We narrowed to 10,650 results for: t7

1. pET-OleD

   Plasmid

   #59373

   Purpose

   Inducible expression of OleD (DQ195536) under T7 promoter

   Depositor

   Christopher Anderson

   Insert

   OleD
   

   Expression

   Bacterial

   Promoter

   T7 promoter

   Available Since

   Sept. 17, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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2. pET24a-VHH-tev-std

   Plasmid

   #109423

   Purpose

   Bacterial expression of a functionalized anti-GFP (VHH) nanobody with a TEV site. VHH-tev-std is flanked by an N-terminal T7 epitope, and a C-terminal cassette composed of HA, BAP and a His6 epitope

   Depositor

   Martin Spiess

   Article

   Buser et al Proc Natl Acad Sci U S A. 2018 Jul 3;115

   Insert

   anti-GFP nanobody fused to a T7, TEV cleavage site, HA, BAP and His6 epitope
   

   Tags

   BAP, HA, His6, and T7

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   June 19, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. pET24a-VHH-tev-2xTS

   Plasmid

   #117750

   Purpose

   Bacterial expression of a functionalized anti-GFP (VHH) nanobody fused to two tyrosine sulfation (TS) motifs. VHH-2xTS also contains a T7, HA, BAP, tev cleavage site and His6 epitope

   Depositor

   Martin Spiess

   Article

   Buser et al J Vis Exp. 2019 Feb 21;(144). doi: 10.37

   Insert

   anti-GFP nanobody fused to a T7, TS, HA, BAP and His6 epitope
   

   Tags

   BAP, HA, His6, T7, and Tyrosine sulfation (TS) se…

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Feb. 27, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. EcoFlex MoClo Kit

   Plasmid Kit

   #1000000080

   Purpose

   Golden Gate kit for E. coli synthetic biology; consists of a modular library that uses MoClo assembly standard for a variety of uses, e.g. recombinant protein purification, cell-free circuit design

   Depositor

   Paul Freemont

   Available Since

   July 6, 2016

   Availability

   Academic Institutions and Nonprofits only

5. pTNT-pT7-tetR-mCherry

   Plasmid

   #140870

   Purpose

   T7 promoter driven synthesis of TetR-mCherry fusion protein

   Depositor

   Neal Devaraj

   Article

   Niederholtmeyer et al Nat Commun. 2018 Nov 28;9(1):5027. doi:

   Insert

   TetR-mCherry expressed with T7 promoter
   

   Use

   Synthetic Biology

   Tags

   mCherry

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   May 7, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. p6-8

   Plasmid

   #118089

   Purpose

   rapa-T7- mRNA(GFP)

   Depositor

   Bryan Dickinson

   Article

   Pu et al Nat Chem Biol. 2017 Apr;13(4):432-438. d

   Insert

   Rapamycin-inducible split T7 RNAP with T7 driven mRNA for GFP
   

   Expression

   Mammalian

   Available Since

   Nov. 7, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. pAMB_CAT-FspI

   Plasmid

   #61002

   Purpose

   T7-transcription template for CAT control mRNA used for in vitro translation

   Depositor

   Nils Walter

   Article

   Rinaldi et al Nat Commun. 2016 Jan 19;7:8976. doi: 10.

   Insert

   Chloramphenicol acetyltransferase
   

   Use

   Unspecified

   Mutation

   FspI site introduced after T7 terminator

   Promoter

   T7

   Available Since

   Jan. 20, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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8. pOpen-T7gene1

   Plasmid

   #165505

   Purpose

   Highly processive DNA-dependent RNA polymerase derived from T7 bacteriophage

   Depositor

   Drew Endy, Jennifer Molloy, FreeGenes Project

   Insert

   T7 RNA Polymerase
   

   Use

   Synthetic Biology

   Available Since

   July 27, 2021

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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9. pUC19-gRNA

   Plasmid

   #137776

   Purpose

   Contains the T7 promoter and gRNA scaffold and is used for the in vitro transcription of gRNAs

   Depositor

   Kabin Xie

   Insert

   gRNA scaffold
   

   Use

   CRISPR

   Mutation

   pUC19 BsaI destroyed

   Promoter

   T7

   Available Since

   Feb. 14, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCDF-Frb-v1

    Plasmid

    #201923

    Purpose

    E. coli vector expressing FrbA, FrbB, FrbC, FrbD and FrbE for the biosynthesis of non-hydrolyzable phosphoserine from phosphoenolpyruvate.

    Depositor

    Ryan Mehl, Richard Cooley

    Article

    Zhu et al ACS Cent Sci. 2023 Apr 10;9(4):816-835.

    Inserts

    FrbA
    FrbB
    FrbC
    FrbD
    FrbE
    

    Expression

    Bacterial

    Promoter

    T7 (modified), T7 (modified, moderate), T7 (modif…

    Available Since

    June 7, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pETM6-G6-vioABE

    Plasmid

    #73438

    Purpose

    Prodeoxyviolacein pathway (vioABE) in monocistronic configuration, transcriptionally driven by orthogonal T7-lac promoter variant G6.

