We narrowed to 19,051 results for: Tec;

1. pCellFree_G03 HDAC1

   Plasmid

   #67101

   Purpose

   Standard for cell-free expression benchmarking.

   Depositor

   Kirill Alexandrov

   Article

   Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

   Insert

   HDAC1 (HDAC1 Human)
   

   Use

   Cell-free expression

   Tags

   EGFP and His

   Promoter

   T7

   Available Since

   Nov. 6, 2015

   Availability

   Academic Institutions and Nonprofits only

2. pCfB3050(gRNA XII-5)

   Plasmid

   #73292

   Purpose

   EasyClone-MarkerFree guiding RNA vector to direct Cas9 to cut at site XII-5

   Depositor

   Irina Borodina

   Article

   Jessop-Fabre et al Biotechnol J. 2016 Aug;11(8):1110-7. doi

   Insert

   guiding RNA
   

   Use

   CRISPR; Grna

   Expression

   Yeast

   Available Since

   Nov. 23, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pcDNA5/FRT/TO-globin_del5UTR_PTC39-xrRNA-4H

   Plasmid

   #108376

   Purpose

   FRT integration of beta-globin reporter with premature termination codon (PTC39); enables detection of 5'-3' decay intermediates (xrFrag) and allows detection via northern blot (4H binding sites)

   Depositor

   Niels Gehring

   Article

   Boehm et al Nat Commun. 2016 Dec 5;7:13691. doi: 10.

   Insert

   PTC39 beta-globin reporter with MVE xrRNA and 4H probe binding sites
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   May 1, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pOCC121

   Plasmid

   #118893

   Purpose

   shuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with N-terminal MBP tag, cleavable with 3C, and monomeric GFP and C-terminal HIS6, cleavable with 3C

   Depositor

   Marc Bickle, PEPC MPI CBG

   Article

   Lemaitre et al BMC Biotechnol. 2019 Mar 29;19(1):20. do

   Insert

   NcoI-MBP-3C-mGFP-NotI-ccdB-AscI-3C-HIS6-stop-HindIII cassette
   

   Tags

   HIS6, cleavable with 3C protease and MBP, cleavab…

   Expression

   Insect

   Promoter

   polH

   Available Since

   Jan. 2, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. CP1041-ABE8e-dimer

   Plasmid

   #138499

   Purpose

   A-to-G base editor

   Depositor

   David Liu

   Article

   Richter et al Nat Biotechnol. 2020 Jul;38(7):883-891.

   Insert

   ecTadA(WT)-ecTadA(8e)-nSpCas9 (CP1041)
   

   Expression

   Mammalian

   Available Since

   March 16, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. TAL2272

   Plasmid

   #36709

   Depositor

   Keith Joung

   Article

   Reyon et al Nat Biotechnol. 2012 Apr 8. doi: 10.1038

   Insert

   KRAS-TALEN_Left (Kras Human)
   

   Use

   Mammalian, t7 for in vitro mrna synthesis

   Tags

   3X Flag and WT FOKI

   Promoter

   CMV

   Available Since

   June 26, 2012

   Availability

   Academic Institutions and Nonprofits only

7. Zebrafish-gRNA-0008

   Plasmid

   #42248

   Purpose

   gRNA targeted to zebrafish gene tia1l

   Depositor

   Keith Joung

   Article

   Hwang et al Nat Biotechnol. 2013 Jan 29. doi: 10.103

   Insert

   gRNA-tia1l (tia1l Zebrafish)
   

   Use

   CRISPR; Zebrafish expression

   Promoter

   T7

   Available Since

   Jan. 31, 2013

   Availability

   Academic Institutions and Nonprofits only

8. TAL2273

   Plasmid

   #36710

   Depositor

   Keith Joung

   Article

   Reyon et al Nat Biotechnol. 2012 Apr 8. doi: 10.1038

   Insert

   KRAS-TALEN_Right (Kras Human)
   

   Use

   Mammalian, t7 for in vitro mrna synthesis

   Tags

   3X Flag and WT FOKI

   Promoter

   CMV

   Available Since

   June 26, 2012

   Availability

   Academic Institutions and Nonprofits only

9. pOCC70

   Plasmid

   #118878

   Purpose

   shuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with C-terminal monomeric GFP, cleavable with TEV, and C-terminal HIS6 tag, cleavable with 3C

   Depositor

   Marc Bickle, PEPC MPI CBG

   Article

   Lemaitre et al BMC Biotechnol. 2019 Mar 29;19(1):20. do

   Insert

   NcoI-NotI-ccdB-AscI-TEV-mGFP-3C-HIS6-stop-HindIII cassette
   

   Tags

   mGFP, cleavable with TEV protease; HIS6, cleavabl…

   Expression

   Insect

   Promoter

   polH

   Available Since

   Jan. 3, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pGWB526

    Plasmid

    #74868

    Purpose

    Gateway cloning compatible binary vector for C-terminal fusion with T7 (CaMV35S promoter).

