We narrowed to 42,093 results for: Eras

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EV-A71 2A protease; 6xHis-MBP-TEV tag, self cleaving

Plasmid

#204811

Purpose

Bacterial expresison of Enterovirus A71 2A protease

Depositor

ASAP Discovery, Frank von Delft

Insert

A71 2A protease

Expression

Bacterial

Available Since

Nov. 16, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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AgAK

Plasmid

#71290

Purpose

Expression of A. gambiae adenosine kinase

Depositor

Vern Schramm

Article

Cassera et al Biochemistry. 2011 Mar 22;50(11):1885-93

Insert

Adenosine Kinase

Tags

6X Histidine

Expression

Bacterial

Promoter

T7

Available Since

Nov. 19, 2015

Availability

Academic Institutions and Nonprofits only
pSplit-NO-Dib1

Plasmid

#58786

Purpose

Express LucN-hDib1 fusion protein in mammalian cells

Depositor

Hiroyoshi Ariga

Article

Maita et al Anal Biochem. 2014 Feb 3. pii: S0003-269

Insert

hDIb1

Tags

Flag and Luciferase (1-398aa)

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 15, 2014

Availability

Academic Institutions and Nonprofits only
pFLuc190UGA

Plasmid

#41046

Depositor

James Inglese

Article

Auld et al Proc Natl Acad Sci U S A. 2009 Mar 3;106

Insert

Firefly luciferase 190UGA

Expression

Mammalian

Mutation

in-frame UGA stop codon was created at codon 190

Promoter

CMV

Available Since

Nov. 9, 2012

Availability

Academic Institutions and Nonprofits only
pUC57-Rluc-A114-N40

Plasmid

#50561

Purpose

Template DNA for in vitro transcription using T7 polymerase

Depositor

Yukihide Tomari

Article

Fukaya et al EMBO J. 2011 Nov 25;30(24):4998-5009. do

Insert

Luciferase-A114-N40

Use

Cloning vector

Promoter

lac

Available Since

Feb. 10, 2014

Availability

Academic Institutions and Nonprofits only

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iE61 PB-Zim3-XTEN-dCas9-mScarlet-puro-BFP

Plasmid

#204722

Purpose

Constitutive CRISPRi by Zim3-dCas9

Depositor

iPSC Neurodegenerative Disease Initiative (iNDI), Michael Ward

Insert

Zim3-dCas9

Expression

Mammalian

Available Since

Aug. 7, 2023

Availability

Academic Institutions and Nonprofits only

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NLS-LucDM-GFP

Plasmid

#127796

Purpose

The expressed ORF encodes nucleus location signal with GFP and destabilized mutant Luciferase.

Depositor

Xiaolu Yang

Article

Guo et al J Vis Exp. 2016 Aug 28;(114). doi: 10.37

Insert

destabilized mutant (DM) Luciferase

Tags

EGFP and FLAG-NLS

Expression

Mammalian

Mutation

R188Q, R261Q in Firefly Luciferase insert

Promoter

CMV

Available Since

July 5, 2019

Availability

Academic Institutions and Nonprofits only

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pUC119 T7 hTRmin

Plasmid

#127858

Purpose

T7 RNA Pol for in vitro transcription

Depositor

Kathleen Collins

Article

Wu et al EMBO J. 2014 Apr 16;33(8):921-35. doi: 1

Insert

hTR (TERC Human)

Available Since

Aug. 13, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA3 xNoggin

Plasmid

#24960

Depositor

Edward De Robertis

Article

Pera et al Int J Dev Biol. 2005 . 49(7):781-96.

Insert

Noggin (nog.L Frog)

Expression

Mammalian

Available Since

June 18, 2010

Availability

Academic Institutions and Nonprofits only

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pLKO.1C-shSCR

Plasmid

#139472

Purpose

Replacing the RSV promoter of pLKO.1 with the CMV promoter improves lentiviral production with third-generation viral packaging system.

