We narrowed to 15,028 results for: 112

1. pRK5 Halo RagB

   Plasmid

   #112753

   Purpose

   express Halo-RagB

   Depositor

   Roberto Zoncu

   Article

   Lawrence et al Nat Cell Biol. 2018 Sep;20(9):1052-1063.

   Insert

   Halo-RagB (RRAGB Human)
   

   Tags

   Halo

   Expression

   Mammalian

   Available Since

   Oct. 15, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pGEX-2T-M

   Plasmid

   #1128

   Depositor

   Susan Lindquist

   Article

   Scheibel et al Nat Struct Biol 2001 Nov;8(11):958-62.

   Insert

   M (SUP35 Budding Yeast)
   

   Tags

   GST

   Expression

   Bacterial

   Available Since

   Jan. 6, 2005

   Availability

   Academic Institutions and Nonprofits only

3. pOP3TT

   Plasmid

   #112605

   Purpose

   Used for expression of protein in E. coli as TEV cleavable N-terminal Avi-His8-tag and thioredoxin fusions

   Depositor

   Marko Hyvönen

   Type

   Empty backbone

   Tags

   Avi - 8His-thioredoxin

   Expression

   Bacterial

   Available Since

   Aug. 27, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. pAA.3.lacIh_I12X86

   Plasmid

   #112025

   Purpose

   Expresses LacI_I12X86 protein

   Depositor

   Stephen Harrison

   Article

   Jenni et al Science. 2018 May 4;360(6388):552-558. d

   Insert

   LacI_I12X86
   

   Tags

   his

   Promoter

   T7

   Available Since

   July 17, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. gRNA[Sxl].1026B

   Plasmid

   #112688

   Purpose

   express gRNA targeting Sxl under dU6-3 promoter

   Depositor

   Omar Akbari

   Article

   Kandul et al Nat Commun. 2019 Jan 8;10(1):84. doi: 10

   Insert

   U6.3-gRNA[Sxl] (Sxl Fly)
   

   Use

   CRISPR

   Available Since

   Jan. 8, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. pcDNA3.1-hRIPK1-Flag-K115R

   Plasmid

   #112488

   Purpose

   Mammalian expression of the indicated Flag-hRIPK1 mutant. Please note Flag tag is N-terminal.

   Depositor

   Junying Yuan

   Insert

   RIPK1 (RIPK1 Human)
   

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   K115R

   Available Since

   July 6, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. pMM549

   Plasmid

   #112531

   Purpose

   pZA1D-hrtR-chuA - HrtR expressed consitutively from promoter ProD with RBS2, ChuA expressed consitiutively from promoter J23107, p15a origin, AmpR

   Depositor

   Timothy Lu

   Article

   Mimee et al Science. 2018 May 25;360(6391):915-918.

   Inserts

   HrtR
   ChuA
   

   Expression

   Bacterial

   Promoter

   J23107 and ProD

   Available Since

   Feb. 15, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pOP3BP

   Plasmid

   #112602

   Purpose

   Used for expression of protein in E. coli as PreScission cleavable N-terminal Avi-His8-tag and GB1 fusions

   Depositor

   Marko Hyvönen

   Type

   Empty backbone

   Tags

   Avi - 8His-GB1

   Expression

   Bacterial

   Available Since

   Aug. 27, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. pMAT9

   Plasmid

   #112590

   Purpose

   Used for expression of protein in E. coli as Thrombin cleavable N-termina 6His-tag-MBP fusions and without NcoI site within MBP.

   Depositor

   Johan Peränen, Marko Hyvönen

   Article

   Peranen et al Anal Biochem. 1996 May 1;236(2):371-3.

