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pHH0103_NOXA1-1/2

Plasmid

#91320

Purpose

Protein expression and purification of human SH3 domain construct NOXA1-1/2

Depositor

Sachdev Sidhu

Article

Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

Insert

NOXA1-1/2 (NOXA1 Human)

Expression

Bacterial

Available Since

March 19, 2018

Availability

Academic Institutions and Nonprofits only

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pHH0103_SH3TC2-1/2

Plasmid

#91294

Purpose

Protein expression and purification of human SH3 domain construct SH3TC2-1/2

Depositor

Sachdev Sidhu

Article

Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

Insert

SH3TC2-1/2 (SH3TC2 Human)

Expression

Bacterial

Available Since

March 19, 2018

Availability

Academic Institutions and Nonprofits only

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pVY-1 sfGFP-tag1

Plasmid

#194616

Purpose

fluorescent proteins with cationic peptide tags to promote biomolecular condensate formation in E. coli

Depositor

Allie Obermeyer

Article

Yeong et al Nat Commun. 2022 Dec 22;13(1):7882. doi:

Insert

sfGFP-KGGSKKRKKR

Tags

KGGSKKRKKR and MGHHHHHGGA

Expression

Bacterial

Promoter

T7

Available Since

Jan. 26, 2023

Availability

Academic Institutions and Nonprofits only

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Cer(1-158)-beta-1 in pcDNAI/Amp

Plasmid

#55707

Purpose

An amino-terminal fragment of mCerulean was fused to Gbeta1. When co-expressed with a carboxyl terminal CFP fragment fused to a Ggamma subunit, a fluorescent signal s produced.

Depositor

Catherine Berlot

Article

Yost et al Mol Pharmacol. 2007 Oct;72(4):812-25. Ep

Insert

mCer(1-158)-beta 1 (GNB1 Aequorea victoria, Human)

Tags

Cer(1-158) was fused to the amino terminus of Gbe…

Expression

Mammalian

Mutation

GBeta-1 was amplified via PCR, which removed an i…

Promoter

CMV

Available Since

July 30, 2015

Availability

Academic Institutions and Nonprofits only

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pHH0103_NOXO1-1/2

Plasmid

#91499

Purpose

Protein expression and purification of human SH3 domain construct NOXO1-1/2

Depositor

Sachdev Sidhu

Article

Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

Insert

NOXO1-1/2 (NOXO1 Human)

Expression

Bacterial

Available Since

March 19, 2018

Availability

Academic Institutions and Nonprofits only

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pHH0103_SORBS1-1/3

Plasmid

#91547

Purpose

Protein expression and purification of human SH3 domain construct SORBS1-1/3

Depositor

Sachdev Sidhu

Article

Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

Insert

SORBS1-1/3 (SORBS1 Human)

Expression

Bacterial

Available Since

March 19, 2018

Availability

Academic Institutions and Nonprofits only

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pHH0103_SH3KBP1-1/3

Plasmid

#91555

Purpose

Protein expression and purification of human SH3 domain construct SH3KBP1-1/3

Depositor

Sachdev Sidhu

Article

Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

Insert

SH3KBP1-1/3 (SH3KBP1 Human)

Expression

Bacterial

Available Since

March 19, 2018

Availability

Academic Institutions and Nonprofits only

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AAV.CBA.YFP.miR-E_shSrpk3-1

Plasmid

#180400

Purpose

Producing AAV that encodes mouse Srpk3 shRNA-1 with miR-E backbone

Depositor

Huda Zoghbi

Article

Lee et al J Clin Invest. 2022 May 2;132(9). pii: 1

Insert

shSrpk3-1 (Srpk3 Mouse)

Use

AAV and RNAi

Promoter

CBA

Available Since

Nov. 2, 2022

Availability

Academic Institutions and Nonprofits only

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Bmpr2 gRNA#1

Plasmid

#163388

Purpose

Cas9-mediated knockout of Bmpr2 in mammalian cells

Depositor

Takeshi Imai

Article

Aihara et al Cell Rep. 2021 Jun 22;35(12):109276. doi

Insert

Bmpr2 (Bmpr2 Mouse)

Use

CRISPR

Expression

Mammalian

Available Since

Aug. 30, 2021

Availability

Academic Institutions and Nonprofits only

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Grin1 gRNA#1

Plasmid

#169789

Purpose

Cas9-mediated knockout of Grin1 in mammalian cells

Depositor

Takeshi Imai

Article

Aihara et al Cell Rep. 2021 Jun 22;35(12):109276. doi

Insert

Grin1 (Grin1 Mouse)

Use

CRISPR

Expression

Mammalian

Available Since

Aug. 23, 2021

Availability

Academic Institutions and Nonprofits only

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pLenti-sgCTRL-1

Plasmid

#83934

Purpose

Lentiviral vector expressing a control sgRNA. NOTE: This plasmid does NOT contain Cas9.

