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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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Showing: 6301 - 6320 of 19677 results

  4. pColE1_sgRNA_M4NS_AmpR

    Plasmid
    #119156
    Purpose
    Encodes sgRNA targeting position 6 in pColE1_70a_deGFP_KanR with four mismatches in the non-seed region
    Depositor
    Vincent Noireaux
    Publication
    Collias et al Synth Biol (Oxf). 2019 Jan 21;4(1):ysz00
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    sgRNA targeting position 6 in pColE1_70a_deGFP_KanR, with four mismatches in the non-seed region
    Use
    Crispr
    Promoter
    J23119
    Available since
    Nov. 28, 2018
    Availability
    Academic Institutions and Nonprofits only
    119156-plasmid-map-sequence-id-229445

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  8. NSP6-Flag

    Plasmid
    #210345
    Purpose
    Expresses SARS-CoV-2 NSP6 fused with Flag in mammalian cells
    Depositor
    Michele Pagano
    Publication
    Li et al J Virol. 2023 Sep 28:e0050723. doi: 10.1
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    SARS-CoV-2 NSP6 (ORF1ab Human, SARS-CoV-2 isolate Wuhan-Hu-1)
    Tags
    Flag
    Expression
    Mammalian
    Mutation
    Many synonymous changes due to codon optimization
    Available since
    Jan. 3, 2024
    Availability
    Academic Institutions and Nonprofits only
    210345-plasmid-map-sequence-id-418203

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  9. pAAV.CAG.FLEX.iGluSnFR3.v857.PDGFR

    Plasmid
    #196219
    Purpose
    Fluorescent reporter for glutamate, third generation, variant 857 in PDGFR backbone. iGluSnFR3.v857.PDGFR
    Depositor
    Kaspar Podgorski
    Publication
    Aggarwal et al Nat Methods. 2023 May 4. doi: 10.1038/s4
    Has service
    AAV2
    Insert
    pAAV CAG FLEX iGluSnFR3 v857.PDGFR
    Use
    AAV, Cre/Lox, Mouse Targeting, and Synthetic Biol…
    Tags
    IgK-chain, Myc epi tag, and PDGFR TM Domain
    Expression
    Mammalian
    Available since
    Aug. 24, 2023
    Availability
    Academic Institutions and Nonprofits only
    196219-plasmid-map-sequence-id-405480

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  10. pcDNA5-FRT-TO-Nsp8-Nsp7-HF_GC3opt

    Plasmid
    #157702
    Purpose
    mammalian expression of C-terminally His8-Flag tagged SARS-CoV-2 Nsp8-Nsp7 fusion protein under control of a tetracycline-inducible promoter
    Depositor
    David Tollervey
    Publication
    Bresson et al Wellcome Open Res. 2020 Nov 3;5:261. doi
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Nsp8-Nsp7-HF_GC3opt (ORF1ab SARS-CoV-2)
    Tags
    His8-Flag
    Expression
    Mammalian
    Mutation
    codon optimized; gene expression was optimized by…
    Available since
    Aug. 5, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
    157702-plasmid-map-sequence-id-307866

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  11. pCRISPR-Cas9/CD4-TK2

    Plasmid
    #104397
    Purpose
    The designed sgRNA cloned into this plasmid directs the specific DNA cleavage exerted by Cas9 nuclease in a region of exon 5 of the human TK2 gene. The plasmid also includes the Cas9 nuclease and CD4.
    Depositor
    Jeronimo Blanco
    Publication
    Meca-Cortes et al Mol Ther Nucleic Acids. 2017 Sep 15;8:39
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    TK2 (TK2 Human)
    Expression
    Mammalian
    Promoter
    U6
    Available since
    April 13, 2018
    Availability
    Academic Institutions and Nonprofits only
    104397-plasmid-map-sequence-id-208338

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  12. pSPneogRNA241510+MT

    Plasmid
    #84292
    Purpose
    Express LdPBK_241510.1 and LdMT targeting gRNAs simutaneously
    Depositor
    Greg Matlashewski
    Publication
    Zhang et al mSphere. 2017 Jan 18;2(1). pii: e00340-1
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    LdBPK_241510.1 targeting gRNA and LdMT targeting gRNA
    Use
    CRISPR; Leishmania donovani
    Promoter
    L. donovani ribosome RNA promoter
    Available since
    Jan. 30, 2017
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    84292-plasmid-map-sequence-id-160871

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  18. hCD12

    Plasmid
    #225541
    Purpose
    Expresses human histone deacetylase 6 (HDAC6) catalytic domain 1 and 2 (CD12), also known as hCD12, in Escherichia coli cells.
    Depositor
    David Christianson
    Publication
    Hai et al Nat Chem Biol. 2016 Sep;12(9):741-7. doi
    Insert
    Human Histone Deacetylase 6 Catalytic Domain 1 and 2 (HDAC6 Human)
    Tags
    His6-MBP tag with cleavable TEV site
    Expression
    Bacterial
    Promoter
    T7
    Available since
    Sept. 24, 2024
    Availability
    Academic Institutions and Nonprofits only
    225541-plasmid-map-sequence-id-446604

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  19. pSilencer2.1-U6-3JB8F+12

    Plasmid
    #190854
    Purpose
    For mammalian expression of 3JB8F+12, a dual-specificity aptamer that binds OGT and β-catenin. Expression is driven by a U6 promoter. This dual-specificity aptamer adopts a folded linker.
    Depositor
    Gerald Hart
    Publication
    Zhu et al Cell. 2022 Dec 30:S0092-8674(22)01529-X.
    Insert
    3JB8F_T1+12bp (A Dual-Specificity aptamer targeting OGT and β-catenin)
    Use
    Affinity Reagent/ Antibody and Synthetic Biology
    Expression
    Mammalian
    Promoter
    U6
    Available since
    Jan. 9, 2023
    Availability
    Industry, Academic Institutions, and Nonprofits
    190854-plasmid-map-sequence-id-379261

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Showing: 6301 - 6320 of 19677 results