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GC52 pEGFPN1 anti-Gephyrin nanobody

Plasmid

#135224

Purpose

Mammalian expression of anti-Gephyrin (Human) recombinant llama nanobody for use as an intrabody

Depositor

James Trimmer

Article

Dong et al Elife. 2019 Sep 30;8. pii: 48750. doi: 1

Insert

anti-Gephyrin (Human) recombinant llama nanobody GC52 (GPHN L. glama (llama))

Tags

6xHis-HA-EGFP

Expression

Mammalian

Available Since

Dec. 18, 2019

Availability

Academic Institutions and Nonprofits only

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pGTag-gal4-VP16-B-actin

Plasmid

#117817

Purpose

Donor for precise CRISPR directed genomic integration using the GeneWeld method

Depositor

Jeffrey Essner

Article

Wierson et al Elife. 2020 May 15;9. pii: 53968. doi: 1

Insert

gal4/VP16

Use

Synthetic Biology

Available Since

March 15, 2019

Availability

Academic Institutions and Nonprofits only

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pGL4.15_pSOX17

Plasmid

#195496

Purpose

Firefly luciferase promoter expression vector driven only by the promoter element of human SOX17

Depositor

Alexander Meissner

Article

Landshammer et al Elife. 2023 Jan 31;12:e83077. doi: 10.75

Insert

Promoter region of SOX17 (SOX17 Human)

Tags

luc2-hPEST

Expression

Mammalian

Available Since

Feb. 3, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-FGFR4-V5/HIS

Plasmid

#201109

Purpose

expression of human FGFR4 receptor tyrosine kinase in mammalian cells

Depositor

Pavel Krejčí

Article

Gudernova et al Elife. 2017 Feb 15;6. pii: e21536. doi:

Insert

human FGFR4 receptor tyrosine kinase, full length, wildtype (FGFR4 Human)

Tags

V5/His

Expression

Mammalian

Promoter

CMV

Available Since

May 26, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA5-T/O-Ds1-mCherry

Plasmid

#112855

Purpose

Dox inducible expression in mammalian cells of Ds1 protein fused to mCherry fluorescent protein

Depositor

David Sprinzak

Article

Loza et al Elife. 2017 Aug 18;6. pii: 24820. doi: 1

Insert

DCHS1 (DCHS1 Human)

Tags

mCherry

Expression

Mammalian

Promoter

doxycycline inducible promoter

Available Since

Aug. 15, 2018

Availability

Academic Institutions and Nonprofits only

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pCAG_Xph20-eGFP-CCR5TC

Plasmid

#135530

Purpose

Encodes a specific PSD-95 binder (Xph20) fused to eGFP, CCR5 left-handed zinc finger, and KRAB(A) transcriptional repressor domain

Depositor

Matthieu Sainlos

Article

Rimbault et al Elife. 2024 Jan 3;13:e69620. doi: 10.755

Insert

Xph20

Tags

eGFP

Expression

Mammalian

Promoter

CAG

Available Since

April 9, 2021

Availability

Academic Institutions and Nonprofits only

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pHDR_sox2HA_P2A-GFP-pA_HA_G2913A

Plasmid

#196191

Purpose

Tagging human SOX2 gene with P2A-EGFP at the C-terminus

Depositor

Jürgen Knoblich

Article

Sidhaye et al Elife. 2023 Mar 29;12:e85135. doi: 10.75

Insert

SOX2-HAL-P2A-EGFP-SOX2-HAR (SOX2 Human)

Use

Homology repair donor

Mutation

G2913A mutation to replace the guide cutting site…

Available Since

March 15, 2023

Availability

Academic Institutions and Nonprofits only

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pPB-cT3G-cERP2-NR4A1

Plasmid

#192925

Purpose

Barcoded piggybac transposon vector with Dox-inducible expression of NR4A1

Depositor

George Church

Article

Pierson Smela et al Elife. 2023 Feb 21;12:e83291. doi: 10.75

Insert

NR4A1 (NR4A1 Human)

Tags

HA

Expression

Mammalian

Promoter

TET3G-miniCMV

Available Since

Feb. 24, 2023

Availability

Academic Institutions and Nonprofits only

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pCAG_Xph20-mRuby2-CCR5TC

Plasmid

#135531

Purpose

Encodes a specific PSD-95 binder (Xph20) fused to mRuby2, CCR5 left-handed zinc finger, and KRAB(A) transcriptional repressor domain

Depositor

Matthieu Sainlos

Article

Rimbault et al Elife. 2024 Jan 3;13:e69620. doi: 10.755

Insert

Xph20

Tags

mRuby2

Expression

Mammalian

Promoter

CAG

Available Since

April 9, 2021

Availability

Academic Institutions and Nonprofits only

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pGEMHE-membrane-mEGFP

Plasmid

#105526

Purpose

T7 promotor drives in vitro mRNA transcription of a mEGFP-tagged membrane reporter

Depositor

Melina Schuh

Article

Clift et al Cell. 2017 Nov 13. pii: S0092-8674(17)31

Insert

GNAI2 (GNAI2 Human)

Use

In vitro transcription

Tags

mEGFP

Mutation

fragment encoding the plasma membrane targeting s…

Promoter

T7

Available Since

March 7, 2018

Availability

Academic Institutions and Nonprofits only

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