167,475 results

1. pGGG-AH-VRT-A2

   Plasmid

   #163703

   Purpose

   Wheat transformation vector pGoldenGreenGate (pGGG) with OsActinP:: hygromycin (hpt) selection and the full native Triticum polonicum VRT-A2 gene (native prom::genomic seq::3'UTR)

   Depositor

   Cristobal Uauy

   Article

   Adamski et al Plant Cell. 2021 Aug 13;33(7):2296-2319.

   Inserts

   Os Actin promoter :: Hygromycin resistance gene (hpt) containing CAT1 intron :: NosTerminator
   Triticum polonicum VRT-A2 genomic sequence (Native promoter:: genomic sequence::3'UTR) + Nos Terminator
   

   Expression

   Plant

   Promoter

   Rice - Os Actin promoter and Triticum polonicum V…

   Available Since

   Jan. 21, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pMAL-c5X-GLP-1

   Plasmid

   #66997

   Purpose

   Expresses malE-GLP-1 in E. coli

   Depositor

   Ashutosh Chilkoti

   Article

   Tang et al Nat Mater. 2016 Jan 4. doi: 10.1038/nmat

   Insert

   malE-GLP-1
   

   Tags

   His6 and malE-Factor Xa

   Expression

   Bacterial

   Promoter

   tac

   Available Since

   Jan. 5, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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3. pLJM5-WT PHGDH

   Plasmid

   #83901

   Purpose

   stable overexpression

   Depositor

   David Sabatini

   Article

   Pacold et al Nat Chem Biol. 2016 Jun;12(6):452-8. doi

   Insert

   Phosphoglycerate dehydrogenase (PHGDH Human)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Oct. 20, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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4. pGFPGUSPlus

   Plasmid

   #64401

   Purpose

   binary plant vector expressing GFP and GUS, both driven by a 35S promoter

   Depositor

   Claudia Vickers

   Article

   Vickers et al Biotechnol Lett. 2007 Nov;29(11):1793-6.

   Inserts

   GFP
   GUSPlus
   

   Use

   Binary vector for plant transformation

   Tags

   His

   Expression

   Plant

   Mutation

   S65T

   Promoter

   35S

   Available Since

   May 19, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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5. IRES-mCherry

   Plasmid

   #80139

   Purpose

   empty retroviral vector with IRES-mCherry

   Depositor

   Ellen Rothenberg

   Article

   Kueh et al Science. 2013 Aug 9;341(6146):670-3. doi

   Type

   Empty backbone

   Use

   Retroviral

   Tags

   IRES-mCherry

   Expression

   Mammalian

   Available Since

   July 19, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. pRU294

   Plasmid

   #167689

   Purpose

   Final destination vector for single Gateway LR reaction

   Depositor

   Niko Geldner

   Article

   Ursache et al Plant Methods. 2021 Oct 30;17(1):111. do

   Insert

   pEC1.2::Cas9i
   

   Use

   CRISPR

   Available Since

   July 20, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pRG203MX

   Plasmid

   #64521

   Purpose

   Integrative E. coli/S. cerevisiae shuttle vector

   Depositor

   Joerg Stelling

   Article

   Gnugge et al Yeast. 2015 Dec 9. doi: 10.1002/yea.3144

   Type

   Empty backbone

   Expression

   Yeast

   Available Since

   Aug. 28, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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8. pAAV-hDlx-GiDREADD-dTomato-Fishell-5 (AAV9)

   Viral Prep

   #83896-AAV9

   Purpose

   Ready-to-use AAV9 particles produced from pAAV-hDlx-GiDREADD-dTomato-Fishell-5 (#83896). In addition to the viral particles, you will also receive purified pAAV-hDlx-GiDREADD-dTomato-Fishell-5 plasmid DNA. hDlx-driven expression of Gi-DREADD and nuclear localized dTomato (physically separate). These AAV preparations are suitable purity for injection into animals.

   Depositor

   Gordon Fishell

   Article

   Dimidschstein et al Nat Neurosci. 2016 Oct 31. doi: 10.1038/

   Promoter

   hDlx

   Tags

   dTomato (physically separate, not a fusion protein)

   Available Since

   Nov. 9, 2020

   Availability

   Academic Institutions and Nonprofits only

9. pSOUP

   Plasmid

   #165419

   Purpose

   The pSOUP helper plasmid needed for replication of all pGreen (Hellens et al., 2000) based vectors in Agrobacterium

   Depositor

   John Innes Centre - Wheat Transformation and Genome Editing, Wendy Harwood

   Article

   Hayta et al Curr Protoc. 2021 Mar;1(3):e58. doi: 10.

