169,099 results

1. pTET2-RlucCP

   Plasmid

   #198352

   Purpose

   Part of Firefly reporter system, encodes Renilla luciferase

   Depositor

   Eugene Yeo

   Article

   Luo et al Nat Struct Mol Biol. 2020 Oct;27(10):989

   Insert

   Renilla Luciferase
   

   Use

   Luciferase

   Expression

   Mammalian

   Promoter

   pTET2

   Available Since

   April 20, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. TFORF0194

   Plasmid

   #142363

   Purpose

   Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

   Depositor

   Feng Zhang

   Article

   Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

   Insert

   RFX6 (RFX6 Human)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   EF1a

   Available Since

   June 20, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. mCerulean3-Lifeact-7

   Plasmid

   #54721

   Purpose

   Localization: Actin, Excitation: 433, Emission: 475

   Depositor

   Michael Davidson

   Article

   Markwardt et al PLoS One. 2011 . 6(3):e17896.

   Insert

   Lifeact-mCerulean3
   

   Expression

   Mammalian

   Available Since

   July 25, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCMV4 p50

   Plasmid

   #21965

   Depositor

   Warner Greene

   Article

   Ballard et al Proc Natl Acad Sci U S A. 1992 Mar 1. 89

   Insert

   NF-kB p50 subunit (NFKB1 Human)
   

   Expression

   Mammalian

   Available Since

   Sept. 17, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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5. MMP11-bio-His

   Plasmid

   #53408

   Purpose

   Expresses full-length Stromelysin-3 precursor ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.

   Depositor

   Gavin Wright

   Article

   Sun et al Mol Cell Proteomics. 2015 Feb 23. pii: m

   Insert

   MMP11 (MMP11 Human)
   

   Tags

   His tag, enzymatic biotinylation sequence, and ra…

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Feb. 27, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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6. aCat TS WT (1-697 mTFP1-TSmod-Venus 698-907)

   Plasmid

   #101299

   Purpose

   FRET-based tension sensor (TS) technology to develop a probe for molecular-level tension across alphaE-catenin.

   Depositor

   Alpha Yap

   Article

   Acharya et al Cell Rep. 2017 Mar 21;18(12):2854-2867.

   Insert

   alpha-Catenin
   

   Expression

   Mammalian

   Mutation

   Contains amino acids 1-698 and 698-906 of alpha-C…

   Available Since

   June 11, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. AAV-CAG-jGCaMP8s-WPRE (AAV1)

   Viral Prep

   #179256-AAV1

   Purpose

   Ready-to-use AAV1 particles produced from AAV-CAG-jGCaMP8s-WPRE (#179256). In addition to the viral particles, you will also receive purified AAV-CAG-jGCaMP8s-WPRE plasmid DNA. CAG-driven expression of calcium sensor GCaMP8s. These AAV preparations are suitable purity for injection into animals.

   Depositor

   Loren Looger

   Promoter

   CAG

   Available Since

   Jan. 24, 2024

   Availability

   Academic Institutions and Nonprofits only

8. pSAB35

   Plasmid

   #175787

   Purpose

   Expresses Legionella pneumophila SidK (1-278) with a cleavable N-terminal His tag (TEV site) and C-terminal 3xFLAG tag

   Depositor

   John Rubinstein

   Article

   Abbas et al Science. 2020 Mar 13;367(6483):1240-1246

   Insert

   SidK (1-278)
   

   Tags

   3xFLAG and 6xHis TEV

   Expression

   Bacterial

   Available Since

   Dec. 21, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pSLIK_Hyg_mTurquoise2_Atg4BC74A

   Plasmid

   #161733

   Purpose

   Doxycycline-inducible expression of Atg4B(C74A) fused with mTurquoise2 to inhibit autophagy

   Depositor

   Alec Kimmelman

   Article

   Yamamoto et al Nature. 2020 May;581(7806):100-105. doi:

   Insert

   Atg4B(C74A) (Atg4b Mouse) (Atg4b Mouse)
   

   Use

   Lentiviral; Destinatioin vector for gateway cloni…

   Tags

   mTurquoise2

   Expression

   Mammalian

   Mutation

   220-222 TGC (Cys) is changed to GCA (Ala)

   Available Since

   Nov. 30, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. pMpGWB309

    Plasmid

    #68637

    Purpose

    Gateway binary vector designed for transgenic research with Marchantia polymorpha as well as other plants

    Depositor

    Takayuki Kohchi

    Article

    Ishizaki et al PLoS One. 2015 Sep 25;10(9):e0138876. do

    Type

    Empty backbone

    Tags

    3xFLAG

    Expression

    Plant

    Available Since

    June 24, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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11. Ac5-STABLE1-neo

    Plasmid

    #32425

    Depositor

    Rosa Barrio, James Sutherland

    Article

    Gonzalez et al Sci Rep. 2011;1:75. doi: 10.1038/srep000

    Insert

    GFP-T2A-neo
    

    Expression

    Insect

    Promoter

    Actin5c

    Available Since

    Sept. 29, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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12. pGWB405

    Plasmid

    #74799

    Purpose

    Gateway cloning compatible binary vector for C-terminal fusion with sGFP (CaMV35S promoter).

