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Human Mevalonate Kinase (MK)

Plasmid

#52429

Purpose

bacterial expression of human Mevalonate Kinase (MK)

Depositor

Henry Miziorko

Article

Potter et al J Biol Chem. 1997 Oct 10;272(41):25449-5

Insert

Human Mevalonate Kinase (MVK Human)

Expression

Bacterial

Mutation

see comments

Promoter

T7

Available Since

April 10, 2014

Availability

Academic Institutions and Nonprofits only
pSG lac10

Plasmid

#63875

Purpose

Measurements of translational accuracy

Depositor

Albert Dahlberg

Article

O'Connor et al Proc Natl Acad Sci U S A. 1993 Oct 1;90(

Insert

lacZ (lacZ Escherichia coli)

Expression

Bacterial

Mutation

-1 frameshift near 5' end

Promoter

PtacI

Available Since

April 27, 2015

Availability

Academic Institutions and Nonprofits only
pDL13

Plasmid

#17836

Depositor

Jay Keasling

Article

Lubertozzi et al Appl Microbiol Biotechnol. 2006 Oct . 72

Insert

alcA promoter, lacZ, trpC

Use

Synthetic Biology; A. nidulans

Available Since

May 14, 2008

Availability

Academic Institutions and Nonprofits only
Ube2d3

Plasmid

#31411

Depositor

Rachel Klevit

Article

Christensen et al Nat Struct Mol Biol. 2007 Oct . 14(10):9

Insert

Ubiquitin Conjugating Enzyme (UBE2D3 Human)

Tags

HA

Expression

Yeast

Available Since

Oct. 27, 2011

Availability

Academic Institutions and Nonprofits only
pDL12c

Plasmid

#17835

Depositor

Jay Keasling

Article

Lubertozzi et al Appl Microbiol Biotechnol. 2006 Oct . 72

Insert

gpdA promoter, lacZ, argB_1626,

Use

Synthetic Biology; Aspergillus nidulans expression

Mutation

The argB_1626 mutation consists of two sequential…

Available Since

May 14, 2008

Availability

Academic Institutions and Nonprofits only
p64-101

Plasmid

#25911

Depositor

Kenneth Marcu

Article

Stanton et al Nucleic Acids Res. 1982 Oct 11. 10(19):5

Insert

Ig germline gamma-1 switch region alpha

Expression

Bacterial

Available Since

Aug. 11, 2010

Availability

Academic Institutions and Nonprofits only
pAW092

Plasmid

#113250

Purpose

E. coli expression vector for His-MBP-S. pyogenes Cas1 H205A

Depositor

Jennifer Doudna

Article

Wright et al Nat Struct Mol Biol. 2016 Oct;23(10):876

Insert

S. pyogenes Cas1

Tags

6xHis-MBP

Expression

Bacterial

Mutation

H205A

Promoter

T7

Available Since

April 16, 2019

Availability

Academic Institutions and Nonprofits only

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His_Naa80d in pTYB12

Plasmid

#188455

Purpose

Express in E. coli human Naa80 delta (actin's N-terminal acetyl transferase) with N-terminal intein-chitin binding domain tag and 6xHis tag.

Depositor

Roberto Dominguez

Article

Ceron et al Proc Natl Acad Sci U S A. 2022 Oct 11;11

Insert

Naa80 (NAA80 Human)

Tags

intein-CBD, 6xHis

Expression

Bacterial

Mutation

WT

Promoter

T7

Available Since

Nov. 7, 2022

Availability

Academic Institutions and Nonprofits only

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pHisAlix deltaPRD

Plasmid

#42577

Depositor

James Hurley

Article

Carlson et al Proc Natl Acad Sci U S A. 2012 Oct 16;10

Insert

delta-PRD ALIX (residues 1-702) (PDCD6IP Human)

Tags

HIS-TEV

Expression

Bacterial

Mutation

silent mutation A1617G (numbering in ALIX gene)

Available Since

Dec. 19, 2013

Availability

Academic Institutions and Nonprofits only
sg_shuttle_RFP657

Plasmid

#134968

Purpose

Lentiviral vector for sgRNA expression

Depositor

Beat Bornhauser

Article

Huang et al Hemasphere. 2020 Sep 30;4(5):e456. doi:

Type

Empty backbone

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6-sgRNA; EFS-RFP657

Available Since

Feb. 4, 2020

Availability

Academic Institutions and Nonprofits only

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BGGLO QPCR PLASMID

Plasmid

#79905

Purpose

Encodes partial human gamma- and beta-globing cDNA for use as QPCR standard as published in PMID: 14592835..

