We narrowed to 36,673 results for: kan
-
Plasmid#124657PurposePI(3,4,5)P3 probe. PH domain of BTK fused with mRuby2DepositorInsertBTK (BTK Human)
TagsmRuby2ExpressionMammalianMutationcontains only the PH domain of Bruton tyrosine ki…Available SinceMay 24, 2019AvailabilityAcademic Institutions and Nonprofits only -
Myo1E-pEGFP
Plasmid#124658PurposeMouse myosin-1e fused with EGFPDepositorAvailable SinceMay 24, 2019AvailabilityAcademic Institutions and Nonprofits only -
pJL1-SpCas9
Plasmid#117051PurposeIn vitro expression of S. pyogenes Cas9 from the T7 promoterDepositorInsertS. pyogenes Cas9
ExpressionBacterialPromoterT7Available SinceMay 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
SiriusGFP
Plasmid#123201PurposeWe have developed a variant of eGFP, SiriusGFP, that shows over a two fold increase in photostability with utility in methods requiring sustained or high intensity excitation as in 4D confocal or SIM.DepositorInsertSiriusGFP
UseMouse TargetingTagsN/AExpressionBacterial and MammalianMutationeGFP-S30R/Y39N/F99S/N105T/S147R/M153T/V163A/S205V…PromoterCMVAvailable SinceMarch 25, 2019AvailabilityAcademic Institutions and Nonprofits only -
pEG302 22aa SunTag VP64 g4+g17
Plasmid#120252PurposeCRISPR-Cas9 SunTag system to target VP64 to the FWA locus with two guide RNAsDepositorInsertg17_U6_g4_U6_NOS_NLS_GB1_NLS_linker_VP64_linker_sfGFP_scFv_UBQ10_Insulator_UBQ10_Ω_dCas9_1xHA_3xNLS_linker_10xGCN4_OCS
UseCRISPR and Synthetic BiologyExpressionPlantAvailable SinceMarch 17, 2019AvailabilityAcademic Institutions and Nonprofits only -
pGTag-eGFP-B-actin
Plasmid#117814PurposeDonor for precise CRISPR directed genomic integration using the GeneWeld methodDepositorInserteGFP
UseSynthetic BiologyAvailable SinceMarch 15, 2019AvailabilityAcademic Institutions and Nonprofits only -
pET His6 MBP TEV HDAC8_374
Plasmid#122174PurposeExpresses a truncated construct of histone deacetylase 8 for crystallography bearing an N-terminal tobacco etch virus protease-cleavable hexahistidine-maltose binding protein tagDepositorInsertHis6 MBP TEV HDAC8_374 (HDAC8 Human)
Tagshexahistidine tag and tobacco etch virus protease…ExpressionBacterialMutationResidues Met1–Pro7 and H375-V377 have been delete…PromoterT7Available SinceMarch 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
pET28a-mH6-Cas12c1
Plasmid#120872PurposeExpresses E. coli codon optimized Cas12c1 effector in the pET28a backbone.DepositorInsertCas12c1
UseCRISPRTagsmH6ExpressionBacterialMutationWTPromoterlacAvailable SinceFeb. 14, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAcGFP-MALAT1M
Plasmid#120382PurposeReporter for RNA nuclear localizationDepositorAvailable SinceFeb. 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMM643
Plasmid#112533PurposepZE2-PLhrtO-lux-hrtR-RBS3-chuA - HrtR RBS variant of plasmid pMM627 (Strength 33545.5 AU), ColE1 origin, KanRDepositorInsertsHrtR
ChuA
luxCDABE
ExpressionBacterialMutationA17PPromoterJ23107, PL(HrtO), and ProDAvailable SinceFeb. 6, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAC1804_pmax-dCasRx-RBM38
Plasmid#118638PurposeTransient transfection; Expresses dCasRx-RBM38; CAGGS promoterDepositorInsertdCasRx-RBM38
UseCRISPRExpressionMammalianPromoterCAGGSAvailable SinceJan. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pET-28b-T7-hAsCas12a-NLS(nucleoplasmin)-6xHis (BPK3541)
Plasmid#114069PurposeT7 promoter bacterial expression plasmid for human codon optimized AsCas12a with C-terminal NLS and His-tagDepositorInserthuman codon optimized AsCas12a with C-terminal NLS and His-tag
TagsNLS(nucleoplasmin) and 6xHisExpressionBacterialAvailable SinceDec. 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
pEEK2
Plasmid#83492PurposeExpression plasmid for conjugational transfer into cyanobacteriaDepositorTypeEmpty backboneUseSynthetic BiologyTagsStrep-tagExpressionBacterialPromoterPtrc coreAvailable SinceNov. 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
pWaldo-GFPe-SH1446
Plasmid#112507PurposeExpresses SH1446 in E. coli with a C-terminal TEV-GFP-His(8) tag.DepositorInsertPOT family transporter
TagsC terminal TEV GFP octa-His affinity tagExpressionBacterialPromoterT7Available SinceNov. 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
LSB-hsa-miR-21-5p
Plasmid#103345PurposeUsed to sense miRNA activity using fluorescence based tools. hsa-miR-21-5p target in 3' UTR of of mKate. miRNA activity can be measured by the repression of mKate2 relative to EBFP2 fluorescent proteins . Plasmid is inherently unstable; please see Depositor CommentsDepositorAvailable SinceNov. 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLAP-BUBR1
Plasmid#114052Purposetransfection in mammalian cells, combination of fluorescent protein fusion and tandem affinity purificationDepositorInsertBUBR1 (BUB1B Human)
TagsYFP-TEV-S- Human LAPExpressionMammalianMutationC2823A and G2826A (siRNA-resistance)Available SinceNov. 7, 2018AvailabilityAcademic Institutions and Nonprofits only -
pMRE-Tn7-150
Plasmid#118564PurposeminiTn7 plasmid to deliver constitutively expressed fluorescent protein genes in bacteriaDepositorInsertTagBFP2
UseMinitn7 delivery vectorExpressionBacterialAvailable SinceOct. 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
mGarnet2-alpha-tubulin
Plasmid#104318Purposecodes for a fusion of fluorescent protein mGarnet2 and alpha-tubulin, visualizes alpha-tubulinDepositorInsertmGarnet2-alpha-tubulin
ExpressionMammalianPromoterCMVAvailable SinceOct. 25, 2018AvailabilityAcademic Institutions and Nonprofits only -
pEGFP mCherry-ApoB-EGFP
Plasmid#112859PurposePlasmid bearing the editing target of APOBEC1 fused between the mCherry cds and the EGFP one. In presence of APOBEC1, the CAA codon in ApoB is edited to a Stop codon (UAA), leading to loss of EGFPDepositorInsertmCherry-apoB-EGFP
ExpressionMammalianPromoterCMV promoterAvailable SinceOct. 10, 2018AvailabilityAcademic Institutions and Nonprofits only -
pDSG320
Plasmid#115597Purposesurface-displayed Nb2, used in composability experiments; medium copyDepositorInsertregulated adhesin construct
ExpressionBacterialAvailable SinceOct. 5, 2018AvailabilityAcademic Institutions and Nonprofits only