Addgene

We narrowed to 1,214 results for: 5-Oct

Showing: 621 - 640 of 1214 results

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His-FLAG-Tev_ACTB (beta-actin)

Plasmid

#188453

Purpose

Express in mammalian cells human beta-actin (ACTB) with N-terminal 6xHis tag, FLAG tag, and TEV protease site.

Depositor

Roberto Dominguez

Article

Ceron et al Proc Natl Acad Sci U S A. 2022 Oct 11;11

Insert

ACTB (ACTB Human)

Use

Tags

6xHis-FLAG

Expression

Mammalian

Mutation

WT

Promoter

CMV

Available since

Oct. 28, 2022

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn-ADAR2-sesRNA-tTA2-W3SL

Plasmid

#192068

Purpose

Expression of ADAR2, sesRNA in mammalian cells with hSyn promoter, with tTA2 as efRNA and ADAR2 overexpression.

Depositor

Josh Huang

Article

Qian et al Nature. 2022 Oct;610(7933):713-721. doi:

Insert

sesRNA for mouse Ctip2

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

hSyn

Available since

Jan. 19, 2023

Availability

Academic Institutions and Nonprofits only

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His-FLAG-Tev_ACTA1 (alpha-actin)

Plasmid

#188452

Purpose

Express in mammalian cells human alpha-actin (ACTA1) with N-terminal 6xHis tag, FLAG tag, and TEV protease site.

Depositor

Roberto Dominguez

Article

Ceron et al Proc Natl Acad Sci U S A. 2022 Oct 11;11

Insert

ACTA1 (ACTA1 Human)

Use

Tags

6xHis-FLAG

Expression

Mammalian

Mutation

WT

Promoter

CMV

Available since

Oct. 28, 2022

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn-ClipF-sesRNA-smV5-tTA2-W3SL

Plasmid

#192067

Purpose

Expression of ClipF, sesRNA in mammalian cells, with smV5 and tTA2 as efRNA.

Depositor

Josh Huang

Article

Qian et al Nature. 2022 Oct;610(7933):713-721. doi:

Insert

sesRNA for human FXOP2

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

hSyn

Available since

Jan. 11, 2023

Availability

Academic Institutions and Nonprofits only

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YCplac33-NAT_MCART1K91A

Plasmid

#140592

Purpose

Express MCART1K91A in S. cerevisiae

Depositor

David Sabatini

Article

Kory et al Sci Adv. 2020 Oct 21;6(43):eabe5310. doi

Insert

SLC25A51 with 5' and 3' UTR of scNDT1 (SLC25A51 Human)

Use

Tags

Expression

Yeast

Mutation

codon-optimized for expression in S. cerevisiae; …

Promoter

NDT1 promoter and 3'UTR

Available since

Sept. 11, 2020

Availability

Academic Institutions and Nonprofits only

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Bet1-GFP

Plasmid

#209850

Purpose

Expresses Bet1-GFP in mammalian cells

Depositor

Geert van den Bogaart

Article

Linders et al Nat Commun. 2021 Oct 28;12(1):6227. doi:

Insert

Bet1 (BET1 Human)

Use

Tags

eGFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Nov. 8, 2023

Availability

Academic Institutions and Nonprofits only

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pSpCas9n(BB)-2A-Puro (PX462) V2.0 Stx5DL-B1

Plasmid

#165082

Purpose

gRNA 1 of pair B for Stx5L-specific knockout

Depositor

Geert van den Bogaart

Article

Linders et al Nat Commun. 2021 Oct 28;12(1):6227. doi:

Insert

hSpCas9n-2A-Puro

Use

CRISPR

Tags

2A-Puro and 3XFLAG

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Nov. 18, 2021

Availability

Academic Institutions and Nonprofits only

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Bet1L-mCherry

Plasmid

#209849

Purpose

Expresses Bet1L-mCherry in mammalian cells

Depositor

Geert van den Bogaart

Article

Linders et al Nat Commun. 2021 Oct 28;12(1):6227. doi:

Insert

Bet1L (BET1L Human)

Use

Tags

mCherry

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Nov. 8, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1/ZMYM2-FGFR1

Plasmid

#167486

Purpose

Mammalian expression vector for the ZMYM2-FGFR1 fusion gene associated with myeloid and lymphoid neoplasms

Depositor

Nicholas Cross

Article

Smedley et al Neoplasia. 1999 Oct;1(4):349-55. doi: 10

Insert

ZMYM2-FGFR1 (FGFR1, ZMYM2 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

April 30, 2021

Availability

Academic Institutions and Nonprofits only

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Bet1-mCherry

Plasmid

#209848

Purpose

Expresses Bet1-mCherry in mammalian cells

Depositor

Geert van den Bogaart

Article

Linders et al Nat Commun. 2021 Oct 28;12(1):6227. doi:

