We narrowed to 10,119 results for: try

1. TurboID-mNeongreen-3xFLAG fusion pos3

   Plasmid

   #172864

   Purpose

   C. elegans codon-optimized gateway entry vector expressing TurboID-mNeongreen fusion protein

   Depositor

   Mario de Bono

   Article

   Artan et al J Biol Chem. 2021 Sep;297(3):101094. doi

   Insert

   TurboID
   

   Use

   Unspecified

   Tags

   FLAG, mNeongreen, and myc

   Expression

   Mutation

   Promoter

   Available since

   Oct. 12, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. Pediculus humanus corporis PINK1 (115 - 575)

   Plasmid

   #110750

   Purpose

   Bacterial expression of PhPINK1 (biochemistry)

   Depositor

   David Komander

   Article

   Schubert et al Nature. 2017 Dec 7;552(7683):51-56. doi:

   Insert

   Phum_PHUM577390 (Phum_PHUM577390 Pediculus humanus corporis)
   

   Use

   Tags

   N-His6-GST-3C

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   June 14, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. p3E-FRT-KanR-FRT pA

   Plasmid

   #82601

   Purpose

   3' entry vector containing FRT-flanked kanamycin resistance cassette (KanR) with pA for FLP-induced kanamycin resistance, drug selection

   Depositor

   Kryn Stankunas

   Article

   Fowler et al PLoS One. 2016 Aug 8;11(8):e0159277. doi

   Insert

   FRT-Kan-FRT
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   None

   Available since

   Dec. 21, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pME-mVenus-CAAX

   Plasmid

   #75153

   Purpose

   Membrane targeted mVenus (yellow) fluorophore for use with 5’ promoters.

   Depositor

   Nicholas Cole

   Article

   Don et al Zebrafish. 2016 Sep 15.

   Insert

   mVenus-CAAX
   

   Use

   Middle entry vector for multisite gateway three f…

   Tags

   Expression

   Mutation

   Promoter

   n/a

   Available since

   Oct. 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pSR83

   Plasmid

   #90211

   Purpose

   Expresses the response regulator OmpR, and expresses mCherry from the Cph8-OmpR regulated PompF-146 promoter

   Depositor

   Jeffrey Tabor

   Article

   Olson et al Mol Syst Biol. 2017 Apr 24;13(4):926. do

   Inserts

   OmpR (ompR E. coli)
   mCherry
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   May 16, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pME-Gal80ts

   Plasmid

   #51619

   Purpose

   vertebrate expression of full-length temperature-sensitive Gal80 with stop

   Depositor

   Joshua Bonkowsky

   Article

   Fujimoto et al Dev Dyn. 2011 Oct;240(10):2324-34. doi:

   Insert

   Gal80ts (GAL80 Budding Yeast)
   

   Use

   Vertebrate expression

   Tags

   Expression

   Mutation

   temperature sensitive (G203V, G323R)*

   Promoter

   Available since

   March 27, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. hTLR3-pcDNA3

   Plasmid

   #32712

   Depositor

   Saumen Sarkar

   Article

   Zhu et al J Immunol. 2010 May 15;184(10):5768-76.

   Insert

   TLR3 (TLR3 Human)
   

   Use

   Tags

   FLAG and Preprototrypsin

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Nov. 29, 2011

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pBW103ParsR-Amp30C

   Plasmid

   #78638

   Purpose

   pBW100ParsR carrying 30hrpR-30hrpS-t-hrpL-30gfp-t

   Depositor

   Baojun Wang

   Article

   Wang et al Nucleic Acids Res. 2014 Aug;42(14):9484-

   Insert

   30hrpR-30hrpS-t-hrpL-30gfp-t
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   see insert

   Available since

   Aug. 3, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. SaCas9_sgRNA_expression_in_pBluescript

   Plasmid

   #122090

   Purpose

   U6 driven SaCas9 sgRNA expression vector for cloning own guides

   Depositor

   Scot Wolfe

   Article

   Bolukbasi et al Nat Commun. 2018 Nov 19;9(1):4856. doi:

   Insert

   SaCas9 tracr (NEWENTRY Synthetic, S. pyogenes)
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   April 18, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pYPQ131-tRNA2.0

    Plasmid

    #158393

    Purpose

    Golden Gate entry vector to express the 1st gRNA with tRNA2.0 scaffold (with four MS2 binding sites) without promoter

    Depositor

    Yiping Qi

    Article

    Pan et al Nat Plants. 2021 Jul;7(7):942-953. doi:

