We narrowed to 7,126 results for: crispr cas9 plasmids
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miniCGBE1-SpRY (pBM1571)
Plasmid#170105PurposeCMV promoter expression plasmid for rAPOBEC1(R33A)-SpRY-nCas9(D10A)-P2A-EGFPDepositorInsertrAPOBEC1(R33A)-SpRY-nCas9(D10A)-P2A-EGFP
UseCRISPRTagsP2A-EGFPExpressionMammalianMutationR33A in rAPOBEC1 and SpRY-nCas9 (D10A/A61R/L1111R…PromoterCMVAvailable SinceMay 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
pX330 p53
Plasmid#59910PurposepX330 backbone expressing sgRNA targeting p53 to edit mouse p53. Expresses Cas9 from CBh promoterDepositorAvailable SinceMarch 16, 2015AvailabilityAcademic Institutions and Nonprofits only -
pX330 Pten
Plasmid#59909PurposepX330 backbone expressing sgRNA targeting Pten to edit mouse Pten. Expresses Cas9 from CBh promoterDepositorAvailable SinceMarch 9, 2015AvailabilityAcademic Institutions and Nonprofits only -
pX330 Ctnnb1.1
Plasmid#59911PurposepX330 backbone expressing sgRNA targeting Ctnnb1 to edit mouse beta-Catenin. Expresses Cas9 from CBh promoterDepositorAvailable SinceMarch 9, 2015AvailabilityAcademic Institutions and Nonprofits only -
pYDE009
Plasmid#194151PurposeDelivery of dcas9 into A. baumannii via Tn7 for CRISPRi. Tn7 element has tetR-tetP-dcas9, GmR; plasmid backbone is CarbR.DepositorInserttetR-tetP-dcas9-rrnB T1-T7Te
ExpressionBacterialPromotertetpAvailable SinceFeb. 7, 2023AvailabilityAcademic Institutions and Nonprofits only -
pNuc-cis
Plasmid#202796PurposeRK2-based conjugative plasmid containing the TevSpCas9 dual-endonuclease for targeted bacterial killingDepositorInsertTevSpCas9, sgRNA cassette, oriT, CenArsHis
ExpressionBacterialAvailable SinceAug. 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAAV-SlugABE-NNG
Plasmid#214356PurposeExpresses SlugABE-NNGDepositorInsertSlugABE-NNG
UseAAVExpressionMammalianMutationQ782R/S888R/L906R/N984S/E1012K/K1016IAvailable SinceApril 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
pSCL.72
Plasmid#184984PurposeExpress Cas9/-Eco1 RT fusion, using linker1DepositorInsertCas9-fusion_linker1-Eco1RT
TagsSV40NLSExpressionYeastMutationhuman codon optimized RTPromoterGal1-10Available SinceNov. 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
pSCL.95
Plasmid#184986PurposeExpress Cas9/-Eco1 RT fusion, using linker2DepositorInsertCas9-fusion_linker2-Eco1RT
TagsSV40NLSExpressionYeastMutationhuman codon optimized RTPromoterGal1-10Available SinceNov. 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
pSCL.94
Plasmid#184985PurposeExpress -Eco1 RT/Cas9 fusion, using linker2DepositorInsertEco1RT-fusion_linker2-Cas9
TagsSV40NLSExpressionYeastMutationhuman codon optimized RTPromoterGal1-10Available SinceNov. 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
pSCL.71
Plasmid#184983PurposeExpress -Eco1 RT/Cas9 fusion, using linker1DepositorInsertEco1RT-fusion_linker1-Cas9
TagsSV40NLSExpressionYeastMutationhuman codon optimized RTPromoterGal1-10Available SinceNov. 2, 2022AvailabilityAcademic Institutions and Nonprofits only -
pTubb3-MC
Plasmid#87112Purposeminicircle parental backbone plasmid including GFP knock-in donor targeting Tubb3 gene and one cutting site for HITIDepositorInsertGFP
UseCRISPRAvailable SinceMarch 31, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCMV-PE2-SpG (HES824)
Plasmid#242689PurposePrime editing in mammalian cells with SpG-based PE2 prime editorsDepositorInsertpCMV-NLS-SpCas9(H840A)-M-MLV-RT(D200N,T306K,W313F,T330P,L603W)-NLS
UseCRISPRTagsNLSExpressionMammalianMutationPE2 mutations in MMLV RT, SpG mutations in SpCas9…PromoterCMVAvailable SinceOct. 9, 2025AvailabilityAcademic Institutions and Nonprofits only -
pX330-PITCh-TUBA1B
Plasmid#207763PurposeExpresses SpCas9, the PITCh gRNA, and a sgRNA targeting the N-terminus of TUBA1B for knock-in.DepositorInsertsgRNA Targeting N-terminus of TUBA1B (TUBA1B Human)
UseCRISPRExpressionMammalianPromoterU6Available SinceNov. 29, 2023AvailabilityAcademic Institutions and Nonprofits only -
pX330-CENPA
Plasmid#227282PurposeExpresses SpCas9 and a sgRNA targeting the N-terminus of CENPA for knock-in.DepositorInsertsgRNA Targeting N-terminus of CENPA (CENPA Human)
UseCRISPRExpressionMammalianPromoterU6Available SinceNov. 6, 2024AvailabilityAcademic Institutions and Nonprofits only
