We narrowed to 17,649 results for: BLI

1. pBK-Ma pylRS nitroY/haloY-F5

   Plasmid

   #212125

   Purpose

   Expresses Methanomethylophilus alvus pyrrolysine tRNA synthetase engineered for 3-nitroY and 3-haloY under GlnS promoter. Used as a control for Ma Pyl tRNA-synthetase selections.

   Depositor

   Ryan Mehl

   Insert

   M. alvus PylRS 3-NY/HaloY F5
   

   Expression

   Bacterial

   Mutation

   CTG125CTT, N166A V168S, A223C, W239R

   Promoter

   GlnS (constitutive)

   Available Since

   Aug. 28, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. AICSDP-117:POLR2A-mEGFP

   Plasmid

   #164500

   Purpose

   Homology arms and mEGFP-linker sequence sequence for N-terminus tagging of human POLR2A

   Depositor

   Allen Institute for Cell Science

   Insert

   POLR2A Homology Arms with mEGFP-linker (POLR2A Human)
   

   Use

   CRISPR; Donor template

   Tags

   mEGFP-linker

   Expression

   Mammalian

   Mutation

   homology arms contain point mutations to disrupt …

   Available Since

   Feb. 17, 2021

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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3. psd44-G12QL

   Plasmid

   #46825

   Purpose

   expression of mutant G protein alpha 12 in mammalian cells

   Depositor

   Agnese Mariotti

   Insert

   GNA12 (GNA12 Human)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Mutation

   Q231L (the mutation reduces GTPase activity resul…

   Promoter

   PGK

   Available Since

   July 30, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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4. pAG416-GAL-FUS-G156E

   Plasmid

   #181716

   Purpose

   Galactose inducible expression of FUS-G156E

   Depositor

   Alejandro Chavez

   Insert

   FUS (FUS Human)
   

   Expression

   Yeast

   Mutation

   G156E

   Available Since

   April 24, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. Hs.KRAS4a

   Plasmid

   #83166

   Purpose

   Gateway ORF Entry clone of human KRAS4A [NM_033360.3] with stop codon (for native or N-terminal fusions)

   Depositor

   Dominic Esposito

   Insert

   KRAS (KRAS Human)
   

   Use

   Gateway entry clone

   Available Since

   Sept. 14, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. pAAV-CaMKIIa-HA-hM4D(Gi)-IRES-mCitrine

   Plasmid

   #50467

   Purpose

   Gi-coupled hM4D-IRES-mCitrine under the control of CaMKIIa promoter

   Depositor

   Bryan Roth

   Has Service

   AAV2 and AAV8

   Insert

   hM4D(Gi)-IRES-mCitrine (CHRM4 Human)
   

   Use

   AAV

   Tags

   HA

   Mutation

   See supplemental documents for DREADD mutations

   Promoter

   CaMKIIa

   Available Since

   April 16, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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7. pAAV-GFAP-HA-hM4D(Gi)-IRES-mCitrine

   Plasmid

   #50471

   Purpose

   Gi-coupled hM4D-IRES-mCitrine under the control of GFAP promoter

   Depositor

   Bryan Roth

   Insert

   hM4D(Gi)-IRES-mCitrine (CHRM4 Human)
   

   Use

   AAV

   Tags

   HA

   Mutation

   See supplemental documents for DREADD mutations

   Promoter

   GFAP

   Available Since

   April 16, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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8. 6xO/S luc

   Plasmid

   #69445

   Purpose

   detection/assessment of OCT4 and SOX2 transcription factor activity

   Depositor

   Lisa Dailey

   Insert

   minimal fgf4 promoter and 6XOS tandem repeat of composite OCT4/Sox2 binding site from FGF4 enhancer
   

   Use

   Luciferase

   Expression

   Mammalian

   Available Since

   May 5, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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9. Hs.KRAS4b G13D

   Plasmid

   #83133

   Purpose

   Gateway ORF Entry clone of human KRAS4B [NM_004985.4 ] with stop codon (for native or N-terminal fusions), G13D mutation

   Depositor

   Dominic Esposito

   Insert

   KRAS (KRAS Human)
   

   Use

   Gateway entry clone

   Mutation

   G13D

   Available Since

   Sept. 13, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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10. ACACA

    Plasmid

    #39083

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Nicola Burgess-Brown

    Insert

    ACACA (ACACA Human)
    

    Tags

    His Tev

    Expression

    Bacterial

    Mutation

    codon-optimized

    Available Since

    March 18, 2013

    Availability

    Academic Institutions and Nonprofits only

11. SARS-CoV-2 Spike-d18

    Plasmid

    #164583

    Purpose

    A mammalian expression vector to express codon-optimized SARS-CoV-2 Spike protein, with a C terminal truncation of 18 amino acids to improve pseudoparticle generation.