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Without promoter

    Available since

    June 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pSR59.4

    Plasmid

    #63175

    Purpose

    Expresses OmpR constitutively from PompB97 and sfGFP under the PompF146 promoter

    Depositor

    Jeffrey Tabor

    Article

    Schmidl et al ACS Synth Biol. 2014 Nov 21;3(11):820-31

    Inserts

    OmpR (ompR )
    sfGFP
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    April 15, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pSW454 - pUFO

    Plasmid

    #115978

    Purpose

    entry vector for GreenGate cloning method, promoter

    Depositor

    Jan Lohmann

    Article

    Schurholz et al Plant Physiol. 2018 Jul 19. pii: pp.18.0

    Insert

    pUFO
    

    Use

    Unspecified

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Nov. 2, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pYPQ152

    Plasmid

    #69303

    Purpose

    Gateway entry vector with pco-dCas9-VP64

    Depositor

    Yiping Qi

    Article

    Lowder et al Plant Physiol. 2015 Aug 21. pii: pp.0063

    Insert

    pco-dCas9-VP64 (Plant codon-optimized)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    VP64 activator fusion; NLS fusion

    Promoter

    Available since

    Sept. 18, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. plk1 T210D

    Plasmid

    #132964

    Purpose

    To study the processes involved in early mitotic events, mitotic progression, and control of re-entry after DNA damage

    Depositor

    Michael Yaffe

    Article

    Kishi et al Mol Cell Biol. 2009 Jun;29(11):3134-50.

    Insert

    polo-like kinase 1 (PLK1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    T210D

    Promoter

    Available since

    Feb. 17, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pBW101ParsR-gfp

    Plasmid

    #78636

    Purpose

    pSB3K3 carrying J101-rbs32-arsR-t-ParsR-30gfp-t

    Depositor

    Baojun Wang

    Article

    Wang et al Nucleic Acids Res. 2014 Aug;42(14):9484-

    Insert

    J101-rbs32-arsR-t-ParsR-30gfp-t
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    see insert

    Available since

    July 27, 2016

    Availability

    Academic Institutions and Nonprofits only

16. plk1 T210A

    Plasmid

    #132965

    Purpose

    To study the processes involved in early mitotic events, mitotic progression, and control of re-entry after DNA damage

    Depositor

    Michael Yaffe

    Article

    Kishi et al Mol Cell Biol. 2009 Jun;29(11):3134-50.

    Insert

    polo-like kinase 1 T210A (PLK1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    T210A

    Promoter

    Available since

    Dec. 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pUD-MSP1D1

    Plasmid

    #161608

    Purpose

    Bacterial expression of mutated membrane scaffold protein ("ultra-dark" MSP1D1) for Nanodisc formation

    Depositor

    Stephen Sligar

    Article

    McLean et al Anal Biochem. 2020 Oct 15;607:113860. do

    Insert

    UD-MSP1D1
    

    Use

    Tags

    7-His tag

    Expression

    Bacterial

    Mutation

    mutant of MSP1D1; all tryptophans and tyrosines w…

    Promoter

    T7

    Available since

    Nov. 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pET-28a (+)-Gal4-vp16

    Plasmid

    #171075

    Purpose

    Expresses Gal4-vp16 fusion protein in bacteria

    Depositor

    Joan Conaway

    Article

    He et al Proc Natl Acad Sci U S A. 2021 Sep 28;11

    Insert

    Gal4 DBD (NEWENTRY Budding Yeast)
    

    Use

    Tags

    His and VP16 AD (413-490)

    Expression

    Bacterial

    Mutation

    Promoter

    T7 promoter

    Available since

    July 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pYPQ153

    Plasmid

    #69304

    Purpose

    Gateway entry vector with pco-dCas9-3X(SRDX)

    Depositor

    Yiping Qi

    Article

    Lowder et al Plant Physiol. 2015 Aug 21. pii: pp.0063

    Insert

    pco-dCas9-3X(SRDX) (Plant codon-optimized)
    

    Use

    CRISPR

    Tags

    2XFLAG

    Expression

    Mutation

    SRDX (X3) repressor fusion; NLS fusion

    Promoter

    Available since

    Oct. 28, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pGR

    Plasmid

    #46002

    Purpose

    Reporter plasmid for measuring terminator strength by inserting terminator of interest between the GFP and RFP, expression driven by a single PBAD promoter inducible by arabinose

    Depositor

    Christopher Voigt

    Article

    Chen et al Nat Methods. 2013 Jun 2. doi: 10.1038/nm

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    PBAD

    Available since

    June 28, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge