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pJM595 ZF6x6-C intC-ZF1-ZF2-NLS-HA in TUPV2

Plasmid

#161569

Purpose

Inducible expression of intC-ZF1-ZF2-NLS-HA under the ZF6x6-C promoter

Depositor

Joshua Leonard

Article

Muldoon et al Sci Adv. 2021 Feb 19;7(8). pii: 7/8/eabe

Insert

intC-ZF1-ZF2-NLS-HA

Use

Synthetic Biology

Tags

HA

Expression

Mammalian

Promoter

ZF6x6-C

Available Since

Jan. 7, 2021

Availability

Academic Institutions and Nonprofits only

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pJM583 EF1α 3xFLAG-NLS-VP64-ZF6 in TUPV2

Plasmid

#161544

Purpose

Constitutive expression of 3xFLAG-NLS-VP64-ZF6 under the EF1α promoter

Depositor

Joshua Leonard

Article

Muldoon et al Sci Adv. 2021 Feb 19;7(8). pii: 7/8/eabe

Insert

3xFLAG-NLS-VP64-ZF6

Use

Synthetic Biology

Tags

3x-FLAG

Expression

Mammalian

Promoter

EF1α

Available Since

Jan. 7, 2021

Availability

Academic Institutions and Nonprofits only

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pJB005 CMV 3xFLAG-NLS-intC-ZF2

Plasmid

#161550

Purpose

Constitutive expression of 3xFLAG-NLS-intC-ZF2 under the CMV promoter

Depositor

Joshua Leonard

Article

Muldoon et al Sci Adv. 2021 Feb 19;7(8). pii: 7/8/eabe

Insert

3xFLAG-NLS-intC-ZF2

Use

Synthetic Biology

Tags

3x-FLAG

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 7, 2021

Availability

Academic Institutions and Nonprofits only

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pJB002 CMV 3xFLAG-NLS-DsDed-ZF1

Plasmid

#161532

Purpose

Constitutive expression ofCMV 3xFLAG-NLS-DsDed-ZF1 under the CMV promoter

Depositor

Joshua Leonard

Article

Muldoon et al Sci Adv. 2021 Feb 19;7(8). pii: 7/8/eabe

Insert

3xFLAG-NLS-DsDed-ZF1

Use

Synthetic Biology

Tags

3x-FLAG

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 7, 2021

Availability

Academic Institutions and Nonprofits only

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pJM503 EF1α 3xFLAG-NLS-ZF1 in TUPV2

Plasmid

#161534

Purpose

Constitutive expression of 3xFLAG-NLS-ZF1 under the EF1α promoter

Depositor

Joshua Leonard

Article

Muldoon et al Sci Adv. 2021 Feb 19;7(8). pii: 7/8/eabe

Insert

3xFLAG-NLS-ZF1

Use

Synthetic Biology

Tags

3x-FLAG

Expression

Mammalian

Promoter

EF1α

Available Since

Jan. 7, 2021

Availability

Academic Institutions and Nonprofits only

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pJM506 EF1α 3xFLAG-NLS-DsDed-ZF1 in TUPV2

Plasmid

#161536

Purpose

Constitutive expression of 3xFLAG-NLS-DsDed-ZF1 gene under the EF1α promoter

Depositor

Joshua Leonard

Article

Muldoon et al Sci Adv. 2021 Feb 19;7(8). pii: 7/8/eabe

Insert

3xFLAG-NLS-DsDed-ZF1

Use

Synthetic Biology

Tags

3x-FLAG

Expression

Mammalian

Promoter

EF1α

Available Since

Jan. 7, 2021

Availability

Academic Institutions and Nonprofits only

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pJM507 EF1α 3xFLAG-NLS-DsDed-ZF10 in TUPV2

Plasmid

#161537

Purpose

Constitutive expression of 3xFLAG-NLS-DsDed-ZF10 under the EF1α promoter

Depositor

Joshua Leonard

Article

Muldoon et al Sci Adv. 2021 Feb 19;7(8). pii: 7/8/eabe

Insert

3xFLAG-NLS-DsDed-ZF10

Use

Synthetic Biology

Tags

3x-FLAG

Expression

Mammalian

Promoter

EF1α

Available Since

Jan. 7, 2021

Availability

Academic Institutions and Nonprofits only

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pJM512 EF1α 3xFLAG-NLS-VP64-ZF1 in TUPV2

