We narrowed to 58,607 results for: plasmid

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GfaABC1D BARK D110A p2A mCherry

Plasmid

#117692

Purpose

GfaABC1D-iBARK(D110A)-p2A-mCherry: Negative control viral expression vector for astrocyte Gaq silencing

Depositor

Baljit Khakh

Article

Nagai et al Neuron. 2021 Jul 21;109(14):2256-2274.e9

Insert

BARKrgs D110A peptide

Use

AAV

Tags

HA / FLAG

Mutation

D110A

Available Since

May 28, 2021

Availability

Academic Institutions and Nonprofits only

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pAAV_hsyn_NES-his-CaMPARI2-F391W-G395D-WPRE-SV40

Plasmid

#101063

Purpose

AAV vector expressing CaMPARI2_F391W-G395D (Kd = 530nM), a photoconvertible fluorescent protein-based calcium integrator, in neurons

Depositor

Eric Schreiter

Article

Moeyaert et al Nat Commun. 2018 Oct 25;9(1):4440. doi:

Insert

CaMPARI2_F391W-G395D

Use

AAV

Tags

FLAG-HA-myc and NES_his

Promoter

hsyn (synapsin-1)

Available Since

Oct. 2, 2017

Availability

Academic Institutions and Nonprofits only

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AAV2/9 REP-AAP-ΔCAP

Plasmid

#205991

Purpose

Rep and AAP protein expression for AAV CREATE libraries

Depositor

Viviana Gradinaru

Article

Chuapoco et al Nat Nanotechnol. 2023 Jul 10. doi: 10.10

Insert

AAV9 Rep and AAP proteins for CREATE libraries

Use

AAV

Available Since

Aug. 18, 2023

Availability

Academic Institutions and Nonprofits only

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Lentiviral mBmal1-Luc

Plasmid

#182762

Purpose

Lentiviral vector encoding the promoter region of mouse Bmal1 gene driving a luciferase reporter

Depositor

Qing-Jun Meng

Article

Gossan et al Arthritis Rheum. 2013 Sep;65(9):2334-45.

Insert

mouse arntl promoter (Bmal1 Mouse)

Use

Lentiviral

Promoter

649 bp

Available Since

Sept. 8, 2022

Availability

Academic Institutions and Nonprofits only

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pGL2-Mcl-1 mut

Plasmid

#19133

Depositor

Wafik El-Deiry

Article

Ricci et al Cancer Cell. 2007 Jul . 12(1):66-80.

Insert

Mcl-1 promoter (MCL1 Human)

Use

Luciferase

Expression

Mammalian

Mutation

NFkB binding-site mutant, the sequence GGGGTCTTCC…

Available Since

Oct. 2, 2008

Availability

Academic Institutions and Nonprofits only

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pMK435

Plasmid

#236573

Purpose

Cloning vector for PaqCI Golden Gate assembly of ISYmu1 gRNAs into TRV2 Architecture_B

Depositor

Steven Jacobsen

Article

Weiss et al Nat Plants. 2025 Apr 22. doi: 10.1038/s4

Type

Empty backbone

Expression

Plant

Available Since

April 30, 2025

Availability

Academic Institutions and Nonprofits only

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pLY100-RetroEFS-HER2-28BBz-WPRE

Plasmid

#192201

Purpose

Retro-HER2CAR

Depositor

Sidi Chen

Article

Ye et al Cell Metab. 2022 Apr 5;34(4):595-614.e14

Insert

Retro-HER2CAR (ERBB2 Synthetic)

Use

Retroviral; Mammalian expression

Mutation

NA

Available Since

Nov. 1, 2022

Availability

Academic Institutions and Nonprofits only

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AAV-EFS-SaABE8eV106W-bGH-U6-sgRNA-BsmBI

Plasmid

#189924

Purpose

AAV genome encoding SaABE8eV106W and Sa sgRNA cassette on a single AAV, with BsmBI sites for protospacer installation

