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Showing: 6221 - 6240 of 54780 results

  1. Epac-S-H96

    Plasmid
    #170342
    Purpose
    CFP(UST)Del_EPACdDEPCD_cp173Ven(ST)_Ven(ST) biosensor to measure realtime changes in FRET reflecting fluctuations in cAMP levels in living cells.
    Depositor
    Kees Jalink
    Article
    van der Krogt et al PLoS ONE. 2008 . 3(4):e1916.
    Insert
    CFP(UST)Del_EPACdDEPCD_cp173Ven(ST)_Ven(ST) (RAPGEF3 Human)
    Expression
    Mammalian
    Mutation
    Deletion of DEP domain, Catalytically dead T781A …
    Promoter
    CMV
    Available Since
    Oct. 5, 2021
    Availability
    Academic Institutions and Nonprofits only
    170342-plasmid-map-sequence-id-344641

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  5. delNC C-His Ana GvpC in pET28a

    Plasmid
    #85736
    Purpose
    Expresses a truncated His-tagged GvpC from Anabaena flos-aquae without the N-and C-terminal regions in E.coli
    Depositor
    Mikhail Shapiro
    Article
    Lakshmanan et al ACS Nano. 2016 Aug 23;10(8):7314-22. doi
    Insert
    Gas vesicle protein C (truncated and his-tagged)
    Tags
    His-tag
    Expression
    Bacterial
    Mutation
    Deletion of N and C terminal regions, addition of…
    Promoter
    T7
    Available Since
    March 29, 2017
    Availability
    Academic Institutions and Nonprofits only
    85736-plasmid-map-sequence-id-174840

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  8. pD10AH840AhCas9 (GB1041)

    Plasmid
    #68207
    Purpose
    Provides the human codon optimized CDS of Cas9 protein with mutated (D10A, H840A) and inactivated catalytic domains as a level 0 GoldenBraid part
    Depositor
    Diego Orzaez
    Article
    Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.
    Insert
    Cas9 coding region with mutated (D10A, H840A) and inactivated catalytic domains (human codon optimised)
    Use
    CRISPR and Synthetic Biology
    Mutation
    BsaI and BsmBI sites removed; human codon optimis…
    Available Since
    March 16, 2016
    Availability
    Academic Institutions and Nonprofits only
    68207-plasmid-map-sequence-id-135575

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  9. pBI121::chitGFP5:AQ

    Plasmid
    #240450
    Purpose
    Expression of indicator protein fusion (soluble modified GFP5 & Aequorin) for monitoring calcium concentrations in cell walls of higher plants. See Resource Information.
    Depositor
    Christoph Plieth
    Article
    Gao et al Plant Physiol. 2004 Mar;134(3):898-908.
    Insert
    Fusion of Chitinase signal, smGFP5, and Aequorin
    Expression
    Bacterial and Plant
    Mutation
    GFP5 for expression plants (PMID 9122158); Solubl…
    Promoter
    Cauliflower mosaic virus 35S promoter (GenBank AJ…
    Available Since
    Sept. 5, 2025
    Availability
    Academic Institutions and Nonprofits only
    240450-plasmid-map-sequence-id-485322

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  18. pET15b MOSPD2 RD/LD [282-490]

    Plasmid
    #186470
    Purpose
    Expression of human MOSPD2 (MSP domain from residue 282 to 490; mutant R404D/L406D, unable to bind FFAT motifs), fused to a 6HIS tag, in bacteria
    Depositor
    Catherine Tomasetto
    Article
    Di Mattia et al EMBO Rep. 2018 Jul;19(7). pii: embr.2017
    Insert
    MOSPD2 motile sperm domain containing 2 (MOSPD2 Human)
    Tags
    6HIS
    Expression
    Bacterial
    Mutation
    MSP domain [282-490]
    Available Since
    July 20, 2022
    Availability
    Academic Institutions and Nonprofits only
    186470-plasmid-map-sequence-id-367335

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Showing: 6221 - 6240 of 54780 results