Plasmid

#161538

Purpose

Constitutive expression of 3xFLAG-NLS-VP64-ZF1 under the EF1α promoter

Depositor

Joshua Leonard

Article

Muldoon et al Sci Adv. 2021 Feb 19;7(8). pii: 7/8/eabe

Insert

3xFLAG-NLS-VP64-ZF1

Use

Synthetic Biology

Tags

3x-FLAG

Expression

Mammalian

Promoter

EF1α

Available Since

Jan. 7, 2021

Availability

Academic Institutions and Nonprofits only

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pJM520 EF1α 3xFLAG-NLS-VP64-ZF10 in TUPV4

Plasmid

#161539

Purpose

Constitutive expression of 3xFLAG-NLS-VP64-ZF10 under the EF1α promoter

Depositor

Joshua Leonard

Article

Muldoon et al Sci Adv. 2021 Feb 19;7(8). pii: 7/8/eabe

Insert

3xFLAG-NLS-VP64-ZF10

Use

Synthetic Biology

Tags

3x-FLAG

Expression

Mammalian

Promoter

EF1α

Available Since

Jan. 7, 2021

Availability

Academic Institutions and Nonprofits only

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pJM574 ZF2x6-C 3xFLAG-NLS-DsDed-ZF1 in TUPV2

Plasmid

#161541

Purpose

Inducible expression of 3xFLAG-NLS-DsDed-ZF1 under the ZF2x6-C promoter

Depositor

Joshua Leonard

Article

Muldoon et al Sci Adv. 2021 Feb 19;7(8). pii: 7/8/eabe

Insert

3xFLAG-NLS-DsDed-ZF1

Use

Synthetic Biology

Tags

3x-FLAG

Expression

Mammalian

Promoter

ZF2x6-C

Available Since

Jan. 7, 2021

Availability

Academic Institutions and Nonprofits only

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pCDF-NtXNtS1HR1AtR2NtB2

Plasmid

#160890

Purpose

Expression of N. tabacum Rubisco small subunit (rbcS-SH1), Rubisco chaperone protiens rbcX, raf1, bsd2 and A. thaliana Rubisco chaperone proteins raf2 in E. coli.

Depositor

Maureen Hanson

Article

Lin et al Nat Plants. 2020 Oct;6(10):1289-1299. do

Insert

PT7-Nt-rbcS-S1H

Tags

6xHis

Expression

Bacterial

Promoter

rbcS-SH1: T7, chaperones: T13

Available Since

Dec. 4, 2020

Availability

Academic Institutions and Nonprofits only

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pCDF-NtXNtT4aR1AtR2NtB2

Plasmid

#160887

Purpose

Expression of N. tabacum Rubisco small subunit (rbcS-T4a), Rubisco chaperone protiens rbcX, raf1, bsd2 and A. thaliana Rubisco chaperone proteins raf2 in E. coli.

Depositor

Maureen Hanson

Article

Lin et al Nat Plants. 2020 Oct;6(10):1289-1299. do

Insert

PT7-Nt-rbcS-T4a

Expression

Bacterial

Promoter

rbcS-T4a: T7, chaperones: T10

Available Since

Dec. 4, 2020

Availability

Academic Institutions and Nonprofits only

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pKH-1699

Plasmid

#124227

Purpose

Mammalian SpCas9-HF1 expression

Depositor

Ervin Welker

Article

Talas et al Nucleic Acids Res. 2021 Jan 15. pii: 610

Insert

SpCas9-HF1

Use

CRISPR

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 23, 2020

Availability

Academic Institutions and Nonprofits only

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pZA44

Plasmid

#158522

Purpose

In planta gene expression of Golden gate compatible A. thaliana ZAR1 D498V/L17E-eGFP. Km plant selection marker.

