We narrowed to 32,986 results for: Eng

1. BE3-hA3A-R128A

   Plasmid

   #132944

   Purpose

   Gene editing. See Gene/Insert section of the plasmid page for targeting sequence cloned into this plasmid.

   Depositor

   Hui Yang

   Article

   Zhou et al Nature. 2019 Jul;571(7764):275-278. doi:

   Insert

   BE3-hA3A(R128A)
   

   Use

   CRISPR; Base editor

   Expression

   Mammalian

   Mutation

   BE3-hA3A(R128A)

   Available Since

   Nov. 24, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. px330-CIB1-dCas9

   Plasmid

   #63665

   Purpose

   Mammalian gRNA expression vector also expressing CIB1-dCas9

   Depositor

   Dong Kong

   Article

   Chen et al Theranostics. 2018 Aug 7;8(16):4447-4461

   Type

   Empty backbone

   Use

   CRISPR

   Expression

   Mammalian

   Promoter

   U6 and CMV

   Available Since

   Sept. 13, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. pMTB2-cytoBirA-2A-mCherry_Ras

   Plasmid

   #80066

   Purpose

   Beta-actin2 promoter driving HA-tagged biotin ligase (BirA), a Thosea asigna virus peptide (2A), and membrane-tethered mCherry protein (membCherry); flanked by Tol2 sequences

   Depositor

   Tatjana Sauka-Spengler

   Article

   Trinh et al Cell Rep. 2017 Apr 11;19(2):425-440. doi

   Insert

   Biotin ligase (BirA), a Thosea asigna virus peptide (2A), and mCherry protein
   

   Use

   Zebrafish biotagging

   Tags

   3x HA

   Expression

   Bacterial

   Promoter

   Beta-actin2

   Available Since

   Oct. 11, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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4. pMTB2-NLS-BirA-2A-mCherry_Ras

   Plasmid

   #80067

   Purpose

   Beta-actin2 promoter driving HA-tagged biotin ligase (BirA) with NLS, a Thosea asigna virus peptide (2A), and membrane-tethered mCherry protein (membCherry); flanked by Tol2 sequences

   Depositor

   Tatjana Sauka-Spengler

   Article

   Trinh et al Cell Rep. 2017 Apr 11;19(2):425-440. doi

   Insert

   Biotin ligase (BirA) with nuclear localization signal (NLS), a Thosea asigna virus peptide (2A), and mCherry protein
   

   Use

   Zebrafish biotagging

   Tags

   3x HA

   Expression

   Bacterial

   Promoter

   Beta-actin2

   Available Since

   Oct. 11, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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5. pX330-NLC-DHFR-SpCas9

   Plasmid

   #124523

   Purpose

   Expresses SpCas9 fused to DHFR domains on both the N- and C- termini and an internal loop in mammalian cells

   Depositor

   Amit Choudhary

   Article

   Manna et al Angew Chem Int Ed Engl. 2019 May 6;58(19

   Insert

   NLC-DHFR-SpCas9
   

   Use

   AAV

   Tags

   Destabilized domain of E.coli dihydrofolate reduc…

   Expression

   Mammalian

   Promoter

   Cbh

   Available Since

   July 19, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. tdEos-Vimentin-7

   Plasmid

   #57688

   Purpose

   Localization: Intermediate Filaments, Excitation: 505 / 569, Emission: 516 / 581

   Depositor

   Michael Davidson

   Article

   Shtengel et al Proc Natl Acad Sci U S A. 2009 Mar 3;106

   Insert

   Vimentin (VIM Human)
   

   Tags

   tdEos

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Feb. 5, 2015

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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7. pGEM-cytoBirA-2A-mCherry-SV40pA-FKF

   Plasmid

   #79887

   Purpose

   Donor cassette containing HA-tagged biotin ligase (BirA), a Thosea asigna virus peptide (2A), mCherry protein and SV40 polyA tail, followed by FRT site-flanked Kanamycin selection gene

   Depositor

   Tatjana Sauka-Spengler

   Article

   Trinh et al Cell Rep. 2017 Apr 11;19(2):425-440. doi

   Insert

   HA-tagged BirA, 2A, mCherry protein and SV40 polyA, followed by FRT site-flanked Kanamycin selection gene
   

