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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 12,189 results for: LEA

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Showing: 6481 - 6500 of 12189 results

  1. Z-FARSB-5

    Plasmid
    #166556
    Purpose
    scFv of a human scaffold targeting phenylalanyl-tRNA synthetase, beta subunit. Antigen coverage aa 1-589 of 589, full-length
    Depositor
    Susanne Gräslund
    Insert
    single chain-Fv, scFv
    Use
    Recombinant antibody expression
    Tags
    3xFLAG, His6 and OmpA leader sequence
    Expression
    Bacterial
    Promoter
    LacZ
    Available since
    April 2, 2021
    Availability
    Academic Institutions and Nonprofits only
    166556-plasmid-map-sequence-id-329264

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  2. J-IRF5-5

    Plasmid
    #166525
    Purpose
    scFv of a human scaffold targeting Interferon regulatory factor 5. Antigen coverage aa E232-L434 of 498
    Depositor
    Susanne Gräslund
    Insert
    single chain-Fv, scFv
    Use
    Recombinant antibody expression
    Tags
    3xFLAG, His6 and OmpA leader sequence
    Expression
    Bacterial
    Promoter
    LacZ
    Available since
    March 31, 2021
    Availability
    Academic Institutions and Nonprofits only
    166525-plasmid-map-sequence-id-329138

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  3. J-SPRTNC-1

    Plasmid
    #166521
    Purpose
    scFv of a human scaffold targeting SprT-like N-terminal domain. Antigen coverage aa 1-216 of 489
    Depositor
    Susanne Gräslund
    Insert
    single chain-Fv, scFv
    Use
    Recombinant antibody expression
    Tags
    3xFLAG, His6 and OmpA leader sequence
    Expression
    Bacterial
    Promoter
    LacZ
    Available since
    March 31, 2021
    Availability
    Academic Institutions and Nonprofits only
    166521-plasmid-map-sequence-id-329135

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  4. G-SP140-8

    Plasmid
    #166520
    Purpose
    scFv of a human scaffold targeting Nuclear body protein SP140. Antigen coverage aa 688-862 of 867, Bromodomain
    Depositor
    Susanne Gräslund
    Insert
    single chain-Fv, scFv
    Use
    Recombinant antibody expression
    Tags
    3xFLAG, His6 and OmpA leader sequence
    Expression
    Bacterial
    Promoter
    LacZ
    Available since
    March 31, 2021
    Availability
    Academic Institutions and Nonprofits only
    166520-plasmid-map-sequence-id-329134

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  5. Z-FUS-5

    Plasmid
    #166561
    Purpose
    scFv of a human scaffold targeting RNA-binding protein FUS. Antigen coverage aa 266-526 of 526
    Depositor
    Susanne Gräslund
    Insert
    single chain-Fv, scFv
    Use
    Recombinant antibody expression
    Tags
    3xFLAG, His6 and OmpA leader sequence
    Expression
    Bacterial
    Promoter
    LacZ
    Available since
    March 24, 2021
    Availability
    Academic Institutions and Nonprofits only
    166561-plasmid-map-sequence-id-328321

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  6. O-FHL1-1

    Plasmid
    #166543
    Purpose
    scFv of a human scaffold targeting Four and a half LIM domains protein 1. Antigen coverage aa 1-280 aa, full-length
    Depositor
    Susanne Gräslund
    Insert
    single chain-Fv, scFv
    Use
    Recombinant antibody expression
    Tags
    3xFLAG, His6 and OmpA leader sequence
    Expression
    Bacterial
    Promoter
    LacZ
    Available since
    March 24, 2021
    Availability
    Academic Institutions and Nonprofits only
    166543-plasmid-map-sequence-id-328279

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  7. A-BRDA4-3

    Plasmid
    #166513
    Purpose
    scFv of a human scaffold targeting Bromodomain-containing protein 4 isoform long. Antigen coverage aa 333-460 of 1362, Bromodomain 2
    Depositor
    Susanne Gräslund
    Insert
    single chain-Fv, scFv
    Use
    Recombinant antibody expression
    Tags
    3xFLAG, His6 and OmpA leader sequence
    Expression
    Bacterial
    Promoter
    LacZ
    Available since
    March 23, 2021
    Availability
    Academic Institutions and Nonprofits only
    166513-plasmid-map-sequence-id-328227