Depositor

Sophien Kamoun

Article

Harant et al Plant Physiol. 2022 Jan 20;188(1):70-80.

Insert

ZAR1-eGFP (ZAR1 Mustard Weed)

Tags

eGFP

Expression

Plant

Mutation

L17E/D489V

Available Since

Sept. 25, 2020

Availability

Academic Institutions and Nonprofits only

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pUC_h7SK M15 (UUA)

Plasmid

#154785

Purpose

plasmid with single copy of Mma PylT M15 mutant (UUA anticodon)

Depositor

Simon Elsaesser

Article

Meineke et al Cell Rep. 2020 Jun 23;31(12):107811. doi

Insert

Mma PylT

Expression

Mammalian

Mutation

UUA anticodon and G10A, G14U, G16U, C20U, C52A

Promoter

h7SK

Available Since

Sept. 16, 2020

Availability

Academic Institutions and Nonprofits only

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pZA18

Plasmid

#158496

Purpose

In planta gene expression of Golden gate compatibleA. thaliana ZAR1 D489V. Km plant selection marker.

Depositor

Sophien Kamoun

Article

Harant et al Plant Physiol. 2022 Jan 20;188(1):70-80.

Insert

ZAR1 (ZAR1 Mustard Weed)

Expression

Plant

Mutation

D489V

Available Since

Sept. 11, 2020

Availability

Academic Institutions and Nonprofits only

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pZA36

Plasmid

#158514

Purpose

In planta gene expression of Golden gate compatible A. thaliana ZAR1 D498V/L17E-6xHA. Km plant selection marker.

Depositor

Sophien Kamoun

Article

Harant et al Plant Physiol. 2022 Jan 20;188(1):70-80.

Insert

ZAR1-6xHA (ZAR1 Mustard Weed)

Tags

6xHA

Expression

Plant

Mutation

L17E, D489V

Available Since

Sept. 4, 2020

Availability

Academic Institutions and Nonprofits only

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pZA19

Plasmid

#158497

Purpose

In planta gene expression of Golden gate compatlible A. thaliana ZAR1 L17E. Km plant selection marker.

Depositor

Sophien Kamoun

Article

Harant et al Plant Physiol. 2022 Jan 20;188(1):70-80.

Insert

ZAR1 (ZAR1 Mustard Weed)

Expression

Plant

Mutation

L17E

Available Since

Aug. 26, 2020

Availability

Academic Institutions and Nonprofits only

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pZA35

Plasmid

#158513

Purpose

In planta gene expression of Golden gate compatible A. thaliana ZAR1 L17E-6xHA. Km plant selection marker.

Depositor

Sophien Kamoun

Article

Harant et al Plant Physiol. 2022 Jan 20;188(1):70-80.

Insert

ZAR1-6xHA (ZAR1 Mustard Weed)

Tags

6xHA

Expression

Plant

Mutation

L17E

Available Since

Aug. 26, 2020

Availability

Academic Institutions and Nonprofits only

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pZA33

Plasmid

#158511

Purpose

In planta gene expression of Golden gate compatible A. thaliana ZAR1-6xHA. Km plant selection marker.

Depositor

Sophien Kamoun

Article

Harant et al Plant Physiol. 2022 Jan 20;188(1):70-80.

Insert

ZAR1-6xHA (ZAR1 Mustard Weed)

Tags

6xHA

Expression

Plant

Available Since

Aug. 26, 2020

Availability

Academic Institutions and Nonprofits only

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pZA20

Plasmid

#158498

Purpose

In planta gene expression of Golden gate complatible A. thaliana ZAR1 D498V/L17E. Km plant selection marker.

Depositor

Sophien Kamoun

Article

Harant et al Plant Physiol. 2022 Jan 20;188(1):70-80.

Insert

ZAR1 (ZAR1 Mustard Weed)

Expression

Plant

Mutation

L17E, D489V

Available Since

Aug. 26, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pZA41

Plasmid

#158519

Purpose

In planta gene expression of Golden gate compatible A. thaliana ZAR1-eGFP. Km plant selection marker.

