We narrowed to 12,396 results for: cel.2;

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pC1-SypHer3s

Plasmid

#108118

Purpose

Ratiometric pH probe with enhanced brightness for mammalian cells expression.

Depositor

Vsevolod Belousov

Article

Ermakova et al Chem Commun (Camb). 2018 Mar 2. doi: 10.

Insert

SypHer3s

Tags

ratiometric YFP

Expression

Mammalian

Promoter

CMV

Available Since

April 3, 2018

Availability

Academic Institutions and Nonprofits only

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pEGFP‐RNASEH2A

Plasmid

#108700

Purpose

For expression of N-terminally eGFP-tagged human RNASEH2A in mammalian cells

Depositor

Andrew Jackson, Martin Reijns

Article

Bubeck et al Nucleic Acids Res. 2011 May;39(9):3652-6

Insert

RNASEH2A (RNASEH2A Human)

Tags

GFP

Expression

Mammalian

Promoter

CMV

Available Since

April 24, 2018

Availability

Academic Institutions and Nonprofits only

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pEGFP‐RNASEH2B

Plasmid

#108697

Purpose

For expression of N-terminally eGFP-tagged human RNASEH2B in mammalian cells

Depositor

Andrew Jackson, Martin Reijns

Article

Bubeck et al Nucleic Acids Res. 2011 May;39(9):3652-6

Insert

RNASEH2B (RNASEH2B Human)

Tags

GFP

Expression

Mammalian

Promoter

CMV

Available Since

April 24, 2018

Availability

Academic Institutions and Nonprofits only

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pCMV-SpRY-HF1-ABE8e

Plasmid

#185670

Purpose

Expresses SpRY-HF1-ABE8e in mammalian cells

Depositor

David Liu

Article

Huang et al Nat Biotechnol. 2022 Sep 8. pii: 10.1038

Insert

ecTadA(8e)-nSpRY-HF1

Use

CRISPR

Expression

Mammalian

Mutation

SpRY-HF1 D10A

Available Since

Sept. 16, 2022

Availability

Academic Institutions and Nonprofits only

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pCMV-eNme2-C.NR

Plasmid

#185672

Purpose

Expresses nuclease active eNme2-C.NR in mammalian cells

Depositor

David Liu

Article

Huang et al Nat Biotechnol. 2022 Sep 8. pii: 10.1038

Insert

eNme2-C.NR nuclease

Use

CRISPR

Expression

Mammalian

Mutation

eNme2-C S6P/G33E/A520E/S646R/V696F/R711G/V758I/Y7…

Available Since

Sept. 16, 2022

Availability

Academic Institutions and Nonprofits only

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phMYT1L-N106

Plasmid

#60861

Purpose

Expresses MYT1L under the EF1a promoter

Depositor

Andrew Yoo

Article

Victor et al Neuron. 2014 Oct 22;84(2):311-23. doi: 1

Insert

MYT1L (MYT1L Human)

Use

Lentiviral

Expression

Mammalian

Promoter

EF1a

Available Since

Dec. 5, 2014

Availability

Academic Institutions and Nonprofits only

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pEGFP‐RNASEH2C

Plasmid

#108698

Purpose

For expression of N-terminally eGFP-tagged human RNASEH2C in mammalian cells

Depositor

Andrew Jackson, Martin Reijns

Article

Bubeck et al Nucleic Acids Res. 2011 May;39(9):3652-6

Insert

RNASEH2C (RNASEH2C Human)

Tags

GFP

Expression

Mammalian

Promoter

CMV

Available Since

April 24, 2018

Availability

Academic Institutions and Nonprofits only

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CaM1234(D20A/D56AD93A/D129A)/pIRES2-eGFP

Plasmid

#111518

Purpose

Electrophysiology in HEK cell

Depositor

Dan Minor

Article

Chang et al Neuron. 2018 Feb 21;97(4):836-852.e6. do

Insert

calmodulin [Xenopus laevis] (calm2 Frog)

Expression

Mammalian

Mutation

D20A/D56AD93A/D129A

Available Since

Sept. 21, 2018

Availability

Academic Institutions and Nonprofits only

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pQC.Flip-nluc(LgBiT1-8)CP[Casp3A]-Fluc

Plasmid

#200123

Purpose

Retroviral expression of Flip-nluc(LgBiT1-8)CP[Casp3A] protease reporter and P2A firefly luciferase in mammalian cells

Depositor

Eiji Morita

Article

Arakawa et al Sci Rep. 2023 Jan 31;13(1):1753. doi: 10

Insert

Flip-nluc(LgBiT1-8)CP[Casp3A] P2A firefly luciferase

Use

Retroviral

Expression

Mammalian

Promoter

CMV

Available Since

May 11, 2023

Availability

Academic Institutions and Nonprofits only

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VAMP2-12aa-mCit

Plasmid

#53237

Purpose

Fluorescent probe: SNAP-25B, C-terminally-tagged with monomeric Citrine, connected with a 12 amino acid linker. Expressed in mammalian cells, used with Stx1a-12aa-mCer to measure FRET and dispersion.

Depositor

Robert Zucker

Article

Degtyar et al J Neurosci. 2013 Mar 27;33(13):5507-23.

Insert

VAMP2 (Vamp2 Rat)

Tags

12aa-linker and mCitrine

Expression

Mammalian

Available Since

June 9, 2014

Availability

Academic Institutions and Nonprofits only
pRRL-5xTetO-pEF-H2B-Citrine-SV40

Plasmid

#186966

Purpose

Lentiviral vector encoding fluorescent reporter (5xTetO-pEF-H2B-Citrine-SV40) for expression in mammalian cells.

