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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 32,661 results for: Eng

Showing: 6461 - 6480 of 32661 results

  1. TRUPATH Triple GaoA

    Plasmid
    #196051
    Purpose
    Encodes a G alpha subunit (GNAO1 Isoform Alpha 1) with RLuc8, a G gamma subunit (GNG8) with GFP2 and a G beta subunit (GNB3) as optimal components of a BRET2 biosensor
    Depositor
    Justin English
    Article
    Olsen et al Nat Chem Biol. 2020 May 4. pii: 10.1038/
    Inserts
    GAlphaoA-RLuc8
    GGamma8-GFP2 (GNG8 Human)
    GBeta3 (GNB3 Human)
    Use
    Luciferase
    Tags
    GFP2 and GSAG linker
    Expression
    Mammalian
    Mutation
    RLuc8 and flanking SGGGGS linkers have been inser…
    Promoter
    CMV
    Available Since
    Feb. 27, 2023
    Availability
    Academic Institutions and Nonprofits only
    196051-plasmid-map-sequence-id-385865

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  4. pVRC8400-SARS-CoV-2-RBD-XBB.1.5-AVI

    Plasmid
    #198466
    Purpose
    Expresses SARS-CoV-2 RBD protein from the XBB.1.5 variant with single chain Fc, HRV3C protease cleavage site, and AVI tag
    Depositor
    Peter Kwong
    Article
    Teng et al PLoS One. 2022 May 24;17(5):e0268767. do
    Insert
    SARS-CoV-2-RBD-XBB.1.5 (S )
    Tags
    AVI, HRV3C, and scFc
    Expression
    Mammalian
    Mutation
    G339H, R346T, L368I, S371F, S373P, S375F, T376A, …
    Available Since
    March 21, 2023
    Availability
    Academic Institutions and Nonprofits only
    198466-plasmid-map-sequence-id-388113

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  6. pVRC8400-SARS-CoV-2-S2P-B.1.1.529-AVI

    Plasmid
    #181695
    Purpose
    Expresses SARS-CoV-2 S2P protein from the B.1.1.529 (Omicron) variant with single chain Fc, HRV3C protease cleavage site, and AVI tag
    Depositor
    Peter Kwong
    Article
    Teng et al PLoS One. 2022 May 24;17(5):e0268767. do
    Insert
    SARS-CoV-2-S2P-B.1.1.529 (S )
    Tags
    AVI, HRV3C, and scFc
    Expression
    Mammalian
    Mutation
    A67V, ∆H69, ∆V70, T95I, G142D, ∆V143, ∆Y144, ∆Y14…
    Available Since
    March 2, 2022
    Availability
    Academic Institutions and Nonprofits only
    181695-plasmid-map-sequence-id-354495

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  9. pBOBI C-MYC GAC F7

    Plasmid
    #227828
    Purpose
    To amplify the virus that expresses C-MYC GAC F7 with 33 residues mutated to alanine at the interface for interacting with PDZD8
    Depositor
    Sheng-cai Lin
    Article
    Li et al Cell Res. 2024 Jun 19. doi: 10.1038/s414
    Insert
    GAC isoform of F7 (GLS Human)
    Use
    Lentiviral
    Tags
    MYC
    Expression
    Mammalian
    Mutation
    P137, L139, E140, L142, Y145, G150, Q151, E152, K…
    Available Since
    Oct. 8, 2025
    Availability
    Academic Institutions and Nonprofits only
    227828-plasmid-map-sequence-id-488851

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  10. pGEX GAC F7

    Plasmid
    #227829
    Purpose
    To express in E. coli GAC F7 with 33 residues mutated to alanine at the interface for interacting with PDZD8
    Depositor
    Sheng-cai Lin
    Article
    Li et al Cell Res. 2024 Jun 19. doi: 10.1038/s414
    Insert
    GAC isoform of F7 (GLS Human)
    Tags
    GST
    Expression
    Bacterial
    Mutation
    P137, L139, E140, L142, Y145, G150, Q151, E152, K…
    Available Since
    July 23, 2025
    Availability
    Academic Institutions and Nonprofits only
    227829-plasmid-map-sequence-id-479453

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  11. pHM1 KGA-33A

    Plasmid
    #227836
    Purpose
    Used for microinjection of nematodes for expression of KGA-33A, GLS with 33 residues mutated to alanine at the interface for interacting with PDZD8
    Depositor
    Sheng-cai Lin
    Article
    Li et al Cell Res. 2024 Jun 19. doi: 10.1038/s414
    Insert
    KGA isoform (GLS Human)
    Tags
    GFP
    Expression
    Worm
    Mutation
    P137, L139, E140, L142, Y145, G150, Q151, E152, K…
    Available Since
    July 23, 2025
    Availability
    Academic Institutions and Nonprofits only
    227836-plasmid-map-sequence-id-479443