Depositor

Sophien Kamoun

Article

Harant et al Plant Physiol. 2022 Jan 20;188(1):70-80.

Insert

ZAR1-eGFP (ZAR1 Mustard Weed)

Tags

eGFP

Expression

Plant

Available Since

Aug. 26, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pZA43

Plasmid

#158521

Purpose

In planta gene expression of Golden gate compatible A. thaliana ZAR1 L17E-eGFP. Km plant selection marker.

Depositor

Sophien Kamoun

Article

Harant et al Plant Physiol. 2022 Jan 20;188(1):70-80.

Insert

ZAR1-eGFP (ZAR1 Mustard Weed)

Tags

eGFP

Expression

Plant

Mutation

L17E

Available Since

Aug. 26, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pZA42

Plasmid

#158520

Purpose

In planta gene expression of Golden gate compatible A. thaliana ZAR1 D498V-eGFP. Km plant selection marker.

Depositor

Sophien Kamoun

Article

Harant et al Plant Physiol. 2022 Jan 20;188(1):70-80.

Insert

ZAR1-eGFP (ZAR1 Mustard Weed)

Tags

eGFP

Expression

Plant

Mutation

D489V

Available Since

Aug. 26, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pZA34

Plasmid

#158512

Purpose

In planta gene expression of Golden gate compatible A. thaliana ZAR1 D498V-6xHA. Km plant selection marker.

Depositor

Sophien Kamoun

Article

Harant et al Plant Physiol. 2022 Jan 20;188(1):70-80.

Insert

ZAR1-6xHA (ZAR1 Mustard Weed)

Tags

6xHA

Expression

Plant

Mutation

D489V

Available Since

Aug. 26, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pZA28

Plasmid

#158506

Purpose

In planta gene expression of Golden gate compatible A. thaliana ZAR1 D498V/L17E-4xMyc. Km plant selection marker.

Depositor

Sophien Kamoun

Article

Harant et al Plant Physiol. 2022 Jan 20;188(1):70-80.

Insert

ZAR1-4xMyc (ZAR1 Mustard Weed)

Tags

4xMyc

Expression

Plant

Mutation

L17E, D489V

Available Since

Aug. 26, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pZA25

Plasmid

#158503

Purpose

In planta gene expression of Golden gate compatible A. thaliana ZAR1-4xMyc. Km plant selection marker.

Depositor

Sophien Kamoun

Article

Harant et al Plant Physiol. 2022 Jan 20;188(1):70-80.

Insert

ZAR1-4xMyc (ZAR1 Mustard Weed)

Tags

4xMyc

Expression

Plant

Available Since

Aug. 26, 2020

Availability

Academic Institutions and Nonprofits only

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pAM-AAV-mIsl2-EGPF

Plasmid

#153191

Purpose

Mouse Isl2 (mIsl2) promoter-mediates gene expression in retinal neurons with fluorescent reporter

Depositor

Yang Hu

Article

Wang et al J Neurosci. 2020 Apr 9. pii: JNEUROSCI.0

Type

Empty backbone

Use

AAV

Expression

Mammalian

Available Since

June 26, 2020

Availability

Academic Institutions and Nonprofits only

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MTK0_004

Plasmid

#123961

Purpose

Encodes the Cas9 cROSA26 homology destination with Blasticidin resistance cassette GFP dropout destination vector as a type 0 part to be used in the MTK system

Depositor

Hana El-Samad

Article

Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

Insert

cRosa26 destination

Expression

Mammalian

Available Since

Feb. 17, 2020

Availability

Academic Institutions and Nonprofits only

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BII-C3H2B

Plasmid

#133387

Purpose

Piggybac vector for SRIRACCHA-mediated mutation enrichment (surrogate target for HDR)

Depositor

Blair Madison

Article

Wen et al J Biol Chem. 2017 Apr 14;292(15):6148-61

Type

Empty backbone

Expression

Mammalian

Promoter

CMV enhancer and hEf1a (CpG free)

Available Since

Jan. 3, 2020

Availability

Academic Institutions and Nonprofits only

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