depositor

Eric Campeau

Article

Campeau et al PLoS One. 2009 Aug 6;4(8):e6529.

Insert

Firefly luciferase shRNA

Use

Lentiviral and RNAi

Available Since

July 22, 2009

Availability

Academic Institutions and Nonprofits only

Enlarge
pSG5-FLAG-CaMKKbeta rat 1-460 K193A

Plasmid

#33326

Depositor

Anthony Means

Article

Green et al Cell Signal. 2011 Dec;23(12):2005-12. Ep

Insert

Calcium/Calmodulin dependent protein kinase kinase beta (Camkk2 Rat)

Tags

FLAG

Expression

Mammalian

Mutation

truncated at 460, K193A (S3V, G96R, and P108L als…

Promoter

SV40

Available Since

April 17, 2012

Availability

Academic Institutions and Nonprofits only
pSG5-FLAG-CaMKKbeta rat 1-460 D311A

Plasmid

#33325

Depositor

Anthony Means

Article

Green et al Cell Signal. 2011 Dec;23(12):2005-12. Ep

Insert

Calcium/Calmodulin dependent protein kinase kinase beta (Camkk2 Rat)

Tags

FLAG

Expression

Mammalian

Mutation

truncated at 460, D311A (S3V, G96R, and P108L als…

Promoter

SV40

Available Since

April 17, 2012

Availability

Academic Institutions and Nonprofits only
p416GAL1-Ssk1t-L-IFP-F[1] (Yeast)

Plasmid

#52897

Purpose

Ssk1t replaces the zipper and is fused to the IFP-F[1] through the linker (GGGGS) X 2. Plasmid confers ampicillin resistance to DH5α.

Depositor

Stephen Michnick

Article

Tchekanda et al Nat Methods. 2014 Jun;11(6):641-4. doi:

Insert

Ssk1t-L-IFP-F[1]

Tags

L-IFP-F[1]

Expression

Yeast

Promoter

GAL1

Available Since

June 5, 2014

Availability

Academic Institutions and Nonprofits only
hFLN29-N/pCMV-14-FLAG (1-I210)

Plasmid

#73588

Purpose

Express N-terminal part (1-I210) of hTRAFD1 (FLN29) in mammalian cells

Depositor

Paul Odgren

Article

Witwicka et al PLoS One. 2015 May 19;10(5):e0127537. do

Insert

TRAFD1 (TRAFD1 Human)

Tags

3xFlag

Expression

Mammalian

Available Since

March 14, 2016

Availability

Academic Institutions and Nonprofits only

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p416GAL1-Skn7t-L-IFP-F[1] (Yeast)

Plasmid

#52896

Purpose

Skn7t replaces the zipper and is fused to the IFP-F[1] through the linker (GGGGS) X 2. Plasmid confers ampicillin resistance to DH5α.

Depositor

Stephen Michnick

Article

Tchekanda et al Nat Methods. 2014 Jun;11(6):641-4. doi:

Insert

Skn7t-L-IFP-F[1]

Tags

L-IFP-F[1]

Expression

Yeast

Promoter

GAL1

Available Since

June 12, 2014

Availability

Academic Institutions and Nonprofits only

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pGL2 enhancer- F3 GATA Sp-1 mut

Plasmid

#22431

Depositor

William Sessa

Article

Zhang et al J Biol Chem. 1995 Jun 23. 270(25):15320-

Insert

F3 GATA Sp-1 mut (NOS3 Human)

Use

Luciferase

Tags

luciferase reporter gene

Expression

Mammalian

Available Since

Feb. 17, 2010

Availability

Academic Institutions and Nonprofits only
p5068 pGEX-6P-1 Brd4 full-length

Plasmid

#14447

Depositor

Peter Howley

Article

You et al Cell. 2004 Apr 30. 117(3):349-60.

