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pTS35.41

Plasmid

#106263

Purpose

Polycistronic expression of luc, ekivR using P2A

Depositor

Vera Meyer

Article

Schuetze et al Microb Cell Fact. 2017 Sep 25;16(1):162.

Insert

luc-P2A-ekivR

Use

Luciferase and Synthetic Biology

Promoter

Tet-On

Available Since

March 15, 2018

Availability

Academic Institutions and Nonprofits only

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pMPRAdonor2

Plasmid

#49353

Purpose

minP-luc2 donor plasmid for MPRA library construction

Depositor

Tarjei Mikkelsen

Article

Melnikov et al J Vis Exp. 2014 Aug 17;(90). doi: 10.379

Insert

luc2

Use

Luciferase

Promoter

minP

Available Since

Feb. 24, 2014

Availability

Academic Institutions and Nonprofits only

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RLuc_IRES_FLuc_Pless

Plasmid

#226462

Purpose

Promoterless bicistronic luciferase

Depositor

Dogus Altintas, Paolo Comoglio

Article

Cerqua et al EMBO J. 2025 Jan 7. doi: 10.1038/s44318-

Insert

RLuc IRES FLuc

Expression

Mammalian

Promoter

No promoter

Available Since

Jan. 7, 2025

Availability

Academic Institutions and Nonprofits only

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CBFRE-EGFP

Plasmid

#17705

Depositor

Nicholas Gaiano

Article

Mizutani et al Nature. 2007 Sep 20. 449(7160):351-5.

Insert

CBFRE

Use

Adenoviral

Tags

EGFP

Expression

Mammalian

Available Since

April 15, 2008

Availability

Academic Institutions and Nonprofits only

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HA-Vim

Plasmid

#46315

Depositor

Xiangli Yang

Article

Lian et al J Biol Chem. 2009 Oct 30;284(44):30518-2

Insert

Vimentin (Vim Mouse)

Tags

HA

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 2, 2013

Availability

Academic Institutions and Nonprofits only

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pLV-tTRKRAB-red

Plasmid

#12250

Depositor

Didier Trono

Article

Wiznerowicz et al J Virol. 2003 Aug . 77(16):8957-61.

Insert

TetR-KRAB fusion and dsRED

Use

Cre/Lox, Lentiviral, and RNAi

Expression

Mammalian

Mutation

dsRed added to backbone.

Available Since

Aug. 4, 2006

Availability

Academic Institutions and Nonprofits only

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pET15-UbE2W

Plasmid

#15809

Depositor

Wade Harper

Article

Jin et al Nature. 2007 Jun 28. 447(7148):1135-8.

Insert

Ubiquitin conjugating enzyme UbE2W (UBE2W Human)

Tags

His

Expression

Bacterial

Available Since

Sept. 28, 2007

Availability

Academic Institutions and Nonprofits only

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pDEST17-UbE2Q2

Plasmid

#15801

Depositor

Wade Harper

Article

Jin et al Nature. 2007 Jun 28. 447(7148):1135-8.

Insert

Ubiquitin conjugating enzyme UbE2Q2 (UBE2Q2 Human)

Tags

His

Expression

Bacterial

Available Since

Sept. 28, 2007

Availability

Academic Institutions and Nonprofits only
Pig3 Delta Promoter

Plasmid

#16490

Depositor

Bert Vogelstein

Article

Polyak et al Nature. 1997 Sep 18. 389(6648):300-5.

Insert

PIG3 promoter (TP53I3 Human)

Tags

Luciferase

Expression

Mammalian

Available Since

May 14, 2008

Availability

Academic Institutions and Nonprofits only
Tol2-mpx:Lifeact-Ruby

Plasmid

#45246

Depositor

Anna Huttenlocher

Article

Yoo et al Dev Cell. 2010 Feb 16. 18(2):226-36.

