We narrowed to 14,370 results for: bli
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Plasmid#216801PurposeExpresses a single point mutant of melanoma differentiation-associated protein 5 lacking its 2 CARD domainsDepositorInsertmelanoma differentiation-associated protein 5 delta 2 CARD domains (IFIH1 Human)
ExpressionMammalianMutationG495R/∆1-287Available SinceMay 17, 2024AvailabilityAcademic Institutions and Nonprofits only -
CAPZB2-TwinStrepII
Plasmid#83194PurposeExpression of tagged protein in mammalian cellsDepositorAvailable SinceSept. 23, 2016AvailabilityAcademic Institutions and Nonprofits only -
LIMK1
Plasmid#39181PurposeBaculovirus expression for structure determination; may not be full ORFDepositorAvailable SinceJune 7, 2013AvailabilityAcademic Institutions and Nonprofits only -
pcDNA_DiCre2_59.F2
Plasmid#60209PurposeExpresses a fusion protein of FKBP12(F36V)-linker peptide 2- Cre(19-59)DepositorInsertCre recombinase (CD207 Human, Bacteriophage P1, Synthetic)
TagsFKBP12(F36V) and Nuclear localisation signalExpressionMammalianMutationFKBP12 has the F36V mutation; amino acids 19-59 o…PromoterCMVAvailable SinceJan. 8, 2015AvailabilityAcademic Institutions and Nonprofits only -
pLenti.puro.MCV.ER.RAZ.1
Plasmid#114381PurposeThis lentiviral plasmid expresses MCV tumor T antigens (truncated LT and small T) using the viral NCCR (non-coding control region). The sequence was amplifeied from MCV.R17a and truncated LT=330 aa.DepositorInsertMCV NCCR and tumor T antigen regions
UseLentiviralExpressionMammalianPromoterMCV NCCR (viral own promoter)Available SinceSept. 13, 2018AvailabilityAcademic Institutions and Nonprofits only -
ECH1
Plasmid#38911PurposeBacterial expression for structure determination; may not be full ORFDepositorAvailable SinceMarch 18, 2013AvailabilityAcademic Institutions and Nonprofits only -
raft-APEX2
Plasmid#205089PurposeExpresses lipid-raft targeted APEX2 for proximity proteomics. Vector: pcDNA™4/TO. Insert: lipid raft target sequence (MGCVCSS) + mCherry +V5 (GKPIPNPLLGLDST) +APEX2DepositorInsertAPEX2 (raft-mCherry-V5 tagged)
Tagslipid raft targeting sequence (MGCVCSS), mCherry,…ExpressionMammalianAvailable SinceApril 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCSD_N-SpCas9-1-573-FRB-1xNLS
Plasmid#107298PurposeExpresses N terminal SpCas9 (1-573 aa) fused to FRB in mammalian cellsDepositorInsertTruncated SpCas9 (aa 1-573) fused to FRB
UseCRISPRTagsFRB and SV40 NLSExpressionMammalianMutationTruncated SpCas9 (aa 1-573)PromoterCMV IE94Available SinceNov. 15, 2018AvailabilityAcademic Institutions and Nonprofits only -
K560_AviN_coBirA
Plasmid#129766PurposeExpresses Drosophila Kinesin-1 truncated at 560 and biotinylated on the head for single-molecule assaysDepositorInsertKHC (Khc Fly)
TagsAvi-tag GLNDIFEAQKIEWH and His6ExpressionBacterialMutationTruncation at 559Available SinceNov. 20, 2019AvailabilityAcademic Institutions and Nonprofits only -
miniSOG-Farnesyl-5
Plasmid#62782PurposeLocalization: Membrane, Excitation:448 / 473, Emission:500 / 528DepositorAvailable SinceMarch 10, 2015AvailabilityIndustry, Academic Institutions, and Nonprofits -
APPL1-N308D-GFP
Plasmid#102783PurposePoint mutation in APPL1 that diminishes its interaction with Rab5DepositorInsertAPPL1 (APPL1 Human)
TagsEGFPExpressionMammalianMutationchanged asparagine 308 to aspartate; *(see below)PromoterCMVAvailable SinceJan. 16, 2018AvailabilityAcademic Institutions and Nonprofits only -
ASH1L
Plasmid#39051PurposeBacterial expression for structure determination; may not be full ORFDepositorAvailable SinceMarch 18, 2013AvailabilityAcademic Institutions and Nonprofits only -
AAV_CWSL.nEF.DIO.Synaptophysin-GCaMP6s.P2A.mRuby3
Plasmid#107736PurposeIn the presence of Cre, can be used to express both GCaMP6s fused to the presynaptic protein synaptophysin and independently the red-fluorescent protein mRuby3.DepositorInsertsynaptophysin-GCaMP6s, mRuby3
UseAAV and Cre/LoxExpressionMammalianPromoternEFAvailable SinceMarch 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
pMAL-c5X-BRT17
Plasmid#67000PurposeExpresses malE-BRT17 in E. coliDepositorInsertmalE-BRT17
TagsHis6 and malE-Factor XaExpressionBacterialPromotertacAvailable SinceJan. 5, 2016AvailabilityAcademic Institutions and Nonprofits only -
4L59
Plasmid#60087PurposeBacterial expression for structure determination; may not be full ORFDepositorInsertL3MBTL3:JMC057-A08:C223215 (L3MBTL3 Human)
Tags6xHisExpressionBacterialMutationSee commentsPromoterT7Available SinceOct. 22, 2014AvailabilityIndustry, Academic Institutions, and Nonprofits -
pB6-Hipp11-td-sfGFP dre reporter-FNF-DTA
Plasmid#139513PurposeB6J Hipp11-pCAG-rox-FNF-bGHpA-3xSV40pA-rox-td-sfGFP-WPRE-bpA allele targeting vector, low copy numberDepositorInsertCAG rox stop rox td-sfGFP dre reporter
UseMouse Targeting; Dre/roxExpressionMammalianAvailable SinceNov. 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
pJK372
Plasmid#73597PurposeEncodes Crepis alpina delta-12 fatty acid acetylenase (vFAD2) with C-terminal FLAG epitope and delta-15 fatty acid desaturase (FAD3) with C-terminal c-myc epitopeDepositorInsertsdelta-12 fatty acid acetylenase
delta-15 fatty acid desaturase
TagsFLAG tag and c-myc tagExpressionYeastPromoterGAL1 and GAL10Available SinceFeb. 26, 2016AvailabilityAcademic Institutions and Nonprofits only -
DAPK3
Plasmid#38964PurposeBacterial expression for structure determination; may not be full ORFDepositorAvailable SinceMarch 18, 2013AvailabilityAcademic Institutions and Nonprofits only -
isoform PKC Delta (JCE594)
Plasmid#182025Purposeyeast expression of mouse PKC Delta with yeast PKC1 promoter and ADH1 terminatorDepositorAvailable SinceMay 17, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET28a-HIS-TagGFP2-TEV-GGG-ELP(40nm)-Cys
Plasmid#90474PurposeElastin Like Polypeptide: [(VPGEG)-(VPGVG)4-(VPGAG)2-(VPGGG)2-(VPGEG)]2, with N-Terminal TagGFP2 and TEV-cleavage site, leaving an N-terminal Sortase Recognition Sequence GGG and a C-terminal CysteineDepositorInsertTagGFP2-TEV-GGG-ELP(40nm)-Cys
TagsCysteine and Sortase-TagExpressionBacterialPromoterT7Available SinceJuly 7, 2017AvailabilityAcademic Institutions and Nonprofits only