We narrowed to 15,981 results for: LEA

1. pLenti CMV SNAP MDC1

   Plasmid

   #227375

   Purpose

   SNAP–MDC1 expression

   Depositor

   David Pellman

   Article

   Papathanasiou et al Nature. 2023 Jul;619(7968):184-192. doi:

   Insert

   MDC1, mediator of DNA damage checkpoint 1 (MDC1 Human)
   

   Use

   Lentiviral

   Tags

   SNAP

   Expression

   Mammalian

   Available Since

   Oct. 24, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. GST PTBP1 isoform 4

   Plasmid

   #108594

   Purpose

   Recombinant GST tagged protein production

   Depositor

   Chris Smith

   Article

   Coelho et al EMBO J. 2015 Mar 4;34(5):653-68. doi: 10

   Insert

   PTBP1 isoform 4 (PTBP1 Human)
   

   Tags

   GST

   Expression

   Bacterial

   Mutation

   isoform 4 (isoform a)

   Promoter

   tac

   Available Since

   Dec. 12, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCMV-ABE7.9

   Plasmid

   #102918

   Purpose

   expresses ABE7.9 in mammalian cells

   Depositor

   David Liu

   Article

   Gaudelli et al Nature. 2017 Nov 23;551(7681):464-471. d

   Insert

   ABE7.9
   

   Tags

   NLS

   Expression

   Mammalian

   Mutation

   see manuscript

   Promoter

   CMV

   Available Since

   Nov. 27, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. pGL3-NurRE3

   Plasmid

   #208253

   Purpose

   pGL3 promoter luciferase reporter vector containing 3 copies of the NurRE POMC promoter DNA response element (5′-GATCGTGATATTTACCTCCAAATGCCA- 3′) used for luciferase reporter assays in mammalian cells

   Depositor

   Douglas Kojetin

   Article

   Munoz-Tello et al J Med Chem. 2020 Dec 24;63(24):15639-156

   Insert

   3X Nur response element LUC
   

   Tags

   No

   Expression

   Mammalian

   Promoter

   SV40 early promoter

   Available Since

   Feb. 28, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pAAV-EF1a-vCre

   Plasmid

   #55638

   Purpose

   EF1a-driven expression of VCre in Mammalian Cells

   Depositor

   Karl Deisseroth

   Article

   Fenno et al Nat Methods. 2014 Jul;11(7):763-72. doi:

   Has Service

   AAV Retrograde and AAV8

   Insert

   vCre
   

   Use

   AAV

   Expression

   Mammalian

   Promoter

   Ef1a

   Available Since

   July 10, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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6. Cre-p2a-tdTomato

   Plasmid

   #176837

   Purpose

   Express Cre in mammalian cells and enable FACS sorting Cre expressing cells by tdTomato expression

   Depositor

   Rudolf Jaenisch

   Article

   Ma et al Cell Stem Cell. 2022 Apr 13. pii: S1934-

   Insert

   Cre-P2A-tdTomato
   

   Expression

   Mammalian

   Promoter

   CAGGS

   Available Since

   Nov. 2, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pBABE-Flag-Cdk9-D167N-IRES-eGFP

   Plasmid

   #28098

   Depositor

   Andrew Rice

   Article

   Dow et al J Cell Physiol. 2010 Jul . 224(1):84-93.

   Insert

   Cdk9 (CDK9 Human)
   

   Use

   Retroviral

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Contains D167N mutation in Cdk9

   Available Since

   April 12, 2011

   Availability

   Academic Institutions and Nonprofits only

8. pY002 (pFnCpf1_min)

   Plasmid

   #69975

   Purpose

   Expresses FnCpf1 and spacers 1-4 of CRISPR array under artificial promoter. Cas genes are removed.

   Depositor

   Feng Zhang

   Article

   Zetsche et al Cell. 2015 Sep 23. pii: S0092-8674(15)01

   Insert

   FnCpf1 locus without Cas genes nd artificial promoter
   

   Use

   CRISPR

   Expression

   Bacterial

   Available Since

   Oct. 8, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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9. pBABE-Flag-Cdk9-T186A-IRES-eGFP

   Plasmid

   #28097

   Depositor

   Andrew Rice

   Article

   Dow et al J Cell Physiol. 2010 Jul . 224(1):84-93.

   Insert

   Cdk9 (CDK9 Human)
   

   Use

   Retroviral

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Contains T186A mutation in Cdk9

   Available Since

   April 15, 2011

   Availability

   Academic Institutions and Nonprofits only

10. pRMC2-sec:msfGFP

    Plasmid

    #194914

    Purpose

    Tetracycline inducible expression of msfGFP fused to Sec signal peptide in Staphylococci

    Depositor

    Rikke Meyer

    Article

    Evans et al Microb Cell. 2023 Jun 28;10(7):145-156.

