We narrowed to 32,661 results for: Eng

1. pML384_NCLN-C-term_XTEN80-mEGFP-3xHA_pTMPLT

   Plasmid

   #227897

   Purpose

   Homology repair template for genome engineering. Organelle endogenous marker expressing GFP and epitope tags for mass spectrometry.

   Depositor

   Manuel Leonetti

   Article

   Hein et al Cell. 2024 Dec 26:S0092-8674(24)01344-8.

   Insert

   NCLN homology arms with mEGFP-3xHA for insertion at C terminus
   

   Use

   CRISPR

   Available Since

   Jan. 16, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. pML318_SEC23A-N-term_XTEN80-mEGFP-3xHA_pTMPLT

   Plasmid

   #227903

   Purpose

   Homology repair template for genome engineering. Organelle endogenous marker expressing GFP and epitope tags for mass spectrometry.

   Depositor

   Manuel Leonetti

   Article

   Hein et al Cell. 2024 Dec 26:S0092-8674(24)01344-8.

   Insert

   SEC23A homology arms with XTEN80-mEGFP-3xHA for insertion at N terminus
   

   Use

   CRISPR

   Available Since

   Jan. 16, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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3. pLJM1-NRBP1-FL-N-FLAG

   Plasmid

   #222675

   Purpose

   overexpressing full length NRBP1 with N-terminal FLAG

   Depositor

   Jason Moffat

   Article

   Xiao et al Cell Rep. 2024 Jul 23;43(7):114417. doi:

   Insert

   NRBP1 (NRBP1 Human)
   

   Use

   Lentiviral

   Tags

   FLAG

   Expression

   Mammalian

   Available Since

   Oct. 18, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pLJM1-TSC22D2-FL-N-FLAG

   Plasmid

   #222680

   Purpose

   overexpressing full length TSC22D2 with N-terminal FLAG

   Depositor

   Jason Moffat

   Article

   Xiao et al Cell Rep. 2024 Jul 23;43(7):114417. doi:

   Insert

   TSC22D2 (TSC22D2 Human)
   

   Use

   Lentiviral

   Tags

   FLAG

   Expression

   Mammalian

   Available Since

   Oct. 18, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pLJM1-TSC22D2-FL-C-FLAG

   Plasmid

   #222681

   Purpose

   overexpressing full length TSC22D2 with C-terminal FLAG

   Depositor

   Jason Moffat

   Article

   Xiao et al Cell Rep. 2024 Jul 23;43(7):114417. doi:

   Insert

   TSC22D2 (TSC22D2 Human)
   

   Use

   Lentiviral

   Tags

   FLAG

   Expression

   Mammalian

   Available Since

   Oct. 18, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pVRC8400-SARS-CoV-2-RBD-BJ.1-AVI

   Plasmid

   #196617

   Purpose

   Expresses SARS-CoV-2 RBD protein from the BJ.1 variant with single chain Fc, HRV3C protease cleavage site, and AVI tag

   Depositor

   Peter Kwong

   Article

   Teng et al PLoS One. 2022 May 24;17(5):e0268767. do

   Insert

   SARS-CoV-2-RBD-BJ.1
   

   Tags

   AVI, HRV3C, and scFc

   Expression

   Mammalian

   Mutation

   G339H, R346T, L368I, S371F, S373P, S375F, T376A, …

   Available Since

   Feb. 21, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pT2iApowriter2(#336)

   Plasmid

   #184104

   Purpose

   cyclofen-inducible apoptosis activation and imaging (tEosFP) in zebrafish transient transgenic

   Depositor

   Sophie Vriz

   Article

   Feng et al Rep Prog Phys. 2013 Jul;76(7):072601. do

   Insert

   myr-DIAP1-5myc-tEosFP-nls'-P2A-Casp9-ERT2
   

   Use

   Zebrafish transient transgenesis

   Tags

   internal tag: 5myc

   Mutation

   DIAP1: deleted 1-2, N19G, N20V, deleted 146-438; …

   Promoter

   Zf-Ubi

   Available Since

   June 9, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pT22Apowriter2(#340)

   Plasmid

   #184105

   Purpose

   cyclofen-inducible apoptosis activation and imaging (tEosFP) in zebrafish permanent transgenic