   Expression

   Bacterial

   Available Since

   Oct. 11, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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8. pMT-myl7-cytoBirA-2A-mCherry_Ras

   Plasmid

   #80060

   Purpose

   Myl7 promoter driving HA-tagged biotin ligase (BirA), a Thosea asigna virus peptide (2A), and membrane-tethered mCherry protein (membCherry); flanked by Tol2 sequences

   Depositor

   Tatjana Sauka-Spengler

   Article

   Trinh et al Cell Rep. 2017 Apr 11;19(2):425-440. doi

   Insert

   Biotin ligase (BirA), a Thosea asigna virus peptide (2A), and mCherry protein
   

   Use

   Zebrafish biotagging

   Tags

   3x HA

   Expression

   Bacterial

   Promoter

   Myl7 promoter

   Available Since

   Oct. 11, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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9. TMED3-hLOV-TEVcs-GAL4bd-VP64-V5

   Plasmid

   #170996

   Purpose

   HiLITR transcription factor with full-length TMED3 targeting sequence (ER/Golgi)

   Depositor

   Alice Ting

   Article

   Coukos et al eLife. 2021 Aug 20;10. pii: 69142. doi:

   Insert

   TMED3(FL)-NNES-hLOV-TEVcs(ENLYFQ/M)-GAL4bd-VP64-V5 (TMED3 Synthetic)
   

   Use

   Lentiviral and Synthetic Biology

   Tags

   V5

   Expression

   Mammalian

   Promoter

   Ef1-alpha

   Available Since

   Aug. 19, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pcDNA3.3-hALDOA D34S

    Plasmid

    #210810

    Purpose

    To reduce the catalytic constant (kcat) for conversion of FBP to dihydroxyacetone phosphate (DHAP) and glyceraldehyde-3-phosphate (G3P)

    Depositor

    Sheng-cai Lin

    Article

    Zhang et al Nature. 2017 Aug 3;548(7665):112-116. do

    Insert

    ALDOA (ALDOA Human)
    

    Tags

    HA

    Expression

    Mammalian

    Mutation

    residues 34 of ALDOA mutated to serine

    Promoter

    CMV

    Available Since

    Dec. 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. BE3-hA3A-Y130F

    Plasmid

    #132945

    Purpose

    Gene editing. See Gene/Insert section of plasmid page for targeting sequence cloned into this plasmid.

    Depositor

    Hui Yang

    Article

    Zhou et al Nature. 2019 Jul;571(7764):275-278. doi:

    Insert

    BE3-hA3A(Y130F)
    

    Use

    CRISPR; Base editor

    Expression

    Mammalian

    Mutation

    BE3-hA3A(Y130F)

    Available Since

    Nov. 24, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. pLV V5-TbID-emerin

    Plasmid

    #175099

    Purpose

    Lentiviral expression of V5-TbID-tagged mouse Emd

    Depositor

    Larry Gerace

    Article

    Cheng et al J Proteome Res. 2022 Sep 2;21(9):2197-22

    Insert

    Emerin (Emd Mouse)
    

    Use

    Lentiviral

    Tags

    V5-TbID

    Expression

    Mammalian

    Available Since

    Jan. 10, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pMT-zic2a-cytoBirA-2A-mCherry_Ras

    Plasmid

    #80064

    Purpose

    Zic2a promoter driving HA-tagged biotin ligase (BirA), a Thosea asigna virus peptide (2A), and membrane-tethered mCherry protein (membCherry); flanked by Tol2 sequences

    Depositor

    Tatjana Sauka-Spengler

    Article

    Trinh et al Cell Rep. 2017 Apr 11;19(2):425-440. doi

    Insert

    Biotin ligase (BirA), a Thosea asigna virus peptide (2A), and mCherry protein
    

    Use

    Zebrafish biotagging

    Tags

    3x HA

    Expression

    Bacterial

    Promoter

    Zic2a enhancer driving c-fos promoter

    Available Since

    Oct. 11, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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14. pMT-ubb-NLS-BirA-2a-mCherry

    Plasmid

    #79886

    Purpose

    Ubiquitin promoter driving HA-tagged biotin ligase (BirA) with nuclear localization signal (NLS), a Thosea asigna virus peptide (2A), and mCherry protein; flanked by Tol2 sequences