    Insert

    Shine Dalgarno sequence followed by Sec signal peptide fused to monomeric superfolder GFP
    

    Use

    Tetracycline-inducible expression vector for stap…

    Expression

    Bacterial

    Available Since

    July 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. AAV-SYP1-miniSOG-citrine

    Plasmid

    #50971

    Purpose

    AAV2 transfer vector containing the InSynC (SYP1-miniSOG-citrine) construct

    Depositor

    Roger Tsien

    Article

    Lin et al Neuron. 2013 Jul 24;79(2):241-53. doi: 1

    Insert

    Synaptophysin-miniSOG-citrine (Syp Rat)
    

    Use

    AAV

    Tags

    citrine and miniSOG

    Promoter

    human synapsin promoter

    Available Since

    Feb. 5, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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12. pSI-Target-AID-NG

    Plasmid

    #119861

    Purpose

    Expresses Target-AID-NG (NLS-nSpCas9-NG-NLS-SH3-3xFLAG-PmCDA1-NLS-UGI) in mammalian cells.

    Depositor

    Osamu Nureki

    Article

    Nishimasu et al Science. 2018 Aug 30. pii: science.aas91

    Insert

    nSpCas9-NG (D10A/L1111R/D1135V/G1218R/E1219F/A1322R/R1335V/T1337R)
    

    Expression

    Mammalian

    Available Since

    Dec. 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. SpCas9-NG-APOBEC-nCas9-Ung

    Plasmid

    #198539

    Purpose

    Expresses human codon-optimized SpCas9-NG-APOBEC-nCas9-Ung and blasticidin resistance: EFS promoter-SpCas9-NG-APOBEC-nCas9-Ung-NLS-FLAG-P2A-BSD

    Depositor

    Hyongbum Kim

    Article

    Kim et al Nat Biotechnol. 2024 Mar;42(3):484-497.

    Insert

    SpCas9-NG-APOBEC-nCas9-Ung
    

    Use

    CRISPR and Lentiviral

    Tags

    BSD, FLAG, and NLS

    Expression

    Mammalian

    Promoter

    EFS

    Available Since

    Nov. 5, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. pAAV CMV-DIO-(mCherry-U6)-shRNA(scrambled)

    Plasmid

    #214733

    Purpose

    Expresses mCherry protein in infected and cre positive cells

    Depositor

    Félix Leroy

    Article

    de Leon Reyes et al Cell. 2023 Sep 14;186(19):4152-4171.e31.

    Insert

    anti-Crh shRNA (scrambled) / mCherry
    

    Use

    AAV

    Available Since

    May 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. pLVH-EF1a-GFP-P2A-SOX2

    Plasmid

    #130693

    Purpose

    Lentiviral plasmids encoding human SOX2 with co-expressoin of GFP mediated by P2A.

    Depositor

    Kejin Hu

    Article

    Shao et al Nat Commun. 2016 Mar 7;7:10869. doi: 10.

    Insert

    human SOX2 (SOX2 Human)
    

    Use

    Lentiviral

    Available Since

    Aug. 22, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCMV_eA3A-NG-nCas9

    Plasmid

    #163536

    Purpose

    Mammalian CG-to-GC base editing

    Depositor

    David Liu

    Article

    Koblan et al Nat Biotechnol. 2021 Jun 28. pii: 10.103

    Insert

    eA3A-NG-nCas9
    

    Use

    CRISPR

    Expression

    Mammalian

    Mutation

    nCas9 (D10A)NG (L111R, D1135V, G1218R, E1219F, A1…

    Available Since

    Dec. 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. pAAV CMV-DIO-(mCherry-U6)-shRNA(anti-Crh)

    Plasmid

    #214732

    Purpose

    Expresses shRNA against CRH, marked with mCherry, in infected and cre positive cells

    Depositor

    Félix Leroy

    Article

    de Leon Reyes et al Cell. 2023 Sep 14;186(19):4152-4171.e31.

    Insert

    anti-Crh shRNA / mCherry
    

    Use

    AAV

    Available Since

    July 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCDNA3.1-ADGRL3-Flag

    Plasmid

    #201218

    Purpose

    pCDNA3.1 with kozak (GCC), ADGRL3 and C-terminal Flag

    Depositor

    Jonathan Javitch

    Article

    Perry-Hauser et al J Biol Chem. 2022 Dec;298(12):102594. do

    Insert

    ADGRL3 (Adgrl3 Mouse)
    

    Tags

    Flag

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    May 9, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. pFBD-Akt13C(S473D)

    Plasmid

    #86577

    Purpose

    Expresses cleavable human full-length Akt1(S473D) phosphomimetic

    Depositor

    Ivan Yudushkin, Thomas Leonard

    Article

    Ebner et al Mol Cell. 2017 Feb 2;65(3):416-431.e6. d

    Insert

    Akt1 (AKT1 Human)
    

    Tags

    10xHis

    Expression

    Insect

    Mutation

    3C cleavage site inserted at pos. 130; S473D phos…

    Available Since

    April 4, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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20. pGEX-5X-3-SARS-CoV-2-3CL

    Plasmid

    #168457

    Purpose

    Bacterial codon optimized expression vector for SARS-CoV-2 3CL protease.

    Depositor

    Alejandro Chavez, David Ho, Sho Iketani

    Article

    Iketani et al Nat Commun. 2021 Apr 1;12(1):2016. doi:

    Insert

    SARS-CoV-2 3CL protease (ORF1ab Human)
    

    Tags

    GST

    Expression

    Bacterial

    Promoter

    tac

    Available Since

    March 30, 2021

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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