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AiP14530: pAAV-AiE0743m_3xC2-minBG-mTFP1-WPRE3-BGHpA (Alias: CN4530)

Plasmid

#230869

Purpose

AiE0743m_3xC2 is an enhancer sequence, designed to drive AAV-mediated transgene expression in striatal cholinergic interneurons

Depositor

Allen Institute for Brain Science, Jonathan Ting

Article

Hunker et al Neuron. 2025 May 21;113(10):1507-1524.e1

Insert

mTFP1

Use

AAV

Expression

Mammalian

Available Since

Jan. 29, 2025

Availability

Academic Institutions and Nonprofits only

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AiP12909: pAAV-AiE0445h_3xC2-minBG-SYFP2-WPRE3-BGHpA (Alias: CN2909)

Plasmid

#230862

Purpose

AiE0445h_3xC2 is an enhancer sequence, designed to drive AAV-mediated transgene expression in striatal indirect pathway MSNs (D2-MSNs)

Depositor

Allen Institute for Brain Science, Jonathan Ting

Article

Hunker et al Neuron. 2025 May 21;113(10):1507-1524.e1

Insert

SYFP2

Use

AAV

Expression

Mammalian

Available Since

Jan. 29, 2025

Availability

Academic Institutions and Nonprofits only

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AiP14646: pAAV-AiE0779m_6xC2-minBG-jGCaMP8s-WPRE3-BGHpA (Alias: CN4646)

Plasmid

#230872

Purpose

AiE0779m_6xC2 is an enhancer sequence, designed to drive AAV-mediated transgene expression in striatal direct pathway MSNs (D1-MSNs)

Depositor

Allen Institute for Brain Science, Jonathan Ting

Article

Hunker et al Neuron. 2025 May 21;113(10):1507-1524.e1

Insert

jGCaMP8s

Use

AAV

Expression

Mammalian

Available Since

Jan. 29, 2025

Availability

Academic Institutions and Nonprofits only

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AiP14531: pAAV-hDLX_C2.1-minBG-mTFP1-WPRE3-BGHpA (Alias: CN4531)

Plasmid

#230870

Purpose

hDLX_C2.1 is an enhancer sequence, designed to drive AAV-mediated transgene expression in striatal cholinergic interneurons

Depositor

Allen Institute for Brain Science, Jonathan Ting

Article

Hunker et al Neuron. 2025 May 21;113(10):1507-1524.e1

Insert

mTFP1

Use

AAV

Expression

Mammalian

Available Since

Jan. 29, 2025

Availability

Academic Institutions and Nonprofits only

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AiP14133: pAAV-AiE0445h_3xC2-minBG-jGCaMP8m-WPRE3-BGHpA (Alias: CN4133)

Plasmid

#230867

Purpose

AiE0445h_3xC2 is an enhancer sequence, designed to drive AAV-mediated transgene expression in striatal indirect pathway MSNs (D2-MSNs)

Depositor

Allen Institute for Brain Science, Jonathan Ting

Article

Hunker et al Neuron. 2025 May 21;113(10):1507-1524.e1

Insert

jGCaMP8m

Use

AAV

Expression

Mammalian

Available Since

Jan. 29, 2025

Availability

Academic Institutions and Nonprofits only

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AiP15582: pAAV-AiE0780m-minBG-jRGECO1a-WPRE3-BGHpA (Alias: CN5582)

Plasmid

#230878

Purpose

AiE0780m is an enhancer sequence, designed to drive AAV-mediated transgene expression in striatal direct pathway MSNs (D1-MSNs)

Depositor

Allen Institute for Brain Science, Jonathan Ting

Article

Hunker et al Neuron. 2025 May 21;113(10):1507-1524.e1

Insert

jRGECO1a

Use

AAV

Expression

Mammalian

Available Since

Jan. 27, 2025

Availability

Academic Institutions and Nonprofits only

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AiP15645: pAAV-AiE1426m_3xC1-minBG-SYFP2-WPRE3-BGHpA (Alias: CN5645)

Plasmid

#230883

Purpose

AiE1426m_3xC1 is an enhancer sequence, designed to drive AAV-mediated transgene expression in striatal Sst-Chodl neurons

Depositor

Allen Institute for Brain Science, Jonathan Ting

Article

Hunker et al Neuron. 2025 May 21;113(10):1507-1524.e1

Insert

SYFP2

Use

AAV

Expression

Mammalian

Available Since

Jan. 27, 2025

Availability

Academic Institutions and Nonprofits only

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AiP15746: pAAV-AiE0780m-minBG-mScarlet-WPRE3-BGHpA (Alias: CN5746)

Plasmid

#230881

Purpose

AiE0780m is an enhancer sequence, designed to drive AAV-mediated transgene expression in striatal direct pathway MSNs (D1-MSNs)

Depositor

Allen Institute for Brain Science, Jonathan Ting

Article

Hunker et al Neuron. 2025 May 21;113(10):1507-1524.e1

Insert

mScarlet

Use

AAV

Expression

Mammalian

Available Since

Jan. 27, 2025

Availability

Academic Institutions and Nonprofits only

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AiP12614 - pAAV-AiE0785m-minBG-SYFP2-WPRE3-BGHpA (Alias: CN2614)

Plasmid

#224168

Purpose

AiE0785m is an enhancer sequence, designed to drive AAV-mediated transgene expression in striatal MSNs

Depositor

Allen Institute for Brain Science, Jonathan Ting

Article

Hunker et al Neuron. 2025 May 21;113(10):1507-1524.e1

Insert

SYFP2

Use

AAV

Available Since

Sept. 26, 2024

Availability

Academic Institutions and Nonprofits only

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AiP14826: pAAV-AiE0441h_3xC2-minBG-FlpO-WPRE3-BGHpA (Alias: CN4826)

