We narrowed to 6,522 results for: poly

1. PABPC1_544-626_pGEX6P1

   Plasmid

   #209790

   Purpose

   Bacterial expression of PABPC1 Mlle domain

   Depositor

   Kalle Gehring

   Article

   Kozlov et al J Biol Chem. 2010 Apr 30;285(18):13599-6

   Insert

   Mlle domain of PABPC1 (PABPC1 Human)
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   aa 544-626 only

   Promoter

   tac

   Available since

   Nov. 10, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. mCherry-cGAS-HA

   Plasmid

   #231895

   Purpose

   Expression of cyclic GMP/AMP synthase (cGAS), as a nuclear rupture marker

   Depositor

   Lukas Kapitein

   Article

   Korsten et al J Cell Biol. 2024 Nov 4;223(11):e2023071

   Insert

   cGAS (CGAS Human)
   

   Use

   Tags

   3xHA and mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 11, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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3. emiRFP670-cGAS

   Plasmid

   #231896

   Purpose

   Expression of cyclic GMP/AMP synthase (cGAS), as a nuclear rupture marker

   Depositor

   Lukas Kapitein

   Article

   Korsten et al J Cell Biol. 2024 Nov 4;223(11):e2023071

   Insert

   cGAS (CGAS Human)
   

   Use

   Tags

   emiRFP670

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 11, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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4. pGEX6P-2-human RNF146 full length

   Plasmid

   #132610

   Purpose

   expresses human RNF146 full length in E. coli

   Depositor

   Wenqing Xu

   Article

   DaRosa et al Nature. 2015 Jan 8;517(7533):223-6. doi:

   Insert

   RNF146 (RNF146 Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Oct. 17, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pIRES-Puro-MGMT

   Plasmid

   #187654

   Purpose

   Expresses hMGMT together with the puromycin resistance gene via an IRES.

   Depositor

   Robert Sobol

   Article

   Li et al Cancers (Basel). 2022 Jul 22;14(15). pii

   Insert

   MGMT (MGMT Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Sept. 14, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pPTG-GG-sg2+sg3

   Plasmid

   #127199

   Purpose

   Plasmid expressing sgRNA2 (TACCACATTTGTAGAGGTT) & sgRNA3 (CAATGTATCTTATCATGTC) as polycistronic tRNA-gRNA from single U6 promoter

   Depositor

   Jordan Green

   Article

   Rui et al Mol Ther Nucleic Acids. 2020 Apr 21;20:6

   Insert

   tRNA-gRNA
   

   Use

   Empty sgrna plasmid

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   May 28, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. Ubc-c005

   Plasmid

   #216876

   Purpose

   Bacterial expression vector for E. coli producing ubiquitin with N-terminal YFP fusion

   Depositor

   Lari Lehtiö

   Article

   Vela-Rodriguez et al SLAS Discov. 2023 Dec;28(8):365-375. doi

   Insert

   Ubiquitin (UBC Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   May 23, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. Ubc-c004

   Plasmid

   #216875

   Purpose

   Bacterial expression vector for E. coli producing ubiquitin with N-terminal CFP fusion

   Depositor

   Lari Lehtiö

   Article

   Vela-Rodriguez et al SLAS Discov. 2023 Dec;28(8):365-375. doi

   Insert

   Ubiquitin (UBC Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   May 23, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCMV-PARP1-3xFlag-7xKQ

   Plasmid

   #111576

   Purpose

   Expresses full length PARP1 with K to Q mutations in automodification domain in mammalian cells

   Depositor

   Thomas Muir

   Article

   Liszczak et al Nat Chem Biol. 2018 Jul 16. pii: 10.1038

   Insert

   PARP1 (PARP1 Human)
   

   Use

   Tags

   3xFlag

   Expression

   Mammalian

   Mutation

   lysine to glutamine mutation at the following ami…

   Promoter

   CMV

   Available since

   July 18, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCI-MS2V5-eRF3a F76a

    Plasmid

    #65811

    Purpose

    expression of eRF3a-MS2 fusion. F76A mutation abolishes binding to PABPC1

    Depositor

    Niels Gehring

    Article

    Fatscher et al RNA. 2014 Oct;20(10):1579-92. doi: 10.12

    Insert

    eRF3a (GSPT1 Human)
    

    Use

    Tags

    MS2 stem loops and V5

    Expression

    Mammalian

    Mutation

    F76a

    Promoter

    CMV

    Available since

    Sept. 17, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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11. pAG32-3HA-3FLAG

    Plasmid

    #154081

    Purpose

    For C-terminal 3xHA-3xFLAG-protein tagging in S. pombe using hphMX4 as resistance marker.