    Depositor

    Mattheos Koffas

    Article

    Cress et al Nucleic Acids Res. 2016 Apr 13. pii: gkw

    Insert

    PG6-vioABE
    

    Use

    CRISPR and Synthetic Biology

    Expression

    Bacterial

    Promoter

    PG6 (orthogonal T7-lac variant)

    Available Since

    March 1, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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12. pET24a-VHH-2xTS

    Plasmid

    #109419

    Purpose

    Bacterial expression of a functionalized anti-GFP (VHH) nanobody fused to two tyrosine sulfation (TS) motifs. VHH-2xTS also contains a T7, HA, BAP and His6 epitope

    Depositor

    Martin Spiess

    Article

    Buser et al Proc Natl Acad Sci U S A. 2018 Jul 3;115

    Insert

    anti-GFP nanobody fused to a T7, TS, HA, BAP and His6 epitope
    

    Tags

    BAP, HA, His6, T7, and Tyrosine sulfation (TS) se…

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    June 19, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. Polymerase expression - pCMV-Phi29(+exo) (LM2759)

    Plasmid

    #208964

    Purpose

    Unfused Phi29 DNA polymerase (+exo), expressed from CMV or T7 promoters.

    Depositor

    Benjamin Kleinstiver

    Article

    Ferreira da Silva et al Nat Biotechnol. 2024 Jul 22. doi: 10.103

    Insert

    Phi29(+exo)-BPNLS
    

    Use

    CRISPR; In vitro transcription; t7 promoter

    Tags

    BPNLS

    Expression

    Mammalian

    Promoter

    CMV and T7

    Available Since

    Nov. 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. Polymerase expression - pCMV-Phi29 (LM2708)

    Plasmid

    #208965

    Purpose

    Unfused Phi29 DNA polymerase (-exo), expressed from CMV or T7 promoters.

    Depositor

    Benjamin Kleinstiver

    Article

    Ferreira da Silva et al Nat Biotechnol. 2024 Jul 22. doi: 10.103

    Insert

    Phi29-BPNLS
    

    Use

    CRISPR; In vitro transcription; t7 promoter

    Tags

    BPNLS

    Expression

    Mammalian

    Mutation

    Phi29(-exo;D169A)

    Promoter

    CMV and T7

    Available Since

    Nov. 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. Polymerase expression - pCMV-B103 (LM2907)

    Plasmid

    #208968

    Purpose

    Unfused B103 DNA polymerase, expressed from CMV or T7 promoters.

    Depositor

    Benjamin Kleinstiver

    Article

    Ferreira da Silva et al Nat Biotechnol. 2024 Jul 22. doi: 10.103

    Insert

    B103-BPNLS
    

    Use

    CRISPR; In vitro transcription; t7 promoter

    Tags

    BPNLS

    Expression

    Mammalian

    Promoter

    CMV and T7

    Available Since

    Nov. 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. pET24a-VHH-1xTS

    Plasmid

    #109418

    Purpose

    Bacterial expression of a functionalized anti-GFP (VHH) nanobody fused to one tyrosine sulfation (TS) motif. VHH-1xTS also contains a T7, HA, BAP and His6 epitope

    Depositor

    Martin Spiess

    Article

    Buser et al Proc Natl Acad Sci U S A. 2018 Jul 3;115

    Insert

    anti-GFP nanobody fused to a T7, TS, HA, BAP and His6 epitope
    

    Tags

    BAP, HA, His6, T7, and Tyrosine sulfation (TS) se…

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    June 19, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. pSm-Nuclease

    Plasmid

    #132431

    Purpose

    Expresses Serratia marcescens nuclease in E. coli using T7 promoter

    Depositor

    Mark Arbing

    Insert

    Serratia marcescens nuclease (codon optimized)
    

    Tags

    None

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Oct. 7, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. T7RNAP Cyslight A188C

    Plasmid

    #205083

    Purpose

    T7RNAP protein expression

    Depositor

    Smita Patel

    Insert

    T7RNAP Cyslight A188C (T7p07 Bacteriophage T7)
    

    Expression

    Bacterial

    Mutation

    C125S, C347S, C510S, C515S, C530S, C723S N88C

    Available Since

    Nov. 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. T7RNAP Cyslight V597C

    Plasmid

    #205080

    Purpose

    T7RNAP protein expression

    Depositor

    Smita Patel

    Insert

    T7RNAP Cyslight V597C (T7p07 Bacteriophage T7)
    

    Expression

    Bacterial

    Mutation

    C125S, C347S, C510S, C515S, C530S, C723S V597C

    Available Since

    Aug. 23, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. T7RNAP Cyslight V166C

    Plasmid

    #205079

    Purpose

    T7RNAP protein expression

    Depositor

    Smita Patel

    Insert

    T7RNAP Cyslight V166C (T7p07 Bacteriophage T7)
    

    Expression

    Bacterial

    Mutation

    C125S, C347S, C510S, C515S, C530S, C723S V166C

    Available Since

    Aug. 23, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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