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pGL3-Tet1 promoter B

Plasmid

#63882

Purpose

Luciferase reporter plasmid used to study transcriptional control of murine Tet1

Depositor

Kian Peng Koh

Article

Sohni et al Mol Cell Biol. 2015 Mar 15;35(6):1026-42

Insert

Tet1 promoter fragment B

Use

Luciferase

Expression

Mammalian

Available Since

June 26, 2015

Availability

Academic Institutions and Nonprofits only

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non-targeting control gRNA (BRDN0001162235)

Plasmid

#80261

Purpose

3rd generation lentiviral gRNA plasmid, non-targeting control gRNA

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

Non-targeting control gRNA

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

Aug. 30, 2016

Availability

Academic Institutions and Nonprofits only

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PIKFYVE gRNA (BRDN0001148312)

Plasmid

#76893

Purpose

3rd generation lentiviral gRNA plasmid targeting human PIKFYVE

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

PIKFYVE (PIKFYVE Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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pB-RTP1S-Integration

Plasmid

#129458

Purpose

piggyBac transposon vector that Inducibly (Tet-On) expresses RTP1S

Depositor

Sriram Kosuri

Article

Jones et al Cell Syst. 2019 Mar 27;8(3):254-260.e6.

Insert

RTP1 (Rtp1 Mouse)

Use

Synthetic Biology

Expression

Mammalian

Mutation

Removal of the first 36 amino acids of the protein

Promoter

TRE (Tet-On)

Available Since

Aug. 28, 2019

Availability

Academic Institutions and Nonprofits only

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pLKO.1 shETV1-B

Plasmid

#74974

Purpose

shETV1 shRNA

Depositor

William Hahn

Article

Wang et al Cell Rep. 2017 Feb 7;18(6):1543-1557. do

Insert

ETV1 (ETV1 Human)

Use

RNAi

Expression

Mammalian

Available Since

May 10, 2017

Availability

Academic Institutions and Nonprofits only

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PPAPDC2

Plasmid

#155836

Purpose

For use in RBP tethering screen

Depositor

Eugene Yeo

Article

Luo et al Nat Struct Mol Biol. 2020 Oct;27(10):989

Insert

PPAPDC2 (PLPP6 Human)

Tags

V5-MCP

Expression

Mammalian

Mutation

none

Promoter

EF-1a

Available Since

Oct. 28, 2022

Availability

Academic Institutions and Nonprofits only

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non-targeting control gRNA (BRDN0001149469)

Plasmid

#80257

Purpose

3rd generation lentiviral gRNA plasmid, non-targeting control gRNA

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

Non-targeting control gRNA

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

Aug. 30, 2016

Availability

Academic Institutions and Nonprofits only

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PIP5K1C gRNA (BRDN0001145164)

Plasmid

#77667

Purpose

3rd generation lentiviral gRNA plasmid targeting human PIP5K1C

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

PIP5K1C (PIP5K1C Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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RBM38

Plasmid

#155899

Purpose

For use in RBP tethering screen

Depositor

Eugene Yeo

Article

Luo et al Nat Struct Mol Biol. 2020 Oct;27(10):989

Insert

RBM38 (RBM38 Human)

Tags

V5-MCP

Expression

Mammalian

Mutation

none

Promoter

EF-1a

Available Since

Nov. 4, 2022

Availability

Academic Institutions and Nonprofits only

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pEntry_HCoV-OC43-nsp1

Plasmid

#168931

Purpose

Gateway-compatible Entry vector

Depositor

Pascal Falter-Braun

Article

Weller et al G3 (Bethesda). 2023 Jul 5;13(7):jkad105.

Insert

HCoV-OC43-nsp1 (ORF1ab )

Use

Gateway-compatible entry vector

Available Since

July 5, 2023

Availability

Academic Institutions and Nonprofits only

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pEntry_HCoV-HKU1-nsp1

Plasmid

#168893

Purpose

Gateway-compatible Entry vector

Depositor

Pascal Falter-Braun

Article

Weller et al G3 (Bethesda). 2023 Jul 5;13(7):jkad105.