    Depositor

    Tsuyoshi Nakagawa

    Article

    Nakagawa et al Biosci Biotechnol Biochem. 2007 Aug;71(8

    Type

    Empty backbone

    Expression

    Plant

    Promoter

    CaMV35S

    Available Since

    July 18, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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13. pcDNA3.1-ERBB2-V5/HIS

    Plasmid

    #201103

    Purpose

    expression of human ERBB2 receptor tyrosine kinase in mammalian cells

    Depositor

    Pavel Krejčí

    Article

    Gudernova et al Elife. 2017 Feb 15;6. pii: e21536. doi:

    Insert

    human ERBB2 receptor tyrosine kinase, full length, wildtype (ERBB2 Human)
    

    Tags

    V5/His

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    May 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. pcDNA5/FRT/TO-GAlphaQ-RLuc8

    Plasmid

    #140982

    Purpose

    Encodes a G alpha subunit (GNAQ) containing RLuc8 as an optimal component of a BRET2 biosensor for studying heterotrimeric G protein dissociation

    Depositor

    Bryan Roth

    Article

    Olsen et al Nat Chem Biol. 2020 May 4. pii: 10.1038/

    Insert

    GAlphaQ-RLuc8 (GNAQ Human)
    

    Use

    Luciferase

    Expression

    Mammalian

    Mutation

    RLuc8 and flanking SGGGGS linkers have been inser…

    Promoter

    CMV

    Available Since

    June 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. pDragon-Ctgf

    Plasmid

    #155015

    Purpose

    For Flp-mediated cassette exchange. Expresses tdTomato in the presence of (r)tTA, switching to connective tissue growth factor in the presence of Cre and (r)tTA activity.

    Depositor

    Alberto Rosello-Diez

    Article

    Ahmadzadeh et al Development. 2020 May 28;147(10). pii: d

    Insert

    Ccn2 (Ccn2 Mouse)
    

    Use

    Mouse Targeting

    Mutation

    M24T (please see depositor comments)

    Available Since

    Oct. 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. mCherry-PNKP

    Plasmid

    #215137

    Purpose

    Expresses mCherry-PNKP in mammalian cells from a retroviral vector.

    Depositor

    Michele Pagano

    Article

    Rona et al Mol Cell. 2024 Feb 29:S1097-2765(24)0012

    Insert

    PNKP (PNKP Human)
    

    Use

    Retroviral

    Tags

    mCherry

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    April 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. pEF1-Pls3-sfGFP

    Plasmid

    #205750

    Purpose

    Expresses sfGFP under IPTG inducible promoter Pls3 in high copy pTRKH2 gram-positive shuttle vector. Used in Lactobacillus gasseri. Medium/High strength.

    Depositor

    Jerome Bonnet

    Article

    Fristot et al ACS Synth Biol. 2024 Mar 15;13(3):951-95

    Type

    Empty backbone

    Use

    Shuttle vector gram+ gram-

    Expression

    Bacterial

    Promoter

    Pls3 (Phyperspank mutant, IPTG inducible)

    Available Since

    July 14, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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18. mCherry-hALIX

    Plasmid

    #21504

    Depositor

    James Hurley

    Article

    Lee et al Science. 2008 Oct 24. 322(5901):576-80.

    Insert

    ALIX (PDCD6IP Human)
    

    Tags

    Cherry

    Expression

    Bacterial

    Mutation

    none

    Available Since

    Sept. 9, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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19. MSP1830

    Plasmid

    #70708

    Purpose

    Human expression plasmid for SaCas9 KKH variant: CAG-humanSaCas9(E782K/N968K/R1015H)-NLS-3xFLAG

    Depositor

    Keith Joung

    Article

    Kleinstiver et al Nat Biotechnol. 2015 Nov 2. doi: 10.1038

    Insert

    mammalian codon-optimized KKH variant Staphylococcus aureus Cas9
    

    Use

    CRISPR

    Tags

    NLS-3xFLAG

    Expression

    Mammalian

    Mutation

    E782K, N968K and R1015H in SaCas9

    Promoter

    CAG

    Available Since

    Nov. 6, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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20. murine TCR OTII-2A.pMIG II

    Plasmid

    #52112

    Purpose

    retroviral expression of both TCRalpha and TCRbeta using a single vector

    Depositor

    Dario Vignali

    Article

    Holst et al Nat Protoc. 2006;1(1):406-17.

    Inserts

    T cell receptor alpha chain (Tcra Mouse)
    T cell receptor beta chain (Tcrb Mouse)
    

    Use

    Retroviral

    Tags

    P2A (from porcine teschovirus-1)

    Expression

    Mammalian

    Available Since

    April 9, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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