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psiCHECK-2-Fhod1-3'UTR

Plasmid

#61773

Purpose

Luciferase reporter containing the 3'UTR of mouse Fhod1

Depositor

Judy Lieberman

Publication

Perdigao-Henriques et al Oncogene. 2015 Mar 23. doi: 10.1038/onc.

Insert

mouse Fhod1 3'UTR

Use

Luciferase

Available since

May 14, 2015

Availability

Academic Institutions and Nonprofits only
psiCHECK-2-Mapk12-3'UTR

Plasmid

#61783

Purpose

Luciferase reporter containing the 3'UTR of mouse Mapk12

Depositor

Judy Lieberman

Publication

Perdigao-Henriques et al Oncogene. 2015 Mar 23. doi: 10.1038/onc.

Insert

mouse Mapk12 3'UTR

Use

Luciferase

Available since

May 14, 2015

Availability

Academic Institutions and Nonprofits only
LI B-C N-NLS KOZ c2

Plasmid

#54443

Depositor

Martin Parniske

Publication

Binder et al PLoS One. 2014 Feb 13;9(2):e88218. doi:

Insert

Nuclear localization signal (NLS) of SV40 large T antigen

Use

Cloning vector

Available since

Nov. 20, 2014

Availability

Academic Institutions and Nonprofits only

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pCS2 xenopus DAN

Plasmid

#24693

Depositor

Edward De Robertis

Publication

Pera et al Int J Dev Biol. 2005 . 49(7):781-96.

Insert

DAN (nbl1 Frog)

Expression

Mammalian

Available since

June 7, 2010

Availability

Academic Institutions and Nonprofits only
pPS1117

Plasmid

#1356

Depositor

Pamela Silver

Publication

Seedorf et al Proc Natl Acad Sci U S A 1997 Aug 5;94(1

Insert

SXM1 (SXM1 Budding Yeast)

Tags

sGFP

Expression

Yeast

Mutation

*

Available since

Aug. 3, 2005

Availability

Academic Institutions and Nonprofits only
YY229: pAAV_TRE::GFP- GEMINI(A)-P1N4_hSyn::rtTA3G

Plasmid

#228889

Purpose

AAV plasmid expressing the destabilized GFP-GEMINI(A) under the control of a doxycycline-inducible promotor, and rtTA3G under the control of a hSyn promotor

Depositor

Dingchang Lin

Publication

Yan et al Nature. 2026 Mar 3. doi: 10.1038/s41586-

Insert

GFP-GEMINI(A); rtTA3G

Use

AAV

Tags

gfp

Promoter

TRE; hsyn

Available since

Feb. 3, 2026

Availability

Academic Institutions and Nonprofits only

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pYO1031

Plasmid

#235706

Purpose

Expression of FL human Beclin 1 and FL human UVRAG in mammalian cells

Depositor

Roger Williams

Publication

Ohashi et al Elife. 2020 Jun 30;9:e58281. doi: 10.755

Inserts

Beclin 1 (BECN1 Human)
UVRAG (UVRAG Human)

Expression

Mammalian

Promoter

CAG and CMV

Available since

May 13, 2025

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pYO1125

Plasmid

#235742

Purpose

Protein expression of GST-tagged PX domain (Cys at 2) in bacteria

Depositor

Roger Williams

Publication

Rostislavleva et al Science. 2015 Oct 9;350(6257):aac7365. d

Insert

p40 PX domain (NCF4 Human)

Tags

GST-TEV

Expression

Bacterial

Mutation

aa 2-149 with Cys at 2

Available since

April 9, 2025

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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LVDP 2P.2

Plasmid

#231904

Purpose

For the production of SARS-CoV-2 virus-like particles (VLPs) in the '2-plasmid' system and packaging firefly luciferase mRNA into the VLPs

Depositor

Sriram Neelamegham

Publication

Yang et al Npj Viruses. 2025 Jan 25;3(1):4. doi: 10

Insert

nucleocapsid protein-T2A-membrane protein-IRES-envelope protein (N, E, M SARS-CoV-2)

Use

Lentiviral

Mutation

R203M in N protein

Promoter

CMV, EF-1a

Available since

Jan. 31, 2025

Availability

Academic Institutions and Nonprofits only

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R2pH-LAMPx3ALFA

Plasmid

#225136

Purpose

R2pH-LAMP1-labelled lysosomes with 3xLFA

Depositor

Massimiliano Stagi

Publication

Cheetham-Wilkinson et al Sci Adv. 2025 Jan 3;11(1):eadr7325. doi:

Insert

Lamp1 (Lamp1 Mouse)