Depositor

Jeffery Miller

Article

Bhanu et al Blood. 2004 Mar 1;103(5):1929-33. Epub 2

Insert

Human partial HBG and HBB gene

Promoter

No promoter

Available Since

Sept. 6, 2016

Availability

Academic Institutions and Nonprofits only

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pAAV-TRE3g-mNeon-WPRE-pA

Plasmid

#192064

Purpose

mNeonGreen expression with TRE3g promoter

Depositor

Josh Huang

Article

Qian et al Nature. 2022 Oct;610(7933):713-721. doi:

Insert

mNeonGreen

Use

AAV

Expression

Mammalian

Promoter

TRE3g

Available Since

Feb. 2, 2023

Availability

Academic Institutions and Nonprofits only

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pX330-RPA194

Plasmid

#247352

Purpose

Expresses SpCas9 and a sgRNA targeting the human RPA194 loci for knock-in.

Depositor

Kazuhiro Maeshima

Article

Ide et al Sci Adv. 2020 Oct 14;6(42). pii: 6/42/ea

Insert

RPA194 (POLR1A Human)

Use

CRISPR

Promoter

U6

Available Since

Nov. 18, 2025

Availability

Academic Institutions and Nonprofits only

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pX330-UBF

Plasmid

#247350

Purpose

Expresses SpCas9 and a sgRNA targeting the human UBF loci for knock-in.

Depositor

Kazuhiro Maeshima

Article

Ide et al Sci Adv. 2020 Oct 14;6(42). pii: 6/42/ea

Insert

UBF (UBTF Human)

Use

CRISPR

Promoter

U6

Available Since

Nov. 18, 2025

Availability

Academic Institutions and Nonprofits only

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YCplac33-NAT_NDT1

Plasmid

#140593

Purpose

Express NDT1 in S. cerevisiae

Depositor

David Sabatini

Article

Kory et al Sci Adv. 2020 Oct 21;6(43):eabe5310. doi

Insert

NDT1 with 5' and 3' UTRs (YIA6 Budding Yeast)

Expression

Yeast

Promoter

NDT1 promoter and 3'UTR

Available Since

Sept. 11, 2020

Availability

Academic Institutions and Nonprofits only

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pS34.EF1α<GFP-2A-Cas9-RC

Plasmid

#207404

Purpose

Cas9-RC and GFP from an EF1α promoter. High-performance knockin through DSB repair by homology-directed recombination (HDR) or homology-mediated end joining (HMEJ). [Lab plasmid ID: N213]

Depositor

Alexandros Poulopoulos

Article

Richardson et al CRISPR J. 2023 Oct;6(5):447-461. doi: 10

Insert

Cas9-RC

Use

CRISPR and Synthetic Biology

Tags

GFP-2A

Expression

Mammalian

Promoter

EF1α

Available Since

Nov. 3, 2023

Availability

Academic Institutions and Nonprofits only

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pTRIPZ-hDDX5/17

Plasmid

#71307

Purpose

Inducible shRNA knockdown of both human DDX5 and DDX17 genes

Depositor

Allan Brasier

Article

Yang et al Mol Cell Proteomics. 2015 Oct;14(10):270

Insert

DDX5/17 shRNA (DDX17 Human)

Use

Lentiviral and RNAi

Expression

Mammalian

Promoter

TetO

Available Since

Dec. 22, 2015

Availability

Academic Institutions and Nonprofits only

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pRL838

Plasmid

#70680

Purpose

This vector was used to generate a library of ca. 17-kb sequences of clones as part of a genomic sequence strategy and were then used to complement mutations.

Depositor

Peter Wolk

Article

Kaneko et al DNA Res. 2001 Oct 31;8(5):205-13; 227-53

Type

Empty backbone

Use

Cre/Lox

Available Since

Nov. 17, 2015

Availability

Academic Institutions and Nonprofits only

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pExodus CMV.Artemis

Plasmid

#40211

Purpose

Mammalian expression of codon-optimized Artemis.

Depositor

Andrew Scharenberg

Article

Certo et al Nat Methods. 2012 Oct;9(10):973-5. doi:

Insert

Artemis (DCLRE1C Human)

Expression

Bacterial and Mammalian

Promoter

CMV and T7

Available Since

Sept. 27, 2012

Availability

Academic Institutions and Nonprofits only

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EK0438 SFFV-mCherry-SGc(s70587)cSG-EGFP-(3xMS2) (FLP-IN)

Plasmid

#191163

Purpose

Expression of a sensor for synthetic sequence 1 (EK0208) with three MS2 sequences in the 3' UTR in mammalian cells. Compared to EK0285, also has additional restriction sites around the sensor sequence. Recommended for the basis for cloning new sensors for use with MCP-ADAR(DD). HindIII & MluI can be used to swap out marker (mCherry). (KpnI or BamHI) and (BshTI or BcuI) can be used to swap out sensor sequence (s70587). XhoI and ApaI can be used to swap out output (EGFP).

Depositor

Xiaojing Gao

Article

Kaseniit et al Nat Biotechnol. 2022 Oct 5. pii: 10.1038

Insert

mCherry:s70587:EGFP-3xMS2

Expression

Mammalian

Mutation

WT

Promoter

SFFV

Available Since

Oct. 26, 2022

Availability

Academic Institutions and Nonprofits only

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