    Depositor

    Jason Sheltzer

    Insert

    SARS-CoV-2 Spike (S SARS-CoV-2)
    

    Expression

    Mammalian

    Mutation

    Deleted amino acids 1256-1273

    Promoter

    EF1a

    Available Since

    Jan. 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. Lamin A (G465D)-mRFP

    Plasmid

    #124271

    Purpose

    Lamin A mutant (G465D), C-terminally tagged with mRFP. Mutation associated with FPLD lipodystrophy.

    Depositor

    Eric Schirmer

    Insert

    LMNA (G465D) (LMNA Human)
    

    Tags

    mRFP

    Expression

    Mammalian

    Mutation

    LMNA (G465D)

    Available Since

    May 10, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. EEF1A2 in pENTR

    Plasmid

    #216585

    Purpose

    gateway cloning donor vector containing EEF1A2

    Depositor

    Douglas Allan, Christopher Loewen

    Insert

    EEF1A2 (EEF1A2 Human)
    

    Use

    Gateway entry plasmid

    Available Since

    March 27, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. pOET5 CVB3-VLP

    Plasmid

    #218146

    Purpose

    Forms coxsackievirus 3-like particle in insect cells by expression of VP1–VP4 polyprotein (GeneID M33854.1) and 3CD protease (GeneID M33854.1).

    Depositor

    Minna Hankaniemi

    Inserts

    VP1–VP4
    3CD
    

    Expression

    Insect

    Promoter

    CMV and Polyhedrin

    Available Since

    May 13, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. pLPS-hSox2-gfp

    Plasmid

    #49390

    Purpose

    mammalian expression of human Sox2 fused to EGFP

    Depositor

    Randall McKinnon

    Insert

    Sox2 (SOX2 Human)
    

    Use

    Cre/Lox; Acceptor vector

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    TGA (stop) to CCA (Pro) at nt 1027 with respect t…

    Promoter

    CMV

    Available Since

    Nov. 21, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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16. pGWB2-CYCD3;1

    Plasmid

    #140750

    Purpose

    Inducing plant cells to enter mitosis

    Depositor

    Bo Liu

    Article

    Xu et al New Phytol. 2019 Nov 2. doi: 10.1111/nph

    Insert

    Cyclin D3;1 (CYCD3;1 Mustard Weed)
    

    Expression

    Plant

    Promoter

    35S

    Available Since

    May 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. Hs.KRAS4b G12C

    Plasmid

    #83130

    Purpose

    Gateway ORF Entry clone of human KRAS4B [NM_004985.4 ] with stop codon (for native or N-terminal fusions), G12C mutation

    Depositor

    Dominic Esposito

    Insert

    KRAS (KRAS Human)
    

    Use

    Gateway entry clone

    Mutation

    G12C

    Available Since

    Sept. 13, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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18. AICSDP-69: NPM1-mTagRFP-T

    Plasmid

    #124608

    Purpose

    Homology arms and linker-mTagRFP-T sequence for C-terminus tagging of human NPM1

    Depositor

    Allen Institute for Cell Science

    Insert

    NPM1 Homology Arms with linker-mTagRFP-T (NPM1 Human)
    

    Use

    CRISPR; Donor template

    Expression

    Mammalian

    Available Since

    May 13, 2019

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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19. Hs.KRAS4a G12D

    Plasmid

    #83168

    Purpose

    Gateway ORF Entry clone of human KRAS4A [NM_033360.3] with stop codon (for native or N-terminal fusions), G12D mutation

    Depositor

    Dominic Esposito

    Insert

    KRAS (KRAS Human)
    

    Use

    Gateway entry clone

    Mutation

    G12D

    Available Since

    Sept. 14, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCAGGS-N2c(G)

    Plasmid

    #73481

    Purpose

    N2c Glycoprotein expression vector

    Depositor

    Thomas Jessell

    Article

    Reardon et al Neuron. 2016 Jan 19. pii: S0896-6273(16)

    Insert

    N2c(G)
    

    Expression

    Mammalian

    Available Since

    March 21, 2016

    Availability

    Academic Institutions and Nonprofits only