Depositor

David Liu

Article

Davis et al Nat Biomed Eng. 2022 Jul 28. pii: 10.103

Insert

SaABE8e V106W

Use

AAV

Mutation

SaCas9 D10A, TadA ABE8e V106W

Promoter

EFS

Available Since

Sept. 7, 2022

Availability

Academic Institutions and Nonprofits only

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PB-CAG-NLS-dCas9-HA-2XNLS-10xGCN4-NLS-P2A-scFv-NLS-P65-HSF1-Flag-WPRE-PolyA-PGK-EM7-NeoR-polyA

Plasmid

#168290

Purpose

CRISPR-activator Suntag-P65-HSF1 (SPH)

Depositor

Hui Yang

Article

Gao et al Nat Cell Biol. 2021 Jan;23(1):99-108. do

Insert

dCas9, Suntag-P65-HSF1

Expression

Mammalian

Promoter

CAG

Available Since

May 5, 2021

Availability

Academic Institutions and Nonprofits only

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pAB2076 pAAV REPAIR.t1

Plasmid

#176323

Purpose

pAAV EFS-HIVNES-dCas13bt1- (GGS)2-huADAR2(E488Q)-3xHA BGHpolyA::U6-BpiI-Cas13bt1 DR (full REPAIR.t1 + crRNA expression for AAV production)

Depositor

Feng Zhang

Article

Kannan et al Nat Biotechnol. 2021 Aug 30. pii: 10.103

Inserts

human codon optimized Cas13bt1 (catalytically inactivated)
huADAR2dd(E488Q) (ADARB1 Human)
Cas13bt1 crRNA + BpiI cloning site

Use

AAV and CRISPR

Tags

3xHA and HIV NES

Expression

Mammalian

Mutation

E488Q, only the deaminase domain (aa 276-702 are …

Promoter

EFS (short EF1alpha) and hU6

Available Since

Oct. 14, 2021

Availability

Academic Institutions and Nonprofits only

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pAAV-Sox2-CreERT2

Plasmid

#135618

Purpose

Donor vector for genomic targeting of a P2A-CreERT2 cassette to the 3' end of the mouse Sox2 locus

Depositor

Lin He

Article

Chen et al Cell Rep. 2019 Jun 25;27(13):3780-3789.e

Insert

P2A-CreERT2 flanked by Sox2 homology arms (Sox2 Mouse)

Use

AAV, CRISPR, and Mouse Targeting

Tags

P2A-CreERT2

Available Since

April 21, 2020

Availability

Academic Institutions and Nonprofits only

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cycLuc_TEVS

Plasmid

#119207

Purpose

Circularly permutated firefly luciferase with TEV cleavage site (Luciferase reporter for detection of proteolytic activity)

Depositor

Roman Jerala

Article

Fink et al Nat Chem Biol. 2019 Feb;15(2):115-122. d

Insert

cyclic luciferase with TEVs cleavage site

Use

Luciferase

Tags

6xHis and AU1 tag

Expression

Mammalian

Promoter

CMV

Available Since

April 1, 2019

Availability

Academic Institutions and Nonprofits only

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AAV-FLEX-FLIM-AKAR

Plasmid

#60445

Purpose

Expresses the Fluorescence Lifetime Imaging Microscopy (FLIM)-compatible PKA activity sensor FLIM-AKAR in a Cre dependent manner in an AAV backbone

Depositor

Bernardo Sabatini

Article

Chen et al Front Pharmacol. 2014 Apr 2;5:56. doi: 1

Insert

FLIM-AKAR

Use

AAV and Cre/Lox

Expression

Mammalian

Promoter

CAG

Available Since

Oct. 22, 2014

Availability

Academic Institutions and Nonprofits only

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pAAV-nEF-Coff/Fon-Arch3.3-p2a-EYFP

