Skip to main content
Addgene

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

In Category

Filter By

We narrowed to 32,143 results for: Eng

Showing: 6341 - 6360 of 32143 results

  1. pGEX KGA F7

    Plasmid
    #227822
    Purpose
    To express in E. coli KGA F7 with 33 residues mutated to alanine at the interface for interacting with PDZD8
    Depositor
    Sheng-cai Lin
    Article
    Li et al Cell Res. 2024 Jun 19. doi: 10.1038/s414
    Insert
    KGA isoform of F7 (GLS Human)
    Use
    Tags
    GST
    Expression
    Bacterial
    Mutation
    P137, L139, E140, L142, Y145, G150, Q151, E152, K…
    Promoter
    Available since
    June 30, 2025
    Availability
    Academic Institutions and Nonprofits only
    227822-plasmid-map-sequence-id-477347

    Enlarge

  2. pBOBI C-MYC KGA F7

    Plasmid
    #227821
    Purpose
    To amplify the virus that expresses C-MYC KGA F7 with 33 residues mutated to alanine at the interface for interacting with PDZD8
    Depositor
    Sheng-cai Lin
    Article
    Li et al Cell Res. 2024 Jun 19. doi: 10.1038/s414
    Insert
    KGA isoform of F7 (GLS Human)
    Use
    Lentiviral
    Tags
    MYC
    Expression
    Mammalian
    Mutation
    P137, L139, E140, L142, Y145, G150, Q151, E152, K…
    Promoter
    Available since
    June 27, 2025
    Availability
    Academic Institutions and Nonprofits only
    227821-plasmid-map-sequence-id-477304

    Enlarge

  3. pVRC8400-SARS-CoV-2-S2P-BQ.1.1-AVI

    Plasmid
    #196621
    Purpose
    Expresses SARS-CoV-2 S2P protein from the BQ.1.1 variant with single chain Fc, HRV3C protease cleavage site, and AVI tag
    Depositor
    Peter Kwong
    Article
    Teng et al PLoS One. 2022 May 24;17(5):e0268767. do
    Insert
    SARS-CoV-2-S2P-BQ.1.1 (S )
    Use
    Tags
    AVI, HRV3C, and scFc
    Expression
    Mammalian
    Mutation
    T19I, L24-, P25-, P26-, A27S, H69-, V70-, G142D, …
    Promoter
    Available since
    Feb. 21, 2023
    Availability
    Academic Institutions and Nonprofits only
    196621-plasmid-map-sequence-id-385180

    Enlarge

  4. pVRC8400-SARS-CoV-2-S2P-BJ.1-AVI

    Plasmid
    #196620
    Purpose
    Expresses SARS-CoV-2 S2P protein from the BJ.1 variant with single chain Fc, HRV3C protease cleavage site, and AVI tag
    Depositor
    Peter Kwong
    Article
    Teng et al PLoS One. 2022 May 24;17(5):e0268767. do
    Insert
    SARS-CoV-2-S2P-BJ.1 (S )
    Use
    Tags
    AVI, HRV3C, and scFc
    Expression
    Mammalian
    Mutation
    T19I, L24-, P25-, P26-, A27S, V83A, G142D, Y144-,…
    Promoter
    Available since
    Feb. 21, 2023
    Availability
    Academic Institutions and Nonprofits only
    196620-plasmid-map-sequence-id-385179

    Enlarge

  5. pVRC8400-SARS-CoV-2-S2P-B.1.617-G142D-E154K-L452R-E484Q-D614G-P681R-Q1071H-H1101D-AVI

    Plasmid
    #176332
    Purpose
    Expresses SARS-CoV-2 S2P protein from the B.1.617 variant containing G142D-E154K-L452R-E484Q-D614G-P681R-Q1071H-H1101D mutations with single chain Fc, HRV3C protease cleavage site, and AVI tag
    Depositor
    Peter Kwong
    Article
    Teng et al PLoS One. 2022 May 24;17(5):e0268767. do
    Insert
    SARS-CoV-2-S2P-B.1.617-G142D-E154K-L452R-E484Q-D614G-P681R-Q1071H-H1101D (S )
    Use
    Tags
    AVI, HRV3C, and scFc
    Expression
    Mammalian
    Mutation
    G142D, E154K, L452R, E484Q, D614G, P681R, Q1071H,…
    Promoter
    Available since
    March 2, 2022
    Availability
    Academic Institutions and Nonprofits only
    176332-plasmid-map-sequence-id-347141

    Enlarge

  7. TRUPATH Triple GaZ

    Plasmid
    #196053
    Purpose
    Encodes a G alpha subunit (GNAZ) with RLuc8, a G gamma subunit (GNG1) with GFP2 and a G beta subunit (GNB3) as optimal components of a BRET2 biosensor for studying heterotrimeric G proteins
    Depositor
    Justin English
    Article
    Olsen et al Nat Chem Biol. 2020 May 4. pii: 10.1038/
    Inserts
    GAlphaz-RLuc8 (GNAZ Human)
    GGamma1-GFP2 (GNGT1 Human)
    GBeta3 (GNB3 Human)
    Use
    Luciferase
    Tags
    GFP2 and GSAG linker
    Expression
    Mammalian
    Mutation
    RLuc8 and flanking SGGGGS linkers have been inser…
    Promoter
    CMV
    Available since
    March 15, 2023
    Availability
    Academic Institutions and Nonprofits only
    196053-plasmid-map-sequence-id-387469

    Enlarge

  8. TRUPATH Triple Gai2

    Plasmid
    #196049
    Purpose
    Encodes a G alpha subunit (GNAl2) with RLuc8, a G gamma subunit (GNG8) with GFP2 and a G beta subunit (GNB3) as optimal components of a BRET2 biosensor for studying heterotrimeric G proteins
    Depositor
    Justin English
    Article
    Olsen et al Nat Chem Biol. 2020 May 4. pii: 10.1038/
    Inserts
    GAlphai2-RLuc8 (GNAI2 Human)
    GGamma8-GFP2 (GNG8 Human)
    GBeta3 (GNB3 Human)
    Use
    Luciferase
    Tags
    GFP2 and GSAG linker
    Expression
    Mammalian
    Mutation
    RLuc8 and flanking SGGGGS linkers have been inser…
    Promoter
    CMV
    Available since
    Feb. 27, 2023
    Availability
    Academic Institutions and Nonprofits only
    196049-plasmid-map-sequence-id-385863

    Enlarge

  9. TRUPATH Triple Ga11

    Plasmid
    #196059
    Purpose
    Encodes a G alpha subunit (GNA11) with RLuc8, a G gamma subunit (GNG13) with GFP2 and a G beta subunit (GNB3) as optimal components of a BRET2 biosensor for studying heterotrimeric G proteins
    Depositor
    Justin English
    Article
    Olsen et al Nat Chem Biol. 2020 May 4. pii: 10.1038/
    Inserts
    GAlpha11-RLuc8 (GNA11 Human)
    GGamma13-GFP2 (GNG13 Human)
    GBeta3 (GNB3 Human)
    Use
    Luciferase
    Tags
    GFP2 and GSAG linker
    Expression
    Mammalian
    Mutation
    RLuc8 and flanking SGGGGS linkers have been inser…
    Promoter
    CMV
    Available since
    Feb. 28, 2023
    Availability
    Academic Institutions and Nonprofits only
    196059-plasmid-map-sequence-id-386126

    Enlarge

  10. TRUPATH Triple Ga15

    Plasmid
    #196060
    Purpose
    Encodes a G alpha subunit (GNA15) with RLuc8, a G gamma subunit (GNG13) with GFP2 and a G beta subunit (GNB3) as optimal components of a BRET2 biosensor for studying heterotrimeric G proteins
    Depositor
    Justin English
    Article
    Olsen et al Nat Chem Biol. 2020 May 4. pii: 10.1038/
    Inserts
    GAlpha15-RLuc8 (GNA15 Human)
    GGamma13-GFP2 (GNG13 Human)
    GBeta3 (GNB3 Human)
    Use
    Luciferase
    Tags
    GFP2 and GSAG linker
    Expression
    Mammalian
    Mutation
    RLuc8 and flanking SGGGGS linkers have been inser…
    Promoter
    CMV
    Available since
    Feb. 27, 2023
    Availability
    Academic Institutions and Nonprofits only
    196060-plasmid-map-sequence-id-385870

    Enlarge

  11. TRUPATH Triple Gagust

    Plasmid
    #196054
    Purpose
    Encodes a G alpha subunit (GNAT3) with RLuc8, a G gamma subunit (GNG1) with GFP2 and a G beta subunit (GNB3) as optimal components of a BRET2 biosensor for studying heterotrimeric G proteins
    Depositor
    Justin English
    Article
    Olsen et al Nat Chem Biol. 2020 May 4. pii: 10.1038/
    Inserts
    GAlphaGustducin-RLuc8 (GNAT3 Human)
    GGamma1-GFP2 (GNGT1 Human)
    GBeta3 (GNB3 Human)
    Use
    Luciferase
    Tags
    GFP2 and GSAG linker
    Expression
    Mammalian
    Mutation
    RLuc8 and flanking SGGGGS linkers have been inser…
    Promoter
    CMV
    Available since
    May 15, 2023
    Availability
    Academic Institutions and Nonprofits only
    196054-plasmid-map-sequence-id-393090