   Depositor

   Sophie Vriz

   Article

   Feng et al Rep Prog Phys. 2013 Jul;76(7):072601. do

   Insert

   myr-DIAP1-5myc-tEosFP-nls'-P2A-Casp9-ERT2
   

   Use

   Zebrafish transgenesis (blue eyes)

   Tags

   internal tag: 5myc

   Mutation

   DIAP1: deleted 1-2, N19G, N20V, deleted 146-438; …

   Promoter

   Zf-Ubi

   Available Since

   June 9, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pT22iuasvApoWtEos3N(#486)

   Plasmid

   #184107

   Purpose

   cyclofen-inducible apoptosis activation and imaging (tEosFP) in zebrafish permanent transgenic

   Depositor

   Sophie Vriz

   Article

   Feng et al Rep Prog Phys. 2013 Jul;76(7):072601. do

   Insert

   myr-DIAP1-2nls-tEosFP-nls'-P2A-Casp9-ERT2
   

   Use

   Zebrafish transgenesis (blue eyes)

   Mutation

   DIAP1: deleted 1-2, N19G, N20V, deleted 146-438; …

   Promoter

   4xUAS

   Available Since

   June 9, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pT22iuasvApoWChe(#488)

    Plasmid

    #184108

    Purpose

    cyclofen-inducible apoptosis activation and imaging (mCherry) in zebrafish permanent transgenic

    Depositor

    Sophie Vriz

    Article

    Feng et al Rep Prog Phys. 2013 Jul;76(7):072601. do

    Insert

    myr-DIAP1-nls'-mCherry-P2A-Casp9-ERT2
    

    Use

    Zebrafish transgenesis (blue eyes)

    Mutation

    DIAP1: deleted 1-2, N19G, N20V, deleted 146-438; …

    Promoter

    4xUAS

    Available Since

    June 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pVRC8400-SARS-CoV-2-RBD-SD1-C.37-AVI

    Plasmid

    #181702

    Purpose

    Expresses SARS-CoV-2 RBD-SD1 domain from the C.37 variant with single chain Fc, HRV3C protease cleavage site, and AVI tag

    Depositor

    Peter Kwong

    Article

    Teng et al PLoS One. 2022 May 24;17(5):e0268767. do

    Insert

    SARS-CoV-2-RBD-SD1-C.37 (S )
    

    Tags

    AVI, HRV3C, and scFc

    Expression

    Mammalian

    Mutation

    L452Q, F490S

    Available Since

    March 2, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pVRC8400-SARS-CoV-2-RBD-SD1-B.1.621-AVI

    Plasmid

    #181703

    Purpose

    Expresses SARS-CoV-2 RBD-SD1 domain from the B.1.621 variant with single chain Fc, HRV3C protease cleavage site, and AVI tag

    Depositor

    Peter Kwong

    Article

    Teng et al PLoS One. 2022 May 24;17(5):e0268767. do

    Insert

    SARS-CoV-2-RBD-SD1-B.1.621 (S )
    

    Tags

    AVI, HRV3C, and scFc

    Expression

    Mammalian

    Mutation

    R346K, E484K, N501Y

    Available Since

    March 2, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pVRC8400-SARS-CoV-2-S2P-B.1.621-AVI

    Plasmid

    #181694

    Purpose

    Expresses SARS-CoV-2 S2P protein from the B.1.621 variant with single chain Fc, HRV3C protease cleavage site, and AVI tag

    Depositor

    Peter Kwong

    Article

    Teng et al PLoS One. 2022 May 24;17(5):e0268767. do

    Insert

    SARS-CoV-2-S2P-B.1.621 (S )
    

    Tags

    AVI, HRV3C, and scFc

    Expression

    Mammalian

    Mutation

    T95I, insert144T, Y144S, Y145N, R346K, E484K, N50…

    Available Since

    March 2, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pVRC8400-SARS-CoV-2-NTD-C.37-AVI

    Plasmid

    #181696

    Purpose

    Expresses SARS-CoV-2 NTD domain from the C.37 variant with single chain Fc, HRV3C protease cleavage site, and AVI tag