Plasmid

#137150

Purpose

Intersectional viral expression of Arch3.3-EYFP in cells expressing Flp AND NOT Cre

Depositor

Karl Deisseroth, INTRSECT 2.0 Project

Article

Fenno et al Neuron. 2020 Jun 20. pii: S0896-6273(20)

Has Service

AAV8

Insert

Coff/Fon-Arch3.3-p2a-EYFP

Use

AAV, Cre/Lox, and Synthetic Biology ; Flp/frt

Mutation

n/a

Promoter

nEF

Available Since

June 25, 2020

Availability

Academic Institutions and Nonprofits only

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pAAV-hsyn-Jaws-GFP-ER2

Plasmid

#65016

Purpose

AAV-mediated expression of Jaws-ER2 under the human synapsin promoter

Depositor

Edward Boyden

Article

Chuong et al Nat Neurosci. 2014 Aug;17(8):1123-9. doi

Insert

Jaws-GFP-ER2

Use

AAV

Tags

ER2 and GFP

Expression

Mammalian

Mutation

K200R W214F

Promoter

human synapsin

Available Since

July 14, 2015

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn-GRAB_r5-HT1.0

Plasmid

#208719

Purpose

Expresses the red 5-HT sensor GRAB_r5-HT1.0 in neurons

Depositor

Yulong Li

Article

Deng et al Nat Methods. 2024 Apr;21(4):692-702. doi

Insert

Red fluorescent 5-HT sensor GRAB_r5-HT1.0

Use

AAV

Promoter

hSyn

Available Since

Oct. 24, 2023

Availability

Academic Institutions and Nonprofits only

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AAV-CAG-Cre

Plasmid

#196140

Purpose

AAV-mediated expression of Cre under the CAG promoter .

Depositor

Edward Boyden

Article

Chuong et al Nat Neurosci. 2014 Aug;17(8):1123-9. doi

Insert

Cre

Use

AAV

Expression

Mammalian

Mutation

codon diversified

Promoter

CAG

Available Since

June 30, 2023

Availability

Academic Institutions and Nonprofits only

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pYPQ-CBE-Cas9n-Act3.0

Plasmid

#178955

Purpose

It consists of a Cas9 nickase (D10A) fused with a cytidine deaminase (A3A/Y130F) and MS2-SunTag-activators (ScFv-sfGFP-2xTAD), enabling simultaneous C to T conversion and gene activation.

Depositor

Yiping Qi

Article

Pan et al Nat Plants. 2022 May;8(5):513-525. doi:

Insert

hA3A-zCas9-UGI-T2A-MS2-SunTag-rbcS-E9t-ter-ZmUbi-scFv-sfGFP-2xTAD-GB1-NOS-ter

Use

CRISPR

Expression

Plant

Available Since

May 23, 2022

Availability

Academic Institutions and Nonprofits only

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pJEP313-pAAV-CMV-SaCas9-DIO-pA

Plasmid

#113690

Purpose

A CMV driven inverted SaCas9. SaCas9 is floxed to render the system cre-dependent.

Depositor

Jonathan Ploski

Article

Kumar et al Front Mol Neurosci. 2018 Nov 13;11:413.

Insert

SaCas9 (NEWENTRY )

Use

AAV, CRISPR, and Cre/Lox

Tags

NLS

Promoter

Cytomegalo Virus(CMV)

Available Since

Feb. 14, 2019

Availability

Academic Institutions and Nonprofits only

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pAAV.pU1a-SpCas9

Plasmid

#121507

Purpose

Expresses codon-optimized SpCas9 in mammalian cells.

Depositor

Guangping Gao

Article

Wang et al Nat Biotechnol. 2018 Oct;36(9):839-842.

Insert

SpCas9

Use

AAV

Tags

HA

Expression

Mammalian

Promoter

U1a

Available Since

Feb. 5, 2019

Availability

Academic Institutions and Nonprofits only

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