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pLAP-BUBR1-D882N

Plasmid

#114062

Purpose

transfection in mammalian cells, combination of fluorescent protein fusion and tandem affinity purification

Depositor

Geert Kops

Article

Suijkerbuijk et al Dev Cell. 2012 Jun 12;22(6):1321-9. doi:

Insert

BUBR1-D882N (BUB1B Human)

Tags

YFP-TEV-S- Human LAP

Expression

Mammalian

Mutation

C2823A and G2826A (siRNA-resistance), BUBR1-D882N

Available Since

Nov. 7, 2018

Availability

Academic Institutions and Nonprofits only

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pLAP-BUBR1-K795R

Plasmid

#114060

Purpose

transfection in mammalian cells, combination of fluorescent protein fusion and tandem affinity purification

Depositor

Geert Kops

Article

Suijkerbuijk et al Dev Cell. 2012 Jun 12;22(6):1321-9. doi:

Insert

BUBR1-K795R (BUB1B Human)

Tags

YFP-TEV-S- Human LAP

Expression

Mammalian

Mutation

C2823A and G2826A (siRNA-resistance), BUBR1-K795R

Available Since

Nov. 7, 2018

Availability

Academic Institutions and Nonprofits only

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pHAGE-RET-G533D

Plasmid

#116667

Purpose

Lentiviral expression of RET G533D

Depositor

Gordon Mills, Kenneth Scott

Article

Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

Insert

RET (RET Human)

Use

Lentiviral

Mutation

G533D

Available Since

March 14, 2019

Availability

Academic Institutions and Nonprofits only

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pHAGE-RET-G751E

Plasmid

#116668

Purpose

Lentiviral expression of RET G751E

Depositor

Gordon Mills, Kenneth Scott

Article

Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

Insert

RET (RET Human)

Use

Lentiviral

Mutation

G751E

Available Since

March 14, 2019

Availability

Academic Institutions and Nonprofits only

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pHAGE-RET-V121I

Plasmid

#116670

Purpose

Lentiviral expression of RET V121I

Depositor

Gordon Mills, Kenneth Scott

Article

Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

Insert

RET (RET Human)

Use

Lentiviral

Mutation

V121I

Available Since

March 14, 2019

Availability

Academic Institutions and Nonprofits only

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pHAGE-RET-V648I

Plasmid

#116671

Purpose

Lentiviral expression of RET V648I

Depositor

Gordon Mills, Kenneth Scott

Article

Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

Insert

RET (RET Human)

Use

Lentiviral

Mutation

V648I

Available Since

March 14, 2019

Availability

Academic Institutions and Nonprofits only

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pHAGE-RET-A641T

Plasmid

#116664

Purpose

Lentiviral expression of RET A641T

Depositor

Gordon Mills, Kenneth Scott

Article

Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

Insert

RET (RET Human)

Use

Lentiviral

Mutation

A641T

Available Since

March 14, 2019

Availability

Academic Institutions and Nonprofits only

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pHAGE-RET-E623K

Plasmid

#116665

Purpose

Lentiviral expression of RET E623K

Depositor

Gordon Mills, Kenneth Scott

Article

Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

Insert

RET (RET Human)

Use

Lentiviral

Mutation

E623K

Available Since

March 14, 2019

Availability

Academic Institutions and Nonprofits only

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pHAGE-RET-E901K

Plasmid

#116666

Purpose

Lentiviral expression of RET E901K

Depositor

Gordon Mills, Kenneth Scott

Article

Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

Insert

RET (RET Human)

Use

Lentiviral

Mutation

E901K

Available Since

March 14, 2019

Availability

Academic Institutions and Nonprofits only

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pEAHISPAFabH

Plasmid

#97018

Purpose

Expressing Pseudomonas aeruginosa FabH protein

Depositor

Huanting Liu, James Naismith

Article

Oke et al J Struct Funct Genomics. 2010 Jun;11(2):

Insert

3-Oxoacyl-[acyl-carrier-protein] synthase III

Tags

N-ter TEV protease cleavable 6HIs

Mutation

none

Promoter

T7

Available Since

Aug. 7, 2017

Availability

Academic Institutions and Nonprofits only

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pMSCV40-EF1a-hBCL10(1-185)-SO

Plasmid

#75258

Purpose

retroviral expression vector for expression of StrepOne-Tagged human BCL10 (AS 1-185)

Depositor

Ria Baumgrass

Article

Gabriel et al J Biol Chem. 2016 Sep 16. pii: jbc.M116.

