We narrowed to 9,378 results for: mel

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EGFP-Hu_Plk5(W207F)

Plasmid

#136320

Purpose

Mammalian expression of human Plk5 W207F with N-terminal EGFP

Depositor

Guillermo de Cárcer, Marcos Malumbres

Article

de Carcer et al Mol Cell Biol. 2011 Mar;31(6):1225-39. d

Insert

PLK5 (PLK5 Human)

Expression

Mammalian

Mutation

W207F

Available Since

Aug. 10, 2020

Availability

Academic Institutions and Nonprofits only

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INCTbiosyn-pUB-HspINT4

Plasmid

#127513

Purpose

Plasmid encodes H. sapiens codon optimized Integrase 4.

Depositor

Martin Bonamino

Article

Gomide et al Commun Biol. 2020 May 22;3(1):255. doi:

Insert

Integrase 4 coding sequence codon optimized for H. sapiens expression.

Expression

Mammalian

Available Since

May 6, 2020

Availability

Academic Institutions and Nonprofits only

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INCTbiosyn-pUB-HspINT5

Plasmid

#127514

Purpose

Plasmid encodes H. sapiens codon optimized Integrase 5.

Depositor

Martin Bonamino

Article

Gomide et al Commun Biol. 2020 May 22;3(1):255. doi:

Insert

Integrase 5 coding sequence codon optimized for H. sapiens expression.

Expression

Mammalian

Available Since

May 6, 2020

Availability

Academic Institutions and Nonprofits only

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pBADHISTEV_PfeEH258A

Plasmid

#129122

Purpose

expresses H258A mutant of PfeE in E.coli

Depositor

James Naismith

Article

Perraud et al ACS Chem Biol. 2018 Sep 21;13(9):2603-26

Insert

Periplasmic Esterase PfeE (PA2689 Pseudomonas aeruginosa PAO1)

Tags

TEV protease cleavable 6xHis

Expression

Bacterial

Mutation

Signal peptide sequence has been removed, mutat…

Promoter

PBAD

Available Since

Aug. 26, 2019

Availability

Academic Institutions and Nonprofits only

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pBADHISTEV_PfeES157A

Plasmid

#129121

Purpose

expresses S157A mutant of PfeE in E.coli

Depositor

James Naismith

Article

Perraud et al ACS Chem Biol. 2018 Sep 21;13(9):2603-26

Insert

Periplasmic Esterase PfeE (PA2689 Pseudomonas aeruginosa PAO1)

Tags

TEV protease cleavable 6xHis

Expression

Bacterial

Mutation

Signal peptide sequence has been removed, mutat…

Promoter

PBAD

Available Since

Aug. 21, 2019

Availability

Academic Institutions and Nonprofits only

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Dscam1_7.27.26 EC10-Fc-His

Plasmid

#72189

Purpose

Expresses the 10 N-terminal extracellular domains of the Dscam, isoform 7.27.26 protein, C-terminally fused to the Fc region of human IgG1 + 6X histidine tag.

Depositor

Woj Wojtowicz

Article

Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

Insert

Dscam1_7.27.26

Tags

Fc-His

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 15, 2019

Availability

Academic Institutions and Nonprofits only

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Dscam1_7.27.23 EC10-Fc-His

Plasmid

#72187

Purpose

Expresses the 10 N-terminal extracellular domains of the Dscam, isoform 7.27.23 protein, C-terminally fused to the Fc region of human IgG1 + 6X histidine tag.

Depositor

Woj Wojtowicz

Article

Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

Insert

Dscam1_7.27.23

Tags

Fc-His

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 15, 2019

Availability

Academic Institutions and Nonprofits only

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Dscam1_7.27.26 EC10-AP-His

Plasmid

#72063

Purpose

Expresses the 10 N-terminal extracellular domains of the Dscam, isoform 7.27.26 protein, C-terminally fused to alkaline phosphatase + 6X histidine tag.

Depositor

Woj Wojtowicz

Article

Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

Insert

Dscam1_7.27.26

Tags

AP-His

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 15, 2019

Availability

Academic Institutions and Nonprofits only

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GAL-ARF

Plasmid

#89121

Purpose

mammalian expression of p14-hARF linked to GAL4 DNA binding domain

Depositor

Elizabeth Wilson

Article

Minges et al J Biol Chem. 2015 Oct 9;290(41):25174-87

Insert

p14-hARF (CDKN2A Human)

Tags

GAL4 DBD

Expression

Mammalian

Available Since

April 10, 2017

Availability

Academic Institutions and Nonprofits only

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Dscam1_7.27.30 EC10-AP-His

Plasmid

#72064

Purpose

Expresses the 10 N-terminal extracellular domains of the Dscam, isoform 7.27.30 protein, C-terminally fused to alkaline phosphatase + 6X histidine tag.

