We narrowed to 20,028 results for: cas9 genes

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lentiCRISPRv2-7SK-Blast

Plasmid

#183620

Purpose

lentiCRISPRv2 derivative with modified 7SK promoter driving sgRNA and encoding Blasticidin resistance. Compatible with inserts designed for lentiCRISPRv2.

Depositor

Bill Sugden

Article

Hutcheson et al ACS Synth Biol. 2022 Jun 29. doi: 10.102

Type

Empty backbone

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

Modified 7SK

Available Since

July 13, 2022

Availability

Academic Institutions and Nonprofits only

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pDD397

Plasmid

#91822

Purpose

AID FP-slot donor for the SapTrap cloning system

Depositor

Bob Goldstein

Article

Dickinson et al MicroPubl Biol. 2018 May 1;2018:10.17912

Insert

Auxin-inducible degron (AID)

Use

CRISPR

Expression

Worm

Available Since

June 23, 2017

Availability

Academic Institutions and Nonprofits only

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YN2_1_LT84_RPL13A

Plasmid

#177712

Purpose

Expresses Cas9 and sgRNA cassette for targeting RPL13A in yeast Saccharomyces cerevisiae

Depositor

Lisbeth Olsson

Article

Torello Pianale et al Front Microbiol. 2022 Jan 7;12:802169. d

Insert

Cas9

Use

CRISPR

Expression

Yeast

Available Since

Jan. 13, 2022

Availability

Academic Institutions and Nonprofits only

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pDD398

Plasmid

#91832

Purpose

AID NT-tag linker donor for the SapTrap cloning system

Depositor

Bob Goldstein

Article

Dickinson et al MicroPubl Biol. 2018 May 1;2018:10.17912

Insert

Auxin-inducible degron (AID)

Use

CRISPR

Expression

Worm

Available Since

June 23, 2017

Availability

Academic Institutions and Nonprofits only

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pAP685-2

Plasmid

#99490

Purpose

TEV::eGFP::ER::6xHis::linker::3XFlag

Depositor

Geraldine Seydoux

Article

Paix et al Methods. 2017 May 15;121-122:86-93. doi:

Insert

TEV::eGFP::RE::6Xhis::linker::3XFlag

Expression

Bacterial

Available Since

March 1, 2018

Availability

Academic Institutions and Nonprofits only

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pCfB8382

Plasmid

#126916

Purpose

Plasmid containing integration cassette at XI-3 integration site in Saccharomyces cerevisiae for overexpression of XdCrtI

Depositor

Irina Borodina

Article

Milne et al FEMS Yeast Res. 2019 Sep 26. pii: foz062

Insert

XdCrtI

Expression

Yeast

Available Since

Dec. 17, 2019

Availability

Academic Institutions and Nonprofits only

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pCF525-AcrIIA13b_gb76_GF31-2-hom_lenti

Plasmid

#138310

Purpose

Lenti expression of AcrIIA13b

Depositor

Jennifer Doudna

Article

Watters et al Proc Natl Acad Sci U S A. 2020 Mar 10. p

Insert

AcrIIA13b

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

March 17, 2020

Availability

Academic Institutions and Nonprofits only

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pCF525-AcrIIA15_gb79_Cand27_lenti

Plasmid

#138313

Purpose

Lenti expression of AcrIIA15

Depositor

Jennifer Doudna

Article

Watters et al Proc Natl Acad Sci U S A. 2020 Mar 10. p

Insert

AcrIIA15

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

March 17, 2020

Availability

Academic Institutions and Nonprofits only

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pCF525-AcrIIA14_gb77_Cand9_lenti

Plasmid

#138311

Purpose

Lenti expression of AcrIIA14

Depositor

Jennifer Doudna

Article

Watters et al Proc Natl Acad Sci U S A. 2020 Mar 10. p

Insert

AcrIIA14

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

March 13, 2020

Availability

Academic Institutions and Nonprofits only

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pAP639-2F

Plasmid

#99486

Purpose

3XFlag::TEV::6XHis::mTurquoise::6XHis::TEV::3XFlag

Depositor

Geraldine Seydoux

Article

Paix et al Methods. 2017 May 15;121-122:86-93. doi:

Insert

3XFlag::TEV::6XHis::mTurquoise::6XHis::TEV::3XFlag

Expression

Bacterial

Available Since

March 1, 2018

Availability

Academic Institutions and Nonprofits only

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pAP683-2

Plasmid

#99488

Purpose

TEV::eGFP::ER::HA::linker::3XFlag

Depositor

Geraldine Seydoux

Article

Paix et al Methods. 2017 May 15;121-122:86-93. doi:

Insert

TEV::eGFP::RE::HA::linker::3XFlag

Expression

Bacterial

Available Since

Sept. 8, 2017

Availability

Academic Institutions and Nonprofits only

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pAP607-2

Plasmid

#99483

Purpose

Myc::TEV::3XFlag::GFP::3XFlag::TEV::Myc

Depositor

Geraldine Seydoux

Article

Paix et al Methods. 2017 May 15;121-122:86-93. doi:

