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MCV-HF LTS220A/S239A

Plasmid

#112191

Purpose

Merkel cell polyomavirus molecular clone

Depositor

Patrick Moore

Article

Kwun et al Proc Natl Acad Sci U S A. 2017 May 16;11

Insert

MCV-HF LTS220A/S239A

Mutation

T to G mutations at 3313 (nt) and 3370 (nt) of fu…

Promoter

None from vector. Insert contains native promoter…

Available Since

Feb. 11, 2019

Availability

Academic Institutions and Nonprofits only

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pLG1-puro-sgATL3-5

Plasmid

#109011

Purpose

CRISPRi knockdown of targeted gene (to be used with Addgene #102244 or other dCas9-KRAB constructs)

Depositor

Jacob Corn

Article

Liang et al J Cell Biol. 2018 Aug 24. pii: jcb.20180

Insert

ATL3-sgRNA #5 (ATL3 Human)

Use

Lentiviral

Expression

Mammalian

Promoter

mU6 promoter

Available Since

Aug. 22, 2018

Availability

Academic Institutions and Nonprofits only

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pENTR-L3-eSpCas9n(1.1)-2A-EGFP-L2

Plasmid

#141052

Purpose

Mutisite Gateway mediated vector construction

Depositor

Hideyuki Okano

Article

Yoshimatsu et al PLoS One. 2019 Aug 27;14(8):e0221164. do

Insert

eSpCas9(1.1)-2A-EGFP

Expression

Mammalian

Available Since

July 8, 2020

Availability

Academic Institutions and Nonprofits only

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pconst #2-RFP

Plasmid

#81076

Purpose

constitutive promoter expressing RFP, biobrick construction

Depositor

Paul Adams, Jay Keasling

Article

Dahl et al Nat Biotechnol. 2013 Nov;31(11):1039-46.

Insert

RFP

Use

P15a origin, constitutive promoter, kanr

Promoter

Constitutive Promoter- BBa_J23104

Available Since

Aug. 18, 2016

Availability

Academic Institutions and Nonprofits only

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pLz74_Rubisco_CsoS2_Npeptide_fusion

Plasmid

#140840

Purpose

Same as pBz15 but with N*-peptide fusion on CbbL, construct used for protein crystallization

Depositor

David Savage

Article

Oltrogge et al Nat Struct Mol Biol. 2020 Mar;27(3):281-

Insert

Form IA Rubisco with CsoS2 N-peptide fusion; CbbL-Npeptide, CbbS

Tags

CsoS2 N-peptide consensus to CbbL C-terminus and …

Expression

Bacterial

Promoter

T7

Available Since

May 8, 2020

Availability

Academic Institutions and Nonprofits only

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pIhh715bp-Luc

Plasmid

#53606

Purpose

Reporter construct containing a 715 bp promoter region of the Ihh gene in front of a luciferase gene for in vitro DNA transfection assays

Depositor

Xiangli Yang

Article

Wang et al Development. 2009 Dec;136(24):4143-53. d

Insert

Ihh promoter

Use

Luciferase; Reporter

Available Since

June 23, 2014

Availability

Academic Institutions and Nonprofits only
myc-SpELL pBacPAK8

Plasmid

#15318

Depositor

Joan Conaway, Ronald Conaway

Article

Banks et al J Biol Chem. 2007 Feb 23. 282(8):5761-9.

Insert

eleven nineteen lysine rich in leukemia (ell1 Fission Yeast)

Use

Baculovirus construction

Tags

Myc

Available Since

Aug. 15, 2007

Availability

Academic Institutions and Nonprofits only
myc-SpEAF pBacPAK8

Plasmid

#15320

Depositor

Joan Conaway, Ronald Conaway

Article

Banks et al J Biol Chem. 2007 Feb 23. 282(8):5761-9.

Insert

ELL associated factor (eaf1 Fission Yeast)

Use

Baculovirus construction

Tags

Myc

Available Since

Aug. 15, 2007

Availability

Academic Institutions and Nonprofits only
myc-EAF pBacPAK8

Plasmid

#15324

Depositor

Joan Conaway, Ronald Conaway

Article

Kong et al Proc Natl Acad Sci U S A. 2005 Jul 19. 1

Insert

ELL Associated Factor (EAF1 Human)

Use

Baculovirus construction

Tags

Myc

Available Since

Aug. 15, 2007

Availability

Academic Institutions and Nonprofits only
p072 pCS xGATA4 + xTRPS1(C119)

Plasmid

#11043

Depositor

Ramesh Shivdasani

Article

Malik et al EMBO J. 2001 Apr 2. 20(7):1715-25.