    Depositor

    Tsuyoshi Nakagawa

    Article

    Nakagawa et al Biosci Biotechnol Biochem. 2007 Aug;71(8

    Type

    Empty backbone

    Expression

    Plant

    Promoter

    CaMV35S

    Available Since

    July 14, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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11. SaKKH-ABE8e-dimer

    Plasmid

    #138503

    Purpose

    A-to-G base editor

    Depositor

    David Liu

    Article

    Richter et al Nat Biotechnol. 2020 Jul;38(7):883-891.

    Insert

    ecTadA(WT)-ecTadA(8e)-nSaCas9 (KKH)
    

    Expression

    Mammalian

    Available Since

    March 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. L2-AA012

    Plasmid

    #185751

    Purpose

    Vector to introduce eGFP under control of pAaEF1a into the genome of the hornwort Anthoceros agrestis via Agrobacterium mediated transformation. eGFP contains a cell membrane localization tag.

    Depositor

    Peter Szoevenyi

    Article

    Frangedakis et al New Phytol. 2021 Jun 2. doi: 10.1111/nph

    Inserts

    HygR2
    eGFP
    

    Tags

    membrane localization tag Lti6b

    Expression

    Plant

    Available Since

    July 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. tdEos-Vimentin-N-18

    Plasmid

    #57690

    Purpose

    Localization: Intermediate Filaments, Excitation: 505 / 569, Emission: 516 / 581

    Depositor

    Michael Davidson

    Article

    Rizzo et al Cold Spring Harb Protoc. 2009 Dec;2009(1

    Insert

    NM_003380.3 (VIM Human)
    

    Tags

    tdEos

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Jan. 13, 2015

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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14. pUPD2 AtUBQ10 (GB2478)

    Plasmid

    #160594

    Purpose

    UBQ10 promoter from A. thaliana for constitutive expression in plant systems.

    Depositor

    Diego Orzaez

    Article

    Bernabe-Orts et al Plant Biotechnol J. 2019 Apr 5. doi: 10.

    Insert

    AtUBQ10
    

    Use

    CRISPR and Synthetic Biology

    Mutation

    BsaI and BsmBI sites removed

    Available Since

    March 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pCellFree_G03 NFKB1

    Plasmid

    #67126

    Purpose

    Standard for cell-free expression benchmarking.

    Depositor

    Kirill Alexandrov

    Article

    Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

    Insert

    NFKB1 (NFKB1 Human)
    

    Use

    Cell-free expression

    Tags

    EGFP and His

    Promoter

    T7

    Available Since

    Nov. 6, 2015

    Availability

    Academic Institutions and Nonprofits only

16. CTA2-mNG211

    Plasmid

    #141167

    Purpose

    Expresses GST-SUMO tagged CTA2 Subunit with 11th Strand

    Depositor

    Wayne Lencer

    Article

    Luong et al Mol Biol Cell. 2019 Nov 27:mbcE19070375.

    Insert

    SUMO-CTA2-mNG211
    

    Tags

    SUMO

    Expression

    Bacterial

    Available Since

    July 31, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCfB3041(gRNA X-3)

    Plasmid

    #73283

    Purpose

    EasyClone-MarkerFree guiding RNA vector to direct Cas9 to cut at site X-3

    Depositor

    Irina Borodina

    Article

    Jessop-Fabre et al Biotechnol J. 2016 Aug;11(8):1110-7. doi

    Insert

    guiding RNA
    

    Use

    CRISPR; Grna

    Expression

    Yeast

    Available Since

    Nov. 23, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. LbABE8e-dimer

    Plasmid

    #138505

    Purpose

    A-to-G base editor

    Depositor

    David Liu

    Article

    Richter et al Nat Biotechnol. 2020 Jul;38(7):883-891.

    Insert

    ecTadA(WT)-ecTadA(8e)-dLbCas12a
    

    Expression

    Mammalian

    Available Since

    March 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pRSET FLIPglu-600uDelta11 Aphrodite

    Plasmid

    #13569

    Depositor

    Wolf Frommer

    Article

    Deuschle et al Plant Cell. 2006 Sep . 18(9):2314-25.

    Insert

    FLIPglu F16A Delta11 Aphrodite
    

    Tags

    Aphrodite and ECFP

    Expression

    Bacterial

    Mutation

    Fluorophore-derived linker shortened. F16A mutati…

    Available Since

    March 2, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pCellFree_G03 DDX5

    Plasmid

    #67111

    Purpose

    Standard for cell-free expression benchmarking.

    Depositor

    Kirill Alexandrov

    Article

    Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

    Insert

    DDX5 (DDX5 Human)
    

    Use

    Cell-free expression

    Tags

    EGFP and His

    Promoter

    T7

    Available Since

    Nov. 6, 2015

    Availability

    Academic Institutions and Nonprofits only