Insert

Myc::TEV::3XFlag::GFP::3XFlag::TEV::Myc

Expression

Bacterial

Available Since

Sept. 5, 2017

Availability

Academic Institutions and Nonprofits only

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pWT019b

Plasmid

#107894

Purpose

W1.2 plasmid. Contains PTetO-sd2U-Cas9, PLacO-sgRNA1, PRha-sgRNA2 (sgRNA1-G19T-G20C) and is part of CAMERA 1.2

Depositor

David Liu

Article

Tang et al Science. 2018 Feb 15. pii: science.aap89

Insert

PTetO-sd2U-Cas9, PLacO-sgRNA1, PRha-sgRNA2 (sgRNA1-G19T-G20C)

Expression

Bacterial

Available Since

April 17, 2018

Availability

Academic Institutions and Nonprofits only

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pAP684-3

Plasmid

#99489

Purpose

TEV::eGFP::ER::Ollas::linker::3XFlag

Depositor

Geraldine Seydoux

Article

Paix et al Methods. 2017 May 15;121-122:86-93. doi:

Insert

TEV::eGFP::RE::Ollas::linker::3XFlag

Expression

Bacterial

Available Since

Sept. 7, 2017

Availability

Academic Institutions and Nonprofits only

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pJAT22

Plasmid

#204311

Purpose

For generating targeting inversions. N-terminal split GFP, expressed in eyes.

Depositor

David Stern

Article

Stern et al bioRxiv 2023.06.17.545412

Insert

3XP3-GFPn

Use

CRISPR

Promoter

3XP3

Available Since

March 20, 2025

Availability

Academic Institutions and Nonprofits only

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pUC57-ITR2-H1-sgCTG-SlugD10A-3XFLAG-SV40 NLS-ITR2

Plasmid

#222857

Purpose

This plasmid codes for the Slug-KH nickase with a sg(CTG)6

Depositor

Vincent Dion

Article

Mangin et al bioRxiv 2023.10.24.563270

Insert

Slug-KHD10A+sgCTG

Use

CRISPR

Tags

3X FLAG, SV40 NLS, ITR2 and ITR2

Expression

Mammalian

Mutation

KH variant and D10A mutation

Promoter

H1 and eCMV

Available Since

Oct. 17, 2024

Availability

Academic Institutions and Nonprofits only

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pUC57-ITR2-H1-sgAGC-SlugD10A-3XFLAG-SV40 NLS-ITR2

Plasmid

#222859

Purpose

This plasmid codes for the Slug-KH nickase with a sg(AGC)6

Depositor

Vincent Dion

Article

Mangin et al bioRxiv 2023.10.24.563270

Insert

Slug-KHD10A+sgAGC

Use

CRISPR

Tags

3X FLAG, SV40 NLS, ITR2 and ITR2

Expression

Mammalian

Mutation

KH variant and D10A mutation

Promoter

H1 and eCMV

Available Since

Oct. 17, 2024

Availability

Academic Institutions and Nonprofits only

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pUC57-ITR2-H1-sgCAG-SlugD10A-3XFLAG-SV40 NLS-ITR2

Plasmid

#222858

Purpose

This plasmid codes for the Slug-KH nickase with a sg(CAG)6

Depositor

Vincent Dion

Article

Mangin et al bioRxiv 2023.10.24.563270

Insert

Slug-KHD10A+sgCAG

Use

CRISPR

Tags

3X FLAG, SV40 NLS, ITR2 and ITR2

Expression

Mammalian

Mutation

KH variant and D10A mutation

Promoter

H1 and eCMV

Available Since

Oct. 17, 2024

Availability

Academic Institutions and Nonprofits only

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pUC57-ITR2-H1-sgGCA-SlugD10A-3XFLAG-SV40 NLS-ITR2

Plasmid

#222860

Purpose

This plasmid codes for the Slug-KH nickase with a sg(GCA)6

Depositor

Vincent Dion

Article

Mangin et al bioRxiv 2023.10.24.563270

Insert

Slug-KHD10A+sgGCA

Use

CRISPR

Tags

3X FLAG, SV40 NLS, ITR2 and ITR2

Expression

Mammalian

Mutation

KH variant and D10A mutation

Promoter

H1 and eCMV

Available Since

Oct. 17, 2024

Availability

Academic Institutions and Nonprofits only

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pKEW395_AcrIIA14_truncated

Plasmid

#138284

Purpose

Expression of truncated AcrIIA14 protein

Depositor

Jennifer Doudna

Article

Watters et al Proc Natl Acad Sci U S A. 2020 Mar 10. p

Insert

truncated AcrIIA14

Use

CRISPR

Tags

MBP

Expression

Bacterial

Available Since

March 23, 2020

Availability

Academic Institutions and Nonprofits only

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