Depositor

David Sabatini

Article

Park et al Nat Genet. 2016 Dec 19. doi: 10.1038/ng.

Insert

sgCTRL-1

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6

Available Since

Feb. 1, 2021

Availability

Academic Institutions and Nonprofits only

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pX330_gRNA_IG-DMR-1

Plasmid

#159931

Purpose

Specific gRNA against mouse IG-DMR cloned in the pX330 backbone (Addgene Number 42230). Deletion of IG-DMR in mouse ESC.

Depositor

Anton Wutz

Article

Aizawa et al PLoS One. 2020 Sep 10;15(9):e0233072. do

Insert

gRNA mouse IG-DMR

Use

CRISPR and Mouse Targeting

Expression

Mammalian

Promoter

U6, CBh

Available Since

Nov. 17, 2020

Availability

Academic Institutions and Nonprofits only

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pHH0103_BZRAP1-1/3

Plasmid

#91487

Purpose

Protein expression and purification of human SH3 domain construct BZRAP1-1/3

Depositor

Sachdev Sidhu

Article

Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

Insert

BZRAP1-1/3 (TSPOAP1 Human)

Expression

Bacterial

Available Since

March 19, 2018

Availability

Academic Institutions and Nonprofits only

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pHH0103_NCF2-1/2

Plasmid

#91479

Purpose

Protein expression and purification of human SH3 domain construct NCF2-1/2

Depositor

Sachdev Sidhu

Article

Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

Insert

NCF2-1/2 (NCF2 Human)

Expression

Bacterial

Available Since

March 19, 2018

Availability

Academic Institutions and Nonprofits only

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pHH0103_GRAP-1/2

Plasmid

#91412

Purpose

Protein expression and purification of human SH3 domain construct GRAP-1/2

Depositor

Sachdev Sidhu

Article

Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

Insert

GRAP-1/2 (GRAP Human)

Expression

Bacterial

Available Since

March 19, 2018

Availability

Academic Institutions and Nonprofits only

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pHH0103_RIMBP2-1/3

Plasmid

#91388

Purpose

Protein expression and purification of human SH3 domain construct RIMBP2-1/3

Depositor

Sachdev Sidhu

Article

Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

Insert

RIMBP2-1/3 (RIMBP2 Human)

Expression

Bacterial

Available Since

March 19, 2018

Availability

Academic Institutions and Nonprofits only

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pHH0103_SH3PXD2B-1/4

Plasmid

#91317

Purpose

Protein expression and purification of human SH3 domain construct SH3PXD2B-1/4

Depositor

Sachdev Sidhu

Article

Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

Insert

SH3PXD2B-1/4 (SH3PXD2B Human)

Expression

Bacterial

Available Since

March 19, 2018

Availability

Academic Institutions and Nonprofits only

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pHH0103_RIMBP3-1/3

Plasmid

#91331

Purpose

Protein expression and purification of human SH3 domain construct RIMBP3-1/3

Depositor

Sachdev Sidhu

Article

Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

Insert

RIMBP3-1/3 (RIMBP3 Human)

Expression

Bacterial

Available Since

March 19, 2018

Availability

Academic Institutions and Nonprofits only

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pHH0103_NCF1-1/2

Plasmid

#91305

Purpose

Protein expression and purification of human SH3 domain construct NCF1-1/2

Depositor

Sachdev Sidhu

Article

Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

Insert

NCF1-1/2 (NCF1 Human)

Expression

Bacterial

Available Since

March 19, 2018

Availability

Academic Institutions and Nonprofits only

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plko.1/shMCT4 #474

Plasmid

#99597

Purpose

Knockdown of MCT4 expression

Depositor

Eli Bar

Article

Lim et al Oncogene. 2014 Aug 28;33(35):4433-41. do

Insert

MCT4 (SLC16A3 Human)

Use

Lentiviral

Available Since

Oct. 16, 2017

Availability

Academic Institutions and Nonprofits only

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