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pCF525-mTagBFP2_EF1a-Hygro-P2A-mTagBFP2_lenti

Plasmid

#138315

Purpose

Lenti expression of mTgBFP2

Depositor

Jennifer Doudna

Publication

Watters et al Proc Natl Acad Sci U S A. 2020 Mar 10. p

Insert

mTAGBFP2

Use

CRISPR and Lentiviral

Expression

Mammalian

Available since

March 17, 2020

Availability

Academic Institutions and Nonprofits only

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pMx-puro-MGT

Plasmid

#111809

Purpose

Retroviral expression vector for direct cardiac reprogramming

Depositor

Li Qian

Publication

Wang et al Circ Res. 2015 Jan 16;116(2):237-44. doi

Insert

Mef2c, Tbx5 and Gata4 (Gata4, Mef2c, Tbx5 Mouse)

Use

Retroviral

Expression

Mammalian

Promoter

viral LTR

Available since

Aug. 20, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

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pGL4.18 CMV-Luc

Plasmid

#100984

Purpose

CMV luciferase reporter - firefly luciferase driven by CMV promoter

Depositor

Lee Helman

Publication

Grohar et al J Natl Cancer Inst. 2011 Jun 22;103(12):

Insert

CMV

Expression

Mammalian

Available since

May 1, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pTO_CHMP4B_LAP

Plasmid

#101850

Purpose

Tet-inducible expression of human CHMP4B with a C-terminal GFP and long linker (LAP tag), compatible with Flp-In recombination for stable integration

Depositor

Daniel Gerlich

Publication

Mierzwa et al Nat Cell Biol. 2017 Jul;19(7):787-798. d

Insert

Charged Multivesicular Body Protein 4B (CHMP4B Human)

Use

Gateway cloning, flp-frt recombination, tet-induc…

Tags

Localization and Affinity Purification (LAP) tag

Expression

Bacterial and Mammalian

Promoter

CMV

Available since

Dec. 12, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pMXs-mGCN2-FLAG-IP

Plasmid

#101794

Purpose

Retroviral vector encoding C-terminally FLAG tagged mouse GCN2

Depositor

Seiichi Oyadomari

Publication

Taniuchi et al Sci Rep. 2016 Sep 16;6:32886. doi: 10.10

Insert

Gcn2 (Eif2ak4 Mouse)

Use

Retroviral

Tags

3xFLAG

Expression

Mammalian

Promoter

LTR

Available since

Oct. 17, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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mApple-Sec61b-C1

Plasmid

#90993

Purpose

Expresses mApple-tagged Sec61b, brightly labels endoplasmic reticulum in mammalian cells

Depositor

Jennifer Lippincott-Schwartz

Publication

Nixon-Abell et al Science. 2016 Oct 28;354(6311). pii: aaf

Insert

mApple-Sec61b (SEC61B Synthetic, Human)

Tags

mApple (red fluorescence)

Expression

Mammalian

Promoter

CMV

Available since

June 12, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pDONR223_PIK3CA_WT

Plasmid

#81736

Purpose

Gateway Donor vector containing PIK3CA , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Publication

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

PIK3CA (PIK3CA Human)

Use

Gateway Cloning

Mutation

WT

Promoter

None

Available since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pGWB535

Plasmid

#74873

Purpose

Gateway cloning compatible binary vector for C-terminal fusion with LUC (no promoter).

Depositor

Tsuyoshi Nakagawa

Publication

Nakagawa et al Biosci Biotechnol Biochem. 2007 Aug;71(8

Type

Empty backbone

Expression

Plant

Promoter

No Promoter

Available since

Oct. 14, 2016

Availability

Academic Institutions and Nonprofits only

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pGWB433

Plasmid

#74824

Purpose

Gateway cloning compatible binary vector for C-terminal fusion with GUS (no promoter).

