We narrowed to 19,254 results for: T7

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pET30ax

Plasmid

#85761

Purpose

A bacterial expression vector with a His-tag at N and C-terminus.

Depositor

Lei Lu

Article

Madugula et al J Cell Sci. 2016 Oct 15;129(20):3922-393

Type

Empty backbone

Tags

His tag (N-term and C-term)

Expression

Bacterial

Promoter

T7 promoter

Available Since

May 8, 2017

Availability

Academic Institutions and Nonprofits only

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spNW100

Plasmid

#173488

Purpose

Expresses spNW100 in Bl21

Depositor

Huan Bao

Article

Zhang et al Nat Commun. 2021 Sep 14;12(1):5451. doi:

Insert

spNW100

Tags

Histag

Expression

Bacterial

Mutation

P1049L- please see depositor comments

Promoter

T7

Available Since

Sept. 15, 2021

Availability

Academic Institutions and Nonprofits only

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pBHRSF184

Plasmid

#89482

Purpose

Express asparaginyl endopeptidase 1 from O. affinis (OaAEP1)

Depositor

Hideo Iwai

Article

Mikula et al FEBS Lett. 2017 Mar 30. doi: 10.1002/187

Insert

Asparaginyl endopeptidase from Oldenlandia affinis

Tags

Smt3

Expression

Bacterial

Promoter

T7

Available Since

June 30, 2017

Availability

Academic Institutions and Nonprofits only

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pUC57-Simple-gRNA backbone

Plasmid

#51306

Purpose

gRNA expression plasmid

Depositor

Yonglong Chen

Article

Guo et al Development. 2014 Jan 8.

Type

Empty backbone

Use

CRISPR; Xenopus expression

Promoter

T7

Available Since

Feb. 11, 2014

Availability

Academic Institutions and Nonprofits only

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pET-53-RtcB

Plasmid

#51282

Purpose

E. coli expression vector for E. coli RtcB RNA ligase

Depositor

Jay Hesselberth

Article

Peach et al Nucleic Acids Res. 2015 May 22. pii: gkv

Insert

E. coli RtcB

Tags

6xHis

Expression

Bacterial

Promoter

T7 RNA Polymerase

Available Since

March 7, 2014

Availability

Academic Institutions and Nonprofits only

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pET N6his GFP1-9

Plasmid

#182240

Purpose

Tripartite split-GFP. E. coli vector. Expression of GFP1-9 detector fragment.

Depositor

Stéphanie Cabantous

Article

Pedelacq et al Methods Mol Biol. 2019;2025:423-437. doi

Insert

GFP1-9

Tags

6HIS

Expression

Bacterial

Promoter

T7

Available Since

April 26, 2022

Availability

Academic Institutions and Nonprofits only

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pET28a-Ec.coaA

Plasmid

#50386

Purpose

Heterologous expression of E. coli CoA biosynthetic enzyme, PanK (pantothenate kinase), induced with IPTG. Contain 6xHis-tag for nickel affinity purification.

Depositor

Erick Strauss

Article

Brand et al J Biol Chem. 2005 May 27;280(21):20185-8

Insert

PanK

Tags

6XHis-tag

Expression

Bacterial

Promoter

T7

Available Since

March 13, 2014

Availability

Academic Institutions and Nonprofits only

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pET11a-Z-NspGFP

Plasmid

#40729

Depositor

Brian McNaughton

Article

Blakeley et al Mol Biosyst. 2012 Aug;8(8):2036-40. Epub

Insert

N super positive GFP - Leucine Zipper

Tags

His

Expression

Bacterial

Promoter

T7

Available Since

Nov. 26, 2012

Availability

Academic Institutions and Nonprofits only

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pET_RTX

Plasmid

#102787

Purpose

Expresses the Reverse Transcriptase gene

Depositor

Andrew Ellington

Article

Ellefson et al Science. 2016 Jun 24;352(6293):1590-3. d

Insert

reverse transcription xenopolymerase

Expression

Bacterial

Promoter

T7 promoter + lac operator

Available Since

July 3, 2018

Availability

Academic Institutions and Nonprofits only

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pT7-gRNA

Plasmid

#46759

Purpose

gRNA cloning vector for in vitro transcription of target gRNA

Depositor

Wenbiao Chen

Article

Jao et al Proc Natl Acad Sci U S A. 2013 Aug 5.