   Type

   Empty backbone

   Tags

   6His-MBP

   Expression

   Bacterial

   Available Since

   Aug. 27, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCLOCK.1.0-gDNA

    Plasmid

    #112406

    Purpose

    CRISPR plasmid for expression of Cas9 and gRNA targeting human transcription factor CLOCK

    Depositor

    Kevin White

    Insert

    CLOCK (CLOCK Human)
    

    Use

    CRISPR

    Expression

    Mammalian

    Available Since

    Dec. 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCLOCK-donor

    Plasmid

    #112310

    Purpose

    CRISPR donor plasmid to tag human transcription factor CLOCK with GFP

    Depositor

    Kevin White

    Insert

    CLOCK homology arms flanking EGFP-IRES-Neo cassette (CLOCK Human)
    

    Use

    CRISPR

    Mutation

    hg38:4:55435551:A>G, hg38:4:55435749:G>A

    Available Since

    Nov. 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. pFOXA3.1.0-gDNA

    Plasmid

    #112417

    Purpose

    CRISPR plasmid for expression of Cas9 and gRNA targeting human transcription factor FOXA3

    Depositor

    Kevin White

    Insert

    FOXA3 (FOXA3 Human)
    

    Use

    CRISPR

    Expression

    Mammalian

    Available Since

    Dec. 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. pFastBac His HA GNU WT

    Plasmid

    #112994

    Purpose

    Baculovirus expression of His/HA-tagged Drosophila gnu

    Depositor

    Terry Orr-Weaver

    Article

    Hara et al Elife. 2017 May 30;6. doi: 10.7554/eLife

    Insert

    gnu (gnu Fly)
    

    Tags

    His/HA

    Expression

    Insect

    Available Since

    Aug. 1, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. gRNA[bTub].1026C

    Plasmid

    #112691

    Purpose

    express gRNA targeting bTub under dU6-3 promoter

    Depositor

    Omar Akbari

    Article

    Kandul et al Nat Commun. 2019 Jan 8;10(1):84. doi: 10

    Insert

    U6.3-gRNA[bTub]
    

    Use

    CRISPR

    Promoter

    dU6-3

    Available Since

    Jan. 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. pLL3.7-EF-EYFP-YAP1_Y357F-PolyA

    Plasmid

    #112287

    Purpose

    Fluorescently labels human YAP1-2gamma isoform (504 aa) Y357F mutant _ corresponding to tyrosine 407 in this isoform

    Depositor

    Erik Sahai

    Article

    Ege et al Cell Syst. 2018 Jun 27;6(6):692-708.e13.

    Insert

    EYFP-YAP1 (YAP1 Human)
    

    Use

    Lentiviral

    Mutation

    Tyrosine 357 to Phenyalanine corresponding to tyr…

    Promoter

    EF-1 alpha

    Available Since

    May 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. pTGC-U6.2

    Plasmid

    #112809

    Purpose

    PCR template vector for amplifying gRNAcore-pU6.3 promoter fragment

    Depositor

    Chun Han

    Article

    Poe et al Genetics. 2018 Nov 30. pii: genetics.118

    Insert

    U6:2-gRNAcore
    

    Expression

    Bacterial

    Available Since

    Dec. 4, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCON1-ProC.mCherry

    Plasmid

    #112521

    Purpose

    ProC driven mCherry expression with synTT

    Depositor

    Olivia Steele-Mortimer

    Article

    Cooper et al Front Cell Infect Microbiol. 2017 Nov 16

    Insert

    ProC mCherry TT
    

    Expression

    Bacterial

    Promoter

    ProC

    Available Since

    Sept. 10, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. pTFCP2-donor

    Plasmid

    #112308

    Purpose

    CRISPR donor plasmid to tag human transcription factor TFCP2 with GFP

    Depositor

    Kevin White

    Insert

    TFCP2 homology arms flanking EGFP-IRES-Neo cassette (TFCP2 Human)
    

    Use

    CRISPR

    Available Since

    Dec. 17, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. pTFCP2.1.0-gDNA

    Plasmid

    #112404

    Purpose

    CRISPR plasmid for expression of Cas9 and gRNA targeting human transcription factor TFCP2

    Depositor

    Kevin White

    Insert

    TFCP2 (TFCP2 Human)
    

    Use

    CRISPR

    Expression

    Mammalian

    Available Since

    Dec. 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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20. pJW1671

    Plasmid

    #112045

    Purpose

    CEN/ARS plasmid for GAL-inducible ORM1-V5-TEV-EGFP expression marked with URA

    Depositor

    Jonathan Weissman

    Article

    Costa et al Science. 2018 Jan 18. pii: science.aar36

    Insert

    ORM1
    

    Tags

    V5-TEV-EGFP

    Expression

    Yeast

    Promoter

    GAL1

    Available Since

    Aug. 22, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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