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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 10,083 results for: pET

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Showing: 6281 - 6300 of 10083 results

  1. pHIT521

    Plasmid
    #204520
    Purpose
    Bacterial expression of CEC-5 N-terminal fragment with 6xHis and maltose binding protein tags for in vitro assays
    Depositor
    Yuji Kohara
    Publication
    Tabara et al EMBO J. 2023 Jun 1;42(11):e105002. doi:
    Insert
    N-terminal fragment of cec-5 (C. elegans)
    Tags
    6xHis, MBP
    Expression
    Bacterial
    Promoter
    T7lac
    Available since
    Aug. 10, 2023
    Availability
    Academic Institutions and Nonprofits only
    204520-plasmid-map-sequence-id-403749

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  2. pHIT182

    Plasmid
    #204511
    Purpose
    Bacterial expression of catalytically defective CSR-1a with 6xHis and maltose binding protein tags for in vitro assays
    Depositor
    Yuji Kohara
    Publication
    Aoki et al EMBO J. 2007 Dec 12. 26(24):5007-19.
    Insert
    csr-1a with D769A mutation (C. elegans) (csr-1 Nematode)
    Tags
    6xHis, MBP
    Expression
    Bacterial
    Mutation
    D769A
    Promoter
    T7lac
    Available since
    Aug. 10, 2023
    Availability
    Academic Institutions and Nonprofits only
    204511-plasmid-map-sequence-id-403747

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  3. pHIT520

    Plasmid
    #204519
    Purpose
    Bacterial expression of CEC-8 N-terminal fragment with 6xHis and maltose binding protein tags for in vitro assays
    Depositor
    Yuji Kohara
    Publication
    Tabara et al EMBO J. 2023 Jun 1;42(11):e105002. doi:
    Insert
    N-terminal fragment of cec-8 (C. elegans) (Y55B1BR.3 Nematode)
    Tags
    6xHis, MBP
    Expression
    Bacterial
    Promoter
    T7lac
    Available since
    Aug. 10, 2023
    Availability
    Academic Institutions and Nonprofits only
    204519-plasmid-map-sequence-id-403744

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  4. pHIT342

    Plasmid
    #204514
    Purpose
    Bacterial expression of SYP-1 N-terminal fragment with 6xHis and maltose binding protein tags for use as an antigen
    Depositor
    Yuji Kohara
    Publication
    Tabara et al EMBO J. 2023 Jun 1;42(11):e105002. doi:
    Insert
    N-terminal fragment of syp-1 (C. elegans)
    Tags
    6xHis, MBP
    Expression
    Bacterial
    Promoter
    T7lac
    Available since
    Aug. 10, 2023
    Availability
    Academic Institutions and Nonprofits only
    204514-plasmid-map-sequence-id-403743

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  5. pHIT320

    Plasmid
    #204517
    Purpose
    Bacterial expression of HIM-1 internal fragment with 6xHis and maltose binding protein tags for use as an antigen
    Depositor
    Yuji Kohara
    Publication
    Tabara et al EMBO J. 2023 Jun 1;42(11):e105002. doi:
    Insert
    Internal fragment of him-1 (C. elegans) (him-1 Nematode)
    Tags
    6xHis, MBP
    Expression
    Bacterial
    Promoter
    T7lac
    Available since
    Aug. 10, 2023
    Availability
    Academic Institutions and Nonprofits only
    204517-plasmid-map-sequence-id-403742

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  9. GB1-ΔN-CHMP4C(S191C,I225A,L228A,W231A)

    Plasmid
    #199242
    Purpose
    Codon-optimized bacterial expression plasmid. Expresses GB1-His6-TEV-CHMP4C(121-233, S191C,I225A,L228A,W231A)
    Depositor
    Lalit Deshmukh
    Publication
    Elias et al Sci Adv. 2023 Jul 14;9(28):eadg3913. doi
    Insert
    GB1-His6-TEV-CHMP4C(121-233, S191C, I225A, L228A, W231A) (CHMP4C Human)
    Tags
    GB1-His6-TEV
    Expression
    Bacterial
    Mutation
    S191C, I225A, L228A, W231A
    Available since
    June 5, 2023
    Availability
    Academic Institutions and Nonprofits only
    199242-plasmid-map-sequence-id-393310

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  20. pSN-A112C-1A32

    Plasmid
    #191241
    Purpose
    Expresses nuclease A-H124L from Staphylococcus aureus, with an A112C mutation, fused through a flexible linker to the transmembrane domain of poly-Leu-1A32 and a His-tag, for fluorescent studies.
    Depositor
    Alessandro Senes
    Publication
    Diaz Vazquez et al Biophys J. 2023 Jan 3;122(1):143-155. do
    Insert
    nuc (nuclease A), HLA-A (HLAA) (predicted interfacial residues of the transmembrane domain in a poly-leu background) (HLA-A Human, Staphylococcus aureus)
    Tags
    nuclease A from S. aureus fused to the poly-Leu T…
    Expression
    Bacterial
    Mutation
    Changed Alanine 112 to Cysteine in SN, and predic…
    Promoter
    T7 promoter
    Available since
    Feb. 15, 2023
    Availability
    Academic Institutions and Nonprofits only
    191241-plasmid-map-sequence-id-384667

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Showing: 6281 - 6300 of 10083 results