Depositor

David Cobrinik

Article

Lee et al Biotechniques. 2020 Jun;68(6):349-352. d

Insert

shSCR

Use

Lentiviral and RNAi

Expression

Mammalian

Promoter

CMV

Available Since

July 14, 2021

Availability

Academic Institutions and Nonprofits only

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VCP_Halo_C_allele

Plasmid

#178132

Purpose

Donor vector for endogenous tagging of human VCP at the C-terminus with halotag

Depositor

iPSC Neurodegenerative Disease Initiative (iNDI), Michael Ward

Insert

Halotag (VCP Human)

Use

Donor vector

Available Since

Dec. 2, 2021

Availability

Academic Institutions and Nonprofits only

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NK644

Plasmid

#109096

Purpose

Reporter gene expression in S. rosetta

Depositor

Nicole King

Article

Booth et al Mol Biol Cell. 2018 Dec 1;29(25):3026-30

Insert

PTSG_00306 geranyl-geranylation signal

Use

Choanoflagellate expression

Available Since

May 15, 2018

Availability

Academic Institutions and Nonprofits only

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MSCV-SNAP-Smo

Plasmid

#31619

Depositor

Rajat Rohatgi

Article

Milenkovic et al J Cell Biol. 2009 Nov 2. 187(3):365-74.

Insert

Smo (Smo Mouse)

Use

Retroviral

Tags

SNAP

Expression

Mammalian

Available Since

Aug. 18, 2011

Availability

Academic Institutions and Nonprofits only
pGL3-p107 WT

Plasmid

#32392

Depositor

Julien Sage

Article

Burkhart et al Mol Cell Biol. 2010 Apr;30(7):1729-45. E

Insert

p107 promoter (Rbl1 Mouse)

Use

Luciferase

Tags

Luciferase

Expression

Mammalian

Available Since

Sept. 27, 2011

Availability

Academic Institutions and Nonprofits only
iD20 LoxStopLox-Zim3-dCas9-mApple-hygro

Plasmid

#204720

Purpose

Cre-inducible CRISPRi by Zim3-dCas9

Depositor

iPSC Neurodegenerative Disease Initiative (iNDI), Michael Ward

Insert

Zim3-dCas9

Expression

Mammalian

Available Since

Aug. 4, 2023

Availability

Academic Institutions and Nonprofits only

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PP93

Plasmid

#78990

Purpose

Supplemental Figure 4. No Promoter.

Depositor

Timothy Lu

Article

Perez-Pinera et al Nat Commun. 2016 Jul 29;7:12211. doi: 10

Insert

GFP

Expression

Yeast

Available Since

Aug. 23, 2016

Availability

Academic Institutions and Nonprofits only

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pET15b-At 14-3-3 omega

Plasmid

#44930

Depositor

Robert Ferl

Article

Wu et al Arch Biochem Biophys. 1997 Mar 1;339(1):

Insert

14-3-3 omega (GRF2 Mustard Weed)

Tags

His tag

Expression

Bacterial

Promoter

T7

Available Since

June 17, 2013

Availability

Academic Institutions and Nonprofits only
pMON-HsNMT2

Plasmid

#67476

Purpose

Expression of human N-myristoyltransferase 2 for N-myristoylation of ARF family proteins in bacteria

Depositor

Richard Kahn

Article

Van Valkenburgh et al Methods Enzymol. 2002;344:186-93.

Insert

HsNMT2 (NMT2 Human)

Expression

Bacterial

Available Since

Oct. 5, 2015

Availability

Academic Institutions and Nonprofits only
pGL2-ptc-lu

Plasmid

#37678

Depositor

Philip Beachy

Article

Chen et al Cell. 1999 Aug 6;98(3):305-16.

Insert

ptc promoter (−758 to +130)

Use

Luciferase

Tags

luciferase

Expression

Insect

Promoter

ptc

Available Since

Nov. 12, 2012

Availability

Academic Institutions and Nonprofits only
pCS2 xenopus Insulin 2

Plasmid

#25786

Depositor

Edward De Robertis

Article

Pera et al Mech Dev. 2002 Aug . 116(1-2):169-72.

Insert

Insulin 2

Expression

Mammalian

Available Since

July 1, 2010

Availability

Academic Institutions and Nonprofits only