    Depositor

    Cornelia Kilchert

    Article

    Kilchert et al Genome Res. 2020 Jul;30(7):1012-1026. do

    Insert

    3xHA-3xFLAG-hygR
    

    Use

    Pcr-based tagging in yeast

    Tags

    3xHA-3xFLAG

    Expression

    Mutation

    Promoter

    Available since

    Sept. 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. pMVQA

    Plasmid

    #197894

    Purpose

    pCR®2.1-TOPO® backbone with 3' end of HIV-1(HXB2:9045 till poly-A (20))

    Depositor

    Thomas Klimkait

    Article

    Otte et al Cell Rep Methods. 2023 May 23;3(6):10048

    Insert

    HIV-1 3' end (HXB2: 9045 till poly-A(10)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    June 7, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pENTR4 Ta-SRO1 JN177

    Plasmid

    #192546

    Purpose

    pENTR plasmid Triticum aestivum SRO1, full CDS, cultivar JN177, no stop codon

    Depositor

    Lennart Wirthmueller

    Article

    Vogt et al Plant Cell. 2022 Aug 18. pii: 6671231. d

    Insert

    SIMILAR TO RCD ONE 1 (SRO1), cultivar Jinan 177 (JN177)
    

    Use

    Gateway entry vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 2, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pENTR4 Ta-sro1 SR3

    Plasmid

    #192547

    Purpose

    pENTR plasmid Triticum aestivum sro1, full CDS, cultivar SR3, no stop codon

    Depositor

    Lennart Wirthmueller

    Article

    Vogt et al Plant Cell. 2022 Aug 18. pii: 6671231. d

    Insert

    SIMILAR TO RCD ONE 1 (SRO1), cultivar Shanrong No. 3 (SR3)
    

    Use

    Gateway entry vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 2, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. HaloTag-LaminA

    Plasmid

    #231894

    Purpose

    Expression of HaloTag-tagged LaminA to visualize the nuclear lamina

    Depositor

    Lukas Kapitein

    Article

    Korsten et al J Cell Biol. 2024 Nov 4;223(11):e2023071

    Insert

    LaminA (LMNA Human)
    

    Use

    Tags

    HaloTag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 20, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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16. pOPINE GFP nanobody:Halo:His6

    Plasmid

    #111090

    Purpose

    Bacterial expression of a fusion protein consisting of a GFP nanobody (Dr. Brett Collins, Addgene plasmid # 49172), the Halo tag and a His6 tag.

    Depositor

    Lennart Wirthmueller

    Article

    Chen et al Front Plant Sci. 2018 Nov 1;9:1581. doi:

    Insert

    GFP Nanobody
    

    Use

    Tags

    Halo and His6

    Expression

    Bacterial, Insect, and Mamm…

    Mutation

    Promoter

    T7 promoter

    Available since

    June 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. PX458 V3

    Plasmid

    #226957

    Purpose

    CBh-SpCas9-2A-GFP, and hU6-sgRNA (Sp) with a BbsI golden gate cloning backbone for sgRNA. sgRNA uses 3TC scaffold for increased sgRNA expression.

    Depositor

    Paul Thomas

    Article

    Chey et al BMC Genomics. 2025 Feb 12;26(1):138. doi

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 30, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. PARP1 FL

    Plasmid

    #169815

    Purpose

    Expresses codon optimised full length His tagged PARP1 in bacterial cells

    Depositor

    David Neuhaus

    Article

    Ogden et al Nucleic Acids Res. 2021 Feb 26;49(4):226

    Insert

    Poly[ADP-ribose] polymerase 1 (PARP1 Codon optimised, Human, Synthetic)
    

    Use

    Tags

    MKHHHHHHMKQ

    Expression

    Bacterial

    Mutation

    Valine 762 mutated to an alanine

    Promoter

    T7 promoter

    Available since

    Aug. 26, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. PDG459 V3

    Plasmid

    #226958

    Purpose

    CBh-SpCas9-2A-Puro, and 2X hU6-sgRNA (Sp) with BbsI golden gate cloning backbone dual gRNAs. sgRNA uses 3TC scaffold for increased sgRNA expression.

    Depositor

    Paul Thomas

    Article

    Chey et al BMC Genomics. 2025 Feb 12;26(1):138. doi

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 30, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. ABEmax-Puro V3

    Plasmid

    #226956

    Purpose

    ABEmax plasmid enabling A:T to G:C base editing using a single plasmid. sgRNA uses 3TC scaffold for increased sgRNA expression.

    Depositor

    Paul Thomas

    Article

    Chey et al BMC Genomics. 2025 Feb 12;26(1):138. doi

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 10, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge