We narrowed to 12,024 results for: pcdna

1. NC10 pCDNA3.1 VENUS WTX T289A

   Plasmid

   #36965

   Depositor

   Randall Moon

   Article

   Camp et al J Biol Chem. 2012 Feb 24;287(9):6539-50.

   Insert

   WTX T289A (AMER1 Human)
   

   Use

   Tags

   Venus

   Expression

   Mammalian

   Mutation

   T289A

   Promoter

   CMV

   Available Since

   July 24, 2012

   Availability

   Academic Institutions and Nonprofits only

2. NC9 pCDNA3.1 VENUS WTX S286E

   Plasmid

   #36964

   Depositor

   Randall Moon

   Article

   Camp et al J Biol Chem. 2012 Feb 24;287(9):6539-50.

   Insert

   WTX S286E (AMER1 Human)
   

   Use

   Tags

   Venus

   Expression

   Mammalian

   Mutation

   S286E

   Promoter

   CMV

   Available Since

   July 24, 2012

   Availability

   Academic Institutions and Nonprofits only

3. pCDNA3 Flag Bmf (S74A,S77A)

   Plasmid

   #24262

   Depositor

   Roger Davis

   Article

   Lei et al Proc Natl Acad Sci U S A. 2003 Mar 4. 10

   Insert

   Bmf (S87A,S90A) (BMF Human)
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   changed Serine 74 to Alanine changed Serine 77 t…

   Promoter

   Available Since

   June 7, 2010

   Availability

   Academic Institutions and Nonprofits only

4. pCDNA3 Flag Bmf (S74D,S77D)

   Plasmid

   #24263

   Depositor

   Roger Davis

   Article

   Lei et al Proc Natl Acad Sci U S A. 2003 Mar 4. 10

   Insert

   Bmf (S87D,S90D) (BMF Human)
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   changed Serine 74 to Aspartic Acid changed Serin…

   Promoter

   Available Since

   June 7, 2010

   Availability

   Academic Institutions and Nonprofits only

5. pcDNA5/FRT/TO-Flag-Tipin-L195A

   Plasmid

   #23118

   Depositor

   Aziz Sancar

   Article

   Kemp et al J Biol Chem. 2010 May 28;285(22):16562-7

   Insert

   Tipin (TIPIN Human)
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Changed Leucine 195 to Alanine

   Promoter

   Available Since

   Feb. 8, 2010

   Availability

   Academic Institutions and Nonprofits only

6. 3501 pcDNA3 Bid p15 mIII.4

   Plasmid

   #8781

   Depositor

   Stanley Korsmeyer

   Article

   Wei et al Genes Dev 2000 Aug 15;14(16):2060-71.

   Insert

   Bid p15 mIII.4 (Bid Mouse)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   July 6, 2005

   Availability

   Academic Institutions and Nonprofits only

7. Kv7.4 (1-645, Δ368-492)/pcDNA3.1

   Plasmid

   #111454

   Purpose

   Electrophysiology in HEK cell

   Depositor

   Dan Minor

   Article

   Chang et al Neuron. 2018 Feb 21;97(4):836-852.e6. do

   Insert

   potassium voltage-gated channel subfamily KQT member 4 isoform a [Homo sapiens] (KCNQ4 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Kv7.4 (1-645, Δ368-492), C643A

   Promoter

   Available Since

   Availability

   Academic Institutions and Nonprofits only

   

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8. pcDNA3-FLAG-RNMT 121-476

   Plasmid

   #112707

   Purpose

   Expresses N-terminally FLAG-tagged human RNMT 121-476 in mammalian cells

   Depositor

   Daniel Schoenberg

   Article

   Trotman et al Nucleic Acids Res. 2017 Oct 13;45(18):10

   Insert

   RNMT (RNMT Human)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   deleted amino acids 2-120

   Promoter

   CMV

   Available Since

   Availability

   Academic Institutions and Nonprofits only

   

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9. pcDNA3.1-Cerulean-Epac2(R53A)-Venus

   Plasmid

   #138197

   Purpose

   FRET-based Epac2 activation sensor with R53A mutation in Epac2 coding sequence.

   Depositor

   Jin Zhang

   Article

   Herbst et al Chem Biol. 2011 Feb 25;18(2):243-51. doi

   Insert

   Cerulean-Epac2(R53A)-Venus (Rapgef4 Synthetic, Mouse)
   

   Use

   Tags

   Cerulean and Venus

   Expression

   Mammalian

   Mutation

   Contains arginine-to-alanine substitution at resi…

   Promoter

   CMV

   Available Since

   Availability

   Academic Institutions and Nonprofits only

   

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10. pcDNA3.1-Cerulean-Epac2(R466A)-Venus

    Plasmid

    #138199

    Purpose

    FRET-based Epac2 activation sensor with R466A mutation in Epac2 coding sequence.

    Depositor

    Jin Zhang

    Article

    Herbst et al Chem Biol. 2011 Feb 25;18(2):243-51. doi

    Insert

    Cerulean-Epac2(R466A)-Venus (Rapgef4 Synthetic, Mouse)
    

    Use

    Tags

    Cerulean and Venus

    Expression

    Mammalian

    Mutation

    Contains arginine-to-alanine substitution at resi…

    Promoter

    CMV

    Available Since

    Availability

    Academic Institutions and Nonprofits only

    

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11. pcDNA3.1-Cerulean-Epac2(L426W)-Venus

    Plasmid

    #138200

    Purpose

    FRET-based Epac2 activation sensor with L426W mutation in Epac2 coding sequence.