Insert

GATA4 + TRPS1(C119) (trps1, gata4-b Frog)

Use

Xenopus, mammalian, avian, and zebrafish

Mutation

Fusion construct of XGATA4 (amino acids 1-352) wi…

Available Since

Dec. 6, 2005

Availability

Academic Institutions and Nonprofits only

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pA-Opto

Plasmid

#253255

Purpose

Opto-CheY expressed using the proA promoter and T7 RBS variant GAAGGAGgT

Depositor

Aravinthan DT Samuel

Article

Vrabioiu et al Proc Natl Acad Sci U S A. 2026 Mar 10;12

Insert

Opto-CheY

Expression

Bacterial

Mutation

T7 RBS variant GAAGGAGgT

Available Since

April 6, 2026

Availability

Academic Institutions and Nonprofits only

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p5g-Opto_N449S

Plasmid

#253285

Purpose

Opto-CheY N449S expressed using the pro5 promoter and T7 RBS variant GAAGGAGgT

Depositor

Aravinthan DT Samuel

Article

Vrabioiu et al Proc Natl Acad Sci U S A. 2026 Mar 10;12

Insert

Opto-CheY with N449S mutation

Tags

T7 RBS variant GAAGGAGgT

Expression

Bacterial

Available Since

April 6, 2026

Availability

Academic Institutions and Nonprofits only

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pA_higher-Opto_N449S

Plasmid

#253258

Purpose

Opto-CheY N449S expressed using the proA promoter and wild type T7 RBS

Depositor

Aravinthan DT Samuel

Article

Vrabioiu et al Proc Natl Acad Sci U S A. 2026 Mar 10;12

Insert

Opto-CheY with N449S mutation

Tags

wild type T7 RBS

Expression

Bacterial

Available Since

March 19, 2026

Availability

Academic Institutions and Nonprofits only

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pA-Opto_N449S

Plasmid

#253256

Purpose

Opto-CheY N449S expressed using the proA promoter and T7 RBS variant GAAGGAGgT

Depositor

Aravinthan DT Samuel

Article

Vrabioiu et al Proc Natl Acad Sci U S A. 2026 Mar 10;12

Insert

Opto-CheY with N449S mutation

Tags

T7 RBS variant GAAGGAGgT

Expression

Bacterial

Available Since

March 19, 2026

Availability

Academic Institutions and Nonprofits only

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pA-Opto_V416T

Plasmid

#253257

Purpose

Opto-CheY V416T expressed using the proA promoter and T7 RBS variant GAAGGAGgT

Depositor

Aravinthan DT Samuel

Article

Vrabioiu et al Proc Natl Acad Sci U S A. 2026 Mar 10;12

Insert

Opto-CheY with V416T mutation

Expression

Bacterial

Mutation

T7 RBS variant GAAGGAGgT

Available Since

March 19, 2026

Availability

Academic Institutions and Nonprofits only

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pDEST-GBKT7

Plasmid

#248655

Purpose

Empty vector for generating yeast 2-hybrid GAL4 DNA binding domain fusion constructs by Gateway cloning. It may also be used as an empty vector experimental control.

Depositor

Sarah Assmann, John Doonan

Article

Rossignol et al J Cell Sci. 2007 Oct 15;120(Pt 20):3678-

Type

Empty backbone

Use

Yeast 2-hybrid

Tags

GAL4 binding domain, Myc

Expression

Yeast

Available Since

Jan. 7, 2026

Availability

Academic Institutions and Nonprofits only

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pDEST-GADT7

Plasmid

#248657

Purpose

Empty vector for generating yeast 2-hybrid GAL4 activation domain fusion constructs by Gateway cloning. It may also be used as an empty vector experimental control.

Depositor

Sarah Assmann, John Doonan

Article

Rossignol et al J Cell Sci. 2007 Oct 15;120(Pt 20):3678-

Type

Empty backbone

Use

Yeast 2-hybrid

Tags

GAL4 activation domain, HA

Expression

Yeast

Available Since

Jan. 7, 2026

Availability

Academic Institutions and Nonprofits only

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pCMV-COX8 MTS-3xFlag-TALE array-CBE6d-UGI-P2A-mCherry-UTR

Plasmid

#235202

Purpose

Plasmids for constructing mitoBEs v2

Depositor

Wensheng Wei

Article

Zhang et al Nature. 2025 Jan 22. doi: 10.1038/s41586

Insert

COX8 MTS-3xFlag-TALE array-CBE6d-UGI-P2A-mCherry-UTR

Expression

Mammalian

Available Since

Sept. 17, 2025

Availability

Academic Institutions and Nonprofits only

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Px-PhiYFP-triplex-28-M13-28-pA

Plasmid

#202047

Purpose

Encodes PhiYFP downstream of a cloning site for constructing a synthetic Cas-targeted promoter. 3’ UTR has a MALAT1 triplex, Csy4 28nt sites, and cloning site for gRNA to activate downstream targets.

Depositor

Darren Cusanovich

Article

Kang et al ACS Synth Biol. 2025 May 16;14(5):1732-1

Insert

PhiYFP

Expression

Mammalian

Available Since

Aug. 27, 2025

Availability

Academic Institutions and Nonprofits only

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Px-PhiYFP-triplex-DR-M13-DR-pA

Plasmid

#202046

Purpose

Encodes PhiYFP downstream of a cloning site for constructing a synthetic Cas-targeted promoter. 3’ UTR has a MALAT1 triplex, dCas12a DRs, and cloning site for crRNA to activate downstream targets.

Depositor

Darren Cusanovich

Article

Kang et al ACS Synth Biol. 2025 May 16;14(5):1732-1

Insert

PhiYFP

Expression

Mammalian

Available Since

Aug. 27, 2025

Availability

Academic Institutions and Nonprofits only

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