Depositor

Tsuyoshi Nakagawa

Publication

Nakagawa et al Biosci Biotechnol Biochem. 2007 Aug;71(8

Type

Empty backbone

Expression

Plant

Available since

July 14, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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GFP-mouse vinculin full length (889)

Plasmid

#67935

Purpose

Mammalian expression of mouse vinculin with GFP fusion

Depositor

Alpha Yap

Publication

Leerberg et al Curr Biol. 2014 Aug 4;24(15):1689-99. do

Insert

Vinculin (Vcl Mouse)

Tags

EGFP

Expression

Mammalian

Mutation

L159P in vinculin

Promoter

CMV

Available since

Nov. 18, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

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GIV-WT - FLAG

Plasmid

#65947

Purpose

Expression GIV/Girdin in Mammalian cell

Depositor

Pradipta Ghosh

Publication

Lin et al Mol Biol Cell. 2014 Nov 5;25(22):3654-71

Insert

GIV/Girdin (CCDC88A Human)

Tags

3xFlag

Expression

Mammalian

Promoter

CMV

Available since

Oct. 15, 2015

Availability

Academic Institutions and Nonprofits only
pCDEF3‐hTIM3 (no tag)

Plasmid

#49212

Purpose

expresses human Tim3

Depositor

Lawrence Kane

Publication

Lee et al Mol Cell Biol. 2011 Oct;31(19):3963-74.

Insert

Tim3 (HAVCR2 Human)

Expression

Mammalian

Promoter

EF1a

Available since

Dec. 10, 2013

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pEYFP-Plk1(499)

Plasmid

#39843

Depositor

Jonathon Pines

Publication

Lindon et al J Cell Biol. 2004 Jan 19;164(2):233-41.

Insert

Plk1 (PLK1 Human)

Tags

EYFP

Expression

Mammalian

Available since

Oct. 31, 2012

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pEYFP-Plk1(499)

Plasmid

#39843

Depositor

Jonathon Pines

Publication

Lindon et al J Cell Biol. 2004 Jan 19;164(2):233-41.

Insert

Plk1 (PLK1 Human)

Tags

EYFP

Expression

Mammalian

Available since

Oct. 31, 2012

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pJA304

Plasmid

#28091

Depositor

Julie Ahringer

Publication

Zeiser et al PLoS One. 2011;6(5):e20082. Epub 2011 Ma

Insert

(Gly)5Ala::mCherry(with introns)::tbb-2 3'UTR

Expression

Worm

Available since

April 7, 2011

Availability

Academic Institutions and Nonprofits only

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TRE-TIGHT-EGFP-backward donor

Plasmid

#22077

Depositor

Rudolf Jaenisch

Publication

Hockemeyer et al Nat Biotechnol. 2009 Sep;27(9):851-7. do

Insert

TETO-eGFP

Available since

Oct. 5, 2009

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

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TRE-TIGHT-EGFP-backward donor

Plasmid

#22077

Depositor

Rudolf Jaenisch

Publication

Hockemeyer et al Nat Biotechnol. 2009 Sep;27(9):851-7. do

Insert

TETO-eGFP

Available since

Oct. 5, 2009

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

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pEF-ENTR-LacZ (710-1)

Plasmid

#17430

Purpose

Gateway entry vector with EF1-a promoter, V5-His C-term, Lac Z

Depositor

Eric Campeau, Paul Kaufman

Publication

Campeau et al PLoS One. 2009 Aug 6;4(8):e6529.

Insert

LacZ

Use

Gateway Cloning

Tags

His and V5

Expression

Mammalian

Available since

Aug. 12, 2009

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pEF-ENTR-LacZ (710-1)

Plasmid

#17430

Purpose

Gateway entry vector with EF1-a promoter, V5-His C-term, Lac Z

Depositor

Eric Campeau, Paul Kaufman

Publication

Campeau et al PLoS One. 2009 Aug 6;4(8):e6529.

Insert

LacZ

Use

Gateway Cloning

Tags

His and V5

Expression

Mammalian

Available since

Aug. 12, 2009

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pMSCV PIG IMP-1-short

Plasmid

#21659

Depositor

David Bartel

Publication

Mayr et al Cell. 2009 Aug 21. 138(4):673-84.

Insert

IGF-II mRNA-binding protein 1 (IGF2BP1 Human)

Use

Retroviral

Tags

GFP and IRES

Expression

Mammalian

Available since

July 22, 2009

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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