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A1-LCD-6R

Plasmid

#178665

Purpose

Bacterial expression of N-terminally 6His tagged A1-LCD with six Arg residues removed (LCD: low-complexity domain, aa 186-320)

Depositor

Tanja Mittag

Article

Bremer et al Nat Chem. 2022 Feb;14(2):196-207. doi: 1

Insert

A1-LCD-6R (HNRNPA1 Human)

Tags

6xHis

Expression

Bacterial

Mutation

six Arg residues removed

Available Since

March 9, 2022

Availability

Academic Institutions and Nonprofits only

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A1-LCD+2R

Plasmid

#178666

Purpose

Bacterial expression of N-terminally 6His tagged A1-LCD with two Arg residues added (LCD: low-complexity domain, aa 186-320)

Depositor

Tanja Mittag

Article

Bremer et al Nat Chem. 2022 Feb;14(2):196-207. doi: 1

Insert

A1-LCD+2R (HNRNPA1 Human)

Tags

6xHis

Expression

Bacterial

Mutation

two Arg residues added

Available Since

March 9, 2022

Availability

Academic Institutions and Nonprofits only

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A1-LCD-6R+6K

Plasmid

#178670

Purpose

Bacterial expression of N-terminally 6His tagged A1-LCD with six Arg residues replaced with Lys (LCD: low-complexity domain, aa 186-320)

Depositor

Tanja Mittag

Article

Bremer et al Nat Chem. 2022 Feb;14(2):196-207. doi: 1

Insert

A1-LCD-6R+6K (HNRNPA1 Human)

Tags

6xHis

Expression

Bacterial

Mutation

six Arg residues replaced with Lys

Available Since

March 8, 2022

Availability

Academic Institutions and Nonprofits only

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A1-LCD+12D

Plasmid

#178675

Purpose

Bacterial expression of N-terminally 6His tagged A1-LCD with twelve Asp residues added (LCD: low-complexity domain, aa 186-320)

Depositor

Tanja Mittag

Article

Bremer et al Nat Chem. 2022 Feb;14(2):196-207. doi: 1

Insert

A1-LCD+12D (HNRNPA1 Human)

Tags

6xHis

Expression

Bacterial

Mutation

twelve Asp residues added

Available Since

March 8, 2022

Availability

Academic Institutions and Nonprofits only

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A1-LCD-3R+3K

Plasmid

#178669

Purpose

Bacterial expression of N-terminally 6His tagged A1-LCD with three Arg residues replaced with Lys (LCD: low-complexity domain, aa 186-320)

Depositor

Tanja Mittag

Article

Bremer et al Nat Chem. 2022 Feb;14(2):196-207. doi: 1

Insert

A1-LCD-3R+3K (HNRNPA1 Human)

Tags

6xHis

Expression

Bacterial

Mutation

three Arg residues replaced with Lys

Available Since

March 8, 2022

Availability

Academic Institutions and Nonprofits only

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A1-LCD+7F-7Y

Plasmid

#178660

Purpose

Bacterial expression of N-terminally 6His tagged A1-LCD with seven Tyr residues replaced with Phe (LCD: low-complexity domain, aa 186-320)

Depositor

Tanja Mittag

Article

Bremer et al Nat Chem. 2022 Feb;14(2):196-207. doi: 1

Insert

A1-LCD +7F-7Y (HNRNPA1 Human)

Tags

6xHis

Expression

Bacterial

Mutation

seven Tyr residues replaced with Phe

Available Since

March 8, 2022

Availability

Academic Institutions and Nonprofits only

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A1-LCD+12E

Plasmid

#178676

Purpose

Bacterial expression of N-terminally 6His tagged A1-LCD with twelve Glu residues added (LCD: low-complexity domain, aa 186-320)

Depositor

Tanja Mittag

Article

Bremer et al Nat Chem. 2022 Feb;14(2):196-207. doi: 1

Insert

A1-LCD+12E (HNRNPA1 Human)

Tags

6xHis

Expression

Bacterial

Mutation

twelve Glu residues added

Available Since

March 8, 2022

Availability

Academic Institutions and Nonprofits only

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pME-MmCav1

Plasmid

#170527

Purpose

Entry clone for tagging MmCav1 at the C terminus. Parton lab clone BCJ

Depositor

Rob Parton

Article

Rae et al Elife. 2021 Apr 27;10. pii: 64630. doi:

Insert

Caveolin1 (Cav1 Mouse)

Use

Multisite gateway entry clone

Available Since

Sept. 8, 2021

Availability

Academic Institutions and Nonprofits only

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Pet22b-YAPL318E

Plasmid

#166444

Purpose

Bacterial expression of 6x His tagged YAPL318E

Depositor

Shuguo Sun

Article

Yu et al Mol Cell. 2021 Feb 13. pii: S1097-2765(2

Insert

YAPL318E (YAP1 Human)

Expression

Bacterial

Available Since

April 7, 2021

Availability

Academic Institutions and Nonprofits only

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Pet22b-YAP4LE

Plasmid

#166445

Purpose

Bacterial expression of 6x His tagged YAP4LE

Depositor

Shuguo Sun

Article

Yu et al Mol Cell. 2021 Feb 13. pii: S1097-2765(2

Insert

YAP4LE (YAP1 Human)

