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SARS_COV2_ORF7a_HA_BASU

Plasmid

#153983

Purpose

Express Orf7a gene along with HA and BASU

Depositor

Paul Khavari

Article

Meyers et al PLoS Pathog. 2021 Oct 1;17(10):e1009412.

Insert

orf7a (ORF7a Severe acute respiratory syndrome coronavirus 2)

Use

Lentiviral

Expression

Mammalian

Available Since

Oct. 5, 2021

Availability

Academic Institutions and Nonprofits only

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pDEST27-GST-ATXN1 FL(84Q)

Plasmid

#21754

Depositor

Huda Zoghbi

Article

Lim et al Nature. 2008 Apr 10. 452(7188):713-8.

Insert

Ataxin-1 (ATXN1 Human)

Tags

GST

Expression

Mammalian

Mutation

insert contains a stretch of 84 Glutamines and no…

Promoter

CMV

Available Since

July 22, 2014

Availability

Academic Institutions and Nonprofits only
pcDNA 3.1-nsp7-FLAG

Plasmid

#201027

Purpose

Expresses C-terminally FLAG tagged SARS-CoV-2 nsp7 in mammalian.

Depositor

Sharon Rozovsky

Article

Ghelichkhani et al J Mol Biol. 2023 Apr 15;435(8):168008. d

Insert

non-structural protein 7 (ORF1ab Synthetic, Severe acute respiratory syndrome coronavirus 2)

Tags

8xHis and FLAG

Expression

Mammalian

Promoter

T7 promoter

Available Since

May 26, 2023

Availability

Academic Institutions and Nonprofits only

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pEE065

Plasmid

#176822

Purpose

Encodes codon optimized putative thermophilic PET hydrolase enzyme for bacterial expression

Depositor

Gregg Beckham

Article

Erickson et al Nat Commun. 2022 Dec 21;13(1):7850. doi:

Insert

704

Tags

6xHis

Expression

Bacterial

Promoter

T7

Available Since

Nov. 28, 2022

Availability

Academic Institutions and Nonprofits only

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pRQ-hRasV12

Plasmid

#18113

Depositor

Alex Swarbrick

Article

Swarbrick et al Proc Natl Acad Sci U S A. 2008 Apr 8. 10

Insert

h-RasV12G (HRAS Human)

Use

Retroviral

Expression

Mammalian

Mutation

G12V, constitutively active

Available Since

Feb. 9, 2009

Availability

Academic Institutions and Nonprofits only

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pmirGLO-3'UTR mouse Il13

Plasmid

#207125

Purpose

Luciferase vector containing 3'UTR for mouse Il13

Depositor

Silvia Monticelli

Article

Leoni et al Nat Commun. 2023 Jun 29;14(1):3862. doi:

Insert

Interleukin 13 (Il13) 3'UTR (Il13 Mouse)

Use

Luciferase

Expression

Mammalian

Promoter

PGK promoter

Available Since

Dec. 5, 2023

Availability

Academic Institutions and Nonprofits only

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HA-PRAS40 AA

Plasmid

#86759

Purpose

N-terminal HA-tagged protein expression in mammalian cells

Depositor

Do-Hyung Kim

Article

Yun et al Mol Cell. 2016 Feb 18;61(4):625-39. doi:

Insert

human PRAS40 (AKT1S1 Human)

Tags

HA

Expression

Mammalian

Mutation

AA (S183A, S221A)

Promoter

CMV

Available Since

Jan. 26, 2017

Availability

Academic Institutions and Nonprofits only

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pCAG-hMb3Cas12a-NLS(nucleoplasmin)-3xHA (RTW2500)

Plasmid

#115142

Purpose

CAG promoter expression plasmid for human codon optimized Mb3Cas12a nuclease with C-terminal NLS and HA tag

Depositor

Joseph Bondy-Denomy, Keith Joung, Benjamin Kleinstiver

Article

Marino et al Science. 2018 Sep 6. pii: science.aau517

Insert

human codon optimized Mb3Cas12a

Tags

NLS(nucleoplasmin) and 3xHA

Expression

Mammalian

Available Since

Sept. 11, 2018

Availability

Academic Institutions and Nonprofits only

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pCMV-BEACON1

Plasmid

#171697

Purpose

Expresses BEACON1 in mammalian cells

Depositor

Jia Chen

Article

Wang et al Cell Rep. 2020 Jun 2;31(9):107723. doi:

Insert

BEACON1

Use

CRISPR

Expression

Mammalian

Mutation

hAPOBEC3A_W104A/Y132D

Promoter

CMV

Available Since

Aug. 9, 2021

Availability

Academic Institutions and Nonprofits only

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MERS-coV-NSP3-c001

Plasmid

#173085

Purpose

Expression of recombinant fusion protein in E. coli

Depositor

Lari Lehtiö

Article

Sowa et al Cell Rep Methods. 2021 Dec 20;1(8):10012

Insert

rep (orf1ab Middle East respiratory syndrome-related coronavirus (isolate United Kingdom/H123990006/2012) (MERS-CoV))

