We narrowed to 12,024 results for: pcdna

1. pCDNA4TO-3XFLAG Halo R99A nsp1

   Plasmid

   #175422

   Purpose

   Expresses R99A CoV2 nsp1 in mammalian cells. This contains a 3XFLAG Halo tag fusion to nsp1.

   Depositor

   Britt Glaunsinger

   Article

   Mendez et al Cell Rep. 2021 Oct 19;37(3):109841. doi:

   Insert

   CoV2 R99A nsp1
   

   Use

   Tags

   3XFLAG Halo

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Oct. 8, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pcDNA3.1 LIC 6A CARD8-FLAG F405G

   Plasmid

   #169956

   Purpose

   Mammalian expression of CARD8-FLAG with a site III mutation (F405G)

   Depositor

   Hao Wu

   Article

   Sharif et al Immunity. 2021 Jul 13;54(7):1392-1404.e1

   Insert

   CARD8 (CARD8 Human)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   F405G

   Promoter

   CMV

   Available Since

   Sept. 14, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pcDNA3.1-2xFLAG-2xSTREP-CCND1(P287S)

   Plasmid

   #172644

   Purpose

   Expresses 2xFLAG-2xSTREP-tagged cyclin D1(P287S) in mammalian cells

   Depositor

   Michele Pagano

   Article

   Simoneschi et al Nature. 2021 Apr;592(7856):789-793. doi:

   Insert

   CCND1 (CCND1 Human)
   

   Use

   Tags

   2xFLAG-2xSTREP

   Expression

   Mammalian

   Mutation

   Pro287Ser

   Promoter

   CMV

   Available Since

   Aug. 20, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pcDNA3.1-2xFLAG-2xSTREP-CCND1(T286I)

   Plasmid

   #172645

   Purpose

   Expresses 2xFLAG-2xSTREP-tagged cyclin D1(T286I) in mammalian cells

   Depositor

   Michele Pagano

   Article

   Simoneschi et al Nature. 2021 Apr;592(7856):789-793. doi:

   Insert

   CCND1 (CCND1 Human)
   

   Use

   Tags

   2xFLAG-2xSTREP

   Expression

   Mammalian

   Mutation

   Thr286Ile

   Promoter

   CMV

   Available Since

   Aug. 20, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pcDNA3.1-2xFLAG-2xSTREP-CCND1(P287L)

   Plasmid

   #172646

   Purpose

   Expresses 2xFLAG-2xSTREP-tagged cyclin D1(P287L) in mammalian cells

   Depositor

   Michele Pagano

   Article

   Simoneschi et al Nature. 2021 Apr;592(7856):789-793. doi:

   Insert

   CCND1 (CCND1 Human)
   

   Use

   Tags

   2xFLAG-2xSTREP

   Expression

   Mammalian

   Mutation

   Pro287Leu

   Promoter

   CMV

   Available Since

   Aug. 20, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pcDNA3HA-AMOT p130∆PPxY1(L106P)

   Plasmid

   #166714

   Purpose

   expresses AMOT(L106P) in mammalian cells

   Depositor

   Wesley Sundquist

   Article

   Rheinemann et al J Biol Chem. 2021 Jul 17:100975. doi: 10

   Insert

   Angiomotin p130 L106P (AMOT Human)
   

   Use

   Tags

   2xHA

   Expression

   Mammalian

   Mutation

   changed leucine 106 to proline

   Promoter

   CMV promoter

   Available Since

   July 23, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pcDNA3HA-AMOT p130∆PPxY1(A112K)

   Plasmid

   #166715

   Purpose

   expresses AMOT(A112K) in mammalian cells

   Depositor

   Wesley Sundquist

   Article

   Rheinemann et al J Biol Chem. 2021 Jul 17:100975. doi: 10

   Insert

   Angiomotin p130 A112K (AMOT Human)
   

   Use

   Tags

   2xHA

   Expression

   Mammalian

   Mutation

   changed alanine 112 to lysine

   Promoter

   CMV promoter

   Available Since

   July 23, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCDNA3_shRNA resistant PKA-Cα(E12,14K)-mEGFP

   Plasmid

   #168491

   Purpose

   Mammalian expression of the E12,14K mutant of the mouse PKA catalytic subunit α C-terminally tagged by monomeric EGFP. Silent mutations were introduced to make it resistant to shRNA knock-down.

   Depositor

   Haining Zhong

   Article

   Xiong et al Proc Natl Acad Sci U S A. 2021 Apr 13;11

   Insert

   the E12,14K mutant of the mouse PKA catalytic subunit α C-terminally tagged by mEGFP. Resistant to shRNA knock-down.
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   May 11, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pcDNA3.1 LIC 6A CARD8-FLAG R394E

   Plasmid

   #169954

   Purpose

   Mammalian expression of CARD8-FLAG with a site III mutation (R394E)

   Depositor

   Hao Wu

   Article

   Sharif et al Immunity. 2021 Jul 13;54(7):1392-1404.e1

   Insert

   CARD8 (CARD8 Human)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   R394E

   Promoter

   CMV

   Available Since

   May 11, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pcDNA3.1 LIC 6A CARD8 Ub-S297-FLAG

    Plasmid

    #169958

    Purpose

    Mammalian expression of the CARD8 C-terminus fused to ubiquitin (co-translationally processed)

    Depositor

    Hao Wu

    Article

    Sharif et al Immunity. 2021 Jul 13;54(7):1392-1404.e1

    Insert

    CARD8 (CARD8 Human)
    