Insert

Brd4 (BRD4 Human)

Tags

GST

Expression

Bacterial

Available Since

March 2, 2007

Availability

Academic Institutions and Nonprofits only

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pet151D-TOPO ALIX Bro1-V (1-702)

Plasmid

#80642

Purpose

Residues 1-702 of ALIX; Bacterial Expression Vector; TEV cleavable N-term His-tag; Sundquist Lab Internal ID: WISP10-648

Depositor

Wesley Sundquist

Article

McCullough et al Proc Natl Acad Sci U S A. 2008 Jun 3;105

Insert

ALIX Bro1-V (PDCD6IP Human)

Tags

His Tag

Expression

Bacterial

Mutation

Residues 1-702

Available Since

Sept. 19, 2016

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-TAT-1-101-FLAG basic mutant

Plasmid

#35985

Depositor

Susana Valente

Article

Mousseau et al Cell Host Microbe. 2012 Jul 19;12(1):97-

Insert

transactivating protein (tat )

Tags

Flag

Expression

Mammalian

Mutation

tat from HIV1, 101 aa; R to A mutations at aa 56,…

Available Since

Sept. 6, 2012

Availability

Academic Institutions and Nonprofits only

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p6873 pHAGE-N-V5-MAML1 (1-990)

Plasmid

#37049

Depositor

Peter Howley

Article

Tan et al Proc Natl Acad Sci U S A. 2012 Apr 30.

Insert

MAML1(1-990) (MAML1 Human)

Tags

V5

Expression

Mammalian

Promoter

CMVt

Available Since

July 20, 2012

Availability

Academic Institutions and Nonprofits only

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His-ZZ-TEV-SNAPf-BICD2N(1-400)

Plasmid

#111857

Purpose

N-terminal SNAPf tag on BICD2N (1-400) for expression in Sf9 cells. 8xHis-ZZ-SNAPf N-terminal tag, TEV site to cleave 8xHis-ZZ.

Depositor

Andrew Carter

Article

Urnavicius et al Nature. 2018 Feb 7;554(7691):202-206. do

Insert

BICD2N(1-400)

Tags

His-tag, SNAPf-tag, TEV site, and ZZ

Expression

Insect

Mutation

Optimised for Sf9 expression

Available Since

July 30, 2018

Availability

Academic Institutions and Nonprofits only

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pET51b(+)-NADPH-LUCID-1 (C50 5.1 nM)

Plasmid

#117908

Purpose

Plasmid encoding the bacterial expression of the BRET semi-synthetic biosensor measuring the NADPH concentration with a C50 of 5.1 nM

Depositor

Kai Johnsson

Article

Yu et al Science. 2018 Sep 14;361(6407):1122-1126

Insert

SNAP_Pro30_[cpNLuc23_24]eDHFR

Tags

His-tag and Strep-tag

Expression

Bacterial

Promoter

T7

Available Since

Oct. 25, 2018

Availability

Academic Institutions and Nonprofits only

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pBEST-p15a-PLtetO-1-UTR1-LacI-T500

Plasmid

#45784

Depositor

Richard Murray, Vincent Noireaux

Article

Sun et al ACS Synth Biol. 2014 Jun 20;3(6):387-97.

Insert

Lac repressor

Use

Synthetic Biology

Expression

Bacterial

Promoter

PLtetO-1

Available Since

July 16, 2013

Availability

Academic Institutions and Nonprofits only

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pOPINK-OTUB1 (full-length, aa 1-271)

Plasmid

#61420

Purpose

Expresses human OTUB1 (full-length) in E. coli.

Depositor

David Komander

Article

Mevissen et al Cell. 2013 Jul 3;154(1):169-84. doi: 10.

Insert

OTUB1 (Ubiquitin thioesterase OTUB1) (OTUB1 Human)

Tags

His6-GST-3C

Expression

Bacterial

Promoter

T7

Available Since

March 12, 2015

Availability

Academic Institutions and Nonprofits only

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