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pMApra-Ptac

Plasmid

#218357

Purpose

Empty vector with lacIq-Ptac expression system

Depositor

Michael Gebhardt

Publication

Intorcia et al Appl Environ Microbiol. 2024 Sep 18;90(9

Type

Empty backbone

Expression

Bacterial

Available since

May 2, 2024

Availability

Academic Institutions and Nonprofits only

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pBC2103-reci

Plasmid

#117172

Purpose

U6-sgRNA-EFS-arC9-T2A-mCherry-P2A-Hygro; arC9 without NLS

Depositor

David Savage

Publication

Oakes et al Nat Biotechnol. 2016 May 2. doi: 10.1038

Insert

reci_arCas9 without NLS

Use

Lentiviral

Available since

Oct. 12, 2018

Availability

Academic Institutions and Nonprofits only

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pMuc1

Plasmid

#73929

Purpose

Muconate expression plasmid. Expresses enzymes enabling production of muconate from glucose.

Depositor

George Church

Publication

Rogers et al Proc Natl Acad Sci U S A. 2016 Feb 8. pi

Insert

muconate producing enzymes

Available since

June 14, 2017

Availability

Academic Institutions and Nonprofits only

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pAM5087

Plasmid

#85123

Purpose

Ptrc-YFP subcloned from pAM5080 into NSII-Km vector.

Depositor

Susan Golden

Publication

Cohen et al Curr Biol. 2014 Aug 18;24(16):1836-44. d

Insert

YFP

Expression

Bacterial

Available since

June 1, 2017

Availability

Academic Institutions and Nonprofits only

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pDSG319

Plasmid

#115596

Purpose

surface-displayed Ag3, used in composability experiments; medium copy

Depositor

Ingmar Riedel-Kruse

Publication

Glass et al Cell. 2018 Jul 26;174(3):649-658.e16. do

Insert

regulated adhesin construct

Expression

Bacterial

Available since

Oct. 31, 2018

Availability

Academic Institutions and Nonprofits only

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pTE-mcs

Plasmid

#20320

Depositor

Dirk Schnappinger

Publication

Klotzsche et al Nucleic Acids Res. 2009 Apr;37(6):1778-8

Insert

multiple cloning site

Expression

Bacterial

Available since

March 4, 2009

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pAAV_hSyn-PdCO-mScarlet-WPRE (AAV1)

Viral prep

#198510-AAV1

Purpose

Ready-to-use AAV1 particles produced from pAAV_hSyn-PdCO-mScarlet-WPRE (#198510). In addition to the viral particles, you will also receive purified pAAV_hSyn-PdCO-mScarlet-WPRE plasmid DNA. Syn-driven expression of optimized PdCO in frame with mScarlet. These AAV preparations are suitable purity for injection into animals.

Depositor

Ofer Yizhar

Publication

Wietek et al Nat Methods. 2024 May 29. doi: 10.1038/s

Promoter

Syn

Tags

mScarlet

Available since

Jan. 27, 2025

Availability

Academic Institutions and Nonprofits only
MF82_PB-TRE3G-12x42bs-10x(BCRbs_BCRbs)-miniCMV-NLS-FKBP12F36V-BCRZFR39A-VP16-NLS-DHFR-IRES-mCherry-PEST-BGHpA

Plasmid

#176626

Purpose

TF A self-activation cassette in MultiFate-2.2 and MultiFate-3

Depositor

Michael Elowitz

Publication

Zhu et al Science. 2022 Jan 21;375(6578):eabg9765.

Insert

TRE3G-12x42bs-10x(BCRbs_BCRbs)-miniCMV-NLS-FKBP12F36V-BCRZFR39A-VP16-NLS-DHFR-IRES-mCherry-PEST-BGHpA

Use

Synthetic Biology

Available since

Jan. 27, 2022

Availability

Academic Institutions and Nonprofits only

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pSF3-Flag-Cbir-Cdc25C WT_Myc-Nbir-GFP

Plasmid

#90012

Purpose

Split-BioID plasmid: Expresses N-terminally tagged CBir[257-321]-Cdc25C and N-terminally tagged NBir[2-256]-GFP

Depositor

Julien Béthune

Publication

Schopp et al Nat Commun. 2017 Jun 6;8:15690. doi: 10.