Depositor

Brian Liau

Article

Lue et al Nat Chem Biol. 2023 Feb;19(2):176-186. d

Insert

5xTetO-pEF-H2B-Citrine-SV40

Use

Lentiviral

Expression

Mammalian

Promoter

5xTetO-pEF-1alpha

Available Since

Oct. 14, 2022

Availability

Academic Institutions and Nonprofits only

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CaM34(D93A/D129A)/pIRES2-eGFP

Plasmid

#111517

Purpose

Electrophysiology in HEK cell

Depositor

Dan Minor

Article

Chang et al Neuron. 2018 Feb 21;97(4):836-852.e6. do

Insert

calmodulin [Xenopus laevis] (calm2 Frog)

Expression

Mammalian

Mutation

D93A/D129A

Available Since

Sept. 21, 2018

Availability

Academic Institutions and Nonprofits only

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CaM12(D20A/D56A)/pIRES2-eGFP

Plasmid

#111512

Purpose

Electrophysiology in HEK cell

Depositor

Dan Minor

Article

Chang et al Neuron. 2018 Feb 21;97(4):836-852.e6. do

Insert

calmodulin [Xenopus laevis] (calm2 Frog)

Expression

Mammalian

Mutation

D20A/D56A

Available Since

Sept. 21, 2018

Availability

Academic Institutions and Nonprofits only

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pEN-R2-mCsfGFP-L3

Plasmid

#178602

Purpose

It can be used to study protein turnover in living plant cells of Arabidopsis (Arabidopsis thaliana) and Nicotiana benthamiana.

Depositor

Frederica Theodoulou

Article

Zhang et al Plant Physiol. 2019 Jun;180(2):718-731.

Insert

Tandem fluorescent protein timers (tFTs)

Use

Entry vector for gateway cloning

Tags

mCherry superfolder GFP

Available Since

Dec. 17, 2021

Availability

Academic Institutions and Nonprofits only

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pFSynWSyt1C2AB(80 JuxtaK-421)msGFP

Plasmid

#209016

Purpose

Overexpression of truncated syt1 with Juxta K mutations and tagged with GFP in mammalian cells

Depositor

Edwin Chapman

Article

Mehta et al Nat Commun. 2024 Jan 4;15(1):262. doi: 1

Insert

syt1 (Syt1 Rat)

Use

Lentiviral

Tags

msfGFP

Expression

Mammalian

Mutation

aa 80-421 only, Juxta K mutant (80KKCLFKKKNKKKGKE…

Available Since

Nov. 9, 2023

Availability

Academic Institutions and Nonprofits only

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pQC.Flip-nluc(LgBiT1-8)CP[Casp3A(D5A)]-Fluc

Plasmid

#200124

Purpose

Retroviral expression of Flip-nluc(LgBiT1-8)CP[Casp3A(D5A)] protease reporter and P2A firefly luciferase in mammalian cells

Depositor

Eiji Morita

Article

Arakawa et al Sci Rep. 2023 Jan 31;13(1):1753. doi: 10

Insert

Flip-nluc(LgBiT1-8)CP[Casp3A(D5A)] P2A firefly luciferase

Use

Retroviral

Expression

Mammalian

Promoter

CMV

Available Since

May 11, 2023

Availability

Academic Institutions and Nonprofits only

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pQC.Flip-nluc(LgBiT1-8)CP[CoVA]-Fluc

Plasmid

#200125

Purpose

Retroviral expression of Flip-nluc(LgBiT1-8)CP[CoVA] protease reporter and P2A firefly luciferase in mammalian cells

Depositor

Eiji Morita

Article

Arakawa et al Sci Rep. 2023 Jan 31;13(1):1753. doi: 10

Insert

Flip-nluc(LgBiT1-8)CP[CoVA] P2A firefly luciferase

Use

Retroviral

Expression

Mammalian

Promoter

CMV

Available Since

May 11, 2023

Availability

Academic Institutions and Nonprofits only

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pEGFP-TdIF2

Plasmid

#199699

Purpose

visualization of nucleolus in living mammalian cells

Depositor

Kenji Sugimoto

Article

Fukada et al Biosci Biotechnol Biochem. 2019 Jul;83(7

Insert

TdIF2

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available Since

May 2, 2023

Availability

Academic Institutions and Nonprofits only

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pBEAST_J23101-bphD

Plasmid

#128138

Purpose

The cell-free adapted backbone, pBEAST, expressing bphD (the enzyme converting 2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoate to benzoate) under control of the constitutive promoter J23101, and RBS B0032

Depositor

Jean-Loup Faulon

Article

Pandi et al Nat Commun. 2019 Aug 28;10(1):3880. doi:

Insert

bphD

Use

Synthetic Biology

Expression

Bacterial

Promoter

J23101

Available Since

Aug. 28, 2019

Availability

Academic Institutions and Nonprofits only

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pHSG1C3

Plasmid

#164423

Purpose

single guide RNA (sgRNA) and Prime editing guide RNA (pegRNA) cloning and mammalian cell expression. BbsI cloning for sgRNAs and BbsI/PstI for pegRNAs.

Depositor

Xiao Wang

Article

Standage-Beier et al ACS Synth Biol. 2021 Feb 19;10(2):422-42

Insert

sgRNA

Use

CRISPR and Synthetic Biology

Expression

Mammalian

Promoter

U6

Available Since

Aug. 4, 2021

Availability

Academic Institutions and Nonprofits only

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