Insert

gRNA core

Use

CRISPR; Zebrafish expression

Promoter

T7

Available Since

Aug. 2, 2013

Availability

Academic Institutions and Nonprofits only

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pACYC-GFPM2

Plasmid

#102791

Purpose

Expresses GFP under WT T7 promoter

Depositor

Andrew Ellington

Article

Abil et al Nat Protoc. 2017 Dec;12(12):2493-2512. d

Insert

Green Fluorescent Protein

Expression

Bacterial

Available Since

July 3, 2018

Availability

Academic Institutions and Nonprofits only

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pET23a-mUbc9

Plasmid

#53137

Purpose

E.coli expression of full length untagged mouse Ubc9 (protein sequence identical to human)

Depositor

Frauke Melchior

Article

Pichler et al Cell. 2002 Jan 11;108(1):109-20.

Insert

Ubc9 (Ube2i Mouse)

Tags

untagged

Expression

Bacterial

Mutation

protein sequence is identical to human

Promoter

T7

Available Since

Sept. 17, 2014

Availability

Academic Institutions and Nonprofits only

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pLysscys255

Plasmid

#36429

Depositor

Emine Gunhan, Pete Lollar

Article

Gunhan et al Protein Expr Purif. 2008 Apr;58(2):203-9

Insert

Saporin-3/cys255

Expression

Bacterial

Mutation

cys added at position 255

Promoter

T7

Available Since

May 10, 2012

Availability

Academic Institutions and Nonprofits only

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pET-6xHis-(pos15)GFP

Plasmid

#89248

Purpose

[Edit] Expresses GFP with +15 theoretical charge in E.Coli

Depositor

David Liu

Article

Thompson et al Chem Biol. 2012 Jul 27;19(7):831-43. doi

Insert

His6-(pos15)-GFP

Tags

His6

Expression

Bacterial

Promoter

T7

Available Since

April 21, 2017

Availability

Academic Institutions and Nonprofits only

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6xHis-MBP-huMb2Cas12a

Plasmid

#174676

Purpose

Expresses 6xHis-MBP tagged Mb2Cas12a

Depositor

Piyush Jain

Article

Nguyen et al medRxiv 2021.07.21

Insert

huMb2Cas12a

Use

CRISPR and Synthetic Biology

Tags

6xHis, MBP, TEV and NLS, 6xHis, 3xHA

Expression

Bacterial

Promoter

T7

Available Since

Oct. 22, 2021

Availability

Academic Institutions and Nonprofits only

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Hox A11a

Plasmid

#36462

Depositor

Robert Ho

Article

Ahn et al Dev Biol. 2008 Oct 1;322(1):220-33. Epub

Insert

hoxa11a (hoxa11a Zebrafish)

Use

Riboprobe synthesis

Promoter

T7

Available Since

Sept. 28, 2012

Availability

Academic Institutions and Nonprofits only
pET22b-CPDBamHI-Leu

Plasmid

#38256

Depositor

Matthew Bogyo, Aimee Shen

Article

Shen et al PLoS One. 2009 Dec 2;4(12):e8119.

Type

Empty backbone

Tags

6xHis and Vibrio cholerae MARTX toxin cysteine pr…

Expression

Bacterial

Promoter

T7

Available Since

Aug. 1, 2012

Availability

Academic Institutions and Nonprofits only

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pET21a.MJ0135

Plasmid

#11412

Depositor

Sung-Hou Kim

Article

Lai et al Structure. 2000 Aug 15. 8(8):897-904.

Insert

RNase HII (MJ_RS00700 M. jannaschii)

Expression

Bacterial

Promoter

T7

Available Since

April 10, 2006

Availability

Academic Institutions and Nonprofits only

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V72 ELP

Plasmid

#68938

Purpose

Elastin-like Polypeptide (VPGxG) with 72 repeats containing Valine guest residue

Depositor

Andrew MacKay

Article

Dhandhukia et al Biomacromolecules. 2013 Apr 8;14(4):976-

Insert

V72 ELP

Expression

Bacterial

Promoter

T7

Available Since

Oct. 2, 2015

Availability

Academic Institutions and Nonprofits only

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pRSET-mSA-EGFP

Plasmid

#39862

Depositor

Sheldon Park

Article

Lim et al Biotechnol Bioeng. 2013 Jan;110(1):57-67

Insert

monomeric streptavidin fused to EGFP

Tags

6xHis, EGFP, and FLAG

Expression

Bacterial

Promoter

T7

Available Since

March 15, 2013

Availability

Academic Institutions and Nonprofits only

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