Insert

Bcl10 (BCL10 Human)

Use

Retroviral

Tags

StrepOne Tag

Expression

Mammalian

Mutation

AS 1-185 (w/o C-terminus)

Promoter

pMSCV-LTRs, EF1a

Available Since

June 8, 2016

Availability

Academic Institutions and Nonprofits only

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pHAGE-PTCH1-T1404A

Plasmid

#116615

Purpose

Lentiviral expression of PTCH1 T1404A

Depositor

Gordon Mills, Kenneth Scott

Article

Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

Insert

PTCH1 (PTCH1 Human)

Use

Lentiviral

Mutation

T1404A

Available Since

March 14, 2019

Availability

Academic Institutions and Nonprofits only

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pHAGE-PTEN-R173C

Plasmid

#116632

Purpose

Lentiviral expression of PTEN R173C

Depositor

Gordon Mills, Kenneth Scott

Article

Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

Insert

PTEN (PTEN Human)

Use

Lentiviral

Mutation

R173C

Available Since

March 14, 2019

Availability

Academic Institutions and Nonprofits only

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pTH752-CEN-RLuc/min103maxCFLuc

Plasmid

#38218

Depositor

Tobias von der Haar

Article

Chu et al EMBO J. 2014 Jan 1;33(1):21-34. doi: 10.

Inserts

Firefly Luciferase
Renilla luciferase

Expression

Yeast

Mutation

The first 103 codons of the original FLuc gene we…

Promoter

ADH1 and TDH3 (=GPD)

Available Since

Oct. 22, 2012

Availability

Academic Institutions and Nonprofits only

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pTH751-CEN-RLuc/min53maxCFLuc

Plasmid

#38217

Depositor

Tobias von der Haar

Article

Chu et al EMBO J. 2014 Jan 1;33(1):21-34. doi: 10.

Inserts

Firefly Luciferase
Renilla luciferase

Expression

Yeast

Mutation

The first 53 codons of the original FLuc gene wer…

Promoter

ADH1 and TDH3 (=GPD)

Available Since

Oct. 22, 2012

Availability

Academic Institutions and Nonprofits only

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NFATc1-CRISPR-Nick 1/2 (PX461-EF1a-pSpCas9n(BB)-2A-GFP)

Plasmid

#75238

Purpose

CRISPR/Cas9 NICKASE plasmid against human NFATc1 (1/2)

Depositor

Ria Baumgrass

Article

Gabriel et al J Biol Chem. 2016 Sep 16. pii: jbc.M116.

Insert

sgRNA against NFATc1 (NFATC1 Human)

Use

CRISPR

Available Since

June 8, 2016

Availability

Academic Institutions and Nonprofits only

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NFATc1-CRISPR-Nick 2/2 (PX461-EF1a-pSpCas9n(BB)-2A-GFP)

Plasmid

#75239

Purpose

CRISPR/Cas9 NICKASE plasmid against human NFATc1 (2/2)

Depositor

Ria Baumgrass

Article

Gabriel et al J Biol Chem. 2016 Sep 16. pii: jbc.M116.

Insert

sgRNA against NFATc1 (NFATC1 Human)

Use

CRISPR

Available Since

June 8, 2016

Availability

Academic Institutions and Nonprofits only

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pCBS-5753

Plasmid

#249325

Purpose

To express the EPEC dDarT2(DLAA)-nScCas9(D10A) fusion protein in experiments for Append Editing

Depositor

Chase Beisel

Article

Gupta et al Nat Biotechnol. 2025 Sep 4. doi: 10.1038

Insert

EPEC dDarT2(DLAA)-nScCas9(D10A)

Expression

Yeast

Mutation

DarT2(G49D, M86L, R92A, E,170A, R193A) and nScCas…

Available Since

Jan. 7, 2026

Availability

Academic Institutions and Nonprofits only

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pLenti-gRNA2-4

Plasmid

#248525

Purpose

A CRISPR-based technology to study chromosome-specific aneuploidy by targeting human centromeres

Depositor

Teresa Davoli

Article

Bosco et al Cell. 2023 Apr 27;186(9):1985-2001.e19.

Insert

gRNA2-4

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

Jan. 5, 2026

Availability

Academic Institutions and Nonprofits only

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pLenti-gRNAY-4

Plasmid

#248556

Purpose

A CRISPR-based technology to study chromosome-specific aneuploidy by targeting human centromeres

Depositor

Teresa Davoli

Article

Bosco et al Cell. 2023 Apr 27;186(9):1985-2001.e19.

Insert

gRNAY-4

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

Dec. 18, 2025

Availability

Academic Institutions and Nonprofits only

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