Insert

lifeact-ruby

Use

Zebrafish expression

Promoter

mpx

Available Since

Oct. 29, 2013

Availability

Academic Institutions and Nonprofits only

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pRSET his-eGFP

Plasmid

#113551

Purpose

For bacterial expression and purification of eGFP

Depositor

Jeanne Stachowiak

Article

Busch et al Nat Commun. 2015 Jul 24;6:7875. doi: 10.

Insert

his-eGFP

Expression

Bacterial

Mutation

A206K

Available Since

July 25, 2018

Availability

Academic Institutions and Nonprofits only

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pToneDest

Plasmid

#67691

Purpose

attR4-attR3 Destination cassette in Tol1 backbone

Depositor

Nathan Lawson

Article

Shin et al Development. 2016 Oct 15;143(20):3785-37

Type

Empty backbone

Available Since

Aug. 7, 2015

Availability

Academic Institutions and Nonprofits only

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psiCHECK2-let-7 8x

Plasmid

#20931

Depositor

Yukihide Tomari

Article

Iwasaki et al Mol Cell. 2009 Apr 10;34(1):58-67. doi:

Insert

8x let-7 target sites

Use

Luciferase

Expression

Insect and Mammalian

Available Since

June 19, 2009

Availability

Academic Institutions and Nonprofits only

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pEPkan-S

Plasmid

#41017

Purpose

PCR template for en passant (two-step Red-mediated) mutagenesis

Depositor

Nikolaus Osterrieder

Article

Tischer et al Biotechniques. 2006 Feb;40(2):191-7.

Insert

Kana_I-SceI

Available Since

Jan. 21, 2015

Availability

Academic Institutions and Nonprofits only

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pOK12

Plasmid

#49789

Purpose

E. coli cloning vector (KanR, low copy, blue/white selection)

Depositor

Joachim Messing

Article

Vieira et al Gene. 1991 Apr;100:189-94.

Type

Empty backbone

Use

Cloning vector

Promoter

lacZ

Available Since

June 24, 2015

Availability

Academic Institutions and Nonprofits only

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pBRIT HA/FLAG

Plasmid

#17519

Depositor

Michael Rudnicki

Article

McKinnell et al Nat Cell Biol. 2008 Jan . 10(1):77-84.

Type

Empty backbone

Use

Cre/Lox and Retroviral

Tags

FLAG and HA

Expression

Mammalian

Available Since

March 12, 2008

Availability

Academic Institutions and Nonprofits only
pDEST17-UbE2G2

Plasmid

#15791

Depositor

Wade Harper

Article

Jin et al Nature. 2007 Jun 28. 447(7148):1135-8.

Insert

Ubiquitin conjugating enzyme UbE2G2 (UBE2G2 Human)

Tags

His

Expression

Bacterial

Available Since

Sept. 28, 2007

Availability

Academic Institutions and Nonprofits only

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pMPRAdonor1

Plasmid

#49352

Purpose

Promoterless luc2 donor plasmid for MPRA library construction

Depositor

Tarjei Mikkelsen

Article

Melnikov et al J Vis Exp. 2014 Aug 17;(90). doi: 10.379

Insert

luc2

Use

Luciferase

Promoter

None

Available Since

Feb. 24, 2014

Availability

Academic Institutions and Nonprofits only

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pAC-hRluc

Plasmid

#24348

Depositor

Liqun Luo

Article

Potter et al Cell. 2010 Apr 30. 141(3):536-48.

Insert

hRluc

Expression

Insect

Available Since

March 31, 2010

Availability

Academic Institutions and Nonprofits only

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GST-PRMT1

Plasmid

#34699

Depositor

Steven Clarke

Article

Gary et al J Biol Chem. 1996 May 24;271(21):12585-9

Insert

PRMT1 (Prmt1 Rat)

Tags

GST

Expression

Bacterial

Promoter

tac

Available Since

Jan. 27, 2012

Availability

Academic Institutions and Nonprofits only