Expression

Mammalian

Available since

Jan. 6, 2025

Availability

Academic Institutions and Nonprofits only

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pAG27

Plasmid

#226742

Purpose

Expresses a reporter for a mitochondrial matrix protein

Depositor

Jonathan Weissman

Publication

Muthukumar et al Mol Cell. 2024 Mar 21;84(6):1101-1119.e9

Insert

COX4 (COX4I1 Human)

Use

Lentiviral

Tags

GFP1-10

Available since

Oct. 24, 2024

Availability

Academic Institutions and Nonprofits only

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pME-H2B-V5-n2StayGoldC4 (JDW 1354)

Plasmid

#224486

Purpose

Gateway compatible middle entry clone contianing Histone H2B fusion to V5-tagged (n2)StayGold(C4) (Nuclear StayGold fluorescent reporter)

Depositor

Joshua Wythe

Publication

Gillespie WB et al Development. 2025 Nov 15;152(22):dev2043

Insert

H2B-V5-n2StayGoldC4

Use

Gateway Cloning

Available since

Sept. 30, 2024

Availability

Academic Institutions and Nonprofits only

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pME-mScarlet3-Giantin (JDW 1356)

Plasmid

#224502

Purpose

Gateway compatible middle entry clone containing a V5-tagged mScarlet fused to Giantin (For visualizing the Golgi)

Depositor

Joshua Wythe

Publication

Gillespie WB et al Development. 2025 Nov 15;152(22):dev2043

Insert

mScarlet3-Giantin

Use

Gateway Cloning

Available since

Sept. 27, 2024

Availability

Academic Institutions and Nonprofits only

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pME-myc-BirA (JDW 1210)

Plasmid

#224509

Purpose

Gateway compatible middle entry clone containing a Myc tagged BirA

Depositor

Joshua Wythe

Publication

Gillespie WB et al Development. 2025 Nov 15;152(22):dev2043

Insert

Myc-BirA

Use

Gateway Cloning

Available since

Sept. 26, 2024

Availability

Academic Institutions and Nonprofits only

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p3E-H2A_mCherry_SV40pA (JDW 967)

Plasmid

#224528

Purpose

A Gateway compatible 3' entry clone containing an H2A mCherry fusion followed by SV40 late polyA

Depositor

Joshua Wythe

Publication

Gillespie WB et al Development. 2025 Nov 15;152(22):dev2043

Insert

H2A-mCherry-SV40pA

Use

Gateway Cloning

Available since

Sept. 26, 2024

Availability

Academic Institutions and Nonprofits only

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p3E-V5-mScarlet-I_SV40pA (JDW 968)

Plasmid

#224529

Purpose

A Gateway compatible 3' entry clone containing an V5 mScarlet fusion followed by SV40 late polyA

Depositor

Joshua Wythe

Publication

Gillespie WB et al Development. 2025 Nov 15;152(22):dev2043

Insert

V5-mScarlet-I

Use

Gateway Cloning

Available since

Sept. 26, 2024

Availability

Academic Institutions and Nonprofits only

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pDONR221-Lifeact- mScarlet-HA (JDW 1247)

Plasmid

#224538

Purpose

A Gateway compatible middle entry clone containing Lifeact-mScarlet-I (far red f-actin reporter)

Depositor

Joshua Wythe

Publication

Gillespie WB et al Development. 2025 Nov 15;152(22):dev2043

Insert

Lifeact-mScarlet-HA

Use

Gateway Cloning

Available since

Sept. 26, 2024

Availability

Academic Institutions and Nonprofits only

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pF(UG) hSyn SYT7alpha

Plasmid

#179713

Purpose

Lentiviral expression of mouse Syt7

Depositor

Edwin Chapman

Publication

Vevea et al Elife. 2021 Sep 20;10. pii: 67261. doi:

Insert

SYT7 (Syt7 Mouse)

Use

Lentiviral

Available since

Sept. 3, 2024

Availability

Academic Institutions and Nonprofits only

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H2B-Electra2

Plasmid

#218929

Purpose

Electra2 fused to human H2B protein for nuclear localization

Depositor

Amro Hamdoun

Insert

H2B-Electra2 (H2B Entacmaea quadricolor, Human, Synthetic)

Use

Synthetic Biology

Tags

Electra2 and H2B

Expression

Mammalian

Available since

Aug. 6, 2024

Availability

Academic Institutions and Nonprofits only

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FRB-mCherry-EEA1

Plasmid

#222419

Purpose

To express an early endosome "anchor" for rapamycin-induced heterodimerisation in mammalian cells

Depositor

Stephen Royle

Publication

Downie et al PLoS Biol. 2025 Jul 10;23(7):e3003249. d

Insert

EEA1 (EEA1 Human)

Tags

FRB-mCherry

Expression

Mammalian

Available since

July 16, 2024

Availability

Academic Institutions and Nonprofits only

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