Insert

HCoV-HKU1-nsp1 (ORF1ab )

Use

Gateway-compatible entry vector

Available Since

July 5, 2023

Availability

Academic Institutions and Nonprofits only

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pEntry_SARS-CoV-1-nsp1

Plasmid

#168841

Purpose

Gateway-compatible Entry vector

Depositor

Pascal Falter-Braun

Article

Weller et al G3 (Bethesda). 2023 Jul 5;13(7):jkad105.

Insert

SARS-CoV-1-nsp1 (ORF1ab )

Use

Gateway-compatible entry vector

Available Since

July 5, 2023

Availability

Academic Institutions and Nonprofits only

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pCR8/GW/TOPO-SRF

Plasmid

#98618

Purpose

Gateway entry TOPO TA cloning vector for SRF

Depositor

Richard Harvey

Article

Bouveret et al Elife. 2015 Jul 6;4. doi: 10.7554/eLife.

Insert

SRF (Srf Mouse)

Use

Topo ta cloning vector

Available Since

July 21, 2017

Availability

Academic Institutions and Nonprofits only

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PC4_3dad1h

Plasmid

#70699

Purpose

Expresses S. cerevisiae DASH/Dam1 complex with C-terminal his-tag on dad1

Depositor

Stephen Harrison

Article

Miranda et al Nat Struct Mol Biol. 2005 Feb;12(2):138-

Insert

DASH / Dam1 complex (DAM1, ASK1, DUO1, SPC34, SPC19, DAD1, DAD2, DAD4, HSK3, DAD3 Budding Yeast)

Tags

his-tag on C-terminus of dad1

Expression

Bacterial

Promoter

T7

Available Since

Feb. 6, 2020

Availability

Academic Institutions and Nonprofits only

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pEntry_HCoV-NL63-nsp13

Plasmid

#168928

Purpose

Gateway-compatible Entry vector

Depositor

Pascal Falter-Braun

Article

Weller et al G3 (Bethesda). 2023 Jul 5;13(7):jkad105.

Insert

HCoV-NL63-nsp13 (ORF1ab )

Use

Gateway-compatible entry vector

Available Since

July 5, 2023

Availability

Academic Institutions and Nonprofits only

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pEntry_HCoV-NL63-nsp3

Plasmid

#168930

Purpose

Gateway-compatible Entry vector

Depositor

Pascal Falter-Braun

Article

Weller et al G3 (Bethesda). 2023 Jul 5;13(7):jkad105.

Insert

HCoV-NL63-nsp3 (ORF1ab )

Use

Gateway-compatible entry vector

Available Since

July 5, 2023

Availability

Academic Institutions and Nonprofits only

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pEntry_HCoV-OC43-nsp7

Plasmid

#168935

Purpose

Gateway-compatible Entry vector

Depositor

Pascal Falter-Braun

Article

Weller et al G3 (Bethesda). 2023 Jul 5;13(7):jkad105.

Insert

HCoV-OC43-nsp7 (ORF1ab )

Use

Gateway-compatible entry vector

Available Since

July 5, 2023

Availability

Academic Institutions and Nonprofits only

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pEntry_HCoV-OC43-nsp9

Plasmid

#168937

Purpose

Gateway-compatible Entry vector

Depositor

Pascal Falter-Braun

Article

Weller et al G3 (Bethesda). 2023 Jul 5;13(7):jkad105.

Insert

HCoV-OC43-nsp9 (ORF1ab )

Use

Gateway-compatible entry vector

Available Since

July 5, 2023

Availability

Academic Institutions and Nonprofits only

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pEntry_HCoV-OC43-nsp10

Plasmid

#168938

Purpose

Gateway-compatible Entry vector

Depositor

Pascal Falter-Braun

Article

Weller et al G3 (Bethesda). 2023 Jul 5;13(7):jkad105.

Insert

HCoV-OC43-nsp10 (ORF1ab )

Use

Gateway-compatible entry vector

Available Since

July 5, 2023

Availability

Academic Institutions and Nonprofits only

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pEntry_HCoV-OC43-nsp14

Plasmid

#168939

Purpose

Gateway-compatible Entry vector

Depositor

Pascal Falter-Braun

Article

Weller et al G3 (Bethesda). 2023 Jul 5;13(7):jkad105.

Insert

HCoV-OC43-nsp14 (ORF1ab )

Use

Gateway-compatible entry vector

Available Since

July 5, 2023

Availability

Academic Institutions and Nonprofits only

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