    Enlarge

  12. pVRC8400-SARS-CoV-2-S2P-XBB.1.5-AVI

    Plasmid
    #198465
    Purpose
    Expresses SARS-CoV-2 S2P protein from the XBB.1.5 variant with single chain Fc, HRV3C protease cleavage site, and AVI tag
    Depositor
    Peter Kwong
    Article
    Teng et al PLoS One. 2022 May 24;17(5):e0268767. do
    Insert
    SARS-CoV-2-S2P-XBB.1.5 (S )
    Use
    Tags
    AVI, HRV3C, and scFc
    Expression
    Mammalian
    Mutation
    T19I, L24-, P25-, P26-, A27S, V83A, G142D, Y144-,…
    Promoter
    Available since
    March 21, 2023
    Availability
    Academic Institutions and Nonprofits only
    198465-plasmid-map-sequence-id-388112

    Enlarge

  13. AAV:ITR-U6-sgRNA(Kras)-U6-sgRNA(p53)-U6-sgRNA(Lkb1)-pEFS-Rluc-2A-Cre-shortPA-KrasG12D_HDRdonor-ITR (AAV-KPL)

    Plasmid
    #60224
    Purpose
    Expresses Cre recombinase and Renilla luciferase from the EFS promoter and three U6-driven sgRNAs targeting Kras, p53, and Lkb1. Contains KrasG12D HDR donor. AAV backbone.
    Depositor
    Feng Zhang
    Article
    Platt et al Cell. 2014 Sep 24. pii: S0092-8674(14)01
    Inserts
    sgRNA
    Renilla luciferase
    Cre recombinase
    KrasG12D HDR donor (Kras Mouse)
    Use
    AAV, CRISPR, Cre/Lox, and Mouse Targeting
    Tags
    Cre-HA and Rluc-P2A
    Expression
    Mammalian
    Mutation
    Promoter
    EFS, No promoter, and U6
    Available since
    Oct. 9, 2014
    Availability
    Academic Institutions and Nonprofits only
    60224-plasmid-map-sequence-id-418949

    Enlarge

  14. PermaPhos Kit

    Plasmid Kit
    #1000000226
    Purpose
    This kit is used for expressing recombinant proteins with site-specific, non-hydrolyzable phosphoserine (nhpSer) in an engineered self-sufficient E. coli genetic code expansion (GCE) expression system
    Depositor
    Ryan Mehl
    Available since
    Sept. 12, 2023
    Availability
    Academic Institutions and Nonprofits only

  15. NT-CRISPR plasmid collection

    Plasmid Kit
    #1000000189
    Purpose
    The NT-CRISPR plasmid collection contains all plasmids to perform genome engineering in Vibrio natriegens using the NT-CRISPR method.
    Depositor
    Anke Becker
    Available since
    May 9, 2022
    Availability
    Academic Institutions and Nonprofits only

  17. TALE Toolbox

    Plasmid Kit
    #1000000019
    Purpose
    Provides a complete set of plasmids for building your own TALE nucleases (TALENs) and transcription factors (TALE-TFs) for genome editing and transcription modulation applications.
    Depositor
    Feng Zhang
    Available since
    Dec. 16, 2011
    Availability
    Academic Institutions and Nonprofits only

  18. Rinehart & Söll Phosphoprotein Synthesis Kit

    Plasmid Kit
    #1000000020
    Purpose
    Allows Sep incorporation (directed by UAG) into a protein by an E. coli strain that harbors a Sep-accepting tRNA (tRNASep), its cognate Sep–tRNA synthetase (SepRS), and an engineered EF-Tu (EF-Sep).
    Depositor
    Dieter Söll, Jesse Rinehart
    Available since
    March 1, 2012
    Availability
    Academic Institutions and Nonprofits only

  20. OVC Extended Collection

    Plasmid Kit
    #1000000165
    Purpose
    Four plasmids containing the cloning cassette, eight plasmids containing LSDs engineered to heterodimerize and their binding partners, and five plasmids with different light-activated Opto-caspase 9.
    Depositor
    Harald Janovjak
    Available since
    Oct. 9, 2020
    Availability
    Academic Institutions and Nonprofits only
Showing: 6341 - 6360 of 32143 results