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  12. pGEX KGA F7

    Plasmid
    #227822
    Purpose
    To express in E. coli KGA F7 with 33 residues mutated to alanine at the interface for interacting with PDZD8
    Depositor
    Sheng-cai Lin
    Article
    Li et al Cell Res. 2024 Jun 19. doi: 10.1038/s414
    Insert
    KGA isoform of F7 (GLS Human)
    Tags
    GST
    Expression
    Bacterial
    Mutation
    P137, L139, E140, L142, Y145, G150, Q151, E152, K…
    Available Since
    June 30, 2025
    Availability
    Academic Institutions and Nonprofits only
    227822-plasmid-map-sequence-id-477347

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  13. pBOBI C-MYC KGA F7

    Plasmid
    #227821
    Purpose
    To amplify the virus that expresses C-MYC KGA F7 with 33 residues mutated to alanine at the interface for interacting with PDZD8
    Depositor
    Sheng-cai Lin
    Article
    Li et al Cell Res. 2024 Jun 19. doi: 10.1038/s414
    Insert
    KGA isoform of F7 (GLS Human)
    Use
    Lentiviral
    Tags
    MYC
    Expression
    Mammalian
    Mutation
    P137, L139, E140, L142, Y145, G150, Q151, E152, K…
    Available Since
    June 27, 2025
    Availability
    Academic Institutions and Nonprofits only
    227821-plasmid-map-sequence-id-477304

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  16. pVRC8400-SARS-CoV-2-S2P-B.1.617-G142D-E154K-L452R-E484Q-D614G-P681R-Q1071H-H1101D-AVI

    Plasmid
    #176332
    Purpose
    Expresses SARS-CoV-2 S2P protein from the B.1.617 variant containing G142D-E154K-L452R-E484Q-D614G-P681R-Q1071H-H1101D mutations with single chain Fc, HRV3C protease cleavage site, and AVI tag
    Depositor
    Peter Kwong
    Article
    Teng et al PLoS One. 2022 May 24;17(5):e0268767. do
    Insert
    SARS-CoV-2-S2P-B.1.617-G142D-E154K-L452R-E484Q-D614G-P681R-Q1071H-H1101D (S )
    Tags
    AVI, HRV3C, and scFc
    Expression
    Mammalian
    Mutation
    G142D, E154K, L452R, E484Q, D614G, P681R, Q1071H,…
    Available Since
    March 2, 2022
    Availability
    Academic Institutions and Nonprofits only
    176332-plasmid-map-sequence-id-347141

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  18. TRUPATH Triple Gai2

    Plasmid
    #196049
    Purpose
    Encodes a G alpha subunit (GNAl2) with RLuc8, a G gamma subunit (GNG8) with GFP2 and a G beta subunit (GNB3) as optimal components of a BRET2 biosensor for studying heterotrimeric G proteins
    Depositor
    Justin English
    Article
    Olsen et al Nat Chem Biol. 2020 May 4. pii: 10.1038/
    Inserts
    GAlphai2-RLuc8 (GNAI2 Human)
    GGamma8-GFP2 (GNG8 Human)
    GBeta3 (GNB3 Human)
    Use
    Luciferase
    Tags
    GFP2 and GSAG linker
    Expression
    Mammalian
    Mutation
    RLuc8 and flanking SGGGGS linkers have been inser…
    Promoter
    CMV
    Available Since
    Feb. 27, 2023
    Availability
    Academic Institutions and Nonprofits only
    196049-plasmid-map-sequence-id-385863

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  19. TRUPATH Triple Ga11

    Plasmid
    #196059
    Purpose
    Encodes a G alpha subunit (GNA11) with RLuc8, a G gamma subunit (GNG13) with GFP2 and a G beta subunit (GNB3) as optimal components of a BRET2 biosensor for studying heterotrimeric G proteins
    Depositor
    Justin English
    Article
    Olsen et al Nat Chem Biol. 2020 May 4. pii: 10.1038/
    Inserts
    GAlpha11-RLuc8 (GNA11 Human)
    GGamma13-GFP2 (GNG13 Human)
    GBeta3 (GNB3 Human)
    Use
    Luciferase
    Tags
    GFP2 and GSAG linker
    Expression
    Mammalian
    Mutation
    RLuc8 and flanking SGGGGS linkers have been inser…
    Promoter
    CMV
    Available Since
    Feb. 28, 2023
    Availability
    Academic Institutions and Nonprofits only
    196059-plasmid-map-sequence-id-386126

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  20. TRUPATH Triple Ga15

    Plasmid
    #196060
    Purpose
    Encodes a G alpha subunit (GNA15) with RLuc8, a G gamma subunit (GNG13) with GFP2 and a G beta subunit (GNB3) as optimal components of a BRET2 biosensor for studying heterotrimeric G proteins
    Depositor
    Justin English
    Article
    Olsen et al Nat Chem Biol. 2020 May 4. pii: 10.1038/
    Inserts
    GAlpha15-RLuc8 (GNA15 Human)
    GGamma13-GFP2 (GNG13 Human)
    GBeta3 (GNB3 Human)
    Use
    Luciferase
    Tags
    GFP2 and GSAG linker
    Expression
    Mammalian
    Mutation
    RLuc8 and flanking SGGGGS linkers have been inser…
    Promoter
    CMV
    Available Since
    Feb. 27, 2023
    Availability
    Academic Institutions and Nonprofits only
    196060-plasmid-map-sequence-id-385870

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Showing: 6461 - 6480 of 32661 results