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  9. pCLASP_Crz1(19A)_CLASP_RGS2membrane

    Plasmid
    #133085
    Purpose
    Plasmid contains Crz1* with CLASP. This consists of the plasma membrane anchor (pm-LOVTRAP) and the cargo (Zdk-Crz1*-yeLANS). CLASP modulates nuclear localization of Crz1* in response to blue light.
    Depositor
    Hana El-Samad
    Publication
    Chen et al Cell Syst. 2020 Sep 4. pii: S2405-4712(2
    Insert
    Crz1*-CLASP
    Expression
    Bacterial and Yeast
    Promoter
    pADH1
    Available since
    Sept. 16, 2020
    Availability
    Academic Institutions and Nonprofits only
    133085-plasmid-map-sequence-id-268911

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  13. pOCC110

    Plasmid
    #118905
    Purpose
    shuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with N-terminal HIS6, cleavable with 3C, and C-terminal sortase site (LPETGg)
    Depositor
    Marc Bickle, PEPC MPI CBG
    Publication
    Lemaitre et al BMC Biotechnol. 2019 Mar 29;19(1):20. do
    Insert
    NcoI-HIS6-3C-NotI-ccdB-AscI-LPETGg-stop-HindIII cassette
    Tags
    HIS6, cleavable with 3C protease and LPETGg
    Expression
    Insect
    Promoter
    polH
    Available since
    Jan. 3, 2019
    Availability
    Academic Institutions and Nonprofits only
    118905-plasmid-map-sequence-id-232327

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  14. pOCC207

    Plasmid
    #118869
    Purpose
    shuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with N-terminal HIS6 tag, cleavable with 3C, and N-terminal mCherry
    Depositor
    Marc Bickle, PEPC MPI CBG
    Publication
    Lemaitre et al BMC Biotechnol. 2019 Mar 29;19(1):20. do
    Insert
    NcoI-HIS6-3C-mCherry-NotI-ccdB-AscI-stop-HindIII cassette
    Tags
    HIS6, cleavable with 3C protease, mCherry
    Expression
    Insect
    Promoter
    polH
    Available since
    Jan. 3, 2019
    Availability
    Academic Institutions and Nonprofits only
    118869-plasmid-map-sequence-id-232318

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  15. pOCC215

    Plasmid
    #118879
    Purpose
    shuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with C-terminal Dendra2, and C-terminal HIS6 tag, cleavable with 3C
    Depositor
    Marc Bickle, PEPC MPI CBG
    Publication
    Lemaitre et al BMC Biotechnol. 2019 Mar 29;19(1):20. do
    Insert
    NcoI-NotI-ccdB-AscI-Dendra2-3C-HIS6-stop-HindIII cassette
    Tags
    Dendra2, HIS6, cleavable with 3C protease
    Expression
    Insect
    Promoter
    polH
    Available since
    Jan. 2, 2019
    Availability
    Academic Institutions and Nonprofits only
    118879-plasmid-map-sequence-id-232264

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  19. tdEos-CENPA-C-22

    Plasmid
    #57597
    Purpose
    Localization: Nucleus, Excitation: 505 / 569, Emission: 516 / 581
    Depositor
    Michael Davidson
    Insert
    CENPA (CENPA Human)
    Tags
    tdEos
    Expression
    Mammalian
    Mutation
    Please see Depositor Comments
    Promoter
    CMV
    Available since
    Jan. 29, 2015
    Availability
    Industry, Academic Institutions, and Nonprofits
    57597-plasmid-map-sequence-id-228931

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  20. Cwc25-pRSETA

    Plasmid
    #61043
    Purpose
    Encodes the yeast Cwc25 protein for overexpression and purification using a C-terminal hexahistidine tag. A single cysteine residue is encoded after the Cwc25 sequence for fluorescent labeling.
    Depositor
    Nils Walter
    Publication
    Krishnan et al Nat Struct Mol Biol. 2013 Dec;20(12):145
    Insert
    Cwc25
    Tags
    TEV site, Histidine Tag
    Expression
    Bacterial
    Promoter
    T7
    Available since
    Jan. 27, 2015
    Availability
    Academic Institutions and Nonprofits only
Showing: 6481 - 6500 of 12189 results