We narrowed to 25,810 results for: LEA

1. pKAR2-Br512_Mut235

   Plasmid

   #179278

   Purpose

   Expresses Br512_Mut235 polymerase in E.coli

   Depositor

   Andrew Ellington

   Article

   Paik et al Biochemistry. 2021 Nov 11. doi: 10.1021/

   Insert

   Engineered Bst DNA Polymerase I Large Fragment
   

   Tags

   8x His tag; Villin Headpiece 47 amino acid (HP47)…

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   May 16, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. MARIO/ pcDNA3

   Plasmid

   #124838

   Purpose

   mammalian expression of MARIO, a genetically-encoded fluorescent Mg2+ sensor

   Depositor

   Takeharu Nagai

   Article

   Maeshima et al Curr Biol. 2018 Feb 5;28(3):444-451.e6.

   Insert

   MARIO
   

   Tags

   No tag

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   May 8, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pcDNAKv10.1mVenus

   Plasmid

   #85705

   Purpose

   Kv10.1 fused mVenus

   Depositor

   Luis Pardo

   Article

   Chen et al PLoS One. 2011 May 3;6(5):e19257. doi: 1

   Insert

   KCNH1 (KCNH1 Human)
   

   Tags

   mVenus

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Dec. 2, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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4. pEF Synaptophysin-mRuby

   Plasmid

   #188980

   Purpose

   Synaptic buton marker

   Depositor

   Edwin Chapman

   Article

   Courtney et al Neuron. 2018 Jun 6;98(5):977-991.e5. doi

   Insert

   Synaptophysin (Syp Synthetic, Rat)
   

   Expression

   Mammalian

   Available Since

   Oct. 21, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCW57-MCS1-P2A-MCS2 (Hygro)

   Plasmid

   #80922

   Purpose

   All-in-one doxycycline inducible lentiviral vector for expression of one or two genes using the P2A self-cleaving peptide.

   Depositor

   Adam Karpf

   Article

   Barger et al Cancers (Basel). 2019 Feb 21;11(2). pii:

   Has Service

   Cloning Grade DNA

   Type

   Empty backbone

   Use

   Lentiviral; Doxycycline inducible; p2a self cleav…

   Expression

   Mammalian

   Promoter

   TRE

   Available Since

   Sept. 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. CRY2olig-PR

   Plasmid

   #218917

   Purpose

   Expression of CRY2olig-mCherry fused to a codon-optimised poly-PR sequence

   Depositor

   Tatyana Shelkovnikova

   Article

   Hodgson et al iScience. 2024 Sep 14;27(10):110937. doi

   Insert

   CRY2olig-mCherry tagged poly-PR
   

   Expression

   Mammalian

   Mutation

   wild-type

   Promoter

   CMV

   Available Since

   Aug. 13, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. CRY2olig-GR

   Plasmid

   #218919

   Purpose

   Expression of CRY2olig-mCherry fused to a codon-optimised poly-GR sequence

   Depositor

   Tatyana Shelkovnikova

   Article

   Hodgson et al iScience. 2024 Sep 14;27(10):110937. doi

   Insert

   CRY2olig-mCherry tagged poly-GR
   

   Expression

   Mammalian

   Mutation

   wild-type

   Promoter

   CMV

   Available Since

   Aug. 13, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. CRY2olig-GP

   Plasmid

   #218918

   Purpose

   Expression of CRY2olig-mCherry fused to a codon-optimised poly-GP sequence

   Depositor

   Tatyana Shelkovnikova

   Article

   Hodgson et al iScience. 2024 Sep 14;27(10):110937. doi

   Insert

   CRY2olig-mCherry tagged poly-GP
   

   Expression

   Mammalian

   Mutation

   wild-type

   Promoter

   CMV

   Available Since

   Aug. 13, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pTRIPZ shNS

   Plasmid

   #127696

   Purpose

   Doxycyclin inducible pTRIPZ lentivirus vector containing a non-silencing sequence for both human and mouse

   Depositor

   Sandra Demaria

   Article

   Vanpouille-Box et al Nat Commun. 2017 Jun 9;8:15618. doi: 10.

   Insert

   shNS
   

   Use

   Lentiviral

   Expression

   Mammalian

   Available Since

   Nov. 1, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. TR-Csy4-R115A/R119A

    Plasmid

    #80696

    Purpose

    Expresses Csy4 with R115A and R119A mutations from the CBA promoter. Cloned into an Adeno-Associated Virus backbone for packaging into AAV.

