We narrowed to 1,008 results for: nanobody

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pET29b_VHH21-Cys-His

Plasmid

#233325

Purpose

To express nanobody VHH21 with C terminal cysteine

Depositor

Magnus Rueping

Publication

Gutu et al Curr Protoc. 2025 Oct;5(10):e70213. doi:

Insert

VHH21-Cys-His

Expression

Bacterial

Promoter

T7

Available since

Oct. 14, 2025

Availability

Academic Institutions and Nonprofits only

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pET29b_IGHJ4_1-Cys-His

Plasmid

#233326

Purpose

To express nanobody IGHJ4_1 with C terminal cysteine

Depositor

Magnus Rueping

Publication

Gutu et al Curr Protoc. 2025 Oct;5(10):e70213. doi:

Insert

IGHJ4_1-Cys-His

Expression

Bacterial

Promoter

T7

Available since

Oct. 14, 2025

Availability

Academic Institutions and Nonprofits only

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pET29b_IGHJ4_2-Cys-His

Plasmid

#233327

Purpose

To express nanobody IGHJ4_2 with C terminal cysteine

Depositor

Magnus Rueping

Publication

Gutu et al Curr Protoc. 2025 Oct;5(10):e70213. doi:

Insert

IGHJ4_2-Cys-His

Expression

Bacterial

Promoter

T7

Available since

Oct. 14, 2025

Availability

Academic Institutions and Nonprofits only

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pET29b_IGHJ4_3-Cys-His

Plasmid

#233328

Purpose

To express nanobody IGHJ4_3 with C terminal cysteine

Depositor

Magnus Rueping

Publication

Gutu et al Curr Protoc. 2025 Oct;5(10):e70213. doi:

Insert

IGHJ4_3-Cys-His

Expression

Bacterial

Promoter

T7

Available since

Oct. 14, 2025

Availability

Academic Institutions and Nonprofits only

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pET29b_IGHJ4_4-Cys-His

Plasmid

#233329

Purpose

To express nanobody IGHJ4_4 with C terminal cysteine

Depositor

Magnus Rueping

Publication

Gutu et al Curr Protoc. 2025 Oct;5(10):e70213. doi:

Insert

IGHJ4_4-Cys-His

Expression

Bacterial

Promoter

T7

Available since

Oct. 14, 2025

Availability

Academic Institutions and Nonprofits only

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pET29b_IGHJ5-Cys-His

Plasmid

#233330

Purpose

To express nanobody IGHJ5 with C terminal cysteine

Depositor

Magnus Rueping

Publication

Gutu et al Curr Protoc. 2025 Oct;5(10):e70213. doi:

Insert

IGHJ5-Cys-His

Expression

Bacterial

Promoter

T7

Available since

Oct. 14, 2025

Availability

Academic Institutions and Nonprofits only

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pET29b_SpyTag003-Linker-C6-His

Plasmid

#233331

Purpose

To express nanobody C6 with N terminal SpyTag

Depositor

Magnus Rueping

Publication

Gutu et al Curr Protoc. 2025 Oct;5(10):e70213. doi:

Insert

SpyTag003-Linker-C6-His

Expression

Bacterial

Promoter

T7

Available since

Oct. 14, 2025

Availability

Academic Institutions and Nonprofits only

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pET29b_SpyTag003-L-VHH16-His

Plasmid

#233332

Purpose

To express nanobody VHH16 with N terminal SpyTag

Depositor

Magnus Rueping

Publication

Gutu et al Curr Protoc. 2025 Oct;5(10):e70213. doi:

Insert

SpyTag003-L-VHH16-His

Expression

Bacterial

Promoter

T7

Available since

Oct. 14, 2025

Availability

Academic Institutions and Nonprofits only

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pET29b_SpyTag003-L-IGHJ4_1-His

Plasmid

#233334

Purpose

To express nanobody IGHJ4_1 with N terminal SpyTag

Depositor

Magnus Rueping

Publication

Gutu et al Curr Protoc. 2025 Oct;5(10):e70213. doi:

Insert

SpyTag003-L-IGHJ4_1-His

Expression

Bacterial

Promoter

T7

Available since

Oct. 14, 2025

Availability

Academic Institutions and Nonprofits only

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pTS103 pHAGE2 CMVtetO2 MCS-22xGS-GFP

Plasmid

#199356

Purpose

Lentiviral transfer plasmid for doxycycline inducible expression of a C-terminally GFP-tagged protein in mammalian cells.

Depositor

Rebecca Voorhees

Publication

Stevens et al Nat Protoc. 2024 Jan;19(1):127-158. doi:

Type

Empty backbone

Use

Lentiviral

Tags

EGFP

Expression

Mammalian

Mutation

WT

Available since

April 21, 2023

Availability

Academic Institutions and Nonprofits only

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pTS110 pHAGE2 CMVtetO2 MCS-22xGS-Alfa-P2A-TagBFP

Plasmid

#199363

Purpose

Lentiviral transfer plasmid for doxycycline inducible expression of a C-terminally ALFA-P2A-BFP-tagged protein in mammalian cells.