Inserts

CBirA*-Cdc25C
NBirA*-GFP

Use

Retroviral; Flp/frt

Tags

FLAG and Myc

Expression

Mammalian

Promoter

CMV

Available since

Aug. 23, 2017

Availability

Academic Institutions and Nonprofits only

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pAAV-CMV-DIO-TRPV4-p2A-ferritin-sNRPpA

Plasmid

#74306

Purpose

Bicistronic Cre-dependent expression of TRPV4 & ferritin in mammalian cells

Depositor

Ali Guler

Publication

Wheeler et al Nat Neurosci. 2016 Mar 7. doi: 10.1038/n

Inserts

TRPV4 (Trpv4 Rat)
ferritin (FTL and HTL1) (FTL, FTH1 Human)

Use

AAV and Cre/Lox

Tags

FLAG and p2A

Expression

Mammalian

Promoter

CMV (after cre recombination)

Available since

July 18, 2016

Availability

Academic Institutions and Nonprofits only

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ePE2

Plasmid

#202029

Purpose

Prime Editing plasmid for tag insertion

Depositor

Pengcheng Wei

Publication

Li et al Plant Commun. 2023 Mar 7:100572. doi: 10

Insert

Cas9，

Use

CRISPR

Expression

Plant

Available since

Aug. 24, 2023

Availability

Academic Institutions and Nonprofits only

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pAGM37443

Plasmid

#153218

Purpose

Level 2 binary vector for plant transformation; for cloning Cas9 and guide RNAs; single copy in Agrobacterium.

Depositor

Sylvestre Marillonnet

Publication

Grutzner et al Plant Commun. 2020 Nov 23;2(2):100135. d

Type

Empty backbone

Expression

Plant

Available since

Aug. 18, 2020

Availability

Academic Institutions and Nonprofits only

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pHlyIIR-H1

Plasmid

#74681

Purpose

HlyIIR-H1 gate

Depositor

Christopher Voigt

Publication

Nielsen et al Science. 2016 Apr 1;352(6281):aac7341. d

Insert

PTac-RBSH1-HlyIIR-PHlyIIR-YFP

Expression

Bacterial

Available since

May 18, 2016

Availability

Academic Institutions and Nonprofits only

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pAmtR-A1

Plasmid

#74676

Purpose

AmtR-A1 gate

Depositor

Christopher Voigt

Publication

Nielsen et al Science. 2016 Apr 1;352(6281):aac7341. d

Insert

PTac-RBSA1-AmtR-PAmtR-YFP

Expression

Bacterial

Available since

May 9, 2016

Availability

Academic Institutions and Nonprofits only

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TOPO mCherry

Plasmid

#68490

Purpose

TOPO construct expressing mCherry for generation of GMAP-compatible gene

Depositor

Tyler Jacks

Publication

Akama-Garren et al Sci Rep. 2016 Feb 18;6:16836. doi: 10.10

Insert

mCherry

Use

Gmap

Expression

Bacterial

Promoter

Plac

Available since

Feb. 22, 2016

Availability

Academic Institutions and Nonprofits only

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pTK317

Plasmid

#59571

Purpose

TC-83 I-SceI T7 5'UTR (A3G) P1234 (nsP2 Q739L) SGP(16) 2xK-turn Kozak EGFP attB2 AscI (truncated E1) 3'UTR Poly A I-SceI

Depositor

Ron Weiss

Publication

Wroblewska et al Nat Biotechnol. 2015 Aug 3. doi: 10.1038

Insert

SGP(16) 2xK-turn Kozak EGFP attB2 AscI

Available since

Aug. 3, 2015

Availability

Academic Institutions and Nonprofits only

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TS_pLuxLac

Plasmid

#140426

Purpose

Toggle switch circuit responsive to AHL gradient.

Depositor

Yolanda Schaerli

Publication

Barbier et al Mol Syst Biol. 2020 Jun;16(6):e9361. doi

Inserts

GFP
LacI
TetR
mCherry

Use

Synthetic Biology

Expression

Bacterial

Available since

June 15, 2020

Availability

Academic Institutions and Nonprofits only

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pZS2oxySp*-RBS30-Bxbi-proD-oxyR

Plasmid

#78211

Purpose

Synthetic gene circuit

Depositor

Timothy Lu

Publication

Rubens et al Nat Commun. 2016 Jun 3;7:11658. doi: 10.

Insert

synthetic circuit

Available since

May 3, 2017

Availability

Academic Institutions and Nonprofits only

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pC236

Plasmid

#65951

Purpose

Medium Repressor plasmid for two-strain system

Depositor

Matthew Bennett

Publication

Chen et al Science. 2015 Aug 28;349(6251):986-9. do

Insert

CinI

Tags

LAA tagged

Expression

Bacterial

Promoter

Prhl/lac-m

Available since

Aug. 13, 2015

Availability

Academic Institutions and Nonprofits only

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pYTK081

Plasmid

#65188

Purpose

Encodes CEN6/ARS4 as a Type 7 part to be used in the Dueber YTK system

Depositor

John Dueber

Publication

Lee et al ACS Synth Biol. 2015 Apr 14.

Insert

CEN6/ARS4

Expression

Bacterial

Available since

June 16, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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