    Depositor

    Jin Zhang

    Article

    Herbst et al Chem Biol. 2011 Feb 25;18(2):243-51. doi

    Insert

    Cerulean-Epac2(L426W)-Venus (Rapgef4 Synthetic, Mouse)
    

    Use

    Tags

    Cerulean and Venus

    Expression

    Mammalian

    Mutation

    Contains leucine-to-tryptophan substitution at re…

    Promoter

    CMV

    Available Since

    Availability

    Academic Institutions and Nonprofits only

    

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12. pcDNA3-mCherry-PKCsub-RLuc8N-NES

    Plasmid

    #138213

    Purpose

    Encodes substrate fragment of luminescence-based bimolecular PKC activity reporter (LumCKAR); cytosol targeted. Use in conjunction with pcDNA3-RLuc8C-FHA1-NES.

    Depositor

    Jin Zhang

    Article

    Herbst et al J Am Chem Soc. 2011 Apr 20;133(15):5676-

    Insert

    mCherry-PKCsub-RLuc8N-NES
    

    Use

    Tags

    6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Availability

    Academic Institutions and Nonprofits only

    

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13. pcDNA3-mCherry-PKCsub(T/A)-RLuc8N-NES

    Plasmid

    #138299

    Purpose

    Encodes negative-control substrate fragment of luminescence-based bimolecular PKC activity reporter (LumCKAR); cytosol targeted. Use in conjunction with pcDNA3-RLuc8C-FHA1-NES.

    Depositor

    Jin Zhang

    Article

    Herbst et al J Am Chem Soc. 2011 Apr 20;133(15):5676-

    Insert

    mCherry-PKCsub(T/A)-RLuc8N-NES
    

    Use

    Tags

    6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…

    Expression

    Mammalian

    Mutation

    Target Thr residue in substrate domain mutated to…

    Promoter

    CMV

    Available Since

    Availability

    Academic Institutions and Nonprofits only

    

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14. pcDNA3-Lyn-FRB-DMVF-181-N

    Plasmid

    #87609

    Purpose

    FRB-tagged N-terminal Dronpa fragment for BiFC; localizes to plasma membrane.

    Depositor

    Jin Zhang

    Article

    Hertel et al Cell Rep. 2016 Jan 12;14(2):390-400. doi

    Insert

    Lyn-FRB-DMVF181-N
    

    Use

    Tags

    N-terminal targeting sequence from Lyn kinase

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Availability

    Academic Institutions and Nonprofits only

    

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15. pcDNA3-LifeAct-FRB-DMVF-181-N

    Plasmid

    #87610

    Purpose

    FRB-tagged N-terminal Dronpa fragment for BiFC; localizes to actin cytoskeleton.

    Depositor

    Jin Zhang

    Article

    Hertel et al Cell Rep. 2016 Jan 12;14(2):390-400. doi

    Insert

    LifeAct-FRB-DMVF181-N
    

    Use

    Tags

    17-aa sequence from Abp140 (aka "Lifeact&quo…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Availability

    Academic Institutions and Nonprofits only

    

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16. pcDNA5/FRT/TO - RBM28 Exon1-6 E5 WT minigene

    Plasmid

    #169273

    Purpose

    Mammalian vector for constitutive expression of RBM28 Exon 1-6 WT minigene

    Depositor

    Susan Baserga

    Article

    Bryant et al Proc Natl Acad Sci U S A. 2021 May 11;11

    Insert

    RBM28 Exon1-6 WT minigene (RBM28 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Availability

    Academic Institutions and Nonprofits only

    

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17. pcDNA5/FRT/TO - RBM28 Exon1-6 E5 GT>A minigene

    Plasmid

    #169274

    Purpose

    Mammalian vector for constitutive expression of RBM28 Exon 1-6 GT>A minigene

    Depositor

    Susan Baserga

    Article

    Bryant et al Proc Natl Acad Sci U S A. 2021 May 11;11

    Insert

    RBM28 Exon1-6 GT>A minigene (RBM28 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    NM_018077.2:c.(541+1_541+2delinsA)

    Promoter

    CMV

    Available Since

    Availability

    Academic Institutions and Nonprofits only

    

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18. pcDNA5/FRT/TO_CalR-SNAP-tag2-KDEL

    Plasmid

    #226517

    Purpose

    Mamalian cell expression of SNAP-tag2 in endoplasmic reticulum

    Depositor

    Kai Johnsson

    Article

    Kühn et al Nat Chem Biol. 2025 Jul 3. doi: 10.1038/

    Insert

    CalR-SNAP-tag2-KDEL
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Oct. 15, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. CiDr-VSP L223F-mCherry pcDNA3.1 (eVSP)

    Plasmid

    #140892

    Purpose

    Expresses modified Danio rerio VSP without phosphatase activity (inactive mutant of enhanced VSP) fused with mCherry in mammalian cells.

    Depositor

    Yasushi Okamura

    Article

    Kawanabe et al J Gen Physiol. 2020 May 4;152(5). pii: 1

    Insert

    CiDr-VSP L223F-mCherry (tpte, vsp Ciona intestinalis, Zebrafish)
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    changed 223L to F in Dr-VSP (zebrafish TPTE)

    Promoter

    Available Since

    April 30, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. pcDNA5-EGFP-NLS-P2AT2A-mCherry-PTS1

    Plasmid

    #87828

    Purpose

    High-efficient mammalian expression vector for co-expression of EGFP-tagged and mCherry-tagged proteins using P2AT2A. NLS and PTS1 can be replaced by proteins of interest.

    Depositor

    Andrea Musacchio

    Article

    Pan et al Elife. 2017 Jan 6;6. pii: e23352. doi: 1

    Inserts

    NLS
    PTS1
    

    Use

    Tags

    EGFP and mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    CMV promoter, tetracycline operator

    Available Since

    April 4, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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