We narrowed to 30,716 results for: URE

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pcDNA4-FLAG-Jhdm2a

Plasmid

#38136

Depositor

Toshinobu Nakamura, Toru Nakano

Article

Nakamura et al Nature. 2012 Jun 3;486(7403):415-9. doi:

Insert

Jhdm2a (Kdm3a Mouse)

Tags

FLAG

Expression

Mammalian

Mutation

protein starts at aa115

Promoter

CMV

Available Since

Sept. 28, 2012

Availability

Academic Institutions and Nonprofits only

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HR180-LGR5-iCT

Plasmid

#129094

Purpose

KI iCaspase9-tdTomato with a puromycin/mRuby selection cassette into human LGR5 locus

Depositor

Toshiro Sato

Article

Shimokawa et al Nature. 2017 May 11;545(7653):187-192. d

Insert

iCaspase9-T2A-tdTomato and LGR5 homology arms (LGR5 Human)

Use

Homologous recombination donor plasmid

Available Since

Sept. 30, 2019

Availability

Academic Institutions and Nonprofits only

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AAV phSyn1(S)-FLEX-tdTomato-WPRE

Plasmid

#51505

Purpose

Can be used to generate AAV virus that will express tdTomato in the presence of Cre in neurons from the synapsin promoter

Depositor

Hongkui Zeng

Article

Oh et al Nature. 2014 Apr 10;508(7495):207-14. do

Insert

tdTomato

Use

AAV

Promoter

phSyn1

Available Since

May 1, 2014

Availability

Academic Institutions and Nonprofits only

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pCDH-cGAS-E225A D227A-mCherry2-EF1-Puro

Plasmid

#132771

Purpose

Interacts with cytoplasmic DNA. Useful for identifying sites of nuclear envelope rupture.

Depositor

Jan Lammerding

Article

Denais et al Science. 2016 Apr 15;352(6283):353-8. do

Insert

Cyclic GMP-AMP synthase (CGAS Human)

Use

Lentiviral

Tags

mCherry2

Expression

Mammalian

Mutation

E225A D227A

Available Since

Jan. 3, 2020

Availability

Academic Institutions and Nonprofits only

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pAAV-CaMKIIa-hChR2(C128S/D156A)-mCherry

Plasmid

#35502

Purpose

AAV expression of CaMKII-driven stabilized step function opsin (SSFO) for optogenetic activation.

Depositor

Karl Deisseroth

Article

Yizhar et al Nature. 2011 Jul 27;477(7363):171-8. doi

Insert

hChR2(C128S/D156A)-mCherry

Use

AAV

Tags

mCherry

Expression

Mammalian

Mutation

C128S and D156A

Promoter

CaMKIIa

Available Since

April 18, 2012

Availability

Academic Institutions and Nonprofits only

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Human b4 nicotinic receptor subunit EM construct

Plasmid

#163961

Purpose

pEZT-BM vector with beta4 gene after CMV promoter, published EM construct

Depositor

Ryan Hibbs

Article

Gharpure et al Neuron. 2019 Nov 6;104(3):501-511.e6. do

Insert

Human beta4 nicotinic acetylcholine receptor subunit EM construct

Use

Baculovirus (bacmam) production and mammalian exp…

Tags

StrepII tag and apocytochrome b(562)RIL (BRIL)

Expression

Mammalian

Mutation

Pro341-Ser395 replaced with apocytochrome b(562)R…

Promoter

CMV

Available Since

Feb. 5, 2021

Availability

Academic Institutions and Nonprofits only

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Basigin-isoform2-CD4d3+4-bio

Plasmid

#73129

Purpose

EXPRESs plasmid for human erythrocyte surface proteins encoding Basigin-isoform2 with rat CD4d3+4-bio

Depositor

Gavin Wright

Article

Crosnier et al Nature. 2011 Nov 9;480(7378):534-7. doi:

Insert

Basigin isoform 2 (BSG Human)

Tags

biotinylation peptide and ratCD4d3+4

Expression

Mammalian

Promoter

CMV

Available Since

March 29, 2016

Availability

Academic Institutions and Nonprofits only

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bio-CD4d3+4-Kell blood group

Plasmid

#73089

Purpose

EXPRESs plasmid for human erythrocyte surface proteins encoding Kell blood group with bio-rat CD4d3+4

Depositor

Gavin Wright

Article

Crosnier et al Nature. 2011 Nov 9;480(7378):534-7. doi:

Insert

Kell blood group (KEL Human)

Tags

biotinylation peptide and ratCD4d3+4

Expression

Mammalian

Promoter

CMV

Available Since

March 28, 2016

Availability

Academic Institutions and Nonprofits only

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NAR4-CD4d3+4-bio

Plasmid

#73118

Purpose

EXPRESs plasmid for human erythrocyte surface proteins encoding NAR4 with rat CD4d3+4-bio

Depositor

Gavin Wright

Article

Crosnier et al Nature. 2011 Nov 9;480(7378):534-7. doi:

Insert

NAR4 (ART4 Human)

Tags

biotinylation peptide and ratCD4d3+4

Expression

Mammalian

Promoter

CMV

Available Since

March 29, 2016

Availability

Academic Institutions and Nonprofits only

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pAAV hSyn FLEx-loxp Kir2.1-2A-GFP

Plasmid

#161574

Purpose

Expression of Kir2.1-2A-GFP in a Cre-dependent fashion

Depositor

Kevin Beier, Robert Malenka

Article

Beier et al Nature. 2017 Sep 21;549(7672):345-350. d

Insert

Kir2.1-2A-GFP (Kcnj2 Mouse)

Use

AAV

Mutation

NA

Available Since

Nov. 20, 2020

Availability

Academic Institutions and Nonprofits only

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pAAV hSyn FLEx-OFF Kir2.1-2A-GFP

Plasmid

#161580

Purpose

Express Kir2.1 in cells not expressing Cre.