Insert

Bet1 (BET1 Human)

Use

Tags

mCherry

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Nov. 8, 2023

Availability

Academic Institutions and Nonprofits only

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pSpCas9n(BB)-2A-Puro (PX462) V2.0 Stx5DL-B2

Plasmid

#165083

Purpose

gRNA 2 of pair B for Stx5L-specific knockout

Depositor

Geert van den Bogaart

Article

Linders et al Nat Commun. 2021 Oct 28;12(1):6227. doi:

Insert

hSpCas9n-2A-Puro

Use

CRISPR

Tags

2A-Puro and 3XFLAG

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Nov. 18, 2021

Availability

Academic Institutions and Nonprofits only

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pSpCas9n(BB)-2A-Puro (PX462) V2.0 Stx5DL-C1

Plasmid

#165085

Purpose

gRNA 1 of pair C for Stx5L-specific knockout

Depositor

Geert van den Bogaart

Article

Linders et al Nat Commun. 2021 Oct 28;12(1):6227. doi:

Insert

hSpCas9n-2A-Puro

Use

CRISPR

Tags

2A-Puro and 3XFLAG

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Nov. 18, 2021

Availability

Academic Institutions and Nonprofits only

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pSpCas9n(BB)-2A-Puro (PX462) V2.0 Stx5DL-C2

Plasmid

#165086

Purpose

gRNA 2 of pair C for Stx5L-specific knockout

Depositor

Geert van den Bogaart

Article

Linders et al Nat Commun. 2021 Oct 28;12(1):6227. doi:

Insert

hSpCas9n-2A-Puro

Use

CRISPR

Tags

2A-Puro and 3XFLAG

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Nov. 18, 2021

Availability

Academic Institutions and Nonprofits only

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pCDNA3.1_3XFlag

Plasmid

#182494

Purpose

Empty plasmid containing Flag tag for C-terminal tagging

Depositor

Paolo Comoglio

Article

Cerqua et al Life Sci Alliance. 2022 May 30;5(10). pi

Insert

3xFlag with 5 Glycolic spacer

Use

Tags

Expression

Mutation

Promoter

Available since

June 16, 2022

Availability

Academic Institutions and Nonprofits only

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pmcherry-LCa

Plasmid

#53972

Purpose

Expresses the light chain of clathrin

Depositor

Tom Kirchhausen

Article

Kural et al Cell Rep. 2012 Nov 29;2(5):1111-9. doi:

Insert

clathrin light chain

Use

Tags

mCherry

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 1, 2014

Availability

Academic Institutions and Nonprofits only
pET-15b-EK_C122S_His5

Plasmid

#49048

Purpose

Expresses the pentahistidine-tagged catalytic domain of bovine enteropeptidase in E. coli

Depositor

Hans Brandstetter

Article

Skala et al J Biotechnol. 2013 Dec;168(4):421-5. doi

Insert

Enteropeptidase, peptidase domain (TMPRSS15 Bovine)

Use

Tags

5xHis

Expression

Bacterial

Mutation

P92R, E185D, C122S

Promoter

T7

Available since

Jan. 14, 2014

Availability

Academic Institutions and Nonprofits only

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Setd3-Flag-pcDNA3.0

Plasmid

#49144

Purpose

mammalian expression of Flag tagged Setd3

Depositor

Jing H Wang

Article

Chen et al Haematologica. 2013 May;98(5):739-43. do

Insert

Setd3 (Setd3 Mouse)

Use

Tags

flag

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Oct. 30, 2013

Availability

Academic Institutions and Nonprofits only
Setd3-Flag-pGEX4t-1

Plasmid

#49145

Purpose

bacterial expression of GST-Setd3-Flag

Depositor

Jing H Wang

Article

Chen et al Haematologica. 2013 May;98(5):739-43. do

Insert

Setd3 (Setd3 Mouse)

Use

Tags

FLAG and GST

Expression

Bacterial

Mutation

Promoter

tac

Available since

Oct. 30, 2013

Availability

Academic Institutions and Nonprofits only

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pT7-EGFP-C1-HsHelz_AG

Plasmid

#148799

Purpose

Mammalian Expression of HsHelz

Depositor

Elisa Izaurralde

Article

Hanet et al Life Sci Alliance. 2019 Sep 30;2(5). pii

Insert

HsHelz (HELZ Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 18, 2021

Availability

Academic Institutions and Nonprofits only

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pT7-MS2-HA-HsHelz_AG

Plasmid

#148805

Purpose

Mammalian Expression of HsHelz

Depositor

Elisa Izaurralde

Article

Hanet et al Life Sci Alliance. 2019 Sep 30;2(5). pii

Insert

HsHelz (HELZ Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 25, 2022

Availability

Academic Institutions and Nonprofits only

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