    Use

    Tags

    FLAG and Ub

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    May 11, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pcDNA3.1 LIC 6A CARD8-FLAG L368G

    Plasmid

    #169952

    Purpose

    Mammalian expression of CARD8-FLAG with a site III mutation (L368G)

    Depositor

    Hao Wu

    Article

    Sharif et al Immunity. 2021 Jul 13;54(7):1392-1404.e1

    Insert

    CARD8 (CARD8 Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    L368G

    Promoter

    CMV

    Available Since

    May 11, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pcDNA3.1 LIC 6A CARD8-FLAG E274R

    Plasmid

    #169950

    Purpose

    Mammalian expression of CARD8-FLAG that cannot bind DPP9 due to a mutation at site I (E274R)

    Depositor

    Hao Wu

    Article

    Sharif et al Immunity. 2021 Jul 13;54(7):1392-1404.e1

    Insert

    CARD8 (CARD8 Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    E274R

    Promoter

    CMV

    Available Since

    May 11, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pcDNA3.1 LIC 6A CARD8-FLAG F370G

    Plasmid

    #169953

    Purpose

    Mammalian expression of CARD8-FLAG with a site III mutation (F370G)

    Depositor

    Hao Wu

    Article

    Sharif et al Immunity. 2021 Jul 13;54(7):1392-1404.e1

    Insert

    CARD8 (CARD8 Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    F370G

    Promoter

    CMV

    Available Since

    May 11, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pcDNA3-3NFLAG-mLIG3-179-936

    Plasmid

    #159316

    Purpose

    Mammalian cell expression of mouse LIG3-179-936

    Depositor

    Jianjun Zhao

    Insert

    mLIG3-179-936 (Lig3 Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    April 12, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pcDNA4/TO-2xStrep-ORF68 K450A/R451A

    Plasmid

    #162638

    Purpose

    Expresses N-terminal 2x-Strep-tagged ORF68 K450A/R451A in mammalian cells

    Depositor

    Britt Glaunsinger

    Article

    Didychuk et al Elife. 2021 Feb 8;10. pii: 62261. doi: 1

    Insert

    ORF68
    

    Use

    Tags

    N-terminal 2xStrep-Tag II

    Expression

    Mammalian

    Mutation

    Amino acids K450 and R451 mutated to alanine

    Promoter

    CMV

    Available Since

    Feb. 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pcDNA4/TO-2xStrep-ORF68 R14A/K310A

    Plasmid

    #162642

    Purpose

    Expresses N-terminal 2x-Strep-tagged ORF68 R14A/K310A in mammalian cells

    Depositor

    Britt Glaunsinger

    Article

    Didychuk et al Elife. 2021 Feb 8;10. pii: 62261. doi: 1

    Insert

    ORF68
    

    Use

    Tags

    N-terminal 2xStrep-Tag II

    Expression

    Mammalian

    Mutation

    Amino acids R14 and K310 mutated to alanine

    Promoter

    CMV

    Available Since

    Feb. 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pcDNA4/TO-2xStrep-ORF68 K395A/K396A

    Plasmid

    #162643

    Purpose

    Expresses N-terminal 2x-Strep-tagged ORF68 K395A/K396A in mammalian cells

    Depositor

    Britt Glaunsinger

    Article

    Didychuk et al Elife. 2021 Feb 8;10. pii: 62261. doi: 1

    Insert

    ORF68
    

    Use

    Tags

    N-terminal 2xStrep-Tag II

    Expression

    Mammalian

    Mutation

    Amino acids K395 and K396 mutated to alanine

    Promoter

    CMV

    Available Since

    Feb. 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pcDNA4/TO-2xStrep-UL32 C308A/C309A

    Plasmid

    #162646

    Purpose

    Expresses N-terminal 2x-Strep-tagged UL32 C308A/C309A in mammalian cells

    Depositor

    Britt Glaunsinger

    Article

    Didychuk et al Elife. 2021 Feb 8;10. pii: 62261. doi: 1

    Insert

    UL32
    

    Use

    Tags

    N-terminal 2xStrep-Tag II

    Expression

    Mammalian

    Mutation

    Amino acids C308 and C309 mutated to alanine

    Promoter

    CMV

    Available Since

    Feb. 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pcDNA4/TO-2xStrep-ORF68 C191A/C192A

    Plasmid

    #162632

    Purpose

    Expresses N-terminal 2x-Strep-tagged ORF68 C191A/C192A in mammalian cells

    Depositor

    Britt Glaunsinger

    Article

    Didychuk et al Elife. 2021 Feb 8;10. pii: 62261. doi: 1

    Insert

    ORF68
    

    Use

    Tags

    N-terminal 2xStrep-Tag II

    Expression

    Mammalian

    Mutation

    Amino acids C191 and C192 mutated to alanine

    Promoter

    CMV

    Available Since

    Feb. 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pcDNA3.1-N-Myc_DW164AA + DED/AAA USP7

    Plasmid

    #131256

    Purpose

    Mammalian expression of N-terminally Myc-tagged USP7, with mutations in the TRAF-like domain (DW164AA) and UBL2 (DE758AA_D764A)

    Depositor

    Roger Woodgate

    Article

    Ashton et al J Mol Biol. 2021 Jan 22;433(2):166733. d

    Insert

    Ubiquitin carboxyl-terminal hydrolase 7 (USP7 Human)
    

    Use

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    Contains point mutations that convert USP7 aspart…

    Promoter

    Available Since

    Dec. 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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