Depositor

Woj Wojtowicz

Article

Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

Insert

Dscam1_7.27.30

Tags

AP-His

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 23, 2016

Availability

Academic Institutions and Nonprofits only

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Dscam1_7.27.22 EC10-AP-His

Plasmid

#72060

Purpose

Expresses the 10 N-terminal extracellular domains of the Dscam, isoform 7.27.22 protein, C-terminally fused to alkaline phosphatase + 6X histidine tag.

Depositor

Woj Wojtowicz

Article

Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

Insert

Dscam1_7.27.22

Tags

AP-His

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 23, 2016

Availability

Academic Institutions and Nonprofits only

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Dscam1_7.27.23 EC10-AP-His

Plasmid

#72061

Purpose

Expresses the 10 N-terminal extracellular domains of the Dscam, isoform 7.27.23 protein, C-terminally fused to alkaline phosphatase + 6X histidine tag.

Depositor

Woj Wojtowicz

Article

Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

Insert

Dscam1_7.27.23

Tags

AP-His

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 18, 2016

Availability

Academic Institutions and Nonprofits only

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Dscam1_7.27.22 EC10-Fc-His

Plasmid

#72186

Purpose

Expresses the 10 N-terminal extracellular domains of the Dscam, isoform 7.27.22 protein, C-terminally fused to the Fc region of human IgG1 + 6X histidine tag.

Depositor

Woj Wojtowicz

Article

Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

Insert

Dscam1_7.27.22

Tags

Fc-His

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 25, 2016

Availability

Academic Institutions and Nonprofits only

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pUAST-HA, Pbl-A DH domain

Plasmid

#69794

Purpose

Pebble isoform A, DH domain, in P element-based pUAST vector for Gal4-regulated expression in Drosophila.

Depositor

Arno Muller

Article

van Impel et al Development. 2009 Mar;136(5):813-22. doi

Insert

Pebble DH domain

Use

P element-based puast vector for gal4-regulated e…

Tags

HA-Tag

Expression

Insect

Mutation

Contains only the Pebble DH domain

Promoter

hsp70

Available Since

Oct. 7, 2015

Availability

Academic Institutions and Nonprofits only
shCTL-mlpx-neo

Plasmid

#65233

Purpose

encodes a non-targeting control shRNA

Depositor

Gerardo Ferbeyre

Article

Deschenes-Simard et al Genes Dev. 2013 Apr 15;27(8):900-15. doi

Insert

non-coding shRNA

Expression

Mammalian

Promoter

PGK

Available Since

Aug. 12, 2015

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-ADAM7 (S703N)

Plasmid

#31141

Depositor

Yardena Samuels

Article

Wei et al Hum Mutat. 2011 Jun;32(6):E2148-75. doi:

Insert

ADAM7 (ADAM7 Human)

Tags

FLAG

Expression

Mammalian

Mutation

S703N

Available Since

July 22, 2011

Availability

Academic Institutions and Nonprofits only
pET28a-MED20

Plasmid

#15435

Depositor

Joan Conaway, Ronald Conaway

Article

Sato et al J Biol Chem. 2003 Apr 25. 278(17):15123-

Insert

MED20 (Med20 Mouse)

Tags

His

Expression

Bacterial

Mutation

N/A

Available Since

Aug. 10, 2007

Availability

Academic Institutions and Nonprofits only
pRK5_mEGFP-SS18-SSX2

Plasmid

#237637

Purpose

For overexpression of mEGFP-SS18-SSX2

Depositor

Denes Hnisz

Article

Zhang et al Nature. 2025 Jun 4. doi: 10.1038/s41586-

Insert

SS18-SSX2 (SS18, SSX2 Human)

Tags

mEGFP

Expression

Mammalian

Available Since

May 6, 2025

Availability

Academic Institutions and Nonprofits only

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pLX311-GFP-MEKDD

Plasmid

#194882

Purpose

Expression of dominant negative MEK1

Depositor

Sefi Rosenbluh

Article

Tuano et al Genome Biol. 2023 Mar 29;24(1):59. doi:

Insert

MEKDD (MAP2K1 Human)

Use

Lentiviral

Expression

Mammalian

Mutation

S218D, S222D

Promoter

E1Fa

Available Since

March 31, 2023

Availability

Academic Institutions and Nonprofits only

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pDONR223-MEKDD

Plasmid

#31202

Purpose

Gateway entry vector containing MEKDD.

Depositor

David Root

Article

Yang et al Nat Methods. 2011 Jun 26;8(8):659-61. do

Insert

MAPKK1 (MAP2K1 Human)

Use

Gateway Cloning

Mutation

S218D S222D

Available Since

June 24, 2011

Availability

Academic Institutions and Nonprofits only

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