Depositor

Kevin Beier, Robert Malenka

Article

Beier et al Nature. 2017 Sep 21;549(7672):345-350. d

Insert

Kir2.1-2A-GFP (Kcnj2 Mouse)

Use

AAV

Mutation

NA

Available Since

Nov. 20, 2020

Availability

Academic Institutions and Nonprofits only

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AAV pCAG-FLEX-tdTomato-WPRE

Plasmid

#51503

Purpose

Can be used to generate AAV virus that will express tdTomato in the presence of Cre

Depositor

Hongkui Zeng

Article

Oh et al Nature. 2014 Apr 10;508(7495):207-14. do

Insert

tdTomato

Use

AAV

Promoter

pCAG

Available Since

Aug. 29, 2014

Availability

Academic Institutions and Nonprofits only

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MSCV-Puro-FH-AGO2-WT

Plasmid

#91986

Purpose

Expresses FLAG-HA-AGO2 (WT)

Depositor

Joshua Mendell

Article

Golden et al Nature. 2017 Feb 9;542(7640):197-202. do

Insert

AGO2 (AGO2 Human)

Use

Retroviral

Tags

FLAG-HA

Expression

Mammalian

Available Since

Aug. 25, 2017

Availability

Academic Institutions and Nonprofits only

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AAV phSyn1(S)-tdTomato-WPRE

Plasmid

#51506

Purpose

Can be used to generate AAV virus that will express tdTomato in neurons from the synapsin promoter

Depositor

Hongkui Zeng

Article

Oh et al Nature. 2014 Apr 10;508(7495):207-14. do

Has Service

AAV5

Insert

tdTomato

Use

AAV

Promoter

phSyn1

Available Since

Sept. 3, 2014

Availability

Academic Institutions and Nonprofits only

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FH-Tet2-pEF

Plasmid

#41710

Purpose

This plasmid expresses mouse Tet2 with N-terminal FLAG and HA tags in a mammalian system

Depositor

Anjana Rao

Article

Ko et al Nature. 2010 Dec 9;468(7325):839-43. doi

Insert

tet methylcytosine dioxygenase 2 (Tet2 Mouse)

Tags

FLAG and HA

Expression

Mammalian

Promoter

EF1a

Available Since

Feb. 18, 2014

Availability

Academic Institutions and Nonprofits only

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c-Myc-5-HT2A

Plasmid

#67944

Purpose

N-terminally c-myc tagged human 5-hydroxytryptamine receptor 2A

Depositor

Javier Gonzalez-Maeso

Article

Gonzalez-Maeso et al Nature. 2008 Mar 6;452(7183):93-7. doi:

Insert

HTR2A (HTR2A Human)

Tags

c-Myc

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 24, 2015

Availability

Academic Institutions and Nonprofits only

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GFP-PH-TAPP1

Plasmid

#161985

Purpose

Expresses GFP-tagged PH-Tapp1 in mammalian cells (PI(3,4)P2 biosensor)

Depositor

Dario Alessi, David Bryant

Article

Posor et al Nature. 2013 Jul 11;499(7457):233-7. doi

Insert

PH-TAPP1 (PLEKHA1 Human)

Tags

GFP

Expression

Mammalian

Available Since

Dec. 2, 2020

Availability

Academic Institutions and Nonprofits only

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pAAV-TREtight-mTagBFP2-B19G

Plasmid

#100799

Purpose

helper virus for monosynaptic tracing; to be coinjected with pAAV-syn-FLEX-splitTVA-EGFP-tTA

Depositor

Ian Wickersham

Article

Liu et al Nature. 2017 Aug 31;548(7669):582-587. d

Has Service

AAV1

Insert

mTagBFP2 and rabies virus SAD B19 strain glycoprotein genes (B19G)

Use

AAV

Expression

Mammalian

Promoter

TREtight

Available Since

Oct. 5, 2017

Availability

Academic Institutions and Nonprofits only

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pBRY-nuclear mCherry-IRES-PURO

Plasmid

#52409

Purpose

Constitutively expressed mammalian expression vector encoding nuclear mCherry fluorescent reporter. Puromycin resistance.

Depositor

Jacob Hanna

Article

Rais et al Nature. 2013 Oct 3;502(7469):65-70. doi:

Insert

nuclear mCherry

Expression

Mammalian

Available Since

May 29, 2014

Availability

Academic Institutions and Nonprofits only

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pLJM1-FH-AGO2-WT

Plasmid

#91978

Purpose

Expresses FLAG-HA-AGO2 (WT)

Depositor

Joshua Mendell

Article

Golden et al Nature. 2017 Feb 9;542(7640):197-202. do

Insert

AGO2 (AGO2 Human)

Use

Lentiviral

Tags

FLAG-HA

Expression

Mammalian

Available Since

Aug. 25, 2017

Availability

Academic Institutions and Nonprofits only

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