    Depositor

    Tatjana Sauka-Spengler

    Article

    Trinh et al Cell Rep. 2017 Apr 11;19(2):425-440. doi

    Insert

    Biotin ligase (BirA) with nuclear localization signal (NLS), a Thosea asigna virus peptide (2A), and mCherry protein
    

    Tags

    3x HA

    Expression

    Bacterial

    Promoter

    Ubiquitin

    Available Since

    Oct. 11, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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15. pMT-MCS-NLS-BirA-2A-mCherry_Ras

    Plasmid

    #80059

    Purpose

    MCS for cloning promoter to drive HA-tagged biotin ligase (BirA) with NLS, a Thosea asigna virus peptide (2A), and membrane-tethered mCherry protein (membCherry); flanked by Tol2 sequences

    Depositor

    Tatjana Sauka-Spengler

    Article

    Trinh et al Cell Rep. 2017 Apr 11;19(2):425-440. doi

    Insert

    Biotin ligase (BirA) with nuclear localization signal (NLS), a Thosea asigna virus peptide (2A), and mCherry protein
    

    Use

    Zebrafish biotagging

    Tags

    3x HA

    Expression

    Bacterial

    Available Since

    Oct. 11, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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16. Cas9_YTHDF2_sgRNA

    Plasmid

    #186673

    Purpose

    YTHDF2 sgRNA plasmid

    Depositor

    Astrid Haase

    Article

    Meng et al Nucleic Acids Res. 2022 May 27. pii: 659

    Insert

    YTHDF2 KO sgRNA Plasmid (YTHDF2 Human)
    

    Expression

    Mammalian

    Available Since

    Aug. 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCMV-GABARα1N-HA

    Plasmid

    #128110

    Purpose

    Express GABARα1 N terminal domain

    Depositor

    Huda Zoghbi

    Article

    Meng et al Elife. 2019 Sep 16;8. pii: 46773. doi: 1

    Insert

    Gabra1 (Gabra1 Mouse)
    

    Tags

    HA

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Sept. 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pGEM-BirA-2A-H2B-Dendra2-SV40pA-FKF

    Plasmid

    #119864

    Purpose

    Donor cassette containing HA-tagged biotin ligase (BirA), a Thosea asigna virus peptide (2A), H2B-tagged Dendra2 fluorophore and SV40 polyA tail, followed by FRT site-flanked Kanamycin selection gene

    Depositor

    Tatjana Sauka-Spengler

    Article

    Weinberger et al Dev Cell. 2020 Mar 9;52(5):574-590.e6. d

    Insert

    BirA, 2A, H2B-tagged Dendra2 and SV40 polyA, followed by FRT site-flanked Kanamycin selection gene
    

    Tags

    HA-tagged biotin ligase (BirA), Thosea asigna vir…

    Expression

    Bacterial

    Available Since

    March 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. Mouse GFP-PRMT7 LDIG(69-72)AAAA

    Plasmid

    #65044

    Purpose

    Expresses Catalytically inactive GFP-PRMT7 LDIG(69-72)AAAA mutant

    Depositor

    Steven Clarke

    Article

    Feng et al J Biol Chem. 2013 Dec 27;288(52):37010-2

    Insert

    PRMT7 (Prmt7 Mouse)
    

    Use

    Bacterial propagation

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    L69A D70A I71A G72A

    Promoter

    CMV

    Available Since

    July 8, 2015

    Availability

    Academic Institutions and Nonprofits only

20. tdEos-Keratin-17

    Plasmid

    #57629

    Purpose

    Note- This plasmid contains Keratin 18. Localization: Cytokeratin, Excitation: 505 / 569, Emission: 516 / 581

    Depositor

    Michael Davidson

    Article

    Shtengel et al Proc Natl Acad Sci U S A. 2009 Mar 3;106

    Insert

    Keratin (KRT18 Human)
    

    Tags

    tdEos

    Expression

    Mammalian

    Mutation

    V282L

    Promoter

    CMV

    Available Since

    Jan. 13, 2015

    Availability

    Industry, Academic Institutions, and Nonprofits