   Insert

   Rep A
   

   Use

   Helper plasmid required for replication for all p…

   Available Since

   April 26, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pAK_DR30_CasRx_GFP

    Plasmid

    #134843

    Purpose

    All in one overexpression of CasRx and guideRNA

    Depositor

    Michael Yaffe

    Insert

    RfxCas13d
    

    Use

    CRISPR

    Tags

    T2A EGFP

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    Jan. 10, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. EasyClone-MarkerFree Vector Set

    Plasmid Kit

    #1000000098

    Purpose

    Yeast (S. cerevisiae) vector toolkit for marker-less integration of genes into 11 predetermined EasyClone Chromosomal loci via CRISPR-Cas9

    Depositor

    Irina Borodina

    Available Since

    Dec. 19, 2016

    Availability

    Academic Institutions and Nonprofits only

12. pAAV-EF1a-fDIO-Cre (AAV9)

    Viral Prep

    #121675-AAV9

    Purpose

    Ready-to-use AAV9 particles produced from pAAV-EF1a-fDIO-Cre (#121675). In addition to the viral particles, you will also receive purified pAAV-EF1a-fDIO-Cre plasmid DNA. EF1a-driven, Flp recombinase dependent expression of Cre recombinase. These AAV preparations are suitable purity for injection into animals.

    Depositor

    Alexander Nectow, Esteban Engel

    Article

    Schneeberger et al Cell. 2019 Jun 24. pii: S0092-8674(19)30

    Promoter

    EF1a

    Available Since

    June 4, 2019

    Availability

    Academic Institutions and Nonprofits only

13. pcDN3.1(+)-CaNARi

    Plasmid

    #64848

    Purpose

    FRET-based calcineurin (CaN) activation biosensor.

    Depositor

    Jin Zhang

    Article

    Mehta et al eLife. 2014 Jul 23;3:e03765. doi: 10.755

    Insert

    CaNARi
    

    Tags

    Cerulean and Venus

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    July 8, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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14. pSBbi-RP

    Plasmid

    #60513

    Purpose

    SB-transposon with constitutive bi-directional promoter, one side: SfiI cloning site for GOI (contains filler DNA), other side: RFP and puromycin resistance gene

    Depositor

    Eric Kowarz

    Article

    Kowarz et al Biotechnol J. 2015 Feb 4. doi: 10.1002/b

    Type

    Empty backbone

    Use

    Transposon

    Expression

    Mammalian

    Promoter

    EF1a/RPBSA

    Available Since

    Feb. 11, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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15. CP2-TCC

    Plasmid

    #179679

    Purpose

    Complementary plasmid

    Depositor

    David Liu

    Article

    Mok et al Nat Biotechnol. 2022 Sep;40(9):1378-1387

    Insert

    [sd2] T7 RNAP-TCC linker-degron
    

    Expression

    Bacterial

    Available Since

    April 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. pRDA_550

    Plasmid

    #203398

    Purpose

    All-in-one lentiviral expression of gRNA and EnAsCas12a

    Depositor

    John Doench

    Article

    Esmaeili Anvar et al Nat Commun. 2024 Apr 27;15(1):3577. doi:

    Insert

    Enhanced Acidaminococcus Cas12a
    

    Use

    CRISPR and Lentiviral

    Promoter

    EF1a

    Available Since

    Aug. 31, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. pGT-Ah10

    Plasmid

    #122577

    Purpose

    MAGIC donor plasmid (Himar transposon with tetQ/sfGFP payload)

    Depositor

    Harris Wang

    Article

    Ronda et al Nat Methods. 2019 Feb;16(2):167-170. doi

    Insert

    tetQ/sfGFP
    

    Use

    Synthetic Biology

    Expression

    Bacterial

    Available Since

    May 6, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. pTYB3-T4gp43(WT)

    Plasmid

    #228203

    Purpose

    Expression in BL21(DE3) of WT T4 DNA polymerase

    Depositor

    Stephen Benkovic

    Insert

    43
    

    Tags

    Sce VMA intein-chitin binding domain

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    May 22, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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19. CMV-Hylight

    Plasmid

    #193447

    Purpose

    A fluorescent biosensor for fructose 1,6-bisphosphate (FBP). Changes in FBP concentration strongly correlate with changes in glycolytic flux. (mammalian expression vector)

    Depositor

    Richard Goodman

    Article

    Koberstein et al Proc Natl Acad Sci U S A. 2022 Aug 2;119

    Insert

    Hylight Biosensor
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Nov. 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. pcDNA3.1his p66shc (corrected)

    Plasmid

    #32574

    Depositor

    Toren Finkel

    Article

    Nemoto et al Science. 2002 Mar 29. 295(5564):2450-2.

    Insert

    p66shc (SHC1 Human)
    

    Tags

    His

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Oct. 5, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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