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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 152,794 results for: ins

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Showing: 6361 - 6380 of 152794 results

  2. HSC1-HS4-GiP

    Plasmid
    #58540
    Purpose
    Derived from HSC1-GiP where 2 copies of the HS4 insulator core are cloned inside the 3'LTR
    Depositor
    James Ellis
    Article
    Rival-Gervier et al Mol Ther. 2013 Aug;21(8):1536-50. doi: 1
    Inserts
    Enhanced Green Fluorescence Protein, Puromycin resistance gene
    Core HS4 insulator sequence (dimer)
    Use
    Retroviral
    Expression
    Mammalian
    Promoter
    Human EF1-alpha
    Available Since
    Sept. 3, 2014
    Availability
    Academic Institutions and Nonprofits only
    58540-plasmid-map-sequence-id-90629

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  4. MSP712

    Plasmid
    #65768
    Purpose
    Bacterial expression plasmid for Sp-dCas9 & sgRNA (need to clone in spacer into BsaI sites): T7-humanSpdCas9(D10A/H840A)-T7-BsaIcassette-Sp-sgRNA
    Depositor
    Keith Joung
    Article
    Kleinstiver et al Nature. 2015 Jun 22. doi: 10.1038/nature
    Insert
    mammalian codon-optimized Streptococcus pyogenes dCas9 (D10A/H840A)-NLS-3XFlag, and SpCas9 gRNA
    Use
    CRISPR
    Tags
    3x FLAG and NLS
    Expression
    Bacterial
    Mutation
    D10A and H840A mutations in Cas9
    Promoter
    T7 (x2)
    Available Since
    June 24, 2015
    Availability
    Academic Institutions and Nonprofits only
    65768-plasmid-map-sequence-id-112655

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  5. pACEBac1-CoRESTfl

    Plasmid
    #109156
    Purpose
    Encodes human full-length CoREST with C-terminal 3xFLAG tag to be expressed in a baculovirus/insect cell expression system
    Depositor
    Monika Golas
    Article
    Inui et al Protein Sci. 2017 May;26(5):997-1011. do
    Insert
    human full-length CoREST, sequence-optimized for insect cells (RCOR1 Human)
    Tags
    3xFLAG
    Expression
    Insect
    Promoter
    Polyhedrin
    Available Since
    Jan. 11, 2019
    Availability
    Academic Institutions and Nonprofits only
    109156-plasmid-map-sequence-id-232884

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  6. MSP2292

    Plasmid
    #70707
    Purpose
    Bacterial expression plasmid for KKH SaCas9 & sgRNA targeted to site 2: T7-humanSaCas9(E782K/N968K/R1015H)-NLS-3xFLAG-T7-Sa-sgRNA(84) #2
    Depositor
    Keith Joung
    Article
    Kleinstiver et al Nat Biotechnol. 2015 Nov 2. doi: 10.1038
    Inserts
    mammalian codon-optimized KKH variant Staphylococcus aureus Cas9
    SaCas9 sgRNA (+84)
    Use
    CRISPR
    Tags
    NLS-3xFLAG
    Expression
    Bacterial
    Mutation
    E782K, N968K and R1015H in SaCas9
    Promoter
    T7
    Available Since
    Dec. 23, 2015
    Availability
    Academic Institutions and Nonprofits only
    70707-plasmid-map-sequence-id-127770

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  15. pcpAzpaRS_v2

    Plasmid
    #196483
    Purpose
    To express azidophenylalanine-specific variant of E. coli TyrRS (pAzpaRS) and its cognate amber suppressor tRNA to allow UAG codon to be translated into azidophenylalanine.
    Depositor
    Kensaku Sakamoto
    Article
    Hino et al Methods Mol Biol. 2012;794:215-28. doi:
    Insert
    azidophenylalanine-specific variant of E. coli TyrRS (pAzpaRS), amber suppressor tRNA
    Expression
    Mammalian
    Promoter
    SV40(synthetase), Pol III tRNA promoter (tRNA), C…
    Available Since
    Jan. 12, 2024
    Availability
    Academic Institutions and Nonprofits only
    196483-plasmid-map-sequence-id-388871

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  18. RH5-bio

    Plasmid
    #47780
    Purpose
    Expresses enzymatically monobiotinylated full-length RH5 with no N-linked glycans upon cotransfection with BirA in mammalian cells. Contains a C-terminal rat Cd4 d3+4 tag.
    Depositor
    Gavin Wright
    Article
    Crosnier et al Mol Cell Proteomics. 2013 Sep 16.
    Insert
    Codon-optimised RH5
    Tags
    enzymatic biotinylation sequence and rat Cd4 d3+4
    Expression
    Mammalian
    Mutation
    Exogenous signal peptide of mouse origin, changed…
    Promoter
    CMV
    Available Since
    Feb. 4, 2014
    Availability
    Academic Institutions and Nonprofits only
    47780-plasmid-map-sequence-id-68799

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  19. 908H

    Plasmid
    #160240
    Purpose
    TetO(2x)-Hsp70min-UASpmin-Firefly-T2A-eGFP-p10-3’UTR-Ubiquitin-Renilla-attB
    Depositor
    Omar Akbari
    Article
    Gamez et al Insect Mol Biol. 2021 Oct;30(5):461-471.
    Inserts
    Tet Operator (2x)
    hsp70 minimal promoter
    p-element promoter
    Firefly luciferase
    Renilla luciferase
    Ubiquitin promoter
    Tags
    T2A GFP
    Expression
    Insect
    Available Since
    Nov. 23, 2020
    Availability
    Academic Institutions and Nonprofits only
    160240-plasmid-map-sequence-id-316017

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  20. Nrxn3α-AS4-

    Plasmid
    #184332
    Purpose
    Expression of mouse Nrx3-alpha lacking insertion at the alternative splice site 4 together with EGFP protein separated by 2A peptide. Contains insertions at alternatively splice sites 1,2,3 and 6.
    Depositor
    Peter Scheiffele
    Article
    Nguyen et al Elife. 2016 Dec 13;5. doi: 10.7554/eLife
    Insert
    Nrxn3-alpha (Nrxn3 Mouse)
    Tags
    HA-tag
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    June 6, 2022
    Availability
    Academic Institutions and Nonprofits only
    184332-plasmid-map-sequence-id-361409

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Showing: 6361 - 6380 of 152794 results