Expression

Bacterial

Available Since

April 7, 2021

Availability

Academic Institutions and Nonprofits only

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KLC1N343S-Myc

Plasmid

#166963

Purpose

Expresses Myc-tagged KLC1 N343S protein in pcDNA3.1

Depositor

Rosalind Segal

Article

Fukuda et al J Cell Biol. 2021 Jan 4;220(1). pii: 211

Insert

KLC1-N343S (Klc1 Mouse)

Tags

Myc

Expression

Mammalian

Mutation

KLC1-N343S

Promoter

CMV

Available Since

March 18, 2021

Availability

Academic Institutions and Nonprofits only

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pLL3.7-EF-EYFP-YAP1_R89A-PolyA

Plasmid

#112291

Purpose

Fluorescently labels human YAP1-2gamma isoform (504 aa) R89A mutant

Depositor

Erik Sahai

Article

Ege et al Cell Syst. 2018 Jun 27;6(6):692-708.e13.

Insert

EYFP-YAP1 (YAP1 Human)

Use

Lentiviral

Mutation

Human YAP1-2gamma with Arginine 89 to Alanine

Promoter

EF-1 alpha

Available Since

May 13, 2019

Availability

Academic Institutions and Nonprofits only

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pLL3.7-EF-EYFP-YAP1_L91A-PolyA

Plasmid

#112292

Purpose

Fluorescently labels human YAP1-2gamma isoform (504 aa) L91A mutant

Depositor

Erik Sahai

Article

Ege et al Cell Syst. 2018 Jun 27;6(6):692-708.e13.

Insert

EYFP-YAP1 (YAP1 Human)

Use

Lentiviral

Mutation

Human YAP1-2gamma with Leucine 91 to Alanine

Promoter

EF-1 alpha

Available Since

May 13, 2019

Availability

Academic Institutions and Nonprofits only

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pLL3.7-EF-EYFP-YAP1_F95A-PolyA

Plasmid

#112293

Purpose

Fluorescently labels human YAP1-2gamma isoform (504 aa) F95A mutant

Depositor

Erik Sahai

Article

Ege et al Cell Syst. 2018 Jun 27;6(6):692-708.e13.

Insert

EYFP-YAP1 (YAP1 Human)

Use

Lentiviral

Mutation

Human YAP1-2gamma with Phenylalanine 95 to Alanine

Promoter

EF-1 alpha

Available Since

May 13, 2019

Availability

Academic Institutions and Nonprofits only

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pBABE (hygro) hYAP 1-2delta

Plasmid

#124154

Purpose

Protein expression for funtional studies of cell growth promotion

Depositor

Megan Finch-Edmondson, Marius Sudol

Article

Gaffney et al Gene. 2012 Nov 10;509(2):215-22. doi: 10

Insert

YAP1 (YAP1 Human)

Use

Unspecified

Mutation

None (Wild Type)

Available Since

April 29, 2019

Availability

Academic Institutions and Nonprofits only

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pBABE (hygro) hYAP 1-1alpha

Plasmid

#124147

Purpose

Protein expression for funtional studies of cell growth promotion

Depositor

Megan Finch-Edmondson, Marius Sudol

Article

Gaffney et al Gene. 2012 Nov 10;509(2):215-22. doi: 10

Insert

YAP1 (YAP1 Human)

Use

Unspecified

Mutation

None (Wild Type)

Available Since

April 29, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pBABE (hygro) hYAP 1-1beta

Plasmid

#124148

Purpose

Protein expression for funtional studies of cell growth promotion

Depositor

Megan Finch-Edmondson, Marius Sudol

Article

Gaffney et al Gene. 2012 Nov 10;509(2):215-22. doi: 10

Insert

YAP1 (YAP1 Human)

Use

Unspecified

Mutation

None (Wild Type)

Available Since

April 29, 2019

Availability

Academic Institutions and Nonprofits only

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pBABE (hygro) hYAP 1-1gamma

Plasmid

#124149

Purpose

Protein expression for funtional studies of cell growth promotion

Depositor

Megan Finch-Edmondson, Marius Sudol

Article

Gaffney et al Gene. 2012 Nov 10;509(2):215-22. doi: 10

Insert

YAP1 (YAP1 Human)

Use

Unspecified

Mutation

None (Wild Type)

Available Since

April 29, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pBABE (hygro) hYAP 1-2alpha

Plasmid

#124151

Purpose

Protein expression for funtional studies of cell growth promotion

Depositor

Megan Finch-Edmondson, Marius Sudol

Article

Gaffney et al Gene. 2012 Nov 10;509(2):215-22. doi: 10

Insert

YAP1 (YAP1 Human)

Use

Unspecified

Mutation

None (Wild Type)

Available Since

April 29, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pBABE (hygro) hYAP 1-2beta

Plasmid

#124152

Purpose

Protein expression for funtional studies of cell growth promotion

Depositor

Megan Finch-Edmondson, Marius Sudol

Article

Gaffney et al Gene. 2012 Nov 10;509(2):215-22. doi: 10

Insert

YAP1 (YAP1 Human)

Use

Unspecified

Mutation

None (Wild Type)

Available Since

April 29, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge