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pUPD_OsU3 (GB1184)

Plasmid

#68209

Purpose

Provides the O. sativa U3 RNA polIII promoter as a level 0 GoldenBraid part

Depositor

Diego Orzaez

Article

Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

Insert

OsU3 promoter

Use

CRISPR and Synthetic Biology

Mutation

BsaI and BsmBI sites removed

Available Since

March 16, 2016

Availability

Academic Institutions and Nonprofits only

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p6xUAS (GB0179)

Plasmid

#68182

Purpose

Provides 6xUAS (upstream activating sequence) as a level 0 GoldenBraid part

Depositor

Diego Orzaez

Article

Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

Insert

6xUAS

Use

Synthetic Biology

Mutation

BsaI and BsmBI sites removed

Available Since

March 16, 2016

Availability

Academic Institutions and Nonprofits only

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pCellFree_G03 TFDP3

Plasmid

#67083

Purpose

Standard for cell-free expression benchmarking.

Depositor

Kirill Alexandrov

Article

Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

Insert

TFDP3 (TFDP3 Human)

Use

Cell-free expression

Tags

EGFP and His

Promoter

T7

Available Since

Nov. 6, 2015

Availability

Academic Institutions and Nonprofits only
pT35S (GB0036)

Plasmid

#68187

Purpose

Provides the CaMV 35S terminator as a level 0 GoldenBraid part

Depositor

Diego Orzaez

Article

Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

Insert

CaMV 35S terminator

Use

Synthetic Biology

Mutation

BsaI and BsmBI sites removed

Available Since

Oct. 22, 2015

Availability

Academic Institutions and Nonprofits only

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pYFP (GB0053)

Plasmid

#68197

Purpose

Provides the CDS of the Yellow fluorescente protein as a level 0 GoldenBraid part

Depositor

Diego Orzaez

Article

Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

Insert

YFP coding region

Use

Synthetic Biology

Mutation

BsaI and BsmBI sites removed

Available Since

Oct. 19, 2015

Availability

Academic Institutions and Nonprofits only

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pCMB-TLEr

Plasmid

#61188

Purpose

Fluorescent reporter for transitory late endosome mCherry-MtRAB7A2 expressed under MtBCP1 promoter

Depositor

Maria Harrison

Article

Ivanov et al Plant J. 2014 Oct 20. doi: 10.1111/tpj.1

Insert

MtRAB7A2

Tags

mCherry

Expression

Plant

Promoter

MtBCP1

Available Since

Feb. 23, 2015

Availability

Academic Institutions and Nonprofits only

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EGFP-hPMCA4bct125

Plasmid

#47594

Depositor

Emanuel Strehler

Insert

PMCA4bct125 (ATP2B4 Human)

Tags

EGFP

Expression

Mammalian

Mutation

125 C terminal AA deleted

Promoter

CMV

Available Since

Aug. 27, 2013

Availability

Academic Institutions and Nonprofits only
TAL2418

Plasmid

#36819

Depositor

Keith Joung

Article

Reyon et al Nat Biotechnol. 2012 Apr 8. doi: 10.1038

Insert

PMS2-TALEN_Left (Pms2 Human)

Use

Mammalian, t7 for in vitro mrna synthesis

Tags

3X Flag and WT FOKI

Promoter

CMV

Available Since

July 16, 2012

Availability

Academic Institutions and Nonprofits only
TAL2419

Plasmid

#36820

Depositor

Keith Joung

Article

Reyon et al Nat Biotechnol. 2012 Apr 8. doi: 10.1038

Insert

PMS2-TALEN_Right (Pms2 Human)

Use

Mammalian, t7 for in vitro mrna synthesis

Tags

3X Flag and WT FOKI

Promoter

CMV

Available Since

June 26, 2012

Availability

Academic Institutions and Nonprofits only
pDONR223-CIB4

Plasmid

#23386

Depositor

William Hahn, David Root

Article

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

CIB4 (CIB4 Human)

Use

Gateway Cloning

Available Since

July 30, 2010

Availability

Academic Institutions and Nonprofits only
pCS2 Antivin

Plasmid

#24961

Depositor

Edward De Robertis

Article

Pera et al Int J Dev Biol. 2005 . 49(7):781-96.

Insert

Antivin (lefty-a Frog)

Expression

Mammalian

Available Since

June 18, 2010

Availability

Academic Institutions and Nonprofits only
pPIP1;4-NLS-3xmCitrine

Plasmid

#192524

Purpose

Fluorescent reporter for plant responses to bacterial infection

Depositor

Gitta Coaker

Article

Zhu et al Cell Rep. 2023 Jun 20;42(7):112676. doi:

Insert

PLASMA MEMBRANE INTRINSIC PROTEIN 1;4 (PIP1;4 Mustard Weed)

Tags

3xmCitrine and NLS

Expression

Plant

Promoter

PIP1;4

Available Since

June 23, 2023

Availability

Academic Institutions and Nonprofits only

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pET-28a(+)-His-mEGFP-iLID

Plasmid

#246940

Purpose

Expresses His-mEGFP-iLID in E.coli

Depositor

Makito Miyazaki

Article

Yamamoto et al Nat Commun. 2025 Aug 26;16(1):7583. doi:

Insert

iLID

Tags

His-tag, mEGFP

Expression

Bacterial

Available Since

April 15, 2026

Availability

Academic Institutions and Nonprofits only

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pX459-Dvl2 gRNA

Plasmid

#246556

Purpose

CRISPR vector co-expressing Cas9 and a mouse Dvl2 gRNA

Depositor

Mario Vallon

Article

Heiden et al J Biol Chem. 2025 Sep 4:110682. doi: 10.

Insert

Dvl2 gRNA (Dvl2 Mouse)

Use

CRISPR

Promoter

U6

Available Since

Dec. 2, 2025

Availability

Academic Institutions and Nonprofits only

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SIN40C.SFFV.dCas9-KRAB.P2A.mNeon

Plasmid

#170482

Purpose

Lentiviral dSpCas9-KRAB (CRISPRi) delivery, mNeon coexpression

Depositor

Dirk Heckl

Article

Ng et al iScience. 2023 Sep 7;26(10):107844. doi:

Inserts

dCas9-KRAB
SFFV
P2A-mNeonGreen

Use

Lentiviral

Tags

HA

Available Since

Aug. 5, 2025

Availability

Academic Institutions and Nonprofits only

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pCAG-sp-flag-hSema4D

Plasmid

#190656

Purpose

Expresses Human Sema4D with N terminal flag after signal peptide

Depositor

Andrew Newman, Victor Tarabykin

Article

Bessa et al Nat Commun. 2024 Aug 16;15(1):7059. doi:

Insert

Sema4D (SEMA4D Human)

Tags

flag

Expression

Mammalian

Available Since

July 23, 2025

Availability

Academic Institutions and Nonprofits only

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pCS2-zH2B-GFP

Plasmid

#226561

Purpose

Express H2B-GFP in zebrafish.

Depositor

Yong Zhang

Article

Wang et al Commun Biol. 2024 Oct 19;7(1):1354. doi:

Insert

h2b (h2bc1 Zebrafish)

Use

Synthetic Biology; Zebrafish expression

Tags

EGFP

Available Since

March 12, 2025

Availability

Academic Institutions and Nonprofits only

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pCAG-Cas13d-T2A-GFP(1-10)

Plasmid

#224566

Purpose

Ubiquitous expression plasmid, contains CAG promoter (CMV immediate early enhancer and chicken beta actin promoter), Cas13d, and split GFP(1-10) reporter.

Depositor

Erica Hutchins

Article

Kim et al Dev Biol. 2024 Nov 30:S0012-1606(24)0026

Insert

Cas13d-T2A-GFP(1-10)

Use

CRISPR

Tags

T2A-split GFP(1-10)

Available Since

Dec. 9, 2024

Availability

Academic Institutions and Nonprofits only

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pF(UG) hSyn PM-SYT7alpha deltaTMD

Plasmid

#179720

Purpose

Lentiviral expression of plasma membrane-targeted mouse Syt7

Depositor

Edwin Chapman

Article

Vevea et al Elife. 2021 Sep 20;10. pii: 67261. doi:

Insert

SYT7 (Syt7 Mouse)

Use

Lentiviral

Available Since

Sept. 3, 2024

Availability

Academic Institutions and Nonprofits only

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pF(UG) hSyn PP-HaloTag-SYT7alpha

Plasmid

#179714

Purpose

Lentiviral expression of mouse Syt7 with N terminal HaloTag

Depositor

Edwin Chapman

Article

Vevea et al Elife. 2021 Sep 20;10. pii: 67261. doi:

Insert

SYT7 (Syt7 Mouse)

Use

Lentiviral

Available Since

Sept. 3, 2024

Availability

Academic Institutions and Nonprofits only

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