Depositor

Rebecca Voorhees

Publication

Stevens et al Nat Protoc. 2024 Jan;19(1):127-158. doi:

Type

Empty backbone

Use

Lentiviral

Tags

ALFA-P2A-TagBFP

Expression

Mammalian

Mutation

WT

Available since

April 21, 2023

Availability

Academic Institutions and Nonprofits only

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pTS100 pHAGE2 CMVtetO2 TagBFP-P2A-ALFA-22xGS-TEV-MCS

Plasmid

#199353

Purpose

Lentiviral transfer plasmid for doxycycline inducible expression of a N-terminally BFP-P2A-ALFA-TEV-tagged protein in mammalian cells.

Depositor

Rebecca Voorhees

Publication

Stevens et al Nat Protoc. 2024 Jan;19(1):127-158. doi:

Type

Empty backbone

Use

Lentiviral

Tags

TagBFP-P2A-ALFA-TEV

Expression

Mammalian

Mutation

WT

Available since

May 23, 2023

Availability

Academic Institutions and Nonprofits only

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pAW-CD8-Nb127D01-GFP

Plasmid

#171577

Purpose

Expresses GFP-tagged anti-CXCR2 (human) recombinant llama nanobody (Nb127D01-GFP) in fly cell cytomembrane

Depositor

Norbert Perrimon, Hidde Ploegh

Publication

Xu et al Elife. 2022 Jan 25;11. pii: 74326. doi:

Insert

CD8-Nb127D01-GFP (CXCR2 Human)

Tags

CD8 and GFP

Expression

Insect

Promoter

fly actin5C promoter

Available since

June 30, 2021

Availability

Academic Institutions and Nonprofits only

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pAJF1137

Plasmid

#252907

Purpose

Tet-inducible TurboID-NBALFA fusion plasmid. Contains oriM (mycobacterial origin of replication) and kanamycin-resistance cassette.

Depositor

Michael Glickman

Publication

Fay et al mBio. 2025 Aug 13;16(8):e0097125. doi: 1

Insert

TurboID

Expression

Bacterial

Promoter

Tet-inducible promoter

Available since

March 30, 2026

Availability

Academic Institutions and Nonprofits only

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pOPINVHH_BAP_his

Plasmid

#210402

Purpose

For expression of VHH with C-terminal biotin acceptor peptide and his tag

Depositor

Ray Owens

Publication

Eyssen et al Bio Protoc. 2024 Mar 20;14(6):e4962. doi

Type

Empty backbone

Tags

biotin acceptor peptide-his6

Expression

Bacterial

Promoter

lac

Available since

March 26, 2024

Availability

Academic Institutions and Nonprofits only

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pSB_init-Sb66

Plasmid

#159422

Purpose

Expression of the anti-TGP (thermostable GFP) synthetic nanobody Sb66

Depositor

Dianfan Li

Publication

Cai et al Commun Biol. 2020 Dec 10;3(1):753. doi:

Insert

Sb66

Tags

c-Myc and poly-His

Expression

Bacterial

Available since

April 12, 2021

Availability

Academic Institutions and Nonprofits only

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pmCherry-VIM-BC2T

Plasmid

#108966

Purpose

expresses mCherry-vimentin-BC2-tag in mammalian cells

Depositor

Ulrich Rothbauer

Publication

Virant et al Nat Commun. 2018 Mar 2;9(1):930. doi: 10

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

VIM (VIM Human)

Tags

BC2T and mCherry

Expression

Mammalian

Available since

June 22, 2018

Availability

Academic Institutions and Nonprofits only

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pVIM-BC2T

Plasmid

#108967

Purpose

expresses vimentin-BC2-tag in mammalian cells

Depositor

Ulrich Rothbauer

Publication

Virant et al Nat Commun. 2018 Mar 2;9(1):930. doi: 10

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

Vimentin

Tags

BC2T

Expression

Mammalian

Available since

June 22, 2018

Availability

Academic Institutions and Nonprofits only

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pOPINO_H3

Plasmid

#171927

Purpose

Bacterial expression of nanobody VHH_H3, which binds to receptor binding domain of the spike protein of SARS-CoV-2

Depositor

Ray Owens

Publication

Huo et al Nat Commun. 2021 Sep 22;12(1):5469. doi:

Insert

VHH_H3

Tags

His6

Expression

Bacterial and Mammalian

Available since

Sept. 23, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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hIgG1-Ty1-hFc

Plasmid

#194593

Purpose

Mammalian expression of Ty1 nanobody upstream of human IgG1

Depositor

Gerald McInerney

Publication

Hanke et al Nat Commun. 2020 Sep 4;11(1):4420. doi:

Insert

Ty1-hFc (S )

Expression

Mammalian

Available since

Feb. 1, 2023

Availability

Academic Institutions and Nonprofits only

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