    Depositor

    Aravind Asokan

    Article

    Borchardt et al RNA. 2015 Nov;21(11):1921-30. doi: 10.12

    Insert

    Csy4-R115A/R119A
    

    Use

    AAV

    Expression

    Mammalian

    Mutation

    Arg115 and Arg119 both mutated to Ala

    Promoter

    CBA

    Available Since

    Aug. 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCMVTNT-T7-KPNA6

    Plasmid

    #26682

    Depositor

    Bryce Paschal

    Article

    Kelley et al BMC Cell Biol. 2010 Aug 11;11:63. doi: 1

    Insert

    Karyopherin Alpha 6 (KPNA6 Human)
    

    Tags

    T7

    Expression

    Mammalian

    Available Since

    Nov. 10, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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12. UBXD1-GFP

    Plasmid

    #86464

    Purpose

    Expression of human UBXD1 with C-terminal GFP tag

    Depositor

    Hemmo Meyer

    Article

    Papadopoulos et al EMBO J. 2016 Oct 17. pii: e201695148.

    Insert

    UBXD1 (UBXN6 Human)
    

    Tags

    GFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Jan. 31, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. pEGFP-C1-ER alpha

    Plasmid

    #28230

    Purpose

    Mammalian expression of Estrogen Receptor alpha fused to EGFP

    Depositor

    Michael Mancini

    Article

    Stenoien et al Mol Endocrinol. 2000 Apr . 14(4):518-34.

    Insert

    Estrogen Receptor alpha (ESR1 Human)
    

    Tags

    EGFP

    Expression

    Mammalian

    Available Since

    April 7, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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14. ER-GCaMP6-150

    Plasmid

    #86918

    Purpose

    Fluorescent reporter for ER calcium signaling

    Depositor

    Timothy Ryan

    Article

    de Juan-Sanz et al Neuron. 2017 Jan 30. pii: S0896-6273(17)

    Insert

    ER-GCaMP6-150
    

    Use

    Lentiviral

    Expression

    Mammalian

    Mutation

    K78H, T302L, R303P, A317E, D324G, D360G, D380Y, T…

    Promoter

    CAMKII

    Available Since

    March 15, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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15. ER-GCaMP6-210

    Plasmid

    #86919

    Purpose

    Fluorescent reporter for ER calcium signaling

    Depositor

    Timothy Ryan

    Article

    de Juan-Sanz et al Neuron. 2017 Jan 30. pii: S0896-6273(17)

    Insert

    ER-GCaMP6-210
    

    Use

    Lentiviral

    Expression

    Mammalian

    Mutation

    K78H, T302L, R303P, A317E, D324G, D360G, D380Y, T…

    Promoter

    CAMKII

    Available Since

    March 15, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. pRSFDuet-1_VNp6-mNeongreen

    Plasmid

    #182388

    Purpose

    Bacterial expression of Vesicle Nucleating peptide6-mNeongreen fusion

    Depositor

    Dan Mulvihill

    Article

    Eastwood et al Cell Rep Methods. 2023 Feb 2;3(2):100396

    Insert

    VNp6-mNeongreen-His
    

    Tags

    Vesicle Nucleating peptide (VNp)

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Feb. 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. pETDuet-LahT150

    Plasmid

    #166785

    Purpose

    Cleavage of double glycine motif in peptides to remove the leader sequence

    Depositor

    Wilfred van der Donk

    Article

    Bobeica et al Elife. 2019 Jan 14;8. pii: 42305. doi: 1

    Insert

    LahT150
    

    Tags

    His6

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    April 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. pLVX Nup62Fv2GFP

    Plasmid

    #139683

    Purpose

    Nup62 dimerization construct fused to 2 copies of GFP under an CMV promoter

    Depositor

    Edward Campbell

    Article

    Dharan et al Nat Microbiol. 2020 Jun 1. pii: 10.1038/

    Insert

    Nup62 (NUP62 Human)
    

    Use

    Lentiviral

    Tags

    2 copies of GFP and Dimerization domain FKBP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    June 1, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. AAV sgRNA Expression Plasmid

    Plasmid

    #174540

    Purpose

    Contains restriction sites to clone in U6 promoter and sgRNA oligo. CMV-driven mCherry fluorescent marker.

    Depositor

    Ormond MacDougald

    Article

    Romanelli et al J Biol Chem. 2021 Nov 11:101402. doi: 10

    Type

    Empty backbone

    Use

    AAV

    Tags

    None

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Nov. 16, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. CuO-LacI-NLS–SNAPf

    Plasmid

    #227368

    Purpose

    Lentiviral expression of LacI–SNAP

    Depositor

    David Pellman

    Article

    Papathanasiou et al Nature. 2023 Jul;619(7968):184-192. doi:

    Insert

    LacI NLS SNAPf
    

    Use

    Lentiviral

    Expression

    Mammalian

    Available Since

    Oct. 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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