Plasmid

#214863

Purpose

Expression of FlpO in striatal medium spiny neurons

Depositor

Allen Institute for Brain Science, Jonathan Ting

Article

Hunker et al Neuron. 2025 May 21;113(10):1507-1524.e1

Insert

FlpO

Use

AAV

Available Since

July 15, 2024

Availability

Academic Institutions and Nonprofits only

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AiP14036: pAAV-AiE0743m_3xC2-minBG-CoChR-EGFP-WPRE3-BGHpA (Alias: CN4036)

Plasmid

#214854

Purpose

Expression of CoChR-EGFP in striatal cholinergic neurons

Depositor

Allen Institute for Brain Science, Jonathan Ting

Article

Hunker et al Neuron. 2025 May 21;113(10):1507-1524.e1

Insert

CoChR-EGFP

Use

AAV

Available Since

July 15, 2024

Availability

Academic Institutions and Nonprofits only

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AiP13118 - pAAV-AiE0367h_C2-minBG-tdTomato-WPRE3-BGHpA (Alias: CN3118)

Plasmid

#191730

Purpose

AI plasmid ALIAS: AiP13118. Expression of tdTomato in striatal medium spiny neurons

Depositor

Allen Institute for Brain Science, Jonathan Ting

Article

Hunker et al Neuron. 2025 May 21;113(10):1507-1524.e1

Insert

tdTomato

Use

AAV

Mutation

none

Available Since

Feb. 1, 2024

Availability

Academic Institutions and Nonprofits only

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AiP13185 - pAAV-AiE0447h-minBG-dTomato-WPRE3-BGHpA (Alias: CN3185)

Plasmid

#191731

Purpose

AI plasmid ALIAS: AiP13185. Expression of dTomato in striatal medium spiny neurons

Depositor

Allen Institute for Brain Science, Jonathan Ting

Article

Hunker et al Neuron. 2025 May 21;113(10):1507-1524.e1

Insert

tdTomato

Use

AAV

Mutation

none

Available Since

Feb. 1, 2024

Availability

Academic Institutions and Nonprofits only

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AAVS1-pur-CAG-Bi-DREADD

Plasmid

#159457

Purpose

AAVS1 targeting donor plasmid with Bi-DREADD expression cassette (hM3Dq-mCherry-P2A-HA-KORD) and puromycin selection gene

Depositor

Yuejun Chen

Article

Xiong et al Cell Stem Cell. 2020 Sep 16. pii: S1934-

Insert

Bi-DREADD

Expression

Mammalian

Mutation

fusion protein of hM3Dq-mCherry and HA-KORD seper…

Promoter

CAG

Available Since

Dec. 8, 2020

Availability

Academic Institutions and Nonprofits only

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pLCKO2

Plasmid

#125518

Purpose

Lentiviral backbone for cloning and expressing U6 driven sgRNAs with BsmBI cloning sites and puromycin selection.

Depositor

Jason Moffat

Article

Mair et al Cell Rep. 2019 Apr 9;27(2):599-615.e12.

Type

Empty backbone

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6

Available Since

June 28, 2019

Availability

Academic Institutions and Nonprofits only

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Nsp3 -EGFP

Plasmid

#165108

Purpose

mammalian expression and localization

Depositor

Bruno Antonny

Article

Miserey-Lenkei et al Biol Cell. 2021 Jul;113(7):311-328. doi:

Insert

Nsp3 (ORF1ab SARS-COV-2)

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 11, 2021

Availability

Academic Institutions and Nonprofits only

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pGL4.0-TERT G228A

Plasmid

#84926

Purpose

luciferase reporter for mutant TERT promoter

Depositor

Joseph Costello

Article

Bell et al Science. 2015 May 29;348(6238):1036-9. d

Insert

TERT promoter (TERT Human)

Use

Luciferase

Tags

luciferase

Expression

Mammalian

Mutation

G228A

Promoter

TERT

Available Since

March 8, 2017

Availability

Academic Institutions and Nonprofits only

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Nsp4-mCherry

Plasmid

#165132

Purpose

mammalian expression and localization

Depositor

Bruno Antonny

Article

Miserey-Lenkei et al Biol Cell. 2021 Jul;113(7):311-328. doi:

Insert

Nsp4 (ORF1ab codon optimized SARS-COV-2)

Tags

mCherry

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 18, 2021

Availability

Academic Institutions and Nonprofits only

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ATG-3925 (CBG2)

Plasmid

#167839

Purpose

Expresses CBG2 (Click Beetle Green 2) in E. coli and mammalian cells

Depositor

Lance Encell

Article

Zambito et al iScience. 2020 Dec 26;24(1):101986. doi:

Insert

Click Beetle Green 2

Tags

6-His

Expression

Bacterial and Mammalian

Mutation

Q348H. Click Beetle Green 2 (CBR2) has 3 amino a…

Promoter

CMV, T7

Available Since

June 16, 2021

Availability

Academic Institutions and Nonprofits only

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FLAG-Rheb (Q64L)-pcw107

Plasmid

#64607

Purpose

when used to produce lentivirus, express physiological levels of insert

Depositor

David Sabatini, Kris Wood

Article

Martz et al Sci Signal. 2014 Dec 23;7(357):ra121. do

Insert

RHEB (RHEB Human)

Use

Lentiviral

Tags

FLAG

Mutation

Q64L

Promoter

PGK

Available Since

May 12, 2015

Availability

Academic Institutions and Nonprofits only