Tags

mCerulean

Expression

Bacterial

Mutation

D1109-D1275, codon optimized

Available Since

Dec. 9, 2021

Availability

Academic Institutions and Nonprofits only

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SHIP2 WT pBABE-His-Xpress

Plasmid

#126584

Purpose

Retroviral infection of MEF cells (puromycin selection)

Depositor

Christophe Erneux

Article

Zhang et al Cell Signal. 2007 Oct;19(10):2194-200. d

Insert

SHIP2 (INPPL1 Human)

Use

Retroviral

Tags

His Xpress (N-terminal)

Available Since

July 2, 2019

Availability

Academic Institutions and Nonprofits only

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pTRIP-SFFV-GFP-RELA K310R

Plasmid

#196630

Purpose

Express GFP-RELA K310R

Depositor

Nicolas Manel

Article

Jeremiah et al J Exp Med. 2023 May 1;220(5):e20220666.

Insert

GFP-RELA K310R (RELA Human)

Use

Lentiviral

Tags

GFP

Mutation

K310R

Available Since

Feb. 24, 2023

Availability

Academic Institutions and Nonprofits only

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pCDNAIIIB rac1 QL

Plasmid

#61633

Purpose

mammalian expression of rac1 constitutively active mutant

Depositor

JS Gutkind

Article

Coso et al Cell. 1995 Jun 30;81(7):1137-46.

Insert

Rac1 (RAC1 Human)

Expression

Mammalian

Mutation

Q61L, constitutively active

Promoter

CMV

Available Since

Jan. 26, 2015

Availability

Academic Institutions and Nonprofits only
pcDNA5FRT/TO-PLAA-Strep-HA

Plasmid

#113484

Purpose

Expression of human PLAA with C-terminal strep-HA tag

Depositor

Hemmo Meyer

Article

Hulsmann et al Mol Cell Proteomics. 2018 Mar 29. pii: R

Insert

PLAA (PLAA Human)

Tags

2xstrep-HA

Expression

Mammalian

Mutation

wildtype without stop codon

Promoter

CMV

Available Since

Aug. 14, 2018

Availability

Academic Institutions and Nonprofits only

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GFP-IP3KC

Plasmid

#134603

Purpose

Expresses IP3KC GFP tagged

Depositor

Christophe Erneux

Article

Dewaste et al Biochem J. 2003 Aug 15;374(Pt 1):41-9.

Insert

IP3KC (ITPKC Human)

Tags

GFP

Expression

Mammalian

Available Since

Nov. 22, 2019

Availability

Academic Institutions and Nonprofits only

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pAAV-EFIa-DIO-LMO7 (mNeonGreen-eKL9h-VChR1)

Plasmid

#205098

Purpose

Cre-dependent fusion protein of mNeonGreen, eKL9h, and Volvox channelrhodopsin-1 (luminopsin LMO7) for bioluminescent optogenetics

Depositor

Ute Hochgeschwender

Article

Björefeldt et al Neurophotonics. 2024 Apr;11(2):021005. d

Insert

mNeonGreen, eKL9h, and Volvox channelrhodopsin-1

Use

AAV

Promoter

hSyn

Available Since

Sept. 15, 2023

Availability

Academic Institutions and Nonprofits only

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pT2ADW-lox-mCherryNLS-pA-lox-4493NES

Plasmid

#138374

Purpose

Booster-PKA for the generation of mice

Depositor

Michiyuki Matsuda

Article

Watabe et al ACS Sens. 2020 Feb 26. doi: 10.1021/acss

Insert

lox-mCherry-lox-Booster-PKA

Expression

Mammalian

Promoter

CAG

Available Since

March 4, 2020

Availability

Academic Institutions and Nonprofits only

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pFRT_TO_FlagHA_CELF1

Plasmid

#106099

Purpose

Inducible gene expression of FlagHA - CELF1

Depositor

Thomas Tuschl

Article

Meyer et al Mol Cell. 2018 Feb 15;69(4):622-635.e6.

Insert

CELF1 (CELF1 Human)

Tags

Flag and HA

Expression

Mammalian

Available Since

Feb. 12, 2018

Availability

Academic Institutions and Nonprofits only

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pDEST27-GST-ATXN1 FL(30Q)

Plasmid

#21753

Depositor

Huda Zoghbi

Article

Lim et al Nature. 2008 Apr 10. 452(7188):713-8.

Insert

Ataxin-1 (ATXN1 Human)

Tags

GST

Expression

Mammalian

Mutation

insert contains a stretch of 32 amino acids (30 Q…

Promoter

CMV

Available Since

July 18, 2014

Availability

Academic Institutions and Nonprofits only
pEE062

Plasmid

#176819

Purpose

Encodes codon optimized putative thermophilic PET hydrolase enzyme for bacterial expression

Depositor

Gregg Beckham

Article

Erickson et al Nat Commun. 2022 Dec 21;13(1):7850. doi:

Insert

701

Tags

6xHis

Expression

Bacterial

Promoter

T7

Available Since

Nov. 28, 2022

Availability

Academic Institutions and Nonprofits only

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