    Depositor

    Peter Kwong

    Article

    Teng et al PLoS One. 2022 May 24;17(5):e0268767. do

    Insert

    SARS-CoV-2-NTD-C.37 (S )
    

    Tags

    AVI, HRV3C, and scFc

    Expression

    Mammalian

    Mutation

    G75V, T76I, R246N, ∆S247-∆D253,

    Available Since

    March 2, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pVRC8400-SARS-CoV-2-NTD-B.1.621-AVI

    Plasmid

    #181697

    Purpose

    Expresses SARS-CoV-2 NTD domain from the B.1.621 variant with single chain Fc, HRV3C protease cleavage site, and AVI tag

    Depositor

    Peter Kwong

    Article

    Teng et al PLoS One. 2022 May 24;17(5):e0268767. do

    Insert

    SARS-CoV-2-NTD-B.1.621 (S )
    

    Tags

    AVI, HRV3C, and scFc

    Expression

    Mammalian

    Mutation

    T95I, insert144T, Y144S, Y145N

    Available Since

    March 2, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pVRC8400-SARS-CoV-2-RBD-C.37-AVI

    Plasmid

    #181699

    Purpose

    Expresses SARS-CoV-2 RBD domain from the C.37 variant with single chain Fc, HRV3C protease cleavage site, and AVI tag

    Depositor

    Peter Kwong

    Article

    Teng et al PLoS One. 2022 May 24;17(5):e0268767. do

    Insert

    SARS-CoV-2-RBD-C.37 (S )
    

    Tags

    AVI, HRV3C, and scFc

    Expression

    Mammalian

    Mutation

    L452Q, F490S

    Available Since

    March 2, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pVRC8400-SARS-CoV-2-RBD-B.1.621-AVI

    Plasmid

    #181700

    Purpose

    Expresses SARS-CoV-2 RBD domain from the B.1.621 variant with single chain Fc, HRV3C protease cleavage site, and AVI tag

    Depositor

    Peter Kwong

    Article

    Teng et al PLoS One. 2022 May 24;17(5):e0268767. do

    Insert

    SARS-CoV-2-RBD-B.1.621 (S )
    

    Tags

    AVI, HRV3C, and scFc

    Expression

    Mammalian

    Mutation

    R346K, E484K, N501Y

    Available Since

    March 2, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pVRC8400-SARS-CoV-2-S2P-C.37-AVI

    Plasmid

    #181693

    Purpose

    Expresses SARS-CoV-2 S2P protein from the C.37 variant with single chain Fc, HRV3C protease cleavage site, and AVI tag

    Depositor

    Peter Kwong

    Article

    Teng et al PLoS One. 2022 May 24;17(5):e0268767. do

    Insert

    SARS-CoV-2-S2P-C.37 (S )
    

    Tags

    AVI, HRV3C, and scFc

    Expression

    Mammalian

    Mutation

    G75V, T76I, R246N, ∆S247-∆D253, L452Q, F490S, D61…

    Available Since

    March 2, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pVRC8400-SARS-CoV-2-RBD-SD1-N501Y-A570D-AVI

    Plasmid

    #176449

    Purpose

    Expresses SARS-CoV-2 RBD-SD1 domain containing N501Y-A570D mutations with single chain Fc, HRV3C protease cleavage site, and AVI tag

    Depositor

    Peter Kwong

    Article

    Teng et al PLoS One. 2022 May 24;17(5):e0268767. do

    Insert

    SARS-CoV-2-RBD-SD1-N501Y-A570D (S )
    

    Tags

    AVI, HRV3C, and scFc

    Expression

    Mammalian

    Mutation

    N501Y, A570D

    Available Since

    March 2, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pVRC8400-SARS-CoV-2-RBD-SD1-L452R-AVI

    Plasmid

    #176452

    Purpose

    Expresses SARS-CoV-2 RBD-SD1 domain containing L452R mutation with single chain Fc, HRV3C protease cleavage site, and AVI tag

    Depositor

    Peter Kwong

    Article

    Teng et al PLoS One. 2022 May 24;17(5):e0268767. do

    Insert

    SARS-CoV-2-RBD-SD1-L452R (S )
    

    Tags

    AVI, HRV3C, and scFc

    Expression

    Mammalian

    Mutation

    L452R

    Available Since

    March 2, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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